Supplemental Table 1 Characterization of 297 novel microsatellite markers genotyped in the mapping family
Locus Name / Primer Sequence(5'-3') / Repeat Motifs / ExpectedSize(bp) / Tm(℃) / GenBank
AccessionNo.
Hdi44 / F:GCTGAGTAAGCAGTGCTGTGG / R:CGTTGACTTCAAGCAGGTTCT / (GT)23 / 184 / 50℃ / HQ650299
Hdi414 / F:TGGATTCGTTGAGATACCAGG / R:TTCACTGCAGCTTGTTGAGC / (AC)33 / 222 / 51℃ / HQ650346
Hdi420 / F:CAACACTGGCTGTCTGATTGA / R:tgctagcagaagcgacagact / (TC)41 / 242 / 51℃ / HQ650267
Hdi3-43 / F:CGTGGTTGTGTAACTCTTAGGC / R:CCATGTGAGGAACTGAACCTG / (TG)27…(GA)7 / 385 / 52℃ / HQ650518
Hdi3-4 / F:GCGGCAGTATCAATGTCTGAG / R:CCTGCAATCACTACCATAACCA / (AGT)7…(GTA)10 / 216 / 52℃ / HQ650532
Hdi3-56 / F:CGTTACAAGCCCCTGTGAGT / R:TTGCACTTTACACCGCTCAG / (AC)38 / 101 / 52℃ / HQ650517
Hdi469 / F:TAAGAAGAAAGGCGAACGGA / R:AAGCAGCCCAGTGGTTAGTG / (AC)12g(CA)8 / 274 / 52℃ / HQ650401
Hdi185 / F:CACCAATTGCTCTGGTTATATTG / R:TTGAGCAGCTAATGCTGGAT / (CACG)8(CA)7…(AC)8g(CA)6(ACAT)6 / 268 / 53℃ / HQ650371
Hdi19 / F:CGAAGAAGACGATCCAGAAGA / R:CTTGcTaGCAGAAGCAGGTGT / (GCA)6…(ACA)8 / 275 / 53℃ / HQ650270
Hdi3-209 / F:CGAATGTGCAGGTGTATCCTT / R:CCAGTCATCCTTGCAATGTAGT / (GT)8 / 391 / 54℃ / HQ650528
Hdi3-212 / F:TGGACCAGATACTCCTGTTGG / R:GTGGTAGAATCGGCTTGTCAT / (AC)31…(AC)7 / 355 / 54℃ / HQ650519
Hdi105 / F:CTACCACCACAAGCACAAGC / R:GTGGTGGTGTTGGTCTTGC / (ACC)6 / 235 / 54℃ / HQ650378
Hdi3-217 / F:ACTTATGTTGGCAATTGCAGG / R:TGTGCTCTGTACCTGATTGGA / (AC)37 / 327 / 55℃ / HQ650439
Hdi125 / F:CGTAGAACAAGTTCCAGGTCG / R:GTCATAGGTGTGTTGTGGATATGAA / (AC)9g(CA)18 / 202 / 55℃ / HQ650269
Hdi126 / F:TCATCTTGGCATTAACACATCC / R:ATGGTTGTATGTGGAGGTGGA / (TC)15(AC)11at(CAC)6 / 234 / 55℃ / HQ650301
Hdi216 / F:TCCAAAAATGCTGTCATCTACA / R:TGCACAAGATGTTATGGCTGA / (CATA)8…(TACA)5(AC)14agag(AC)21(ACAT)9 / 280 / 55℃ / HQ650279
Hdi317 / F:AGACGCAACGTGATACATCCT / R:GCACGAAGAAGAAGTTGGAGG / (CA)11t(AC)23atg(TCTA)5 / 236 / 55℃ / HQ650309
Hdi336 / F:TGAGAACGTTGTCTCACC / R:GGAACAAGGTGAGGATCTGTG / (AG)45 / 205 / 55℃ / HQ650322
Hdi408 / F:CCACAACGTGTGTGTGTAttgA / R:TGtgGCTACTGTTCAGCACAA / (GT)6…(GT)8…(GT)6…(GT)7 / 254 / 55℃ / HQ650367
Hdi3-114 / F:TTGTGTCACTGGATTGTCCTG / R:ATCGACTGCAACAACCACTTC / (TG)19 / 224 / 56℃ / HQ650504
Hdi3-153 / F:TTGAGATGAGCCTGGGAAGT / R:AGGAGATGACACAGGCGAAT / (AC)23 / 276 / 56℃ / HQ650469
Hdi3-155 / F:TTGGATGGAAGCGATACACA / R:CACTCCATCTAGCCAGCCAT / (TGAG)5…(TG)8c(GT)17 / 174 / 56℃ / HQ650467
Hdi3-17 / F:TCACAGTCTTCCTTGGGTCC / R:ACCTCCTCCTCCGTGTCC / (TGG)5 / 164 / 56℃ / HQ650473
Hdi3-184 / F:GCATGTTTGTTAGCGTGTGC / R:GTTTCGTCGTGGGTCAGTTT / (ATGT)24 / 148 / 56℃ / HQ650487
Hdi3-185 / F:AGGTTGCTTGACATGTGGCT / R:GGATGTGGGCGAATAGAGAA / (GT)30 / 278 / 56℃ / HQ650474
Supplemental Table 1 (Continued)
Locus Name / Primer Sequence(5'-3') / Repeat Motifs / ExpectedSize(bp) / Tm(℃) / GenBank
AccessionNo.
