LOCUS pXGPHLEO 6317 bp DNA CIRCULAR SYN 28-JAN-2001
DEFINITION pXG vector with PHLEO replacing NEO
AUTHORS Stephen M. Beverley
FEATURES Location/Qualifiers
CDS 3..878
/gene="'NEO/DHFR"
/product="NEO/DHFR-TS"
CDS 1287..1362
/gene="5'DST"
/product="5'DST"
CDS 1385..1501
/gene="6.2-l"
/product="6.2-l"
CDS 1971..2123
/gene="1.7-l"
/product="1.7-l"
misc_feature complement (2153)
/gene="T3"
/product="T3"
misc_feature complement (3147..4007)
/gene="Amp"
/product="Amp"
misc_feature 4877
/gene="SP6"
/product="SP6"
CDS 4941..5140
/gene="3'13K"
/product="3'13K"
CDS 5635..5883
/gene="NEO/DHFR''"
/product="NEO/DHFR-TS"
CDS 5894..6268
/gene="PHLEO"
/product="St. hundustanus bleR"
BASE COUNT 1333 a 1897 c 1693 g 1394 t
ORIGIN
1 CTAGTGATCC CGCCCGCACC CGCGCGCGCT CAGGCCGCGT GTCGTCGTGC TCTTTCCATT
61 TTTTTTTTGT GTGTGGTGGT GATTTGAGCT GCTCTCCGTT GTGTGCTGGG GACCCTCCTT
121 CCCTCGATCT CCTCGTGCGG GGTCTCCGAC GCGCAGACGC GGTGCGTGCG AGCGTGCACG
181 TGCTGTCGCC GTGGCTGTAG TTGCGAGCGG AGAGAGAGAG AGAGGGAGGG AGGGAGGGCA
241 GAGGGCAGAG GACATGCGGG TGGGAACGTG CACCGGCCTC GTCTCACGCA GCTGGAGCCC
301 ACGAATGCCA CCACCACACC CTTCTTCCCC CCCCCTCCCT CTTCTTCCCA CGGCGGCGAC
361 GACGACGGGG GCTCAGCTCA CGCTTCTGTA GGGTATTATA TTAAAGCACA TGTGCGTGCT
421 GTGCTTCGGC TTCTTGTTGG TGGTCGTTCG CTTTCAAGTC CACGACTCCT GCCGTTGCTC
481 ACCGCCGGGC TGTCTTCTTT CGCCCCTCCC TCGGTGCCTC TTCCGCCGCC GCACGCGTGC
541 GCTGATCACG CGTCTTGCGT GATGTGCCTG TGTGTATACA CACCGTGCAC GGAGAGAGCG
601 AGCGAGCGAG AGAGAGAGAG GGCGAGAGGA AGAATGGCCG GTGCCTCGCG TGGGCAAGCG
661 TGCGCGAGTT TGTCGTGTCG CTGCGCCGCG TGGATGTCGC GCAGCGCAAG ACCCCGTGCG
721 CCTGAAACGT GAAGGGAGGT CGAGAAGCGT GCCGCATGAG GCCTTAAGGC AGGAAAGTGA
781 AAAGAGCTCG ACGCGAGTGC AGCACGCGCG ACATCGAACA GGACGGAATA GACACTCAGC
841 CCCTTCCCCT TTCAAAAACT GAATGGACGG ACATCGTAAC GCGCTTCTCT CCCCTCCACT
901 CCCCCTGTCC CTGTCTCCTT GACGTGTGCT GCTCTCGTGA GCGACAGATG AAGCGAGGCG
961 TAGAAGGAAA GGGGGGGGAG CGAGGCCTGC ACACACACAC ACACACACAC ACACACACAC
1021 ACACACACAC ACACACACAC ACACACGAAA AGCAAGTTTG AGGCAGACGT GGTTTCACAC
1081 GATTCCTGTG CAAGAAGCGT GCCTGCGTGA CGGTGTGGCT GTGTATGCAT GCGTGAAGGG
1141 TTACCGCAAC GATGCGCAAT GGACTCCCCC GCTTTCCATT TCGTCACCTT CCGCCTCTCT
1201 CTCTCTCTCT CTCACCATCT ACGCGTGCAC ATCATCAACT GTCTCTTGTC GGTGCTCACC
1261 ACCCTCAACC ACCCCTCACT TTCAAGGCTT CCCGAACGCA CACAAAAGGC GTGAAAACCG
1321 CTCGCGTGTG TTGAGCCGTC CACCGTAGCC CTCCCCCTGT CCCCGGGGGA TCCACTAGCA
1381 GATCCTCGTG TGAGCGTTCG CGGAATCGGT CGCTCGTGTT TATGCCCGTC TTGGTGTTGT
1441 GCTCGCAAGG CGGTGCAGCA GGATACCGTC GCCCTCCTCT CTCCTTGCTT CTCTGTTCTT
1501 CAATTCGCGA TCTCACAGAG GCCGGCTGTG CACGCCCTTC CTCACCCTCC TTTTCCCACC
1561 TCTCGGCCAC CGGTCGGCTC CGTTCCGTCT GCCGTCGAGA AGGGACGGGC ATGTGCAGCT
1621 CCTCCCTTTC TCTCGCGCGC GCATCTTCTC TTGCTTGTGG CACTCACGCT CATGCGTCAA
1681 GGCGGCCCCA CGCGAGCCCC TGCGCTCCCT TCCCTCTTGC GCATCCGTAG CCGGACTGGT
1741 CGATGCGCAA GGCCGGCATG AAGGAGCGCG TGCCCTCAAG AGCGGACTAT CATGCCCTAC
1801 GTGGGCCACG CAGCGATGAG GCCGGCTTCG CGGAGATGCG TCACGCACGT GCCAGATGAT
1861 GCCGTACGCC TTCCTTGACT TGCGCCCCCC TCTCTTCCTC CGTCTCTCAC TCTCTCTCTC
1921 TCACACACAC ACACACACAC ACACACACAC AAAGCTTCCT TGCACACAAC GTCCCCCTCG
1981 TTGTGCGGGC TGACGCCTCC CGTTGACAAC TTGCCTTACG CACGCAGCAC TCGGCCAGCC
2041 AACGAGGCCG CAGCGGCATC ACGTGTATCT TTCCACAGAA ACCGCGTGCT TTCGGATACT
2101 CTAGTATTTT TGTTTGTGGT CGAATCTCCC TTTAGTGAGG GTTAATTAGT CCTGCATTAA
2161 TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG
2221 CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG
2281 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA
2341 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC
2401 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA
2461 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG
2521 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT
2581 CAATGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT
2641 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG
2701 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC
