Supplementary Table 1. Morphologicalanalysis of DMSO (control) and NRX195183 + NRX204723 (1:2)-treated AML1-ETO-expressing BM cells as assessed by differential counts after 2, 4 and 6 weeks of treatment in in vitro culture
Cytospin % / D21 of colony assay2 weeks in culture with drugs / D35 of colony assay
4 weeks in culture with drugs / D49 of colony assay
6 weeks in culture with drugs
DMSO (n=4) / NRX195183 + NRX204723 (1:2) (n=4) / DMSO (n=4) / NRX195183 + NRX204723 (1:2) (n=4) / DMSO (n=4) / NRX195183 + NRX204723 (1:2) (n=4)
Blasts / 72.0 3.2 / 73.0 5.0 / 54.8 5.4* / 77.0 7.2* / 43.5 13.5* / 89.8 3.6*
Intermediate / 23.0 2.9 / 20.3 3.8 / 30.0 6.2 / 16.8 3.8 / 48.8 13.7* / 9.5 3.5*
Neutrophils / 3.0 1.4 / 3.3 1.1 / 6.8 4.2 / 5.8 4.1 / 4.5 2.1 / 0.8 0.3
Macrophages / 2.0 0.9 / 3.5 1.5 / 8.5 3.0† / 0.5 0.3† / 3.3 1.6 / 0.0 0.0
Data expressed as Mean ± SEM, *p<0.05, †p=0.05-0.10 by Student’s paired t test
Supplementary Table 2. Morphologicalanalysis of DMSO (control) and NRX195183 + NRX204723 (1:5)-treated AML1-ETO-expressing BM cells as assessed by differential counts after 2, 4 and 6 weeks of treatment in in vitro culture
Cytospin % / D21 of colony assay2 weeks in culture with drugs / D35 of colony assay
4 weeks in culture with drugs / D49 of colony assay
6 weeks in culture with drugs
DMSO (n=4) / NRX195183 + NRX204723 (1:5) (n=3) / DMSO (n=4) / NRX195183 + NRX204723 (1:5) (n=4) / DMSO (n=4) / NRX195183 + NRX204723 (1:5) (n=4)
Blasts / 72.0 3.2 / 74.3 1.5 / 54.8 5.4 / 68.8 9.5 / 43.5 13.5* / 83.8 4.8*
Intermediate / 23.0 2.9 / 18.0 1.0 / 30.0 6.2 / 22.8 6.7 / 48.8 13.7† / 12.3 2.9†
Neutrophils / 3.0 1.4 / 4.3 0.7 / 6.8 4.2 / 8.0 2.9 / 4.5 2.1 / 4.0 2.0
Macrophages / 2.0 0.9* / 3.3 1.5* / 8.5 3.0† / 0.5 0.3† / 3.3 1.6 / 0.0 0.0
Data expressed as Mean ± SEM, *p<0.05, †p=0.05-0.10 by Student’s paired t test
Supplementary Table 3. Differentially expressed probe sets between DMSO (control) and ATRA-treated AML1-ETO-expressing BM cells after 8 hours and 24 hours of treatment (No downweighting of data)
(A)Three differentially expressed probe sets between DMSO (control) and ATRA-treated AML1-ETO-expressing BM cells after 8 hours of treatment
Rank / Adjusted p-value / Fold Change / Mouse Gene 1.0 ST Array Probe Set ID / Gene ID1 / 0.023623 / 1.860331856 / 10590957 / Fut4
2 / 0.032309 / 9.39120966 / 10534667 / Serpine1
3 / 0.03499 / 1.890909085 / 10534202 / Ncf1
(B) Eight differentially expressed probe sets between DMSO (control) and ATRA-treated AML1-ETO-expressing BM cells after 24 hours of treatment