Hdi3-187 / F:CAGGAATTAATATGTATGGCCC / R:CTTCCTTCCTCCATGCATTC / (TG)6ta(TG)9…(GT)6…(GT)10…(GT)12 / 277 / 56℃ / HQ650448
Hdi3-213 / F:TGAGCTGACAATTACGTGCC / R:TTCTGGGGACAACAAGATCC / (GT)24 / 267 / 56℃ / HQ650490
Hdi3-215 / F:TGGATGGAGTGTACCAGCAA / R:TGAGCCACAGGCTACAGATG / (GT)18 / 273 / 56℃ / HQ650453
Hdi3-21 / F:CCATTGCGATCAGACACATTA / R:AACATGTCAAGAATTGTGCTTCA / (GT)16gggg(GT)13…(GT)14 / 395 / 56℃ / HQ650502
Hdi3-226 / F:GCGTCCATGAGTATTGCACTT / R:TGCTTCAATGTGTGGACGTTA / (GT)22…(GT)6 / 264 / 56℃ / HQ650533
Hdi3-229 / F:TCAGCAACCATCTCGACAAC / R:CCAACTGTTGCTGTTGCTGT / (ACA)5 / 164 / 56℃ / HQ650523
Hdi3-230 / F:CACCAACATTAAGTAGACTGCGA / R:ACAGGTTGCATGTATTGAATTA / (GT)32 / 187 / 56℃ / HQ650497
Hdi3-31 / F:GCACGTGTGAGAGACGTGTT / R:TCTCACACATCCACCCAAAA / (GT)17 / 155 / 56℃ / HQ650484
Hdi3-33 / F:CGCCAGGCAGGTTAAGAAT / R:ATGTGAGAAAACGAAACCCG / (AC)7(AG)7…(AG)21 / 235 / 56℃ / HQ650472
Hdi3-68 / F:ATGGGGACCGATAAAACACA / R:CATTGTGCACAGGACAGGAC / (ACT)33 / 241 / 56℃ / HQ650512
Hdi3-94 / F:TGATTGCTGTCCCCGTATAA / R:TTTGGGAAATATTCAGTGGGA / (GT)9…(GT)24 / 254 / 56℃ / HQ650479
Hdi115 / F:GGGAGACACTCAACAGGCAT / R:GACAGCGATCTTGACGCATA / (GT)30(GA)9 / 243 / 56℃ / HQ650399
Hdi181 / F:ACGTTTTGTGCGTGTTTTCA / R:GGTGATGGTGATGGTGATGA / (CAC)8(CAT)7…(CAT)6 / 278 / 56℃ / HQ650304
Hdi209 / F:AAGCGTCAAAGGTGAAATGG / R:GCTGGCACTATAAAATCGGC / (GT)17 / 158 / 56℃ / HQ650281
Hdi306 / F:TTGCTAGCAGAAGCCAATCA / R:TTGGTGTTTGTGTCTGAGGTG / (CA)28…(CA)15t(AC)10 / 267 / 56℃ / HQ650394
Hdi313 / F:AAGACCAAGGTTCGTTTCCC / R:CAGATTCTCCTCCTTGCTGG / (AC)24 / 184 / 56℃ / HQ650344
Hdi386 / F:GAAGCGTAGTCGAGGTACACG / R:TCTCAGAGACAGTCATCACTCAG / (GCT)9gagga(TGC)6 / 174 / 56℃ / HQ650244
Hdi40 / F:AACTTGTGCCCAGTCCATTC / R:TCCACTTTGGTATGCAGCAA / (AC)14at(AC)18 / 172 / 56℃ / HQ650286
Hdi432 / F:GCTGAGTGAATGCATGAATGA / R:AAGCAGTTGCTAGTCACATTGAA / (ACAG)6(AC)34 / 213 / 56℃ / HQ650366
Hdi436 / F:ATTCCAGCTATATCACGGCG / R:ATAGGGTAAACTGGGGTGGG / (AC)21 / 245 / 56℃ / HQ650243
Hdi440 / F:TGATGGGAGATGTATGCCCT / R:TTGCATGCATTTCAAGATGA / (AC)7ta(AC)7ta(AC)20 / 189 / 56℃ / HQ650284
Hdi86 / F:CGCGACAACACACGTACAC / R:TTGCTAGCAGAAGCGTCCAT / (CA)6 / 275 / 56℃ / HQ650274
Hdi3-48 / F:ACAAGTCGACGCAGTAACCC / R:GCAAGCAAATCCACTCACAA / (TG)36 / 176 / 57℃ / HQ650463
Hdi3-65 / F:AGGTGTGCGTTTGTAGAGGG / R:TTCTTCTTCACCCCCTCCTT / (GT)9…(TG)20 / 230 / 57℃ / HQ650452
Hdi113 / F:TAGCAGAAGCCTCCGCACT / R:CAATCACTGCATTATCGGACC / (CT)38 / 158 / 57℃ / HQ650332
Supplemental Table 1 (Continued)
Locus Name / Primer Sequence(5'-3') / Repeat Motifs / ExpectedSize(bp) / Tm(℃) / GenBank
AccessionNo.
Hdi120 / F:CATCATGCTGCATAACAACG / R:ACTCGGCCATACTTCACACAC / (GTC)7 / 133 / 57℃ / HQ650266
Hdi121 / F:CAGACAGACAGACACTAGCATGG / R:TTGTGTGTGTATGCAGTGCG / (CA)6 / 123 / 57℃ / HQ650328
Hdi136 / F:CGGCTAAGTGGTAACAACGTC / R:AACTCACTCACTCACTTGGATCAC / (AC)20…(AG)37 / 226 / 57℃ / HQ650242
Hdi138 / F:AGTGTCCTGCCTCTCACTCAG / R:TATGTGAGTGTGTGCGTCTCG / (CA)12a(AC)24 / 205 / 57℃ / HQ650413
Hdi139 / F:TCTATCAGGTACATCTAGCACCTC / R:CTTCACTCATGGTGTGCAGTG / (CA)13…(CA)21cc(CA)7 / 238 / 57℃ / HQ650275
Hdi141 / F:GGACTTCAGGAGATACAAGGTT / R:GCCACAGTCGAGTTGCATT / (CA)32 / 205 / 57℃ / HQ650248
Hdi156 / F:GAGTCACCTAGTAGCCAGCCA / R:GGTCACATATAGGAGTTGATACTAC / (TG)23 / 162 / 57℃ / HQ650381
Hdi161 / F:TCGGTGACACCATACGGTAAT / R:GACATCCAGACCAAGAAGTGC / (GT)17 / 195 / 57℃ / HQ650423
Hdi162 / F:CCAATAATATCAGATGGAGGCTG / R:CATCCACTAACAGGCGACTTG / (GT)31 / 231 / 57℃ / HQ650427
Hdi165 / F:TCGTGACACGTAACACTGCTT / R:ATGATGATCCTTGAGGCTGGT / (CA)32 / 232 / 57℃ / HQ650261
Hdi170 / F:AACCTTAGCAACATCGCAA / R:TGAGTGGTCTCATCCGCTTAC / (GT)24 / 213 / 57℃ / HQ650361
Hdi173 / F:ACTGAAGACAGCCACAGTCGT / R:CCTGTGCAAGACTAATATCCGA / (GT)22t(AG)22 / 246 / 57℃ / HQ650325
Hdi174 / F:CAACACCTGTTCTGTAACACTCAA / R:CCATCTGTGTCGGACATTCTT / (GA)56 / 208 / 57℃ / HQ650273
Hdi175 / F:GGTGAGTGAGTGATCTATTGTGG / R:GGTCTTCGGACTGACGAGTAA / (GA)24gg(GA)32 / 266 / 57℃ / HQ650338
Hdi177 / F:AAGAATCTTCAAGCACAAGCG / R:TGTGAAGATGGAAGCTGATCC / (CAA)6 / 104 / 57℃ / HQ650387
Hdi179 / F:GCTGTGTGTGGACGGTGTT / R:CACGGATACAGACACACAGACATA / (TG)7c(GT)7 / 209 / 57℃ / HQ650386
Hdi180 / F:CCACAGGAGTAGTAGAGGTAGTGG / GTGGAATGTGTACTACGGCCA / (CAG)5(TAG)11,(AC)17 / 203 / 57℃ / HQ650296
Hdi182 / F:ACAGGCAGATATGGACGCTAA / R:caGCCTCTCCATCCAACATAG / (TG)23 / 159 / 57℃ / HQ650307
Hdi192 / F:TGGAATAGAGCTAATGGTTGCAT / R:AAGCGACTAAGAGCAGCTGAG / (CA)20 / 129 / 57℃ / HQ650256
Hdi199 / F:TGCTGAAGGCCAATTCTACC / R:TGAAGGCTATACAGAATATGGCG / (GA)7a(AG)28 / 196 / 57℃ / HQ650415
Hdi205 / F:ACAAGCTCATGATGTTCACCT / R:AATGGCGTTGTGTAATCA / (AC)11 / 171 / 57℃ / HQ650365
Hdi208 / F:TTGTCAAGCAGAATCGTG / R:CGATCTGTATGTGTGCGTGAG / (AC)11 / 174 / 57℃ / HQ650389
Hdi217 / F:cgtagagatgtcgctgtgtca / R:TGTCTGTGCTGGTTGCTGTAG / (AC)28 / 210 / 57℃ / HQ650300
Hdi21 / F:CGAGGTTGTGTTCTTGCTTCA / R:TTGTAAGGCAGTCTATCAACAAGC / (T)10atttt(AC)7ag(AC)7ag(AC)26 / 225 / 57℃ / HQ650407
Hdi221 / F:CAAGGTATTGACCTTACAATGCAC / R:AACAATCCGTTCAATACACC / (CA)29 / 190 / 57℃ / HQ650334
SupplementalTable 1 (Continued)
Locus Name / Primer Sequence(5'-3') / Repeat Motifs / ExpectedSize(bp) / Tm(℃) / GenBank
AccessionNo.
