CURRICULUM VITAE (2011 07 15)Li Yin, M.D., Ph.D., D.C.H.

Li Yin, M.D., Ph.D., D.C.H.

Research Assistant Professor

Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida

EDUCATION AND QUALIFICATIONS

Doctor of Philosophy (Ph.D.) from Bioengineering Unit, University of Strathclyde, Glasgow, UK (9/92-10/95)

Diploma in Child Health (D.C.H) from Royal College of Physicians and Surgeons of Glasgow, Glasgow, UK (7/90-5/92)

Doctor of Medicine (M.D.) from Beijing Medical University, Beijing, P. R. China (9/80-7/86)

PROFESSIONAL APPOINTMENTS

Research Assistant Professor in the Dept. of Pathology, Immunology and Laboratory Medicine at University of Florida (7/04-presence)

Postdoctoral Associate in Dr. Maureen M. Goodenow’s laboratory in the Dept. of Pathology, Immunology and Laboratory Medicine at University of Florida (8/03-7/04)

Senior Research Scientist and Project Leader in Ixion Biotechnology, Inc (12/00-7/03)

Postdoctoral Research Fellow in Dr. Stewart Sell’s laboratory in University of Texas in Houston, Medical School, Huston, TX, and then, in Albany Medical College, Albany, NY (10/95-12/00)

RESEARCH EXPERIENCE

7/04-presence: Research Assistant Professor in the Dept. of Pathology, Immunology and Laboratory Medicine at University of Florida.

Major research interests are HIV-mediated immune defects, and interaction of HIV-1 with host ecosystem.

  • Mapping molecular defects in IgHV VDJ transcriptome repertoire in HIV-infected young adults by pyrosequencing. Massive parallel pyrosequencing was applied to map the defects in B cell development and antibody (Ab) maturation in HIV-infection. Technical procedures were set up to generate amplicon libraries of isotype unswitched and switched Ig HVVDJtranscriptome repertoiresfor pyrosequencing. Bioinformatic and biostatistic pipelines, including development of a custom germline Ig H VDJ recombination reference sequence database,were established to enable and facilitate studies of somatic hypermutation, Ab biodiversity, VH, DH and JH allelic expression, DH reading frame use, CDR3 length variation, V-D-J recombination, B cell clonality, and Ab sharing among individuals.The established systemprovides a powerful research tool to investigate B cell defects in immune deficiencies, and to evaluate B cell response to vaccination.
  • Long-lived historical record of evolution of viral diversity in peripheral blood cells by deep pyrosequencing.Deep pyrosequencing was applied to investigate the persistence of HIV-1 latent reservoir, HIV-1 biodiversity and population structure, co-receptor bioprofile, and the role of rare variants during the viral evolution within the host ecosystem.
  • The role of Abs against HIV-1 Env gp41 membrane proximal external region (MPER) in mediating mother to child transmission. We hypothesized that women with more broadly neutralizing HIV-1 Abs (bn-Abs-HIV) are less likely to transmit, which could be reflected by increased amino acid polymorphism in HIV-1 Env gp41 MPER where epitopes of known bn-Abs-HIV, including 2F5, 4E10 and Z13, are localized. To test the hypothesis ultra deep pyrosequencing was applied to generate HIV-1 subtype C sequences spanning HR2, MPER and 5’ of MSD regions (318 bp in length) from circulating plasma viruses in 4 mothers who transmitted and 4 mothers who failed to transmit virus to their children by breast feeding. Hot spots within epitopes and diversification of viral population under positive selection were investigated.
  • HPV infection and persistence in external genital lesions of infected men. A web-based HPV genotyping server based on the most updated HPV reference sequences was established to enable and facilitate the HPV genotyping from genital lesions of infected men. The viral population and it’s persistence and change overtime are being investigated using deep sequencing.
  • Immune outcome predicted by dynamics of thymic output and T-cell activation in pediatric HIV infected patients. Thymic functionevaluated by T cell receptor excision circle (TREC) in naïve T cells, and T cell activation measured by T cell receptor Vβ CDR3 length distribution and phenotypic markers were monitored longitudinally after initiation of antiretroviral therapy. Their correlation with immune outcome was studied.
  • Length polymorphism in HIV-1 envelope hypervirable domains V1 and V2 predicts therapy outcome.HIV-1 Env V1/V2 length was closely associated with viral transmission, sensitivity to Ab neutralization and disease progression. In coupled with HIV Env V3 charge, the association of HIV-1 Env V1/V2 length polymorphism with response to antiretroviral therapy was investigated. HIV-1 Env V1/V2 length polymorphism was profiled using spectratyping, and a statistical algorithm was established to analyze the diversity and distribution of V1/V2 length variants within the host and its evolution over time.