Rank / Adjusted p-value / Fold Change / Mouse Gene 1.0 ST Array Probe Set ID / Gene ID1 / 0.024761 / 11.26419258 / 10534667 / Serpine1
2 / 0.038109 / 3.810655003 / 10469046 / Phyh
3 / 0.040392 / 1.720638471 / 10590957 / Fut4
4 / 0.043062 / 1.803403217 / 10534202 / Ncf1
5 / 0.043062 / 3.117989349 / 10512156 / Aqp3
6 / 0.043062 / 2.396520033 / 10575873 / Osgin1
7 / 0.043062 / 13.12395821 / 10462853 / Cyp26a1
8 / 0.043062 / 2.482285403 / 10384572 / 1110067D22Rik
Supplementary Table 4. Gene sets that were significantly enriched (p<0.05 and FDR<0.25) in the 24 hour ATRA-treated in comparison to the DMSO (control)-treated AML1-ETO-expressing BM cells as generated from the GSEA version 2.0 programme developed by the Broad Institute
NAME OF GENE SET / SUMMARY / NO OF GENES / ES / NES / NOM p-val / FDR / FWERTNFALPHA_TGZ_ADIP_UP / Lipid metabolism / 13 / -0.89663297 / -2.2037644 / 0 / 0.004106847 / 0.004
CPR_NULL_LIVER_UP / Lipid metabolism / 25 / -0.73944736 / -2.133071 / 0 / 0.003078933 / 0.006
ROS_MOUSE_AORTA_DN / Lipid metabolism / 70 / -0.598875 / -2.0698109 / 0 / 0.007240728 / 0.021
FERRARI_4HPR_UP / Cell growth and differentiation / 21 / -0.7183006 / -1.9856783 / 0 / 0.02650077 / 0.097
HSA00480_GLUTATHIONE_METABOLISM / Glutathione/ROS metabolism / 35 / -0.6384755 / -1.9529383 / 0.00149925 / 0.035141 / 0.153
VERNELL_PRB_CLSTR2 / Cell growth and proliferation / 15 / -0.77947414 / -1.9489732 / 0 / 0.03187248 / 0.166
VERHAAK_AML_NPM1_MUT_VS_WT_UP / Cell growth and differentiation / 159 / -0.5041719 / -1.9426689 / 0 / 0.029985504 / 0.181
INSULIN_ADIP_SENS_UP / Lipid metabolism / 11 / -0.8284898 / -1.9387981 / 0 / 0.028958319 / 0.198
EICOSANOID_SYNTHESIS / Lipid metabolism / 15 / -0.7568821 / -1.9095455 / 0.001631321 / 0.040283795 / 0.292
HDACI_COLON_SUL2HRS_UP / Cell growth and proliferation / 10 / -0.84908915 / -1.8968189 / 0 / 0.04476478 / 0.349
HDACI_COLON_CUR2HRS_UP / Cell growth and proliferation / 24 / -0.678061 / -1.8850182 / 0 / 0.04748107 / 0.389
GLUTATHIONE_METABOLISM / Glutathione/ROS metabolism / 29 / -0.6350096 / -1.8840866 / 0.001470588 / 0.044381093 / 0.394
GALINDO_ACT_UP / Inflammatory response and apoptosis / 82 / -0.52957326 / -1.8462719 / 0 / 0.072774015 / 0.588
RUTELLA_HEPATGFSNDCS_UP / Cell growth and differentiation / 137 / -0.4877525 / -1.8412852 / 0 / 0.071881644 / 0.61
LAL_KO_3MO_UP / Lipid metabolism / 46 / -0.57113093 / -1.8327395 / 0.001426534 / 0.07541125 / 0.66
HIF1_TARGETS / Hypoxia/Stress / 32 / -0.61377174 / -1.8273976 / 0.00143472 / 0.07555772 / 0.691
ELECTRON_TRANSPORTER_ACTIVITY / Other / 92 / -0.5041298 / -1.8100375 / 0.001256281 / 0.08932879 / 0.772
