Supplementary Table 2.The number of animals used in each experiment

(1)

Figure / Method / Group / Embryo/Pup / Pregnant mothers
1 / Electron microscope / Con Male
(Week 1) / One offspring was randomly selected from each litter. / Six litters (n = 6)
1 / Electron microscope / Con Female
(Week 1) / One offspring was randomly selected from each litter. / Six litters (n = 6)
1 / Electron microscope / VPA Male
(Week 1) / One offspring was randomly selected from each litter. / Six litters (n = 6)
1 / Electron microscope / VPA Female
(Week 1) / One offspring was randomly selected from each litter. / Six litters (n = 6)
1 / Electron microscope / Con Male
(Week 2) / Three offspring was randomly selected from each litter. / Six litters (n = 7)
1 / Electron microscope / Con Female
(Week 2) / Three offspring was randomly selected from each litter. / Six litters (n = 7)
1 / Electron microscope / VPA Male
(Week 2) / Three offspring was randomly selected from each litter. / Six litters (n = 7)
1 / Electron microscope / VPA Female
(Week 2) / Three offspring was randomly selected from each litter. / Six litters (n = 7)
1 / Electron microscope / Con Male
(Week 4) / Four offspring was randomly selected from each litter. / Six litters (n = 6)
1 / Electron microscope / Con Female
(Week 4) / Four offspring was randomly selected from each litter. / Six litters (n = 6)
1 / Electron microscope / VPA Male
(Week 4) / Four offspring was randomly selected from each litter. / Six litters (n = 6)
1 / Electron microscope / VPA Female
(Week 4) / Four offspring was randomly selected from each litter. / Six litters (n = 6)
2A,
S1A, S2A / Western blot / Con Male
(Week 1) / One offspring was randomly selected from each litter. / Four litters (n = 4)
2A,
S1A, S2A / Western blot / Con Female
(Week 1) / One offspring was randomly selected from each litter. / Four litters (n = 4)
2A,
S1A, S2A / Western blot / VPA Male
(Week 1) / One offspring was randomly selected from each litter. / Four litters (n = 4)
2A,
S1A, S2A / Western blot / VPA Female
(Week 1) / One offspring was randomly selected from each litter. / Four litters (n = 4)
2B,
S1B, S2B / Western blot / Con Male
(Week 2) / One offspring was randomly selected from each litter. / Four litters (n = 4)
2B,
S1B, S2B / Western blot / Con Female
(Week 2) / One offspring was randomly selected from each litter. / Four litters (n = 4)
2B,
S1B, S2B / Western blot / VPA Male
(Week 2) / One offspring was randomly selected from each litter. / Four litters (n = 4)
2B,
S1B, S2B / Western blot / VPA Female
(Week 2) / One offspring was randomly selected from each litter. / Four litters (n = 4)
2C,
S1C, S2C / Western blot / Con Male
(Week 4) / One offspring was randomly selected from each litter. / Four litters (n = 4)
2C,
S1C, S2C / Western blot / Con Female
(Week 4) / One offspring was randomly selected from each litter. / Four litters (n = 4)
2C,
S1C, S2C / Western blot / VPA Male
(Week 4) / One offspring was randomly selected from each litter. / Four litters (n = 4)
2C,
S1C, S2C / Western blot / VPA Female
(Week 4) / One offspring was randomly selected from each litter. / Four litters (n = 4)
3A / Western blot / Con Male
(E14) / Four embryos were randomly selected and pooled together from each litter. / Four litters (n = 4)
3A / Western blot / Con Female
(E14) / Four embryos were randomly selected and pooled together from each litter. / Four litters (n = 4)
3A / Western blot / VPA Male
(E14) / Four embryos were randomly selected and pooled together from each litter. / Four litters (n = 4)
3A / Western blot / VPA Female
(E14) / Four embryos were randomly selected and pooled together from each litter. / Four litters (n = 4)

(2)