8/03-7/04: Postdoctoral Associate in Dr. Maureen Goodenow’s lab in the Dept. of Pathology, Immunology and Laboratory Medicine at University of Florida.

  • Studied HIV-mediated T cell activation by alteration in TCR CDR3 length distribution.

12/00-7/03: Senior Research Scientist and Project Leader in Ixion Biotechnology, Inc., Alachua, FL.

The goal was to establish pancreatic steam cell lines to be able to differentiate into insulin-producing cells for diabetes treatment. To achieve the goal, pancreatic steam cell lines were established, various animal transplantation models were successfully set up, including intra-portal, kidney subcapsular, and intra-pancreatic transplantations, to enable testing the efficacy of in vitro-derived islets from pancreatic stem cells. Q-Med’s non-animal stabilized hyaluronic acid gels (NASHA gels) were evaluated for their scaffolding and immune protective potentials. Liver stem cell lines were also tested for their potentiality to transdifferentiate into insulin-producing cells.

10/95-12/00:Postdoctoral Research Fellow in Dr. Stewart Sell’s laboratory in University of Texas in Houston, Medical School, Houston, TX, and then in Albany Medical College, Albany, NY.

Liver stem cells were proposed to be capable of restoring the mass and function of the liver when the organ is severely injured, and to be responsible for the development of hepatocellular carcinoma (HCC). To test the hypothesis, various animal liver injury models were established to activate liver stem cells and suppress replication of mature hepatocytes, and the roleof liver stem cells, as well as parenchymal and nonparenchymal cells in the injury repair andorgan regeneration was studied. Liver steam cells were isolated, cultured and characterized, and their capability of differentiating into hepatocyte- and bile duct-like cells studied.Role ofp53in controlling development of HCC was investigated.

9/92-10/95:Ph.D. student in Dr. Helen Grant’s laboratory in Bioengineering Unit, University of Strathclyde, Glasgow, UK.

Established immortal hepatocyte cell lines with liver-specific functions to be used for in vitrotesting of drug metabolism and toxicity.

CLINICAL EXPERIENCE

7/90-5/92: Postgraduate Student in Royal College of Physicians and Surgeons of Glasgow, Glasgow, UK. Obtained D.C.H. and accomplished a clinical epidemiological study about dilated cardiomyopathy in infants and children.

9/89-6/90: Chief Resident in the Dept. of Pediatrics in the First School of Beijing Medical University, Beijing, P. R. China.

9/86-7/89: Resident in the Dept. of Pediatrics in the First School of Beijing Medical University, Beijing, P. R. China.

Teaching experience

7/04-presence: Research Assistant Professor in the Dept. of Pathology, Immunology and Laboratory Medicine at University of Florida.

  • A major advisor for a M.S. student (graduated).
  • Train biological scientists to analyze pyrosequences from amplicons of immunoglobulin, HIV-1 and HPV.
  • Supervise students and trainees in study design, experimental design and grant writing, and provided technical support.
  • Present to and advise students and trainees in the HIV Immune Deficiency Journal Club and lab meetings.

12/00-7/03: Senior Research Scientist and Project Leader in Ixion Biotechnology, Inc., Alachua, FL.