JNK_DN / Inflammatory response and immunity / 31 / -0.5977251 / -1.80781 / 0 / 0.08592054 / 0.775
LEE_ACOX1_DN / Other / 57 / -0.5330798 / -1.7998605 / 0.001371742 / 0.08893612 / 0.803
ADIP_DIFF_UP / Lipid metabolism / 66 / -0.5284093 / -1.7984349 / 0.001347709 / 0.086199254 / 0.809
ABBUD_LIF_UP / Cell growth and proliferation / 43 / -0.56216335 / -1.7979674 / 0.001428571 / 0.08254185 / 0.814
HSA00590_ARACHIDONIC_ACID_METABOLISM / Lipid metabolism / 39 / -0.5702912 / -1.7967379 / 0.001488095 / 0.079878576 / 0.819
ZMPSTE24_KO_UP / Cell growth and proliferation / 29 / -0.62245405 / -1.793928 / 0.001488095 / 0.07929722 / 0.83
HYPOXIA_REG_UP / Hypoxia/Stress / 31 / -0.6034569 / -1.7880125 / 0.001459854 / 0.08236583 / 0.846
HALMOS_CEBP_UP / Cell differentiation / 43 / -0.5682088 / -1.7768291 / 0.002902758 / 0.09149259 / 0.884
BASSO_GERMINAL_CENTER_CD40_DN / Inflammatory response and immunity / 62 / -0.522189 / -1.7701991 / 0 / 0.09574378 / 0.908
UVB_NHEK3_C6 / Other / 26 / -0.62978035 / -1.7669494 / 0.00608828 / 0.09593368 / 0.914
ADIPOGENESIS_HMSC_CLASS8_DN / Lipid metabolism / 29 / -0.58969223 / -1.7659153 / 0.003053435 / 0.09376932 / 0.919
HSA00980_METABOLISM_OF_XENOBIOTICS_BY_CYTOCHROME_P450 / Lipid metabolism / 35 / -0.5700272 / -1.7546513 / 0.001455604 / 0.1032829 / 0.941
IDX_TSA_UP_CLUSTER1 / Lipid metabolism / 23 / -0.62374735 / -1.7513766 / 0.00309119 / 0.1030935 / 0.946
GO_ROS / ROS metabolism / 27 / -0.5906672 / -1.7508097 / 0.002936858 / 0.10044012 / 0.948
STURLA_SONIC_HEDGEHOG / Cell growth and proliferation / 15 / -0.6903372 / -1.7356391 / 0.003327787 / 0.11564308 / 0.971
YANG_OSTECLASTS_SIG / Other / 38 / -0.551242 / -1.7214606 / 0.00143472 / 0.1311802 / 0.983
STATIN_PATHWAY_PHARMGKB / Lipid metabolism / 16 / -0.6900307 / -1.7209092 / 0 / 0.12787263 / 0.983
LEE_MYC_UP / Cell growth and differentiation / 50 / -0.5249082 / -1.7013066 / 0 / 0.15489082 / 0.994
H2O2_CSBRESCUED_UP / Other / 50 / -0.51789284 / -1.6993806 / 0.001377411 / 0.15318291 / 0.994
HYPOXIA_FIBRO_UP / Hypoxia/ Stress / 17 / -0.6584013 / -1.6981715 / 0.008025683 / 0.15070279 / 0.995
HINATA_NFKB_UP / Inflammatory response and apoptosis / 99 / -0.46528032 / -1.6949966 / 0 / 0.1515379 / 0.996
TSA_CD4_DN / Cell growth and differentiation / 18 / -0.6366945 / -1.6925906 / 0.00949367 / 0.15175097 / 0.997
ZMPSTE24_KO_DN / Cell growth and proliferation / 24 / -0.60548705 / -1.6899776 / 0.015060241 / 0.1526918 / 0.997
CISPLATIN_PROBCELL_UP / Cell growth and proliferation / 16 / -0.63063073 / -1.6590889 / 0.01300813 / 0.20380378 / 1
