SM Table 1: Assessment of total RNA quality as determined by A260:A280 and RNA integrity number (RIN) isolated from heart, liver and testis tissues of male C57BL/6 mice.

Mouse / p53 genotype / Tissue / A260:A280 / RIN / Mouse / p53 genotype / Tissue / A260:A280 / RIN
7A / Wild type / Testis / 2.16 / 9.7 / 2A / Heterozygous / Testis / 2.13 / 8.8
7A / Wild type / Heart / 2.11 / 8.0 / 2A / Heterozygous / Heart / 2.08 / 8.7
7A / Wild type / Liver / 2.10 / 8.1 / 2A / Heterozygous / Liver / 2.11 / 8.1
9A / Wild type / Testis / 2.15 / 9.3 / 3A / Heterozygous / Testis / 2.12 / 9.3
9A / Wild type / Heart / 1.48 / 9.2 / 3A / Heterozygous / Heart / 2.09 / 8.9
9A / Wild type / Liver / 2.08 / 8.2 / 3A / Heterozygous / Liver / 2.11 / 7.0
11A / Wild type / Testis / 2.15 / 9.1 / 4A / Heterozygous / Testis / 2.13 / 9.4
11A / Wild type / Heart / 2.11 / 8.7 / 4A / Heterozygous / Heart / 2.14 / 8.4
11A / Wild type / Liver / * / 7.7 / 4A / Heterozygous / Liver / 2.13 / 7.9
8A / Wild type / Testis / 2.13 / 9.3 / 16A / Null / Testis / 2.13 / 9.3
8A / Wild type / Heart / 2.06 / 8.7 / 16A / Null / Heart / 2.06 / 8.0
8A / Wild type / Liver / 2.12 / 8.3 / 16A / Null / Liver / * / 7.1
10A / Wild type / Testis / 2.13 / 9.3 / 17A / Null / Testis / 2.18 / 9.2
10A / Wild type / Heart / 2.11 / 8.0 / 17A / Null / Heart / 2.07 / 8.9
10A / Wild type / Liver / * / 8.4 / 17A / Null / Liver / * / 8.4
12A / Wild type / Testis / 2.13 / 9.8 / 18A / Null / Testis / 2.14 / 9.3
12A / Wild type / Heart / 2.11 / 8.6 / 18A / Null / Heart / 2.11 / 8.7
12A / Wild type / Liver / * / 8.0 / 18A / Null / Liver / * / 8.4
1A / Heterozygous / Testis / * / 9.1 / 13A / Null / Testis / 2.13 / 9.7
1A / Heterozygous / Heart / n/a / n/a / 13A / Null / Heart / 1.97 / 8.8
1A / Heterozygous / Liver / 2.11 / 7.6 / 13A / Null / Liver / * / **
5A / Heterozygous / Testis / * / 8.5 / 14A / Null / Testis / 2.13 / 9.3
5A / Heterozygous / Heart / 2.05 / 8.6 / 14A / Null / Heart / 2.12 / 8.5
5A / Heterozygous / Liver / 2.10 / 7.3 / 14A / Null / Liver / * / 7.9
6A / Heterozygous / Testis / * / 9.0 / 15A / Null / Testis / 2.13 / 9.1
6A / Heterozygous / Heart / n/a / n/a / 15A / Null / Heart / 2.08 / 8.4
6A / Heterozygous / Liver / 2.13 / 7.8 / 15A / Null / Liver / 2.07 / 8.0

n/a: This sample was not included in the analysis due to the poor quality of the total RNA.

* An A260:A280 reading was not available for this sample.

** A RIN was not obtained for this sampledue to a technical error during analysis.

SM Table 2.Apoptosis Functional Gene Group: Values represent differential gene expression in the heart as compared to liver (A) and the testis as compared to liver (B). The functional sub-groups are: 2a) Induction of apoptosis, 2b) anti-apoptotic genes, 2c) genes regulating apoptosis, 2d) genes related to apoptosis. Genes below the limit of detection are denoted as <LOD.

A. Heart vs. Liver
------/ B. Testis vs. Liver
------
2a) / Gene / WT Avg 2ΔΔCt / WT pvalue / HET Avg 2ΔΔCt / HET pvalue / KO Avg 2ΔΔCt / KO pvalue / WT Avg 2ΔΔCt / WT pvalue / HET Avg 2ΔΔCt / HET pvalue / KO Avg 2ΔΔCt / KO pvalue
Induction of Apoptosis / Atm / 1.27 / 0.2322 / 1.39 / 0.4312 / 1.64 / 0.1796 / 4.19 / 0.0003 / 3.39 / 0.0000 / 3.24 / 0.0004
Bax / 1.04 / 0.8158 / 1.13 / 0.544 / 1.11 / 0.8617 / 1.31 / 0.0274 / 1.17 / 0.2185 / 1.27 / 0.0883
Casp2 / <LOD / ** / 1.58 / 0.4443* / <LOD / ** / 13.55 / 0.0054* / 11.83 / 0.001* / 11.26 / 0.0099*
Dapk1 / 1.22 / 0.9657* / <LOD / ** / <LOD / ** / 0.31 / 0.0017* / <LOD / ** / <LOD / **
Foxo3 / 2.97 / 0.004 / 3.95 / 0.1744 / 4.40 / 0.0519 / 3.76 / 0.0000 / 4.14 / 0.0001 / 6.51 / 0.0001
Myc / 0.42 / 0.053 / 0.62 / 0.3062 / 1.05 / 0.592 / 0.58 / 0.0748 / 0.46 / 0.0912 / 0.82 / 0.2069
Pmaip1 / <LOD / ** / <LOD / ** / <LOD / ** / 18.15 / 0.0421* / 11.18 / 0.0001* / 13.52 / 0.0351*
Prkca / 1.15 / 0.9423 / 1.62 / 0.0273 / 1.20 / 0.6473 / 1.34 / 0.0031 / 1.37 / 0.0047 / 1.34 / 0.047
Pten / 1.18 / 0.0677 / 1.14 / 0.6932 / 1.33 / 0.6001 / 3.26 / 0.0012 / 3.44 / 0.0000 / 4.49 / 0.0013
Tnf / <LOD / ** / <LOD / ** / <LOD / ** / <LOD / ** / <LOD / ** / <LOD / **
Trp63 / <LOD / ** / 5.24 / 0.0026* / <LOD / ** / 26.93 / 0.0001* / 27.61 / 0.0000* / 30.06 / 0.0002*
2b) / Gene / WT Avg 2ΔΔCt / WT pvalue / HET Avg 2ΔΔCt / HET pvalue / KO Avg 2ΔΔCt / KO pvalue / WT Avg 2ΔΔCt / WT pvalue / HET Avg 2ΔΔCt / HET pvalue / KO Avg 2ΔΔCt / KO pvalue
Anti-Apoptotic / Bcl2 / 15.14 / 0.0001 / 21.48 / 0.0077 / 27.07 / 0.0007 / 3.83 / 0.0008 / 4.02 / 0.0001 / 7.68 / 0.0001
Birc5 / <LOD / ** / <LOD / ** / <LOD / ** / 93.87 / 0.0000* / 111.13 / 0.0000* / 86.25 / 0.0000*
Dapk1 / 1.22 / 0.9657* / <LOD / ** / <LOD / ** / 0.31 / 0.0017* / <LOD / ** / <LOD / **

