Supplemental Table 1: Results of expression analysis of marker genes

das / HvPP2A / HvACTIN / HvCEREBA / HvBM9
5 / 22,79 (±0,95) / 22,30 (±0,35) / n.d. / 35,38 (±0,21)
7 / 22,81 (±1,08) / 22,34 (±1,13) / n.d. / 36,55 (±1,36)
9 / 22,84 (±0,76) / 21,77 (±0,77) / n.d. / 36,73 (±0,54)
11 / 22,48 (±0,83) / 21,24 (±0,69) / n.d. / 36,74 (±0,74)
13 / 22,29 (±1,14) / 21,35 (±0,99) / n.d. / 36,72 (±1,19)
15 / 22,22 (±1,15) / 21,28 (±1,02) / n.d. / 37,21 (±0,52)
17 / 22,18 (±1,04) / 21,01 (±1,10) / n.d. / 36,79 (±1,08)
19 / 22,22 (±0,80) / 21,29 (±0,56) / n.d. / 36,59 (±1,29)
21 / 22,36 (±1,25) / 21,25 (±0,94) / n.d. / 36,49 (±1,61)
23 / 21,93 (±0,83) / 21,13 (±0,73) / n.d. / 36,74(±1,33)
25 / 22,03 (±0,74) / 20,86 (±0,55) / n.d. / 36,35(±1,21)
27 / 21,87 (±0,75) / 20,39 (±0,68) / n.d. / 36,55(±0,79)
29 / 21,94 (±0,65) / 20,45 (±0,72) / n.d. / 36,40(±1,20)
31 / 21,81(±0,61) / 20,44(±0,79) / n.d. / 36,56(±0,79)
33 / 22,08(±0,81) / 20,78(±1,38) / n.d. / 36,73(±1,48)
35 / 22,29(±0,86) / 20,46(±0,41) / n.d. / 36,23(±1,08)
37 / 22,75(±0,06) / 20,91(±0,58) / n.d. / n.d.

Supplemental Table 1 Average and standard deviation of CT values from qRT-PCR of constitutively expressed marker genes HvPP2A and HvACTIN as well as not expressed retrotransposon HvCEREBA and the MADS box gene HvBM9 of at least four biological replicates. For every gene several primer pairs were tested and all showed the same results. Genes showing CT values over 35 are considered as not expressed. das: days after sowing; CT: cycle threshold; n.d.: not detectable (CT value over 40).