Hdi225 / F:CGAATCACCTTCCTGCCTAC / R:AACAAGGCCTTCTCGTGTA / (AC)25…(ACAT)20 / 338 / 57℃ / HQ650390
Hdi23 / F:CTCTCTGCATTAGAGGCATCG / R:TGGTCATCCTGAACACAGACA / (CAG)10 / 110 / 57℃ / HQ650280
Hdi247 / F:AGGCagcATCAGATCtatcaCA / R:GCATGTTCATCGATGGTTCTT / (CAT)7caa(CAC)7(CAT)6cg(TCA)10 / 183 / 57℃ / HQ650329
Hdi260 / F:TATGACGAGCGAACGTTGTAA / R:AACGTATGCCGTATGACTT / (CT)32 / 183 / 57℃ / HQ650428
Hdi270 / F:CTACTCGACAGGCGATGGTTA / R:CGTTCCTCGTTCATAGGTGAG / (GAT)5(GGT)10 / 213 / 57℃ / HQ650246
Hdi278 / F:ATTGCAAGTTCCTGGACAACA / R:TGCTACCTCTTCACCAACACA / (CA)19 / 177 / 57℃ / HQ650271
Hdi297 / F:TCCTTCGGTATGTATGCATCG / R:ACGCTCTAGCGCCATCAAT / (TC)7a(CT)7(CA)7…(TC)10(AC)6…(TC)7…(CA)9 / 236 / 57℃ / HQ650345
Hdi30 / F:AAGCCGGATAGGTTAGCAGAC / R:AGAATCTGCAGGTTGTGCAT / (TG)32…(GT)8 / 220 / 57℃ / HQ650400
Hdi310 / F:TAATCTGGTGACAGTGATGCG / R:ACTTGCCTTAGCACGTTATGC / (CT)44 / 257 / 57℃ / HQ650373
Hdi311 / F:GAGGAAGTGAGGTGAGACACG / R:CAGAagcttcactcAAGGACG / (GT)14(GA)12gtgt(GA)11 / 239 / 57℃ / HQ650393
Hdi312 / F:AGACACACGTGACCATTCACA / R:ctgagtcctgctccggtc / (CT)32 / 203 / 57℃ / HQ650396
Hdi318 / F:AAGGCGAGCTCTACACTAGGC / R:CAGGAAGTAATCAATGCCTTCAA / (GA)6tc(GA)35 / 217 / 57℃ / HQ650362
Hdi31 / F:TTAAGATACTAATGCGTGCTGG / R:CGTCCACAGGCAGTATCAATAA / (TC)8…(CT)21 / 203 / 57℃ / HQ650412
Hdi326 / F:TAGCAGAAGCACTCTTGCACA / R:GCTGAGAAGAGTGGATGCCTT / (CA)23 / 155 / 57℃ / HQ650419
Hdi327 / F:TACACATGACCATTACCGCTG / R:GTTCGAACCTGCGACCT / (TC)43 / 184 / 57℃ / HQ650417
Hdi329 / F:ATTCACGCAGCTACCAGCTTA / R:TTGAGTGAGTACGTGAGTGCG / (CA)21 / 155 / 57℃ / HQ650257
Hdi338 / F:GCAAGCGGACTATGAACCAT / R:GACGCCATATGGAGACTT / (GC)6(AC)9 / 192 / 57℃ / HQ650403
Hdi347 / F:CATTACACACTCACACAGGCG / R:ATTCTGTTATAGTGCGACGTT / (AC)32 / 196 / 57℃ / HQ650245
Hdi35 / F:GCGTGCACACTCATTATCACA / R:AATTCTGCGGAACACTGGTT / (AC)10,(CA)6…(CA)8 / 293 / 57℃ / HQ650241
Hdi351 / F:ATGATGATCTCCTCGCTGCT / R:ACTCTCACCGTCATCATCACC / (GCT)7 / 178 / 57℃ / HQ650437
Hdi352 / F:TCCGCATCACTACACATCGTA / R:CCTTGCTGACGTTATCGACC / (AG)44 / 164 / 57℃ / HQ650327
Hdi353 / F:CTGAATGACTGCACCATCACA / R:AATGTCAAGCCATCCTTAGCA / (TC)42 / 250 / 57℃ / HQ650438
Hdi354 / F:GAGCGAACGCTCTAACCACTA / R:CAACCGTCACCTTATTGCCTA / (AC)41 / 219 / 57℃ / HQ650276
Hdi356 / F:TGGAGATATATGATGCCTCGG / R:CACATGCATTCCTACAAGCAC / (TG)22 / 213 / 57℃ / HQ650355
Hdi361 / F:ATCCACACCTTGCTGGATTG / R:AGAGAGGCGAGTGGTCAGTCT / (CA)36 / 168 / 57℃ / HQ650314
SupplementalTable 1 (Continued)
Locus Name / Primer Sequence(5'-3') / Repeat Motifs / ExpectedSize(bp) / Tm(℃) / GenBank
AccessionNo.
Hdi366 / F:CGAGTTCGATTCTCTACACGG / R:CGGCAGTCGTCAATAGCC / (GCC)5 / 155 / 57℃ / HQ650254
Hdi3-101 / F:CGACTGTTGCCTTTTTGTTG / R:GCAACCACTCACACACATCC / (TG)9ttt(GA)17 / 192 / 58℃ / HQ650465
Hdi3-109 / F:AGTCCAAATGTGTTCGCCTC / R:GAATTCAACGATTAGGGCCA / (TG)32 / 238 / 58℃ / HQ650475
Hdi3-111 / F:CGGCACATTACTGCATCAGA / R:GGGTGCCCAAATTACCTTCT / (GTA)6…(AGT)6…(AGT)10agac(GTA)9aa(AGT)9 / 264 / 58℃ / HQ650522
Hdi3-115 / F:GGCAACAGCAGTAGGGGTAG / R:CTTTTACGACAGGCATGGGT / (CAG)7tagt(AGC)9 / 271 / 58℃ / HQ650450
Hdi3-117 / F:CCCAGACAAAGGGGTGTCTA / R:GACGTTTCATACCAGCCGAT / (TGA)14(TGG)7 / 276 / 58℃ / HQ650496
Hdi3-119 / F:ACTTGTGAAATCCCCAGACC / R:GCTGTGCAAAACCAATCTCA / (GA)24a(AG)32…(AG)7 / 236 / 58℃ / HQ650483
Hdi3-123 / F:CGGGGAATATCTGCAACTGT / R:TGGTACCGCGTACGTAACCT / (AC)13…(AC)9 / 263 / 58℃ / HQ650520
Hdi3-129 / F:CTGGCGCTGTGACAAAGTTA / R:TGGTGTACATTAGCCCATGC / (GT)11(GC)6 / 214 / 58℃ / HQ650489
Hdi3-136 / F:CCTTGGCATTTGATCAGGTT / R:AATGTCACTGCCACCATCAA / (AATG)9 / 209 / 58℃ / HQ650499
Hdi3-145 / F:ATGTTCCGCAGTTTCCTGTC / R:GGCATCCTTTCTCAAAGCAC / (GT)17 / 270 / 58℃ / HQ650488
Hdi3-148 / F:AAGCAATTGTTGGAAGCACA / R:ATCCAGACGACGAGGCATAC / (TG)27 / 161 / 58℃ / HQ650516
Hdi3-158 / F:TATTTGAGCATCGGTTGTCG / R:GCTCGTAATGGCCTTCTCAG / (AC)10(AG)32 / 280 / 58℃ / HQ650495
Hdi3-169 / F:GTGGTCCACAACACAACTGG / R:GTTGGTCCCGAGACAGTCAT / (AGC)7 / 271 / 58℃ / HQ650464