*A tissue has one or more samples with a Ct value 32 for this gene.

** Both tissues have samples with a Ct value 32 for this gene.

SM Table 2 (Continued).

A. Heart vs. Liver
------/ B. Testis vs. Liver
------
2c)
Regulation of Apoptosis / Gene / WT Avg 2ΔΔCt / WT pvalue / HET Avg 2ΔΔCt / HET pvalue / KO Avg 2ΔΔCt / KO pvalue / WT Avg 2ΔΔCt / WT pvalue / HET Avg 2ΔΔCt / HET pvalue / KO Avg 2ΔΔCt / KO pvalue
Apaf1 / 4.17 / 0.0001 / 6.82 / 0.0235 / 4.07 / 0.0286 / 8.73 / 0.0001 / 9.02 / 0.0000 / 9.52 / 0.0002
Bid / 0.11 / 0.0001 / 0.11 / 0.0007 / 0.12 / 0.0002 / 0.95 / 0.466 / 0.84 / 0.1146 / 0.99 / 0.8351
Brca1 / <LOD / ** / 2.78 / 0.4453 / <LOD / ** / 320.92 / 0.0029 / 270.88 / 0.0003 / 381.50 / 0.0777
Btg2 / 9.60 / 0.0000 / 8.35 / 0.0267 / 12.13 / 0.001 / 2.66 / 0.0222 / 1.84 / 0.1019 / 3.48 / 0.0018
Casp9 / 0.36 / 0.0012 / 0.42 / 0.0204 / 0.29 / 0.0029 / 1.31 / 0.0266 / 1.12 / 0.0382 / 1.07 / 0.8906
Cradd / 0.72 / 0.0182 / 0.54 / 0.0246 / 0.55 / 0.0138 / 0.45 / 0.0003 / 0.37 / 0.0011 / 0.33 / 0.0003
Fadd / 0.92 / 0.6391 / 1.45 / 0.2386 / 1.29 / 0.3182 / 1.25 / 0.1952 / 1.18 / 0.4111 / 1.70 / 0.0191
Mcl1 / 1.16 / 0.3021 / 1.45 / 0.2327 / 0.86 / 0.4531 / 1.41 / 0.0107 / 1.24 / 0.0547 / 1.27 / 0.3016
Gene / WT Avg 2ΔΔCt / WT pvalue / HET Avg 2ΔΔCt / HET pvalue / KO Avg 2ΔΔCt / KO pvalue / WT Avg 2ΔΔCt / WT pvalue / HET Avg 2ΔΔCt / HET pvalue / KO Avg 2ΔΔCt / KO pvalue
2d) / Bag1 / 2.76 / 0.001 / 3.38 / 0.0004 / 3.55 / 0.0028 / 11.94 / 0.0001 / 15.93 / 0.0000 / 14.76 / 0.0000
Related to Apoptosis / Bnip3 / 0.66 / 0.0378 / 0.52 / 0.0334 / 0.42 / 0.0576 / 0.12 / 0.0007 / 0.10 / 0.0066 / 0.08 / 0.0053
E2f1 / 1.05 / 0.6601* / 0.81 / 0.0728* / 2.01 / 0.2953* / 13.66 / 0.0000 / 10.46 / 0.0000 / 14.28 / 0.0007
Fasl / <LOD / ** / <LOD / ** / <LOD / ** / 2.32 / 0.0012* / 2.38 / 0.0069* / <LOD / **
Nfkb1 / 1.57 / 0.0001 / 2.21 / 0.0022 / 1.64 / 0.0303 / 1.25 / 0.0758 / 1.53 / 0.009 / 1.57 / 0.0066
Sirt1 / 1.16 / 0.2512 / 1.18 / 0.6008 / 1.15 / 0.8378 / 13.11 / 0.0000 / 11.40 / 0.0000 / 13.76 / 0.0002
Tnfrsf10b / 11.94 / 0.0782 / 13.45 / 0.0304* / <LOD / ** / 28.87 / 0.0713* / 25.67 / 0.0000* / 26.23 / 0.0003*
Traf1 / 2.57 / 0.0164 / 2.63 / 0.0784 / 3.60 / 0.287 / 72.71 / 0.0001 / 72.98 / 0.0000 / 83.63 / 0.0001
Trp53 / 1.07 / 0.6747 / 1.37 / 0.1043 / 1.62 / 0.245 / 2.93 / 0.0002 / 7.95 / 0.0000 / 58.73 / 0.0000
Trp53bp2 / 1.41 / 0.0766 / 1.62 / 0.5249 / 2.44 / 0.029 / 2.06 / 0.0073 / 1.97 / 0.0003 / 2.86 / 0.0007
Trp73 / <LOD / ** / <LOD / ** / <LOD / ** / 49.80 / 0.0000* / 42.97 / 0.0001* / 52.89 / 0.0017*