IFNA_HCMV_6HRS_UP / Inflammatory response and immunity / 40 / -0.5261046 / -1.6570226 / 0.002886003 / 0.20283371 / 1
CMV_ALL_DN / Other / 94 / -0.45773703 / -1.6517315 / 0.002544529 / 0.20911899 / 1
HSIAO_LIVER_SPECIFIC_GENES / Other / 207 / -0.4206686 / -1.6444552 / 0 / 0.22149062 / 1
LAL_KO_6MO_UP / Lipid metabolism / 61 / -0.4881719 / -1.6422596 / 0.005405406 / 0.22146052 / 1
CHESLER_BRAIN_HIGHEST_VARIANCE_GENES / Other / 34 / -0.53389734 / -1.6351354 / 0.012534819 / 0.23143302 / 1
INOS_ALL_DN / Inflammatory response and immunity / 73 / -0.46333024 / -1.6349403 / 0.00520156 / 0.22715463 / 1
HSA00591_LINOLEIC_ACID_METABOLISM / Lipid metabolism / 22 / -0.5782358 / -1.6275456 / 0.013245033 / 0.23897777 / 1
PRMT5_KD_DN / Cell growth and proliferation / 24 / -0.57718104 / -1.625905 / 0.014684288 / 0.23775163 / 1
HOFMANN_MDS_CD34_LOW_AND_HIGH_RISK / Cell growth and differentiation / 39 / -0.519768 / -1.6244726 / 0.007132668 / 0.23652145 / 1
CARIES_PULP_HIGH_UP / Other / 70 / -0.46878275 / -1.6195192 / 0.003947368 / 0.24299388 / 1
P53HYPOXIAPATHWAY / Hypoxia/ Stress / 17 / -0.61455053 / -1.6159905 / 0.021052632 / 0.24622431 / 1
WIELAND_HEPATITIS_B_INDUCED / Inflammatory response and immunity / 78 / -0.46343175 / -1.6146802 / 0.005161291 / 0.2445307 / 1
HSA00860_PORPHYRIN_AND_CHLOROPHYLL_METABOLISM / Other / 26 / -0.57082915 / -1.6144054 / 0.014925373 / 0.24058163 / 1
HSA00720_REDUCTIVE_CARBOXYLATE_CYCLE / Other / 10 / -0.7037612 / -1.603312 / 0.019900497 / 0.2493061 / 1
CMV_HCMV_TIMECOURSE_24HRS_DN / Inflammatory response and immunity / 37 / -0.5216943 / -1.602108 / 0.014025246 / 0.24793233 / 1
Abbreviations:
ESenrichment score
NESnormalized enrichment score
NOM p-valnominal p value
FDRfalse discovery rate
FWERfamily wise error rate
Supplementary Table 5. Gene sets that were significantly enriched (p<0.05 and FDR<0.25) in the 3 week NRX195183-treated in comparison to the DMSO (control)-treated AML1-ETO-expressing BM cells as generated from the GSEA version 2.0 programme developed by the Broad Institute
NAME / SUMMARY / NO OF GENES / ES / NES / NOM p-val / FDR / FWERHOFFMANN_LARGE_TO_SMALL_PRE_BII_LYMPHOCYTE_UP / Cell differentiation / 98 / -0.7657594 / -2.721069 / 0 / 0 / 0
MORI_LARGE_PRE_BII_LYMPHOCYTE_UP / Cell growth and proliferation / 55 / -0.8120854 / -2.638434 / 0 / 0 / 0
MARKEY_RB1_ACUTE_LOF_DN / Inflammatory response and immunity / 232 / -0.644336 / -2.567897 / 0 / 0 / 0
MORI_IMMATURE_B_LYMPHOCYTE_DN / Cell differentiation / 54 / -0.8141823 / -2.546909 / 0 / 0 / 0
IVANOVA_HEMATOPOIESIS_MATURE_CELL / Cell differentiation / 120 / -0.6679797 / -2.447336 / 0 / 0 / 0