Hdi3-170 / F:TGACGACAGATCAGCTGGAG / R:ACACAGGCTTACACTGCACG / (CA)8…(CA)14 / 227 / 58℃ / HQ650457
Hdi3-178 / F:ACCCATGGATATGGACAGGA / R:CTCACCCCCTACCACTAGCA / (ATC)18 / 140 / 58℃ / HQ650451
Hdi3-181 / F:TAGCATAGCAAGGGCAATGA / R:GTCCCCTTGTGACCTTGAAA / (AC)19 / 227 / 58℃ / HQ650440
Hdi3-182 / F:AAGCCTTGAACGAAGCATGT / R:GAGTTAGTGCGGTGCTGTCA / (GT)51 / 191 / 58℃ / HQ650526
Hdi3-189 / F:CCTGGAAATGCGAGAGAACT / R:AACGTGAATTTCGACCTTGG / (TATG)16tga(GT)9(GTAC)9 / 278 / 58℃ / HQ650481
Hdi3-193 / F:GACACGTTCCACAGCTTTCA / R:CGTGGTATCACATGGACCAG / (AC)25 / 279 / 58℃ / HQ650513
Hdi3-198 / F:TATCGGGCTAATTTTTGGGA / R:CTTAAGACCTCAAGCCGCAG / (TG)24…(TG)14 / 209 / 58℃ / HQ650503
Hdi3-199 / F:TAACTCGCTCCTCTGGCTGT / R:TGGTATGTCTTGCATGTGCC / (AC)7aat(AC)8aat(AC)11 / 174 / 58℃ / HQ650460
Hdi3-19 / F:ACCGCATTCAGCATTATTCC / R:ATGTTGGTTTTATCCTGCCG / (AC)22 / 197 / 58℃ / HQ650494
Hdi3-201 / F:AAGCGACCGTGCTCTAGTGT / R:ACCAGTGACAGAACCAACCC / (TG)29 / 229 / 58℃ / HQ650501
Hdi3-202 / F:GCCAGATTACAAACTGTGTTGA / R:AAATGCTGCTAAATTAAGAAACACAA / (CGA)5 / 279 / 58℃ / HQ650524
SupplementalTable 1 (Continued)
Locus Name / Primer Sequence(5'-3') / Repeat Motifs / ExpectedSize(bp) / Tm(℃) / GenBank
AccessionNo.
Hdi3-204 / F:TTGAGATGACCTCGTCGTTG / R:TTGCTGACTGAACGCCATAC / (AG)6,(GT)32 / 248 / 58℃ / HQ650480
Hdi3-205 / F:GGGTGACACTGGTCTCTCGT / R:CCATGTCACGTCAACTCACC / (TCG)7…(ATC)5 / 261 / 58℃ / HQ650511
Hdi3-206 / F:TGTACAACTGCATCGTCCGT / R:TTCTGTTTATCCCGAGCACC / (TG)7ta(TG)7…(GT)10 / 253 / 58℃ / HQ650507
Hdi3-20 / F:TATATTGGGCAAATCCGACC / R:TTGGTTGACAGTCGCCTAAA / (TG)13…(TG)20ta(TG)17 / 247 / 58℃ / HQ650477
Hdi3-210 / F:TTTAACCATTAGGCCACCCA / R:GGCCATGGTAACCCCTTAAT / (GTA)12 / 253 / 58℃ / HQ650455
Hdi3-218 / F:GCACATCAAAAATGGGGTTC / R:CGAAACCACGATAACAGTGA / (TCG)6at(GTA)14,(AGT)6aga(AGT)14t(GTA)7 / 236 / 58℃ / HQ650478
Hdi3-221 / F:TCAAGTGTGACATTCCTGCC / R:TAGGCCCAGTCTGAACAACC / (AC)14…(AC)12 / 237 / 58℃ / HQ650462
Hdi3-223 / F:GCGACCAGAGACTAGAGTACCA / R:GGTGACGTGTAACCTTGCTG / (AC)19 / 232 / 58℃ / HQ650471
Hdi3-22 / F:CTGCACGTTGAGAGTATGACG / R:GCGATCGTGAAGGTGTACTGT / (GT)21 / 224 / 58℃ / HQ650470
Hdi3-234 / F:TGATCGCTGGTTCCTATTCC / R:TGATTATTTCAGGCCGTCGT / (CA)38 / 229 / 58℃ / HQ650459
Hdi3-236 / F:TCAGTGAATGGCAGGTTGAC / R:ATCATGGGGGAAGACACAAC / (TG)6(TGTC)5…(TG)7tt(TG)18 / 267 / 58℃ / HQ650449
Hdi3-237 / F:GCTGGTACATGTGGTACACGA / R:CGATGATGATGGTGATGATGA / (CAT)9…(CAT)5…(ATC)5 / 340 / 58℃ / HQ650521
Hdi3-241 / F:TTAGCAACACGAATCATGCAG / R:ATTCTCACAGTGGCCAATCAC / (GC)10(GT)15 / 153 / 58℃ / HQ650461
Hdi3-242 / F:GGATACACCGTCTCAAGGGA / R:GCACTGTTTCATCGGCACT / (GT)10t(TG)18 / 279 / 58℃ / HQ650510
Hdi3-244 / F:ATGCTGCACGTGTTCATGTT / R:CGGGTTCATCTGAATGGTTC / (GT)18at(GA)45 / 190 / 58℃ / HQ650447
Hdi3-29 / F:CGCTTACTTGCACAACACCTT / R:GGAGCGTTAGATGGATCATTG / (TAG)15(TTG)10 / 381 / 58℃ / HQ650492
Hdi3-42 / F:GGTCTCGATTATCTGCCGAA / R:GCGATAGGTATCGACTTCGTG / (TG)40 / 309 / 58℃ / HQ650493
Hdi3-44 / F:ATGCGGATGTAATGGTGGTT / R:TTTACATCAACGGCATGAGC / (TG)15 / 203 / 58℃ / HQ650456
Hdi3-52 / F:TCGTAAACGTGTTCTCTGCG / R:TCGCGAAAAGTGTAATATCGAA / (GT)34 / 228 / 58℃ / HQ650500
Hdi3-54 / F:TTACCGTAAGCGTGTGCATC / R:TGCTGCTGTTGCTGCTTTTA / (AGT)6ga(TGG)11,(CAG)6 / 257 / 58℃ / HQ650446
Hdi3-60 / F:GCCGGTGGTTAACTTGGTAAT / R:TCAAGTCCGGACAAGACTATCA / (AAC)16 / 289 / 58℃ / HQ650514
Hdi3-63 / F:GCCGCCCGTTTTAAGTAAC / R:GGTGGTCCGGTAACACATAGA / (TG)22 / 273 / 58℃ / HQ650491
Hdi3-64 / F:CGGATGTGGTCCTCAAGAGT / R:AGAAACGGGGGCAGAGTATT / (ACG)5 / 260 / 58℃ / HQ650454
Hdi3-75 / F:CCGTGCGTGTTTGTGTTAGT / R:GTGCACAAAGACACGTGGAC / (GT)15,(GT)6,(GT)7…(TG)9 / 270 / 58℃ / HQ650508
Hdi3-78 / F:TACCTATTGTCCCCTGGCAA / R:AGCTCCATGTTGCAAGGTTC / (GT)15(GTG)6 / 248 / 58℃ / HQ650531
SupplementalTable 1 (Continued)
Locus Name / Primer Sequence(5'-3') / Repeat Motifs / ExpectedSize(bp) / Tm(℃) / GenBank
AccessionNo.