*A tissue has one or more samples with a Ct value 32 for this gene.

** Both tissues have samples with a Ct value 32 for this gene.

SM Table 3. Cell-Cycle Functional Gene Group: Values represent differential gene expression in the heart as compared to liver (A) and the testis as compared to liver (B). The functional sub-groups are: 3a) cell cycle arrest, 3b) negative regulation of the cell cycle, 3c) regulation of the cell cycle and 3d) genes related to the cell cycle. Genes below the limit of detection are denoted as <LOD.

A. Heart vs. Liver
------/ B. Testis vs. Liver
------
3a) / Gene / WT Avg 2ΔΔCt / WT pvalue / HET Avg 2ΔΔCt / HET pvalue / KO Avg 2ΔΔCt / KO pvalue / WT Avg 2ΔΔCt / WT pvalue / HET Avg 2ΔΔCt / HET pvalue / KO Avg 2ΔΔCt / KO pvalue
Cell-Cycle Arrest / Cdkn1a / 12.78 / 0.0286 / 25.05 / 0.1462 / 51.84 / 0.1406* / 21.08 / 0.0008 / 37.35 / 0.0000 / 86.99 / 0.0191*
Cdkn2a / <LOD / ** / 0.94 / 0.844* / <LOD / ** / 111.30 / 0.0000* / 93.91 / 0.0000 / 118.67 / 0.0003*
Chek1 / <LOD / ** / 1.97 / 0.2522 / <LOD / ** / 151.08 / 0.0007* / 112.45 / 0.0000 / 141.87 / 0.0112*
Gadd45a / 1.59 / 0.5084 / 1.29 / 0.2943 / 1.44 / 0.0259 / 1.29 / 0.7612 / 0.74 / 0.012 / 1.14 / 0.7831
Msh2 / 1.94 / 0.0654 / 1.41 / 0.0264 / 1.39 / 0.2856 / 3.36 / 0.0000 / 3.19 / 0.0000 / 3.02 / 0.0000
Rprm / <LOD / ** / <LOD / ** / <LOD / ** / <LOD / ** / <LOD / ** / <LOD / **
Sesn2 / 1.03 / 0.7835 / 1.45 / 0.6365 / 2.06 / 0.112 / 21.68 / 0.0000 / 23.16 / 0.0000 / 32.38 / 0.0000
3b) / Gene / WT Avg 2ΔΔCt / WT pvalue / HET Avg 2ΔΔCt / HET pvalue / KO Avg 2ΔΔCt / KO pvalue / WT Avg 2ΔΔCt / WT pvalue / HET Avg 2ΔΔCt / HET pvalue / KO Avg 2ΔΔCt / KO pvalue
Negative Regulation of the Cell-Cycle / Atm / 1.27 / 0.2322 / 1.39 / 0.4312 / 1.64 / 0.1796 / 4.19 / 0.0003 / 3.39 / 0.0000 / 3.24 / 0.0004
Brca1 / <LOD / ** / 2.78 / 0.4453* / <LOD / ** / 320.92 / 0.0029* / 270.88 / 0.0003* / 381.50 / 0.0777*
Pten / 1.18 / 0.0677 / 1.14 / 0.6932 / 1.33 / 0.6001 / 3.26 / 0.0012 / 3.44 / 0.0000 / 4.49 / 0.0013
Rb1 / 3.47 / 0.0021 / 2.67 / 0.1813 / 3.62 / 0.0151 / 6.02 / 0.0137 / 4.61 / 0.0000 / 5.52 / 0.003
Trp53 / 1.07 / 0.6747 / 1.37 / 0.1043 / 1.62 / 0.245 / 2.93 / 0.0002 / 7.95 / 0.0000 / 58.73 / 0.0000
Trp63 / <LOD / ** / 5.24 / 0.0026* / <LOD / ** / 26.93 / 0.0001* / 27.61 / 0.0000* / 30.06 / 0.0002*
Trp73 / <LOD / ** / <LOD / ** / <LOD / ** / 49.80 / 0.0000* / 42.97 / 0.0001* / 52.89 / 0.0017*
Wt1 / 541.09 / 0.0334* / 488.73 / 0.2823* / <LOD / ** / 541.09 / 0.0222* / 488.73 / 0.0037* / 570.78 / 0.0021*

*A tissue has one or more samples with a Ct value 32 for this gene.