2761 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC
2821 ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA
2881 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC
2941 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG
3001 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
3061 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
3121 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
3181 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
3241 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA
3301 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
3361 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
3421 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
3481 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
3541 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
3601 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
3661 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
3721 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
3781 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
3841 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
3901 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
3961 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
4021 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
4081 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC
4141 GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC
4201 TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG
4261 ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA
4321 GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG
4381 AGAGTGCACC ATATCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA
4441 GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC
4501 CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA
4561 GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA
4621 CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCTGGCTAG
4681 CGATGACCCT GCTGATTGGT TCGCTGACCA TTTCCGGGGT GCGGAACGGC GTTACCAGAA
4741 ACTCAGAAGG TTCGTCCAAC CAAACCGACT CTGACGGCAG TTTACGAGAG AGATGATAGG
4801 GTCTGCTTCA GTAAGCCAGA TGCTACACAA TTAGGCTTGT ACATATTGTC GTTAGAACGC
4861 GGCTACAATT AATACATAAC CTTATGTATC ATACACATAC GATTTAGGTG ACACTATAGA
4921 ATACAAGCTT GGGCTGCAGG TCGACGGGGT GATGGAGAGA ATGGTTGACC ATGAGGACGC
4981 CGATGGGCGA TGCGCCAGCG CGTGCTTGCG TACTTCAATC GTTTTTCATC TTGATTCAGA
5041 GCTAAGTAGT AGCACACCAC AGCAAGCGTA ATCATGTAGC ATGTATGCAG GTGCACGAGT
5101 AGGCACTCTC TGTCTCTGTC CGGCATCTAT GATGCTCTCC GGCCGCGTGC GCGCGACGCC
5161 TCTGTGTGAG TGCTCGTGTG AGTGTGTGAG AGCTGTCCAG GCCTTTCCTC TGCCTACTCG
5221 TCCCACGCTC AGGCAGGCAC AGAGGAATGA AAAGGAAGAC GAGGACGCAA CGCCAGGCGC
5281 ATCGACATCG CGCACGTGTT TGCGGTGTAC TGTGCGTCGA TGATCGAGCG GCCAAGCGTC
5341 CAGCTTCACG CGAGGAGGAC TGCATCGCTG CTCCTGTGCG CGTGCGTGCA CGCGTCAAGG
5401 CACGCTCGAT TCGTTCCCAG GGTGGATACC CTCCCCCCTC CCCCAGCTGC AGACAACCCC
5461 CACCCTCTCG AGGCTTGCAC CCAGGCTCGT CAGTCTCTCT ACCACTTTCT GCCTTCTGTA
5521 GTGCACCCCA CCCCTGCATT CTCCTCTCTC TCACACACAC ACACACACAC ACACACACAC
5581 ACACACACAC ACGTACACAC GTACACACAC ACGTGCGCAT GCTTGACGCA TACGCGACGA
5641 ATTCGAAAGC TCACCTCATT CCTCCCTCCT CACACCATCA TCGGCATCCA TAGAGACACG
5701 TGCGCGTAGA ACGATACAGC TCACGCGCAC AGAGAGGCGT GTTCTGGTCG TGCGCATCAT
5761 CGGCACAGCA CTGGCGGACG AAACTCGCAC ACAGGCACGC CGCCTCCTTT CACCCGTCAT
5821 AGATAGTTGA ATTAGACGCC CTCCTCCTCC CTCATCATCG CCGTCGTCAT CCGGGTCCGA
5881 GCACTAGATC ACCATGGCCA AGTTGACCAG TGCCGTTCCG GTGCTCACCG CGCGCGACGT
5941 CGCCGGAGCG GTCGAGTTCT GGACCGACCG GCTCGGGTTC TCCCGGGACT TCGTGGAGGA
6001 CGACTTCGCC GGTGTGGTCC GGGACGACGT GACCCTGTTC ATCAGCGCGG TCCAGGACCA
6061 GGTGGTGCCG GACAACACCC TGGCCTGGGT GTGGGTGCGC GGCCTGGACG AGCTGTACGC
6121 CGAGTGGTCG GAGGTCGTGT CCACGAACTT CCGGGACGCC TCCGGGCCGG CCATGACCGA
6181 GATCGGCGAG CAGCCGTGGG GGCGGGAGTT CGCCCTGCGC GACCCGGCCG GCAACTGCGT
6241 GCACTTCGTG GCCGAGGAGC AGGACTGACC GACGCCGACC AACACCGCCG GTCCGACGGC
6301 GGCCCACGGG TCCCAGG
//
B3324 pXGPHLEO