Hdi3-81 / F:GGGAGTAAGACCCATTCAGGA / R:GGTGAACACCCACATTCACA / (GT)30 / 238 / 58℃ / HQ650506
Hdi3-96 / F:GGTTGCTGTTGATGATGGTG / R:GGGGTAGCCTGATAGCATGA / (GAT)5…(ATG)5…(TGC)6 / 237 / 58℃ / HQ650468
Hdi3-98 / F:CCGCGTACAAAGCCTATCTC / R:CGCCGATGAGGGATATACTG / (GA)28(GT)14(GA)9,(TCAC)6 / 148 / 58℃ / HQ650498
Hdi3-99 / F:CATCTTGTTGTTAGCGCTCG / R:CGACGTGTTATGTCCATTCG / (GT)29 / 274 / 58℃ / HQ650482
Hdi101 / F:ACCACCACCACCTCCACC / R:TGTGATGGCTGTAGTGTGCC / (CAC)7(CAT)14 / 143 / 58℃ / HQ650385
Hdi103 / F:GAGTGTTCTACCTCTCGCCG / R:GAAGCATTGTTGAGGGAGGA / (GT)29 / 204 / 58℃ / HQ650370
Hdi129 / F:GTTCAAGACACCGACAGGTTC / R:GACGACAACATCAACAACAATG / (TC)28(AC)14 / 212 / 58℃ / HQ650313
Hdi130 / F:ACAAGGACAACAATCCACACC / R:TACGTAAGATGGTGGTCAGGC / (AC)34 / 217 / 58℃ / HQ650414
Hdi153 / F:AAGGTCAGCCAGCGTACAGT / R:CTTTACATTACTGGGGGCGA / (CA)11 / 279 / 58℃ / HQ650258
Hdi155 / F:TATTGAGGAAAATCGCCCAG / R:GCAACTATCCCCAAGAACCA / (CA)31 / 183 / 58℃ / HQ650293
Hdi183 / F:ACATTACGACCGAGCACACA / R:ACCAGCACCAGCTCAAGG / (GCTGGT)10,(GCTGGT)5,(TGGTGC)9 / 261 / 58℃ / HQ650326
Hdi18 / F:AAATGAGTACTCTCCCCGCC / R:CAACTACGGGCTTTTCCAAC / (CT)23 / 198 / 58℃ / HQ650285
Hdi193 / F:AAGGAAGGAAAACACCAGTTG / R:AAGGCCAATTTTATCCCAGG / (TC)6c(CT)7cccc(CT)10(CA)28 / 258 / 58℃ / HQ650323
Hdi197 / F:TTAAATTCAGCACACGCACG / R:GGGCACAGTTGGAAACAAGT / (CA)22 / 182 / 58℃ / HQ650331
Hdi287 / F:ACATGGATTCGGAAAACGTC / R:AACCCAAATTGCTGCCATAC / (CA)31 / 205 / 58℃ / HQ650357
Hdi288 / F:CATTTTCGCAACAGATTCCC / R:CAAGGACGGGCTTCAAAATA / (TG)27(TA)6 / 216 / 58℃ / HQ650337
Hdi314 / F:TTTACGCCGCTTTAAGCAAT / R:CCCTGGGGTGTGATTTAAGG / (TAG)5…(AGT)5…(TAG)5…(AGT)7…(AGT)6…(AGT)7…
(AGT)6…(AGT)5…(AGT)6…(AGT)8…(AGT)7,(AGC)10 / 262 / 58℃ / HQ650303
Hdi332 / F:TTTGTTTGTGCAACATTCCC / R:TTGGCAACTCCCTGGTAAAC / (TG)15 / 219 / 58℃ / HQ650353
Hdi348 / F:CGATGTTTGCATCCATTGAC / R:AAACCAGAGCGAAGACGAAA / (ATC)8(AGC)9 / 272 / 58℃ / HQ650375
Hdi34 / F:CTCTCCGTGCAGACATTGG / R:GCGCCTTAAGGAAGAAGTCA / (CA)21 / 264 / 58℃ / HQ650425
Hdi355 / F:ACGGCCATTCACCTTCATTAC / R:GGACCTGGAGATCTGTCGTG / (CA)37 / 226 / 58℃ / HQ650374
Hdi363 / F:AGTACCACTGTCCGACACCC / R:TTTCAAAATCCACTTGACCAAA / (GT)17gcgc(GCGT)8 / 271 / 58℃ / HQ650382
Hdi364 / F:TATCAACAGACCGCCTCCATA / R:TCAGTCACTGTGAGCATCTCC / (CT)27 / 144 / 58℃ / HQ650252
Hdi38 / F:CTCACACCGCCAACATACAC / R:CCATACAAGGATCATGGGGT / (AC)10t(CA)20 / 280 / 58℃ / HQ650333
SupplementalTable 1 (Continued)
Locus Name / Primer Sequence(5'-3') / Repeat Motifs / ExpectedSize(bp) / Tm(℃) / GenBank
AccessionNo.
Hdi401 / F:TCTTTCAAAATTGCATGTCCA / R:ACGAAGCCAAACTAAAGCCA / (GT)27 / 280 / 58℃ / HQ650240
Hdi406 / F:AAGGACTTAGGATAATCACACTGGA / R:TGTGAGAGAGGCGGTGATACT / (AC)11(AG)7…(GA)11 / 223 / 58℃ / HQ650305
Hdi423 / F:CCGTAGGGTTCAGGGACTTA / R:TATTGGCATTCGCTAACACG / (TG)25 / 184 / 58℃ / HQ650262
Hdi424 / F:TCAAACTGACCGACAAAACG / R:GCTGGCTGGAGAGAAACAGT / (AC)18 / 240 / 58℃ / HQ650320
Hdi455 / F:GTGACAAGGGCAATTCAGGT / R:CCGCCCCTATGTTTGTATGT / (ATG)7…(G)10tag(TGA)14…(TGA)8…(TGA)10…(GAT)8 / 259 / 58℃ / HQ650277
Hdi456 / F:CCGCAGCCTTATAGATCGAG / R:ATGTGAGTGACAGAACCCCC / (TG)37 / 205 / 58℃ / HQ650377
Hdi464 / F:CACTTGGGTGTCCCACTTCT / R:GGTTACCAAGCCATGTTGGA / (GCA)7 / 176 / 58℃ / HQ650264
Hdi466 / F:CACTTTTCGATGTTGCCAAA / R:GTTCGTTGGTTGTCGCTTTT / (AC)32 / 258 / 58℃ / HQ650339
Hdi48 / F:tAGCAGAAGCAACAAGCACAG / R:ACCTTGTTGCAGAGGATTGTG / (CA)6,(CA)30…(CA)6…(CA)7…(CA)7,(CA)7 / 164 / 58℃ / HQ650239
Hdi50 / F:TAAACTCCACTCCGCTCCAC / R:GAGGTGACTGAGTGCGTGAA / (TCCAC)5 / 141 / 58℃ / HQ650368
Hdi53 / F:TTCTTTCCTTGTTCAGCAGTCA / R:GAAGCCTCCTGTCCTCTTGA / (CAT)10…(CAT)5…(CAT)13 / 253 / 58℃ / HQ650426
Hdi64 / F:GGATCGTGAGGCTGGTACAT / R:GCAAATGTCGTCTGTTGAGG / (AC)9…(CA)9(CAC)8 / 260 / 58℃ / HQ650282
Hdi68 / F:CATGCCTTCGTTCAGTCCTT / R:TGAGTGAAGGAGTGTCTGAGTGA / (TCAC)5 / 127 / 58℃ / HQ650255