** Both tissues have samples with a Ct value 32 for this gene.

SM Table 3 (Continued)

A. Heart vs. Liver
------/ B. Testis vs. Liver
------
3c) / Gene / WT Avg 2ΔΔCt / WT pvalue / HET Avg 2ΔΔCt / HET pvalue / KO Avg 2ΔΔCt / KO pvalue / WT Avg 2ΔΔCt / WT pvalue / HET Avg 2ΔΔCt / HET pvalue / KO Avg 2ΔΔCt / KO pvalue
Regulation of the Cell-Cycle / Brca2 / 1.04 / 0.986 / 1.20 / 0.5627 / 1.25 / 0.3504 / 84.82 / 0.0000 / 79.36 / 0.0000 / 80.45 / 0.0000
Ccne2 / 1.89 / 0.0091 / 1.59 / 0.5018 / 1.31 / 0.6372 / 73.95 / 0.0000 / 54.39 / 0.0000 / 37.38 / 0.0001
Cdk4 / 0.88 / 0.0563 / 1.10 / 0.7037 / 0.76 / 0.2535 / 2.51 / 0.0007 / 2.29 / 0.0012 / 2.44 / 0.0055
E2f1 / 1.05 / 0.6601* / 0.81 / 0.0728* / 2.01 / 0.2953* / 13.66 / 0.0000 / 10.46 / 0.0000 / 14.28 / 0.0007
E2f3 / 0.53 / 0.0723 / 0.83 / 0.2001 / 0.55 / 0.1559 / 1.66 / 0.2396 / 1.53 / 0.0002 / 1.66 / 0.0066
Esr1 / 0.08 / 0.036* / 0.06 / 0.052* / 0.08 / 0.0046* / 2.06 / 0.049 / 1.26 / 0.2677 / 1.31 / 0.2484
Jun / 11.19 / 0.0002 / 10.68 / 0.0317 / 22.91 / 0.0006 / 4.12 / 0.0018 / 3.99 / 0.002 / 6.93 / 0.0001
Kras / 0.34 / 0.0084 / 0.27 / 0.0068 / 0.18 / 0.0078 / 0.80 / 0.1168 / 0.62 / 0.0065 / 0.56 / 0.0308
Sfn / 4.74 / 0.0021 / 7.91 / 0.0474 / 7.21 / 0.0133 / 8.35 / 0.0002 / 6.77 / 0.0000 / 10.80 / 0.0000
3d) / Gene / WT Avg 2ΔΔCt / WT pvalue / HET Avg 2ΔΔCt / HET pvalue / KO Avg 2ΔΔCt / KO pvalue / WT Avg 2ΔΔCt / WT pvalue / HET Avg 2ΔΔCt / HET pvalue / KO Avg 2ΔΔCt / KO pvalue
Related to the Cell-Cycle / Ccnb2 / <LOD / ** / <LOD / ** / <LOD / ** / 62.25 / 0.0007* / 56.00 / 0.0000* / 55.92 / 0.0125*
Ccng1 / 30.65 / 0.0000 / 32.92 / 0.0013 / 32.73 / 0.0006 / 1.03 / 0.8316 / 0.94 / 0.3553 / 0.86 / 0.2349
Ccng2 / 2.42 / 0.0016 / 1.80 / 0.1299 / 2.22 / 0.019 / 2.88 / 0.0001 / 2.46 / 0.0003 / 2.92 / 0.0014
Ccnh / 2.01 / 0.0011 / 1.78 / 0.1777 / 2.02 / 0.0759 / 4.41 / 0.0003 / 4.20 / 0.0001 / 4.05 / 0.001
Cdc25a / 2.01 / 0.0001 / 2.70 / 0.0215 / 1.94 / 0.824 / 69.31 / 0.0000 / 109.04 / 0.0000 / 68.42 / 0.0000
Cdc25c / <LOD / ** / <LOD / ** / <LOD / ** / 119.51 / 0.0000* / 169.97 / 0.0002* / 127.24 / 0.0000*
Cdc2a / 0.56 / 0.009 / 0.55 / 0.1099 / 0.43 / 0.0257 / 64.69 / 0.0000 / 57.59 / 0.0000 / 62.73 / 0.0000
Chek2 / 1.03 / 0.6888* / 0.84 / 0.7227 / 1.66 / 0.8418* / 3.44 / 0.0023 / 2.50 / 0.0016 / 2.59 / 0.0336
Mdm2 / 1.49 / 0.0364 / 1.73 / 0.2461 / 1.19 / 0.9071 / 17.51 / 0.0001 / 17.68 / 0.0000 / 18.35 / 0.0014
Mlh1 / 0.90 / 0.604 / 0.97 / 0.8056 / 0.78 / 0.4269 / 3.65 / 0.0000 / 4.18 / 0.0000 / 4.75 / 0.0005
Cul9 / 5.82 / 0.0013 / 14.92 / 0.0127 / 8.59 / 0.0114 / 52.72 / 0.0000 / 89.08 / 0.0000 / 118.49 / 0.0000
Pcna / 0.47 / 0.005 / 0.47 / 0.0301 / 0.31 / 0.0034 / 9.87 / 0.0000 / 8.82 / 0.0000 / 7.89 / 0.0000
Ppm1d / 4.93 / 0.0001 / 4.40 / 0.0833 / 7.30 / 0.006 / 203.66 / 0.0000 / 252.22 / 0.0000 / 330.75 / 0.0000
Prc1 / 4.50 / 0.0036 / 5.21 / 0.0916 / 3.22 / 0.078 / 516.33 / 0.0001 / 621.55 / 0.0000 / 612.88 / 0.0001
Pttg1 / <LOD / ** / <LOD / ** / <LOD / ** / 85.52 / 0.0622* / 81.59 / 0.0000* / 114.93 / 0.001*
Trp53bp2 / 1.41 / 0.0766 / 1.62 / 0.5249 / 2.44 / 0.029 / 2.06 / 0.0073 / 1.97 / 0.0003 / 2.86 / 0.0007

*A tissue has one or more samples with a Ct value 32 for this gene.

** Both tissues have samples with a Ct value 32 for this gene.

SM Table 4. DNA Repair Functional Gene Group: Values represent differential gene expression in the heart as compared to liver (A) and the testis as compared to liver (B). Genes below the limit of detection are denoted as <LOD.