LE_EGR2_TARGETS_UP / Other / 108 / -0.6717640 / -2.426993 / 0 / 0 / 0
YU_MYC_TARGETS_UP / Cell growth and proliferation / 40 / -0.8086124 / -2.423082 / 0 / 0 / 0
BOYLAN_MULTIPLE_MYELOMA_PCA1_UP / Cell differentiation / 112 / -0.6543673 / -2.422814 / 0 / 0 / 0
LIAN_LIPA_TARGETS_6M / Lipid metabolism / 86 / -0.6892836 / -2.410796 / 0 / 0 / 0
ICHIBA_GRAFT_VERSUS_HOST_DISEASE_35D_UP / Inflammatory response and immunity / 151 / -0.644025 / -2.408772 / 0 / 0 / 0
LIAN_LIPA_TARGETS_3M / Lipid metabolism / 70 / -0.6897874 / -2.332897 / 0 / 0 / 0
ICHIBA_GRAFT_VERSUS_HOST_DISEASE_D7_UP / Inflammatory response and immunity / 127 / -0.5741358 / -2.119219 / 0 / 1.38E-04 / 0.001
STEARMAN_TUMOR_FIELD_EFFECT_UP / Inflammatory response and immunity / 40 / -0.6893492 / -2.108217 / 0 / 1.27E-04 / 0.001
MARKEY_RB1_ACUTE_LOF_UP / Cell growth and proliferation / 244 / -0.5225912 / -2.071842 / 0 / 2.31E-04 / 0.002
IVANOVA_HEMATOPOIESIS_LATE_PROGENITOR / Cell differentiation / 143 / -0.5489067 / -2.069028 / 0 / 2.16E-04 / 0.002
GOLDRATH_ANTIGEN_RESPONSE / Cell proliferation and differentiation / 368 / -0.4930817 / -2.065050 / 0 / 2.02E-04 / 0.002
BOYLAN_MULTIPLE_MYELOMA_C_D_DN / Inflammatory response and immunity; cell adhesion / 285 / -0.5026038 / -2.052790 / 0 / 2.90E-04 / 0.003
MORI_SMALL_PRE_BII_LYMPHOCYTE_DN / Cell differentiation / 66 / -0.6021485 / -2.029261 / 0 / 3.75E-04 / 0.004
STEARMAN_LUNG_CANCER_EARLY_VS_LATE_DN / Other / 63 / -0.5927867 / -1.972913 / 0 / 0.001245861 / 0.014
YAO_TEMPORAL_RESPONSE_TO_PROGESTERONE_CLUSTER_15 / Cell proliferation and immunity / 35 / -0.6755705 / -1.966849 / 0 / 0.001183568 / 0.014
CAIRO_LIVER_DEVELOPMENT_DN / Cell proliferation and differentiation / 222 / -0.4990573 / -1.960532 / 0 / 0.001127208 / 0.014
PAL_PRMT5_TARGETS_UP / Cell growth and proliferation / 198 / -0.4959442 / -1.958969 / 0 / 0.001075971 / 0.014
MORI_MATURE_B_LYMPHOCYTE_DN / Cell differentiation / 57 / -0.5933388 / -1.941380 / 0 / 0.00163699 / 0.022
MORI_EMU_MYC_LYMPHOMA_BY_ONSET_TIME_UP / Cell growth and proliferation / 106 / -0.5472631 / -1.939047 / 0 / 0.00163851 / 0.023
LEE_LIVER_CANCER_DENA_UP / Cell growth and proliferation; anti-apoptosis / 56 / -0.5896419 / -1.897649 / 0 / 0.002924392 / 0.04
KENNY_CTNNB1_TARGETS_UP / Cell proliferation and differentiation / 43 / -0.6011388 / -1.849524 / 0 / 0.005463495 / 0.079
LEE_LIVER_CANCER_MYC_UP / Cell growth and proliferation / 50 / -0.581378 / -1.848411 / 0 / 0.005261143 / 0.079
LEE_LIVER_CANCER_MYC_TGFA_DN / Liver metabolism (Other) / 56 / -0.5665790 / -1.835480 / 0 / 0.006512498 / 0.103