Hdi69 / F:ACACAGGTTCCAGTTCAGCC / R:CAGAAGCCATCAGGTGCC / (AC)21 / 210 / 58℃ / HQ650234
Hdi74 / F:TGACCCCGACCTCTGAATAC / R:GTTGTCGGGGAGCAGTTGTA / (CA)28t(AC)25 / 265 / 58℃ / HQ650295
Hdi80 / F:TGAGAGAGAGCCTCACTACCG / R:TGGACAATGTTGGACAAGCTA / (AC)25 / 203 / 58℃ / HQ650433
Hdi81 / F:TCTCGAGAAACGTTTAGTGTTTGA / R:ACATGGCAAATTCGATGGTT / (AC)32…(A)11…(A)20 / 271 / 58℃ / HQ650369
Hdi98 / F:GCATGATGTGTCTGAGTGGG / R:CGTGAATCTCGCTGTTTGAA / (CA)16 / 216 / 58℃ / HQ650388
Hdi99 / F:ATTCAAACTATATGGCGGCG / R:GCTGTATGTGATCCGTCCCT / (ATG)5acgg(CGA)6(TGA)6 / 258 / 58℃ / HQ650342
Hdi3-77 / F:TTTTGTGTGCTGGCGAATAG / R:GGAAGTAAATCCAGGCGAAA / (TCA)18(ACA)11 / 280 / 59℃ / HQ650466
Hdi133 / F:CGACTCCACCTTCCTATTCTCT / R:CAACGGAGATGGTGGTCG / (CTA)8(CTG)7 / 214 / 59℃ / HQ650341
Hdi137 / F:CGGATCCGATTCGTCCTAATA / R:CATATCACTTGTGCCACCGT / (TG)30 / 226 / 59℃ / HQ650430
Hdi140 / F:TGACATATTCGCCACACTGAC / R:GGCATAGGTAGCCACCATCTA / (TC)7tg(TC)6tg(TC)22 / 189 / 59℃ / HQ650318
Hdi142 / F:TCAGCATACTGTGTCTGAGTGG / R:GGTGAGTTGAGGTGAGGTGAG / (AC)20 / 202 / 59℃ / HQ650308
SupplementalTable 1 (Continued)
Locus Name / Primer Sequence(5'-3') / Repeat Motifs / ExpectedSize(bp) / Tm(℃) / GenBank
AccessionNo.
Hdi152 / F:TTCGGTCCAATAACGTCCTAA / R:GGTATCTCTTGGAGGTCGGTA / (CA)30 / 218 / 59℃ / HQ650421
Hdi154 / F:TTATACTTGCACACACGCACA / R:AAGTGGACCTTCTGTTCAGGT / (AC)15t(CA)9 / 187 / 59℃ / HQ650298
Hdi178 / F:GTACATATACTTGCCAGCCGC / R:AAGAATTCAGGAGGTGTTAGA / (TG)8…(GA)33 / 260 / 59℃ / HQ650435
Hdi210 / F:ACAACTCAGCCTCGCCG / R:TTCTGAGGCACTGACACTT / (GT)23 / 165 / 59℃ / HQ650416
Hdi240 / F:GATGATGATGGTGCTTCTGGT / R:TCTTAATGTCATGCAGCCTCA / (TGG)7(TGC)5(TGA)7 / 196 / 59℃ / HQ650272
Hdi243 / F:AGCGCCTTGAGCATACAATAG / R:ATGCCGAATTAGACGACATCA / (TGA)15 / 186 / 59℃ / HQ650340
Hdi244 / F:GTGCTTGTGTGATGCTCCAA / R:CGTCTCGTGGTATGTGTGTGT / (CA)11cgt(AC)11 / 112 / 59℃ / HQ650392
Hdi249 / F:AACAATATCACGCGGACACAT / R:TTACAGATTGCCGTTATGCAG / (GAC)6 / 122 / 59℃ / HQ650418
Hdi251 / F:ACACACACAGACGCGCAC / R:AACTACCGCATCACTATGG / (AC)18 / 203 / 59℃ / HQ650410
Hdi264 / F:GAGGATGGAATTAAGATGTGGTG / R:CAACACCTACCACTGCAGCTA / (CAG)9 / 167 / 59℃ / HQ650352
Hdi267 / F:TATTCGCAAGGTCTCTGCTTC / R:TTCTGTCTGAGTATCTTCGCAA / (ACA)12 / 154 / 59℃ / HQ650259
Hdi275 / F:TCCGGAATAGACGAACAACAC / R:GCTGACACAAGACGACGAGTT / (CA)21 / 190 / 59℃ / HQ650422
Hdi277 / F:GATAAGAGAACCTCAGAGGCCA / R:CGgATGGTAAGTGACAGTACGA / (TC)21 / 152 / 59℃ / HQ650253
Hdi279 / F:CGGAATAACACGAGTTACGGT / R:TGCCAACCATTGCCAAC / (GTGC)5…(CTGT)5(TG)25 / 282 / 59℃ / HQ650283
Hdi280 / F:GACGTCTTGAATGCCGTACTC / R:CGGTTGGTTGAGTGAGTTGAT / (CA)17 / 174 / 59℃ / HQ650354
Hdi284 / F:GAAGCGGTTGTATGCCATTAG / R:TTGTGAAGGTGTATGCCATT / (CA)17…(CACG)6 / 200 / 59℃ / HQ650350
Hdi290 / F:TGGTCACGGTAGTACACACCA / R:TCAGACaGCATGACAGATtcg / (AC)22 / 120 / 59℃ / HQ650335
Hdi292 / F:CTACTAATACTACTGCTGCTGCT / R:TACACGGCACTGACACAAGAC / (TAC)5…(ACT)6(ACG)5 / 133 / 59℃ / HQ650398
Hdi294 / F:GTCCGAGCTCGACGTTGTT / R:AAGCAGTAGGTGAGTGCACAA / (ACT)8(GCT)6acta(CTG)8ctat(TAC)13 / 217 / 59℃ / HQ650411
Hdi295 / F:AACTGTGTCTTGCTCACATT / R:CGTAAGCTTGTATAATGTGTCGG / (TC)30 / 201 / 59℃ / HQ650306
Hdi296 / F:CACATGCAGGAAGTGAGTGTG / R:TCATACATGGCGTATTGTCCA / (TC)8(AC)8agc(CA)24t(AC)6 / 226 / 59℃ / HQ650395
Hdi298 / F:GCATTGATGCTGGTCACCTAC / R:ACGGCGCATTATCTGTAACAC / (AC)25 / 166 / 59℃ / HQ650302
Hdi299 / F:GCTCGTCACGATGCAGAAT / R:GAGTGGCTATGGTCTGGCTTC / (C)19,(GTG)7…(GTG)6…(GTG)5…(TGG)5…(GTG)7 / 115 / 59℃ / HQ650432
Hdi301 / F:CGCTTAGAGTAACATGAGCGA / R:AAGTGCACTGTACGGAAGCAT / (AC)33 / 219 / 59℃ / HQ650343
Hdi304 / F:CTCACTCACACACTCACACGC / R:GGATCGCTCATTACCTTGTGT / (CA)15,(CA)21 / 210 / 59℃ / HQ650250
SupplementalTable 1 (Continued)
Locus Name / Primer Sequence(5'-3') / Repeat Motifs / ExpectedSize(bp) / Tm(℃) / GenBank
AccessionNo.