A. Heart vs. Liver
------/ B. Testis vs. Liver
------
Gene / WT Avg 2ΔΔCt / WT pvalue / HET Avg 2ΔΔCt / HET pvalue / KO Avg 2ΔΔCt / KO pvalue / WT Avg 2ΔΔCt / WT pvalue / HET Avg 2ΔΔCt / HET pvalue / KO Avg 2ΔΔCt / KO pvalue
DNA Repair / Apex1 / 0.72 / 0.0035 / 0.96 / 0.7444 / 0.85 / 0.264 / 2.86 / 0.0001 / 3.04 / 0.0000 / 3.70 / 0.0012
Atm / 1.27 / 0.2322 / 1.39 / 0.4312 / 1.64 / 0.1796 / 4.19 / 0.0003 / 3.39 / 0.0000 / 3.24 / 0.0004
Atr / 0.82 / 0.3091 / 0.76 / 0.3813 / 0.48 / 0.0774* / 84.52 / 0.004 / 65.25 / 0.0000 / 47.09 / 0.0175
Brca1 / <LOD / ** / 2.78 / 0.4453* / <LOD / ** / 320.92 / 0.0029* / 270.88 / 0.0003* / 381.50 / 0.0777*
Brca2 / 1.04 / 0.986 / 1.20 / 0.5627 / 1.25 / 0.3504 / 84.82 / 0.0000 / 79.36 / 0.0000 / 80.45 / 0.0000
Dnmt1 / <LOD / ** / <LOD / ** / <LOD / ** / 15.51 / 0.0113* / 14.81 / 0.0064* / 12.06 / 0.0096*
Ercc1 / 7.13 / 0.0001 / 7.28 / 0.0272 / 7.01 / 0.0038 / 20.80 / 0.0000 / 23.80 / 0.0000 / 28.10 / 0.0000
Lig4 / 0.63 / 0.0377 / 0.97 / 0.8853 / 0.68 / 0.3987* / 13.65 / 0.0002 / 13.11 / 0.0000 / 12.31 / 0.0029
Msh2 / 1.94 / 0.0654 / 1.41 / 0.0264 / 1.39 / 0.2856 / 3.36 / 0.0000 / 3.19 / 0.0000 / 3.02 / 0.0000
Pcna / 0.47 / 0.005 / 0.47 / 0.0301 / 0.31 / 0.0034 / 9.87 / 0.0000 / 8.82 / 0.0000 / 7.89 / 0.0000
Pttg1 / <LOD / ** / <LOD / ** / <LOD / ** / 85.52 / 0.0622* / 81.59 / 0.0000* / 114.93 / 0.001*
Rev3l / 0.86 / 0.2594 / 0.95 / 0.929 / 0.77 / 0.3165 / 27.39 / 0.0002 / 30.95 / 0.0000 / 36.55 / 0.0017
Xrcc4 / 1.66 / 0.0027 / 1.45 / 0.3859 / 1.63 / 0.2685 / 15.05 / 0.0000 / 15.86 / 0.0000 / 16.65 / 0.0000
Xrcc5 / 0.78 / 0.0594 / 0.72 / 0.1781 / 0.79 / 0.1636 / 2.75 / 0.001 / 2.42 / 0.0002 / 3.31 / 0.0004

*A tissue has one or more samples with a Ct value 32 for this gene.

** Both tissues have samples with a Ct value 32 for this gene.

SM Table 5. Effect of p53 alleles on gene expression for genes involved in apoptosis in heart, liver and testes.

Heart / Liver / Testes
------
HET vs. WT / ------
KO vs. WT / ------
HET vs. WT / ------
KO vs. WT / ------
HET vs. WT / ------
KO vs. WT
5a) / Gene / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value
Induction of apoptosis / Atm / 1.07 / 0.7186 / 1.51 / 0.9943 / 1.01 / 0.9943 / 1.42 / 0.1137 / 0.86 / 0.0142 / 0.96 / 0.351
Bax / 1.11 / 0.2895 / 0.98 / 0.9695 / 0.99 / 0.9695 / 1.05 / 0.6146 / 0.89 / 0.0137 / 0.88 / 0.0247
Casp2 / 0.88 / 0.3766* / <LOD / ** / <LOD / ** / <LOD / ** / 0.86 / 0.0583 / 1.03 / 0.6377
Dapk1 / <LOD / ** / <LOD / ** / 0.86 / 0.3528* / 0.94 / 0.4349 / <LOD / ** / <LOD / **
Foxo3 / 0.95 / 0.7365 / 0.93 / 0.6869 / 0.91 / 0.6869 / 0.75 / 0.3527 / 0.97 / 0.6199 / 1.05 / 0.1554
Myc / 1.04 / 0.8496 / 1.77 / 0.3495 / 1.3 / 0.3495 / 0.67 / 0.1881 / 0.84 / 0.133 / 1.17 / 0.2616
Pmaip1 / <LOD / ** / <LOD / ** / <LOD / ** / <LOD / ** / 0.73 / 0.1031 / 1.03 / 0.6901
Prkca / 1.47 / 0.0568 / 1.25 / 0.3523 / 0.88 / 0.3523 / 1.25 / 0.302 / 0.91 / 0.2401 / 1.07 / 0.452
Pten / 0.92 / 0.5996 / 0.9 / 0.2629 / 1.07 / 0.2629 / 1.01 / 0.7943 / 1.17 / 0.0465 / 1.2 / 0.0574
Tnf / <LOD / ** / <LOD / ** / <LOD / ** / <LOD / ** / <LOD / ** / <LOD / **
Trp63 / 1.24 / 0.9437* / <LOD / ** / <LOD / ** / <LOD / ** / 1.08 / 0.3528 / 1.41 / 0.0008
5b) / Gene / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value
Anti-Apoptotic / Bcl2 / 1.15 / 0.3849 / 1.14 / 0.2415 / 0.87 / 0.3935 / 0.75 / 0.2394 / 0.92 / 0.2544 / 1.3 / 0.002
Birc5 / <LOD / ** / <LOD / ** / <LOD / ** / <LOD / ** / 1.26 / 0.006 / 1.27 / 0.0037
Dapk1 / <LOD / ** / <LOD / ** / 0.86 / 0.3528* / 0.94 / 0.4349 / <LOD / ** / <LOD / **

*A tissue has one or more samples with a Ct value 32 for this gene.