Figure / Method / Group / Embryo/Pup / Pregnant mothers
3B / Western blot / Con Male NPC
(DIV1) / NPCs were cultured from 10 male embryos pooled together from each litter. / Four litters (n = 4)
3B / Western blot / Con Female NPC
(DIV1) / NPCs were cultured from 10 female embryos pooled together from each litter. / Four litters (n = 4)
3B / Western blot / VPA Male NPC
(DIV1, VPA 0.3 mM) / NPCs were cultured from 10 male embryos pooled together from each litter. / Four litters (n = 4)
3B / Western blot / VPA Female NPC
(DIV1, VPA 0.3 mM) / NPCs were cultured from 10 female embryos pooled together from each litter. / Four litters (n = 4)
3C / Western blot / Con Male NPC
(DIV7) / NPCs were cultured from 10 male embryos pooled together from each litter. / Four litters (n = 4)
3C / Western blot / Con Female NPC
(DIV7) / NPCs were cultured from 10 female embryos pooled together from each litter. / Four litters (n = 4)
3C / Western blot / VPA Male NPC
(DIV7, VPA 0.3 mM) / NPCs were cultured from 10 male embryos pooled together from each litter. / Four litters (n = 4)
3C / Western blot / VPA Female NPC
(DIV7, VPA 0.3 mM) / NPCs were cultured from 10 female embryos pooled together from each litter. / Four litters (n = 4)
4A / Western blot / Con Male NPC
(DIV7) / NPCs were cultured from 10 male embryos pooled together from each litter. / Four litters (n = 4)
4A / Western blot / Con Female NPC
(DIV7) / NPCs were cultured from 10 female embryos pooled together from each litter. / Four litters (n = 4)
4A / Western blot / VPA Male NPC
(DIV7, VPA 0.2 mM) / NPCs were cultured from 10 male embryos pooled together from each litter. / Four litters (n = 4)
4A / Western blot / VPA Female NPC
(DIV7, VPA 0.2 mM) / NPCs were cultured from 10 female embryos pooled together from each litter. / Four litters (n = 4)
4B / Western blot / Con Male NPC
(DIV7) / NPCs were cultured from 10 male embryos pooled together from each litter. / Four litters (n = 4)
4B / Western blot / Con Female NPC
(DIV7) / NPCs were cultured from 10 female embryos pooled together from each litter. / Four litters (n = 4)
4B / Western blot / VPA Male NPC
(DIV7, VPA 0.5 mM) / NPCs were cultured from 10 male embryos pooled together from each litter. / Four litters (n = 4)
4B / Western blot / VPA Female NPC
(DIV7, VPA 0.5 mM) / NPCs were cultured from 10 female embryos pooled together from each litter. / Four litters (n = 4)

(3)

Figure / Method / Group / Embryo/Pup / Pregnant mothers
5 / Western blot / Con / NPCs were cultured from 10 male embryos pooled together from each litter. / Three litters (n = 3)
5 / Western blot / VPA / NPCs were cultured from 10 male embryos pooled together from each litter. / Three litters (n = 3)
5 / Western blot / VPM / NPCs were cultured from 10 male embryos pooled together from each litter. / Three litters (n = 3)
5 / Western blot / TSA / NPCs were cultured from 10 male embryos pooled together from each litter. / Three litters (n = 3)
5 / Western blot / SB / NPCs were cultured from 10 male embryos pooled together from each litter. / Three litters (n = 3)
5 / Western blot / LiCl / NPCs were cultured from 10 male embryos pooled together from each litter. / Three litters (n = 3)
5 / Western blot / TDZD / NPCs were cultured from 10 male embryos pooled together from each litter. / Three litters (n = 3)
6A / Western blot / Male Lipo / NPCs were cultured from 10 male embryos pooled together from each litter. / Three litters (n = 3)
6A / Western blot / Male Con siRNA
(50 pmol) / NPCs were cultured from 10 male embryos pooled together from each litter. / Three litters (n = 3)
6A / Western blot / Male MeCP2 siRNA
(50 pmol) / NPCs were cultured from 10 male embryos pooled together from each litter. / Three litters (n = 3)
6A / Western blot / Female Lipo / NPCs were cultured from 10 female embryos pooled together from each litter. / Three litters (n = 3)
6A / Western blot / Female Con siRNA
(50 pmol) / NPCs were cultured from 10 female embryos pooled together from each litter. / Three litters (n = 3)
6A / Western blot / Female MeCP2 siRNA
(50 pmol) / NPCs were cultured from 10 female embryos pooled together from each litter. / Three litters (n = 3)
6B / Western blot / Male Lipo / NPCs were cultured from 10 male embryos pooled together from each litter. / Three litters (n = 3)
6B / Western blot / Male Con siRNA
(50 pmol) / NPCs were cultured from 10 male embryos pooled together from each litter. / Three litters (n = 3)
6B / Western blot / Male MeCP2 siRNA
(50 pmol) / NPCs were cultured from 10 male embryos pooled together from each litter. / Three litters (n = 3)
6B / Western blot / Female Lipo / NPCs were cultured from 10 female embryos pooled together from each litter. / Three litters (n = 3)
6B / Western blot / Female Con siRNA
(50 pmol) / NPCs were cultured from 10 female embryos pooled together from each litter. / Three litters (n = 3)
6B / Western blot / Female MeCP2 siRNA
(50 pmol) / NPCs were cultured from 10 female embryos pooled together from each litter. / Three litters (n = 3)

(4)

Figure / Method / Group / Embryo/Pup / Pregnant mothers
7 / Western blot / Male Lipo / NPCs were cultured from 10 male embryos pooled together from each litter. / Four litters (n = 4)
7 / Western blot / Male Con siRNA
(100 pmol) / NPCs were cultured from 10 male embryos pooled together from each litter. / Four litters (n = 4)
7 / Western blot / Male MeCP2 siRNA
(100 pmol) / NPCs were cultured from 10 male embryos pooled together from each litter. / Four litters (n = 4)
7 / Western blot / Female Lipo / NPCs were cultured from 10 female embryos pooled together from each litter. / Four litters (n = 4)
7 / Western blot / Female Con siRNA
(100 pmol) / NPCs were cultured from 10 female embryos pooled together from each litter. / Four litters (n = 4)
7 / Western blot / Female MeCP2 siRNA
(100 pmol) / NPCs were cultured from 10 female embryos pooled together from each litter. / Four litters (n = 4)