COATES_MACROPHAGE_M1_VS_M2_DN / Inflammatory response and immunity / 81 / -0.5285608 / -1.835286 / 0 / 0.006287929 / 0.103
BOYLAN_MULTIPLE_MYELOMA_C_CLUSTER_UP / Cell growth and proliferation; metastasis / 36 / -0.6338332 / -1.827757 / 0.004587156 / 0.006751097 / 0.112
TSAI_RESPONSE_TO_RADIATION_THERAPY / Inflammatory response and immunity / 29 / -0.6359664 / -1.823594 / 0.006479482 / 0.006822742 / 0.117
HESS_TARGETS_OF_HOXA9_AND_MEIS1_DN / Cell differentiation / 88 / -0.5129475 / -1.796357 / 0.002617801 / 0.00875388 / 0.151
BOYLAN_MULTIPLE_MYELOMA_C_UP / Cell growth and proliferation; metastasis / 31 / -0.6317121 / -1.791977 / 0 / 0.009195051 / 0.161
RIZ_ERYTHROID_DIFFERENTIATION / Cell differentiation / 79 / -0.5198590 / -1.780733 / 0 / 0.010293735 / 0.185
LEE_LIVER_CANCER_CIPROFIBRATE_DN / Cell growth and proliferation / 58 / -0.5394583 / -1.755572 / 0 / 0.012977611 / 0.24
PAL_PRMT5_TARGETS_DN / Cell growth and proliferation / 32 / -0.6168823 / -1.745439 / 0.002242153 / 0.014483123 / 0.266
LIANG_HEMATOPOIESIS_STEM_CELL_NUMBER_LARGE_VS_TINY_UP / Stem cell regulation / 42 / -0.5720665 / -1.730600 / 0.004291846 / 0.01686499 / 0.309
LEE_LIVER_CANCER_CIPROFIBRATE_UP / Cell proliferation and lipid metabolism / 56 / -0.5324914 / -1.725938 / 0.002347418 / 0.017132757 / 0.319
LEE_LIVER_CANCER_E2F1_DN / Cell growth and proliferation; anti-apoptosis / 53 / -0.5391613 / -1.722860 / 0 / 0.01711492 / 0.325
BERENJENO_TRANSFORMED_BY_RHOA_FOREVER_DN / Cell proliferation and differentiation / 32 / -0.5950635 / -1.717285 / 0.004405286 / 0.017747194 / 0.342
CHESLER_BRAIN_HIGHEST_GENETIC_VARIANCE / Other / 40 / -0.5685008 / -1.704208 / 0.002415459 / 0.019344507 / 0.376
HOFFMANN_SMALL_PRE_BII_TO_IMMATURE_B_LYMPHOCYTE_UP / Cell differentiation / 43 / -0.5445054 / -1.703270 / 0.014051522 / 0.019244269 / 0.379
BERENJENO_TRANSFORMED_BY_RHOA_FOREVER_UP / Cell growth and proliferation / 23 / -0.6367403 / -1.699074 / 0.009090909 / 0.019620104 / 0.391
CADWELL_ATG16L1_TARGETS_UP / Lipid metabolism; inflammatory response / 104 / -0.4818939 / -1.694478 / 0 / 0.020309877 / 0.408
BOYLAN_MULTIPLE_MYELOMA_D_DN / Cell proliferation / 82 / -0.4909462 / -1.691794 / 0 / 0.02039133 / 0.416
MCBRYAN_PUBERTAL_BREAST_3_4WK_UP / Cell proliferation and differentiation / 219 / -0.4245987 / -1.689397 / 0 / 0.02053706 / 0.423
MCBRYAN_PUBERTAL_BREAST_6_7WK_DN / Cell proliferation and differentiation / 86 / -0.4838572 / -1.683240 / 0 / 0.020956162 / 0.435