** Both tissues have samples with a Ct value 32 for this gene.

SM Table 5(Continued).

Heart / Liver / Testes
------
HET vs. WT / ------
KO vs. WT / ------
HET vs. WT / ------
KO vs. WT / ------
HET vs. WT / ------
KO vs. WT
5c) / Gene / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value
Regulation of Apoptosis / Apaf1 / 1.13 / 0.3758 / 0.74 / 0.0758 / 0.86 / 0.5986 / 1.03 / 0.5986 / 0.87 / 0.0068 / 0.93 / 0.1317
Bid / 1.03 / 0.8475 / 1.17 / 0.2094* / 1.07 / 0.4029 / 1.12 / 0.4029 / 0.98 / 0.6625 / 1.05 / 0.1085
Brca1 / 0.86 / 0.4899* / <LOD / ** / <LOD / ** / <LOD / ** / 1.02 / 0.5804 / 1.36 / 0.0059
Btg2 / 0.99 / 0.9116 / 1.02 / 0.9211 / 1.36 / 0.9261 / 0.92 / 0.9261 / 0.95 / 0.8096 / 1.07 / 0.859
Casp9 / 1.33 / 0.016 / 0.97 / 0.9852 / 1.09 / 0.0696 / 1.4 / 0.0696 / 0.96 / 0.3437 / 0.99 / 0.7169
Cradd / 0.87 / 0.309 / 1.08 / 0.4821 / 1.09 / 0.0243 / 1.65 / 0.0243 / 0.91 / 0.1937 / 1.06 / 0.3719
Fadd / 1.66 / 0.2649 / 0.82 / 0.6821* / 1.01 / 0.1234 / 0.68 / 0.1234 / 0.96 / 0.7391 / 0.79 / 0.0175
Mcl1 / 1.36 / 0.0151 / 0.72 / 0.2163 / 1.05 / 0.1307 / 1.32 / 0.1307 / 0.94 / 0.112 / 0.98 / 0.9637
5d) / Gene / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value
Related to Apoptosis / Bag1 / 1.15 / 0.4361 / 1.29 / 0.2509 / 0.93 / 0.5028 / 1.09 / 0.5028 / 1.3 / 0.0576 / 1.22 / 0.1775
Bnip3 / 0.86 / 0.0394 / 0.96 / 0.7892 / 1.23 / 0.1362 / 1.79 / 0.1362 / 0.96 / 0.406 / 0.99 / 0.7976
E2f1 / <LOD / ** / <LOD / ** / 1.03 / 0.2492 / 1.27 / 0.2492 / 0.84 / 0.0057 / 1.14 / 0.1646
Fasl / <LOD / ** / <LOD / ** / <LOD / ** / <LOD / ** / 1.03 / 0.764* / 1.22 / 0.3426
Nfkb1 / 1.21 / 0.2633 / 0.87 / 0.0241 / 0.88 / 0.596 / 0.93 / 0.596 / 1.09 / 0.3531 / 1.02 / 0.8766
Sirt1 / 1.09 / 0.7632 / 0.98 / 0.9797 / 1.12 / 0.3282 / 1.17 / 0.3282 / 0.99 / 0.779 / 1.06 / 0.3219
Tnfrsf10b / 1.14 / 0.3068 / 0.86 / 0.5975* / <LOD / ** / <LOD / ** / 0.91 / 0.2278 / 1.06 / 0.451
Traf1 / 1.09 / 0.5398 / 1.31 / 0.4541* / 1.03 / 0.6842* / <LOD / ** / 1.12 / 0.138 / 1.16 / 0.0837
Trp53 / 0.57 / 0.0011 / 0.12 / 0.0011 / 0.45 / <0.001 / 0.11 / <0.001 / 1.26 / 0.0023 / 1.64 / <0.001
Trp53bp2 / 0.88 / 0.4693 / 1.31 / 0.2026 / 0.96 / 0.2992 / 0.87 / 0.2992 / 0.96 / 0.367 / 1.08 / 0.2433
Trp73 / <LOD / ** / <LOD / ** / <LOD / ** / <LOD / ** / 0.88 / 0.2943 / 1.29 / 0.1101

*A tissue has one or more samples with a Ct value 32 for this gene.

** Both tissues have samples with a Ct value 32 for this gene.

SM Table 6. Effect of p53 alleles on gene expression for genes involved in the cell-cycle in heart, liver and testes.