Hdi3-1 / F:GGTCAGGCTCACTGACTTGTT / R:TGGTTATGGTAGTGATTGCAGG / (CCA)9,(ACT)5at(TAC)12c(ACT)6,(CTA)10…(ACT)7 / 338 / 60℃ / HQ650458
Hdi3-108 / F:CCTATTGTACCCCGGGTCTT / R:TGCCTGGAAGCCATAGTACC / (CAG)6(TAG)14 / 175 / 60℃ / HQ650443
Hdi3-168 / F:GCAGTGAAGGTTAAGCAGGC / R:TCTTGCAAGGAATGCAGATG / (GT)37 / 238 / 60℃ / HQ650445
Hdi3-173 / F:ACGTGTGTGTGTTAGTGTGTGC / R:AACATGTGCACAGACACTTGG / (GT)7,(TG)6 / 299 / 60℃ / HQ650476
Hdi3-174 / F:CCCAACTGGCATTCCTATGT / R:TTGCCTACACGCTAACTCACA / (CA)14 / 254 / 60℃ / HQ650442
Hdi3-24 / F:CGTGTCATAGTCTGGAGGAGG / R:CCGTACAAGGTGTCTCAGTTG / (TG)13c(GCGT)7 / 392 / 60℃ / HQ650515
Hdi3-36 / F:AAGAAACTCAACCGAGGCAA / R:CGTACTCCCACTTCCGACAT / (CAG)6 / 156 / 60℃ / HQ650444
Hdi3-53 / F:GAGAACATCATGCTGGCGTAT / R:CCGAGGTGAAGGTATCTTCTGT / (TG)6 / 356 / 60℃ / HQ650534
Hdi3-71 / F:GGTGTATCGGAACGAAGAACA / R:GTATGAATTAGCGATCGTGGC / (AG)27…(AC)10 / 324 / 60℃ / HQ650525
Hdi3-76 / F:AGCAGCAGCAATAGCATCAGT / R:ACAAGCAGCCACACATACACA / (AGT)8…(AGT)7…(AGT)9…(AGT)6…(GTA)6(GTT)5…(AGT)5,(GTT)6 / 347 / 60℃ / HQ650530
Hdi3-87 / F:GGCTTGGAAGACTCATTAGCA / R:TGCATCCTCTTCAGTAGGTGG / (TCA)18(ACA)11 / 389 / 60℃ / HQ650529
Hdi3-88 / F:CAATCTGGTGTTCTGGCATCT / R:GCTAGTACCACTGCTGCTTCC / (AGT)21 / 274 / 60℃ / HQ650509
Hdi3-97 / F:ATATCCACTGCTGACTTGCGT / R:CTCTGCGAAGTGGAATAGCTG / (TG)27…(GT)23 / 315 / 60℃ / HQ650527
Hdi168 / F:GTGTCCCCTCCGTGATATTG / R:AAAGGGCCATATTGGGAGTC / (AC)24 / 230 / 60℃ / HQ650356
Hdi172 / F:CATCCAACTCCCTCTTCTGC / R:CACCACGTGCATGTCTTTCT / (AC)29 / 129 / 60℃ / HQ650364
Hdi236 / F:TTCCGCAAAGTGAAATGACA / R:GCGCACATGCAGTCATACTC / (AC)34 / 280 / 60℃ / HQ650349
Hdi321 / F:CACGCAGTCTCAAACGTGTC / R:ACTGCCTGCTTGTGTCTGTG / (AC)7 / 190 / 60℃ / HQ650347
Hdi350 / F:CACTTCCTGAACCCCTGAAA / R:ATACACACACATGCGCACAC / (GT)11 / 240 / 60℃ / HQ650424
Hdi367 / F:CACATCATGAGAGTTGCTGGT / R:AAGACAGGTTCAATTCTAAGG / (GA)41 / 233 / 60℃ / HQ650310
Hdi370 / F:AACCACATCCATGCATACAACT / R:CAATGTGCGTAGTACACGAAGT / (GT)9…(GT)9 / 182 / 60℃ / HQ650360
Hdi371 / F:TGATCACTTGATTGTCTGGTCC / R:GACCGAATGATCTGTTGACGTA / (GT)32 / 233 / 60℃ / HQ650363
Hdi380 / F:AACCATCTAAGAGCCAGAGCC / R:ACAGCCTGCAGTTAGCACAGT / (CTG)6 / 113 / 60℃ / HQ650429
Hdi388 / F:TCACCAGTCTTGTTATGAATG / R:CAGatAGGTGCTtcACTGTGG / (TG)33 / 154 / 60℃ / HQ650268
Hdi404 / F:CACTACTGCTGCTGCCACTAC / R:GGCTGTCGTGGAGTGATGTAT / (TAC)10(CAC)7,(CTA)7 / 185 / 60℃ / HQ650330
SupplementalTable 1 (Continued)
Locus Name / Primer Sequence(5'-3') / Repeat Motifs / ExpectedSize(bp) / Tm(℃) / GenBank
AccessionNo.
Hdi405 / F:CGTGCATGATGTATGACGTGT / R:GTTGCATCTATGTGCTGTGGA / (CA)6cgtg(CA)8…(CA)8 / 213 / 60℃ / HQ650405
Hdi419 / F:ATTGTGTGCCACCATCCAG / R:CTGCCTCGGACCTGTACCTA / (CA)29c(CA)8a(AC)11…(AC)9 / 241 / 60℃ / HQ650408
Hdi422 / F:CATTACATGGCAGCGGTCTAT / R:TGTTGTCATAAGTCAAGGACAGG / (GT)22 / 213 / 60℃ / HQ650336
Hdi427 / F:GCAGACTTCTGCTGCTGCTAC / R:TGCTTGTACATGCCAAGATCA / (GT)13,(ACT)5…(CTA)10(CTG)9(CTA)9 / 228 / 60℃ / HQ650251
Hdi428 / F:ATCAGAGACACCGCCATACAC / R:GGTCTGTATGACACACTAGCGG / (CG)7(CA)13 / 154 / 60℃ / HQ650249
Hdi430 / F:CGGAGACGACGTACATAACGA / R:CATGGATGCATATCGACAGgt / (GA)46 / 236 / 60℃ / HQ650321
Hdi433 / F:TACCTCACCTCACCATGCTTC / R:TGtgttgtGGAGGTGACTTGA / (CA)8cgcg(CA)6…(CA)7gat(AC)10 / 254 / 60℃ / HQ650420
Hdi434 / F:GAGTCGAATGACTGTCGATGG / R:AAGATTGACAGATGCGACTGG / (CACACG)5,(TGC)8 / 128 / 60℃ / HQ650287
Hdi435 / F:TTGCATTTTGATCGCTCAGT / R:CAGGAGGACAAGATTTGGGA / (GT)11 / 280 / 60℃ / HQ650297
Hdi441 / F:GCATCATCATCCTTCTCCTCC / R:CGTTCTCATTGTGTGGATTGA / (TCA)7(GCA)9 / 216 / 60℃ / HQ650294
Hdi445 / F:AACAGTGTCAACACTCGCATC / R:CTATAGCGCCTTGAACACGTC / (TG)28 / 194 / 60℃ / HQ650247
Hdi451 / F:GTTACATATCAGCAATATCACGG / R:GTCACGTCAACGAACGACC / (TG)23(AG)19 / 191 / 60℃ / HQ650383
Hdi453 / F:AATGGTGGTGGATGTAGTGGT / R:ATGACTGCTGCTTGCTTCATC / (GTA)10gtg(GTA)11…(GTG)5…(GTA)13(GCA)11 / 369 / 60℃ / HQ650431