YAO_TEMPORAL_RESPONSE_TO_PROGESTERONE_CLUSTER_8 / Cell proliferation and immunity / 49 / -0.5256445 / -1.675845 / 0.014084507 / 0.021893257 / 0.458
LEE_LIVER_CANCER_DENA_DN / Liver metabolism (Other) / 65 / -0.50764 / -1.666282 / 0 / 0.023369856 / 0.484
KHETCHOUMIAN_TRIM24_TARGETS_UP / Cell growth and proliferation / 47 / -0.5338227 / -1.660925 / 0.009302326 / 0.023943562 / 0.497
SWEET_LUNG_CANCER_KRAS_DN / Cell growth and proliferation / 446 / -0.3894042 / -1.651489 / 0 / 0.02497006 / 0.523
ZHANG_BREAST_CANCER_PROGENITORS_UP / Cell growth and proliferation / 415 / -0.3878733 / -1.624328 / 0 / 0.030304946 / 0.594
MCBRYAN_PUBERTAL_BREAST_4_5WK_UP / Cell proliferation and differentiation / 273 / -0.4053507 / -1.620994 / 0 / 0.03072342 / 0.611
HESS_TARGETS_OF_HOXA9_AND_MEIS1_UP / Cell growth and proliferation / 64 / -0.4862404 / -1.614854 / 0.004889976 / 0.03198004 / 0.63
HOFFMANN_IMMATURE_TO_MATURE_B_LYMPHOCYTE_DN / Cell differentiation / 30 / -0.5557698 / -1.612233 / 0.013636364 / 0.03196429 / 0.637
LEE_LIVER_CANCER_ACOX1_DN / Cell proliferation and lipid metabolism / 57 / -0.4965660 / -1.611426 / 0.016241299 / 0.03160467 / 0.639
FOSTER_INFLAMMATORY_RESPONSE_LPS_UP / Inflammatory response and immunity / 179 / -0.4099944 / -1.597082 / 0 / 0.035228893 / 0.684
MARKEY_RB1_CHRONIC_LOF_UP / Cell growth and proliferation / 114 / -0.4431312 / -1.595338 / 0.002631579 / 0.035137627 / 0.687
LEE_TARGETS_OF_PTCH1_AND_SUFU_DN / Cell growth and proliferation / 58 / -0.4967002 / -1.584864 / 0.01 / 0.037761696 / 0.721
SEKI_INFLAMMATORY_RESPONSE_LPS_UP / Inflammatory response and immunity / 81 / -0.4642023 / -1.580165 / 0.005037783 / 0.038246214 / 0.731
COATES_MACROPHAGE_M1_VS_M2_UP / Inflammatory response and immunity / 89 / -0.4435695 / -1.553800 / 0.014778325 / 0.046854988 / 0.799
HOFFMANN_SMALL_PRE_BII_TO_IMMATURE_B_LYMPHOCYTE_DN / Cell differentiation / 23 / -0.5771046 / -1.528977 / 0.041763343 / 0.05565922 / 0.865
MARTORIATI_MDM4_TARGETS_NEUROEPITHELIUM_DN / Apoptosis / 85 / -0.4357150 / -1.520266 / 0.012195122 / 0.058343705 / 0.881
LEE_LIVER_CANCER_MYC_E2F1_DN / Cell growth and proliferation; anti-apoptosis / 56 / -0.4598945 / -1.496710 / 0.020134227 / 0.06853874 / 0.92
RIZ_ERYTHROID_DIFFERENTIATION_APOBEC2 / Cell differentiation / 27 / -0.5317883 / -1.492192 / 0.046296295 / 0.06975372 / 0.927
TSENG_ADIPOGENIC_POTENTIAL_DN / Cell differentiation and lipid metabolism / 47 / -0.4735147 / -1.481092 / 0.041062802 / 0.07432049 / 0.943
BERENJENO_TRANSFORMED_BY_RHOA_REVERSIBLY_DN / Cell proliferation and differentiation / 29 / -0.5103375 / -1.4772980 / 0.041763343 / 0.07498118 / 0.946