Heart / Liver / Testes
------
HET vs. WT / ------
KO vs. WT / ------
HET vs. WT / ------
KO vs. WT / ------
HET vs. WT / ------
KO vs. WT
6a) / Gene / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value
Cell-Cycle Arrest / Cdkn1a / 0.52 / 0.1993 / 1.51 / 0.5286 / 0.33 / 0.1871 / 0.17 / 0.2231* / 0.84 / 0.0426 / 0.73 / 0.0028
Cdkn2a / <LOD / ** / <LOD / ** / 1.02 / 0.5425* / <LOD / ** / 0.91 / 0.0713 / 0.9 / 0.0075
Chek1 / 1.37 / 0.1761* / <LOD / ** / 1.28 / 0.3113* / <LOD / ** / 1.00 / 0.9348 / 1.08 / 0.4347
Gadd45a / 1.25 / 0.1147 / 0.95 / 0.7037 / 1.37 / 0.3959 / 1.02 / 0.7592 / 0.85 / 0.0688 / 0.81 / 0.0088
Msh2 / 0.83 / 0.3488 / 0.83 / 0.4252 / 0.96 / 0.7142 / 1.23 / 0.2208 / 0.93 / 0.3216 / 0.97 / 0.6406
Rprm / <LOD / ** / <LOD / ** / <LOD / ** / <LOD / ** / <LOD / ** / <LOD / **
Sesn2 / 1.05 / 0.5755 / 1.16 / 0.3692 / 0.84 / 0.2734 / 0.73 / 0.1283 / 0.93 / 0.3047 / 0.95 / 0.3079
6b) / Gene / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value
Negative Regulation of the Cell-Cycle / Atm / 1.07 / 0.7186 / 1.51 / 0.1132 / 1.01 / 0.9943 / 1.42 / 0.1137 / 0.86 / 0.0142 / 0.96 / 0.351
Brca1 / 0.86 / 0.4899* / <LOD / ** / <LOD / ** / <LOD / ** / 1.02 / 0.5804 / 1.36 / 0.0059
Pten / 0.92 / 0.5996 / 0.9 / 0.5864 / 1.07 / 0.2629 / 1.01 / 0.7943 / 1.17 / 0.0465 / 1.2 / 0.0574
Rb1 / 0.84 / 0.4647 / 1.18 / 0.3366 / 1.09 / 0.4456 / 1.37 / 0.0873 / 1.02 / 0.6699 / 1.27 / 0.4272
Trp53 / 0.57 / 0.0011 / 0.12 / 0.0011 / 0.45 / 0.0001 / 0.11 / <0.001 / 1.26 / 0.0023 / 1.64 / <0.001
Trp63 / 1.24 / 0.9437* / <LOD / ** / <LOD / ** / <LOD / ** / 1.08 / 0.3528 / 1.41 / 0.0008
Trp73 / <LOD / ** / <LOD / ** / <LOD / ** / <LOD / ** / 0.88 / 0.2943 / 1.29 / 0.1101
Wt1 / 1.28 / 0.1201 / 0.77 / 0.4532* / <LOD / ** / <LOD / ** / 0.77 / 0.078 / 0.94 / 0.8298

*A tissue has one or more samples with a Ct value 32 for this gene.

** Both tissues have samples with a Ct value 32 for this gene.

SM Table 6 (Continued).

Heart / Liver / Testes
------
HET vs. WT / ------
KO vs. WT / ------
HET vs. WT / ------
KO vs. WT / ------
HET vs. WT / ------
KO vs. WT
6c) / Gene / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value
Regulation of the Cell-Cycle / Brca2 / 1.09 / 0.5619 / 1.41 / 0.1763 / 1.09 / 0.4928 / 1.33 / 0.1496 / 1.03 / 0.5358 / 1.1 / 0.0238
Ccne2 / 0.97 / 0.817 / 1.42 / 0.186 / 1.4 / 0.1564 / 2.32 / 0.0641 / 1.09 / 0.1328 / 1.03 / 0.4639
Cdk4 / 1.17 / 0.2183 / 0.84 / 0.4059 / 1.04 / 0.6588 / 1.19 / 0.2897 / 0.95 / 0.3803 / 0.98 / 0.8697
E2f1 / <LOD / ** / <LOD / ** / 1.03 / 0.9845 / 1.27 / 0.2492 / 0.84 / 0.0057 / 1.14 / 0.1646
E2f3 / 1.44 / 0.1901 / 0.96 / 0.2847* / 0.81 / 0.3062 / 0.97 / 0.7945 / 0.83 / 0.0159 / 0.9 / 0.0867
Esr1 / 0.79 / 0.2247 / <LOD / ** / 1.2 / 0.5074 / 1.79 / 0.0915 / 0.77 / 0.1248 / 0.99 / 0.9714
Jun / 1.01 / 0.9379 / 1.38 / 0.0666 / 1.1 / 0.5585 / 0.73 / 0.2117 / 1.02 / 0.7555 / 1.11 / 0.0744
Kras / 0.97 / 0.8964 / 0.81 / 0.3613 / 0.99 / 0.9744 / 1.7 / 0.0799 / 0.8 / 0.0475 / 0.98 / 0.8606
Sfn / 1.22 / 0.1165 / 1.05 / 0.8017 / 0.93 / 0.743 / 0.79 / 0.0313 / 0.76 / 0.0094 / 0.9 / 0.18
6d) / Gene / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value
Related to the Cell-Cycle / Ccnb2 / <LOD / ** / <LOD / ** / <LOD / ** / <LOD / ** / 0.99 / 0.7683 / 0.91 / 0.7707
Ccng1 / 1.06 / 0.6574 / 1.16 / 0.3323 / 0.94 / 0.8829 / 1.31 / 0.1553 / 0.87 / 0.0121 / 0.92 / 0.2962
Ccng2 / 0.91 / 0.2696 / 1.06 / 0.6522 / 1.19 / 0.3071 / 1.36 / 0.1948 / 1.03 / 0.5121 / 1.17 / 0.002
Ccnh / 0.94 / 0.6363 / 1.23 / 0.2151 / 1.11 / 0.3963 / 1.47 / 0.1106 / 1.08 / 0.2096 / 1.17 / 0.0344
Cdc25a / 1.08 / 0.5572 / 1.24 / 0.3294 / 0.81 / 0.0952 / 1.83 / 0.3201 / 1.29 / 0.0106 / 1.25 / 0.0128
Cdc25c / <LOD / ** / <LOD / ** / <LOD / ** / <LOD / ** / 1.49 / 0.0084 / 1.39 / 0.0034
Cdc2a / 1.17 / 0.5656 / 1.03 / 0.6178* / 1.2 / 0.287 / 1.41 / 0.1659 / 1.08 / 0.2904 / 1.1 / 0.0532
Chek2 / <LOD / ** / <LOD / ** / 1.06 / 0.6922 / 1.64 / 0.1317 / 0.82 / 0.0873 / 1 / 0.7462
Mdm2 / 1.17 / 0.3248 / 0.74 / 0.1561 / 1.1 / 0.3991 / 1.22 / 0.2632 / 1.1 / 0.1777 / 1.07 / 0.4552
Mlh1 / 0.92 / 0.5437 / 0.81 / 0.4281* / 0.81 / 0.0514 / 1.03 / 0.6806 / 0.93 / 0.0418 / 1.14 / 0.1004
Cul9 / 1.64 / 0.046 / 0.89 / 0.6418 / 0.76 / 0.4448 / 0.64 / 0.0678 / 1.17 / 0.0116 / 1.17 / 0.0002
Pcna / 1.25 / 0.3835 / 0.91 / 0.7771 / 1.21 / 0.2199 / 1.42 / 0.0972 / 1.07 / 0.3562 / 0.96 / 0.4675
Ppm1d / 0.84 / 0.1891 / 1.32 / 0.2346 / 1.02 / 0.9798 / 1.03 / 0.7551 / 1.34 / 0.0006 / 1.45 / <0.001
Prc1 / 1.2 / 0.3903 / 1.16 / 0.7404* / <LOD / ** / <LOD / ** / 1.12 / 0.0649 / 1.23 / <0.001
Pttg1 / <LOD / ** / <LOD / ** / <LOD / ** / <LOD / ** / 1.04 / 0.6014 / 1.48 / 0.0253
Trp53bp2 / 0.88 / 0.4693 / 1.31 / 0.2026 / 0.96 / 0.7454 / 0.87 / 0.2992 / 0.96 / 0.367 / 1.08 / 0.2433

