UCSF Lab Standard Operating Procedure

UCSF Lab Standard Operating Procedure

Chemical Process, Name or Hazard Class:

Sodium Arsenite

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Type of SOP: ☐ Process ☒Hazardous Chemical ☐ Hazardous Class

Purpose

Sodium arsenite is classified by IARC as Group 1, carcinogenic to humans. It is a very toxic chemical when ingested, inhaled, or absorbed through the skin. It may cause irritation to the respiratory tract, gastrointestinal tract, skin, and eyes. Symptoms of arsenic poisoning may occur from prolonged exposure. Sodium arsenite is mainly used as a pesticide. It is also used as a hide preservative, an antiseptic agent, and in soap dyeing.

Physical & Chemical Properties/Definition of Chemical Group

CAS#: 7784-46-5

Class: Carcinogen, very toxic

Molecular Formula: NaAsO2

Form (physical state): Solid, powder

Color: White, grey

Boiling point: Decomposes

Potential Hazards/Toxicity

Sodium arsenite is classified by IARC as Group 1, carcinogenic to humans. It is toxic if inhaled, ingested, and absorbed through the skin. It may cause respiratory tract, skin, and eye irritation. It may cause irritation to the gastrointestinal tract with nausea, vomiting, and diarrhea. Prolonged exposure to arsenic may cause edema, dermatitis, lesions, damage to the nasal septum, hyperpigmentation to the skin, hair and weight loss, excessive salivation and perspiration, central nervous system damage, hepatitis, gastrointestinal disturbances, cardiovascular damage, and kidney and liver damage. May cause skin and lung cancer. Inorganic arsenic-containing compounds have a permissible exposure limit (PEL) of 10 μg/m3.

Engineering Controls

Chemical fume hood. Adequate exhaust and capture filtration.

Personal Protective Equipment (PPE)

Respirator Protection

If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by EH&S. This is a regulatory requirement. (http://www.ehs.ucsf.edu/respiratory-protection-program)

Hand Protection

Handle with gloves. Nitrile gloves are recommended.

NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with sodium arsenite.

Refer to glove selection chart from the links below:

http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf

OR

http://www.allsafetyproducts.biz/page/74172

OR

http://www.showabestglove.com/site/default.aspx

OR

http://www.mapaglove.com/

Eye Protection

ANSI approved, tight-fitting safety glasses/goggles. Face shields are recommended.

Skin and Body Protection

Appropriately-fitting lab coat, long pants, and closed-toe shoes.

Hygiene Measures

Avoid contact with skin, eyes and clothing. Wash hands before breaks and immediately after handling the product.

First Aid Procedures

If inhaled

Move person into fresh air. If not breathing, give artificial respiration. Consult a physician.

In case of skin contact

Wash off with soap and plenty of water for at least 15 minutes while removing contaminated clothing. Take victim immediately to hospital.

In case of eye contact

Flush eyes with plenty of water for at least 15 minutes. Consult a physician.

If swallowed

Do not induce vomiting. Never give anything by mouth to an unconscious person. Rinse mouth with water. Consult a physician.

Special Handling and Storage Requirements

Precautions for safe handling: Avoid contact with skin, eyes, and clothing. Avoid inhalation and ingestion. Avoid dust formation. Provide adequate exhaust ventilation. Open and handle container with care

Conditions for safe storage: Keep container tightly closed in a cool, dry, and well-ventilated area. Air-sensitive. Store under inert gas. Incompatible with strong oxidizing agents and strong acids.

Spill and Accident Procedure

Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-206-8522 (SFGH only)

Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).

Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up.

Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-206-8522 (SFGH only) for assistance.

Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working hours and weekends.

Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for 15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working hours and weekends.

Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone)

Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.

Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-885-7580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday 7:30 a.m. - 4:00 p.m. (except Holidays).

Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.

Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse by dialing 415-353-7842 (STIC).

Decontamination/Waste Disposal Procedure

Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as hazardous waste.

Safety Data Sheet (SDS) Location

Online SDS can be accessed at http://www.ehs.ucsf.edu/safety-data-sheet-sds-1

Protocol/Procedure

Quantities covered by this SOP:

______(g , ml) to ______(g, ml)

Temperature range covered by this SOP:

__ °C – __ °C

General Overview and Purpose:

Enter the experimental purpose

Procedure:

Enter experimental procedure. You can copy procedure from your lab notebook or from literature.

NOTE

Any deviation from this SOP requires approval from the Principal Investigator.

Sodium Arsenite 4 Date: Click here to enter a date.

Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)