Hdi460 / F:CACCCAGCATCTCCTGTTCT / R:CGGGGTGATGGCTAATGTAT / (CA)44 / 155 / 60℃ / HQ650434
Hdi463 / F:CACAGTCATCGTAACACATATGACA / R:GGAATCGAACTCTGCTCCAC / (ATC)16 / 200 / 60℃ / HQ650324
Hdi476 / F:TGTCGTTCGAGGTGAATAACG / R:CGACATTATCGTTCCGTAAGC / (CAT)5…(ATC)15 / 233 / 60℃ / HQ650316
Hdi52 / F:TCGAGCTGAGAATGACTGACC / R:AATCTCCGCAACCTTGTCAC / (ATC)6(ACC)5act(ATC)6 / 211 / 60℃ / HQ650315
Hdi55 / F:CATCCATCTCTGTCTCCAGATG / R:CTCAGTCTCCTGCATCCATGT / (GTG)6(CTG)5 / 137 / 60℃ / HQ650236
Hdi59 / F:TTAAGATTCACATCGACACATCG / R:GTGATGGTCTGCCATAACACAA / (CA)29 / 214 / 60℃ / HQ650238
Hdi62 / F:TATCATGCCAATCAGACCTCC / R:TGTTGTTGTTGTTGCTGCTGT / (CAG)8 / 165 / 60℃ / HQ650397
Hdi63 / F:ATGCTCACTGACGCACGTAT / R:GGTCAGTCTTGTATGGTTCGC / (AC)15 / 156 / 60℃ / HQ650291
Hdi67 / F:TCACGCATACATACACGAAGG / R:GACTGAGGAAGTGAGTGCCTG / (CA)24…(GCAC)7 / 196 / 60℃ / HQ650319
Hdi71 / F:TGCACGGCTGCTAATAGTCAT / R:TGCCATGATGTTGTTGGAATA / (AC)15 / 177 / 60℃ / HQ650263
Hdi72 / F:CCGCCGGAATAGCAGTACTAA / R:GGCGTTAGTGAGAGAGAGGCT / (AGT)6 / 184 / 60℃ / HQ650372
Hdi93 / F:AGACTCCTTGTCGTGATGGTG / R:AGAATTACCGTCCGAATCAGC / (AC)38 / 208 / 60℃ / HQ650376
SupplementalTable 1 (Continued)
Locus Name / Primer Sequence(5'-3') / Repeat Motifs / ExpectedSize(bp) / Tm(℃) / GenBank
AccessionNo.
Hdi96 / F:GCTACGTGCTGAGACCGATAC / R:ctggccgcgtgatctaaG / (GT)14 / 106 / 60℃ / HQ650404
Hdi273 / F:TACAACTGCGAGTGGTGAGTG / R:GGCTTGGCGTGACTAACCTAT / (TG)21 / 204 / 61℃ / HQ650260
Hdi300 / F:TGGTACCGGCTCATATCTCAC / R:GACAAGccgttCCTGTTACAA / (GA)55 / 277 / 61℃ / HQ650436
Hdi308 / F:GTTCTGTCATGCCGATCTGTT / R:CCAATGTGACTTCTTAACTAACGC / (TG)9,(AG)33 / 145 / 61℃ / HQ650292
Hdi3-121 / F:GATGATGTGACAGAGCGAGGT / R:TCACCACCAACTGTCATTACG / (TG)30 / 378 / 62℃ / HQ650486
Hdi3-203 / F:GTCCAGGTGTGTGTGAGTGTG / R:ACACACGTGTCATGGTTCTGA / (TG)6,(TG)6c(GT)6,(GT)6…(GT)6 / 284 / 62℃ / HQ650505
Hdi3-216 / F:AACCGGTTTCAAGCGTTATG / R:AGCGATGTCACCCTTAGGAA / (TG)11(AG)34 / 266 / 62℃ / HQ650441
Hdi3-232 / F:ATTGTCTGGTGCACAGGCTC / R:CACTTGTTGGCTTCATCCATT / (GT)25 / 261 / 62℃ / HQ650485
Hdi3-84 / F:CTGCCACCCTAACCCTAACA / R:AACACCAGCATCAACAGCAG / (TGC)5 / 201 / 62℃ / HQ650535
Hdi372 / F:CCTCACGTGCTTGTACCAGAT / R:GCAACAGCAGTAGTAGCAGCA / (CA)13…(CA)8…(AC)17…(AC)8,(TAC)8 / 114 / 62℃ / HQ650265
Hdi373 / F:CCATGGAGGAAGGAGAGGAT / R:CCACAGCTTCGACACAATCA / (GT)35 / 185 / 62℃ / HQ650351
Hdi378 / F:TATCACAATGTTCTGGCGTGA / R:GcATCGTTGTGTgaagGTTGT / (GT)6tgttgttgttt(TG)30 / 220 / 62℃ / HQ650402
Hdi409 / F:CTATCCTGGTAATAGCGCTGG / R:CTGCTGAAGGCCTGTTCTACC / (CAT)5…(CAT)5…(TCG)5,(ACTC)5 / 196 / 62℃ / HQ650312
Hdi411 / F:CACAACGCTTCCAGGAATATG / R:CATATCTTGCAGATGCAGCG / (CA)10(GA)13…(AC)9 / 214 / 62℃ / HQ650358
Hdi417 / F:CACTCAGTGTGAACATAACGTCTG / R:ACGTGGTCCGGATCATACAGT / (CA)42 / 250 / 62℃ / HQ650235
Hdi421 / F:GGAACTGTGAACTGTGTGATTC / R:GTGGTAGCAGTGTGTGTGTGC / (CT)58 / 203 / 62℃ / HQ650348
Hdi442 / F:TATTGCCACCGAGAGTACCAG / R:ATGATGCTGATGATGATGACG / (ATC)10…(TCG)6 / 211 / 62℃ / HQ650317
Hdi45 / F:CATGAATATCTGGAAGGCTGC / R:TTGCCGGAGAGTCTGAATG / (GCT)7 / 179 / 62℃ / HQ650237
Hdi56 / F:ACACACGCATGCAAGCAC / R:CTGGCCTTGTTGTCTGAAGAG / (AC)38 / 186 / 62℃ / HQ650278
Hdi75 / F:GTTCATTGTCGTAATATGCACAG / R:GTAGCCGTCACAGTTGCAGTC / (ATG)9…(GAT)11 / 273 / 62℃ / HQ650384
Hdi88 / F:TGACCAGTCTTCTTGTATGCG / R:AATCTGAGAGCACAACTCAGTG / (AC)21taac(ACACTA)5…(CA)6 / 245 / 62℃ / HQ650406
Hdi331 / F:TCAAATCAGCTGCACAATAGC / R:TGTGCAGGTGTGTGTGAGAA / (CT)9cg(CA)7,(CA)6,(CA)20 / 221 / 63℃ / HQ650359
Hdi415 / F:CAACTCACACATCAATCACACAG / R:ATATGCCGACTTGCTGAACAC / (GA)37 / 206 / 64℃ / HQ650311
Hdi203 / F:GCAGACCAGTAGGACCAAGAC / R:CCGTAAGCAATGCTGAAGAA / (GT)11 / 160 / 57℃ / HQ650391
Hdi272 / F:TGACTGAGTGACATACGTGTTG / R:CCTGAATATATGGCATGACGG / (AC)30ag(AC)9(CA)9 / 267 / 59℃ / HQ650409