*A tissue has one or more samples with a Ct value 32 for this gene.

** Both tissues have samples with a Ct value 32 for this gene.

SM Table 7. Effect of p53 alleles on gene expression for genes involved in DNA repair and transcription in heart, liver and testes.

Heart / Liver / Testes
------HET vs. WT / ------
KO vs. WT / ------
HET vs. WT / ------
KO vs. WT / ------
HET vs. WT / ------
KO vs. WT
Gene / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value / ΔΔCt / p-value
DNA Repair / Apex1 / 1.38 / 0.2001 / 1.08 / 0.699 / 1.01 / 0.7772 / 1 / 0.8686 / 1.09 / 0.0976 / 1.11 / 0.2211
Atm / 1.07 / 0.7186 / 1.51 / 0.1132 / 1.01 / 0.9943 / 1.42 / 0.1137 / 0.86 / 0.0142 / 0.96 / 0.351
Atr / 1.17 / 0.5841 / 0.99 / 0.9995* / 1.04 / 0.9644 / 2.15 / 0.0700 / 0.86 / 0.3271 / 0.93 / 0.7618
Brca1 / 0.86 / 0.4899* / <LOD / ** / <LOD / ** / <LOD / ** / 1.02 / 0.5804 / 1.36 / 0.0059
Brca2 / 1.09 / 0.5619 / 1.41 / 0.1763 / 1.09 / 0.4928 / 1.33 / 0.1496 / 1.03 / 0.5358 / 1.1 / 0.0238
Dnmt1 / <LOD / ** / <LOD / ** / <LOD / ** / <LOD / ** / 0.93 / 0.5011 / 0.89 / 0.5309
Ercc1 / 1.02 / 0.7872 / 0.9 / 0.7489 / 1 / 0.9958 / 1.03 / 0.6902 / 1.15 / 0.0661 / 1.21 / 0.0023
Lig4 / 1.82 / 0.006 / 1.13 / 0.8151* / 1.03 / 0.7512 / 1.41 / 0.046 / 1.02 / 0.8546 / 1.11 / 0.3695
Msh2 / 0.83 / 0.3488 / 0.83 / 0.4252 / 0.96 / 0.7142 / 1.23 / 0.2208 / 0.93 / 0.3216 / 1.14 / 0.6406
Pcna / 1.25 / 0.3835 / 0.91 / 0.7771 / 1.21 / 0.2199 / 1.42 / 0.0972 / 1.07 / 0.3562 / 0.96 / 0.4675
Pttg1 / <LOD / ** / <LOD / ** / <LOD / ** / <LOD / ** / 1.04 / 0.6014 / 1.48 / 0.0253
Rev3l / 0.99 / 0.9431 / 0.74 / 0.3373 / 0.94 / 0.4716 / 1.11 / 0.4752 / 1.09 / 0.3568 / 1.22 / 0.1421
Xrcc4 / 0.94 / 0.6925 / 1.2 / 0.2359 / 1.11 / 0.3183 / 1.5 / 0.0879 / 1.19 / 0.0121 / 1.45 / 0.0002
Xrcc5 / 1.05 / 0.7984 / 1 / 0.7038 / 1.18 / 0.0206 / 1.12 / 0.4005 / 1.06 / 0.3985 / 1.17 / 0.0204

*A tissue has one or more samples with a Ct value 32 for this gene.

** Both tissues have samples with a Ct value 32 for this gene.