MONNIER_POSTRADIATION_TUMOR_ESCAPE_UP / Hypoxia/Stress and cell migration / 388 / -0.3494943 / -1.471200 / 0 / 0.07720152 / 0.951
LEE_LIVER_CANCER_E2F1_UP / Cell growth and proliferation; anti-apoptosis / 56 / -0.4501294 / -1.464002 / 0.033573143 / 0.080620855 / 0.961
YAO_HOXA10_TARGETS_VIA_PROGESTERONE_UP / Cell proliferation and immunity / 83 / -0.4225099 / -1.461068 / 0.02173913 / 0.08090073 / 0.962
GERY_CEBP_TARGETS / Cell growth and differentiation / 136 / -0.3978118 / -1.460701 / 0.01 / 0.08008832 / 0.964
VARELA_ZMPSTE24_TARGETS_UP / Stress/Cellular senescence / 42 / -0.4686644 / -1.460123 / 0.045346063 / 0.07926277 / 0.964
RIZ_ERYTHROID_DIFFERENTIATION_CCNE1 / Cell differentiation / 42 / -0.4780327 / -1.457912 / 0.044392522 / 0.07959295 / 0.966
YU_MYC_TARGETS_DN / Inflammatory response and immunity; apoptosis / 58 / -0.4571111 / -1.454961 / 0.039443154 / 0.079949014 / 0.968
RAMALHO_STEMNESS_DN / Stem cell regulation / 77 / -0.4174964 / -1.444809 / 0.024752475 / 0.08461268 / 0.977
MARKEY_RB1_CHRONIC_LOF_DN / Inflammatory response and immunity / 128 / -0.3985847 / -1.438118 / 0.030690538 / 0.08792877 / 0.98
RASHI_RESPONSE_TO_IONIZING_RADIATION_2 / Inflammatory response and immunity; apoptosis / 128 / -0.3889478 / -1.434814 / 0.010282776 / 0.08658014 / 0.982
CADWELL_ATG16L1_TARGETS_DN / Inflammatory response;lipid metabolism / 69 / -0.4198042 / -1.422795 / 0.032258064 / 0.09336475 / 0.987
RASHI_RESPONSE_TO_IONIZING_RADIATION_4 / Inflammatory response and immunity / 62 / -0.4306206 / -1.413575 / 0.039906103 / 0.09809917 / 0.99
BYSTRYKH_HEMATOPOIESIS_STEM_CELL_QTL_CIS / Stem cell regulation / 140 / -0.3778125 / -1.393778 / 0.01344086 / 0.10818569 / 0.994
FOSTER_INFLAMMATORY_RESPONSE_LPS_DN / Inflammatory response and immunity / 406 / -0.3236893 / -1.356109 / 0.003246753 / 0.1316494 / 0.999
KUMAR_TARGETS_OF_MLL_AF9_FUSION / Cell growth and proliferation / 431 / -0.3115802 / -1.33014 / 0.006230529 / 0.14457797 / 1
TSENG_IRS1_TARGETS_DN / Cell differentiation and lipid metabolism / 143 / -0.3507875 / -1.321624 / 0.040816326 / 0.14925753 / 1
PASQUALUCCI_LYMPHOMA_BY_GC_STAGE_UP / Cell growth and proliferation / 279 / -0.3118494 / -1.267693 / 0.032934133 / 0.18401489 / 1
MCBRYAN_PUBERTAL_BREAST_4_5WK_DN / Cell proliferation and differentiation / 200 / -0.3196662 / -1.256161 / 0.046376813 / 0.1947966 / 1
MARTORIATI_MDM4_TARGETS_FETAL_LIVER_DN / Apoptosis / 283 / -0.2993306 / -1.219153 / 0.046783626 / 0.22601603 / 1
Abbreviations:
ESenrichment score
NESnormalized enrichment score
NOM p-valnominal p value
FDRfalse discovery rate
FWERfamily wise error rate