  • Supervise and train research assistants in animal operations, including various transplantations and pancreatic islet isolation.

10/95-12/00: Postdoctoral Research Fellow in Dr. Stewart Sell’s laboratory in University of Texas in Houston, Medical School, Houston, TX, and then in Albany Medical College, Albany, NY.

  • Train and superviselab technicians, students and junior postdoctoral research fellows in study design and experimental design, and provide technical supports

9/89-6/90: Chief Resident in the Dept. of Pediatrics in the First School of Beijing Medical University, Beijing, P. R. China.

  • Supervise and teach residents in medical care, diagnosis, treatment and emergency management.

AWARDS

ORS Award and David Livingston Bursary for three years of study leading to the degree of Ph.D. in Bioengineering Unit, University of Strathclyde, Glasgow, UK (9/92-10/95)

Graduate Clinical Studentship in Royal College of Physicians and Surgeons of Glasgow, Glasgow, UK (7/90-5/92)

People’s Scholarship in Beijing Medical University, Beijing, P. R. of China (9/80-7/86)

FUNDINGS

Current Support

R01 DA031017 (Goodenow PI; Yin Co-PI) 09/17/10-06/30/15

NIH/NIDA $4.7 million

Substance use and immunity in HIV+infected adolescents by systems biology.

NIH R01CA098803 (Giuliano PI; Goodenow PI of subcontract; Yin Co-PI of subcontract) 01/15/10-11/30/14

NIH/NCI Total direct cost $73,298

Natural History of HPV Infection in Men (HIM) Study

The overall scope of work for this competitive renewal involves applying high resolution 454 pyrosequencing technology to the study of HPV genotypes in DNA from external genital lesions from subjects enrolled in the HIM Study.

R01 AI47723 04-08 (Sleasman PI; Yin Co-I) 03/01/06 - 02/28/11

NIH/NIAID $2.3 million

Impact of HIV-1 Genotype on Therapy Response in Children

The major focus of the research is to identify genetic determinants in Gag that modulate susceptibility to PI therapy in adolescents who reconstitute immunity without controlling virus replication.

R01 AI06526501 -05 (Goodenow PI; Yin Co-PI) 04/15/05 - 03/31/10

NIH/NIAID $2.2 million

Role of HIV-1 Env Diversity in Cellular Tropism

The major focus of the project is to identify genetic determinants in Env gp120 that confer X4-mediated entry into macrophages.

Past Support

Elizabeth Glaser Pediatric AIDS Foundation (Aldrovandi PI; Yin Co-I) 05/01/08 – 04/30/10

Pediatric HIV Vaccine Program grant $100,000

Human Milk Antibody-Mediated Inhibition of HIV

The goal for the research is to determine the depth of sequence diversity in the membrane proximal external region (MPER) of the HIV-1 Envelope glycoprotein in breast milk samples from infected women and to determine a role for neutralizing antibodies directed at MPER in prevention of HIV-1 transmission to infants by breast milk.

University of Florida Incentive Seed Fund (Goodenow PI; Yin Co-I) 05/01/08 – 04/30/10

University of Florida Office of the VP for Research $90,000

Development of Human Anti-HIV Antibodies as Novel Therapeutics

The long-term goal for the proposed research is to develop novel therapeutics that can prevent infection by a wide variety of clinically relevant strains of HIV-1.

R21 AI078450-01(Goodenow PI; Yin Co-PI) 04/01/08 - 03/31/10

NIH/NIAID $410,000

Characterization of Novel Polyreactive Anti-HIV Antibodies in Autoimmunity

The long-term goal of this proposed research is to develop strategies that lead to induction of broadly reactive neutralizing antibodies that can prevent infection by a wide variety of clinically relevant strains

Florida Center for AIDS Research Incentive Award (Yin PI) 01/12/00 – 12/31/09

$15,000

Development and Application of Bioinformatic Algorithm to Study HIV-1 Env V1/V2 Length Quasispecies and Their Evolution.

The goal/objective is to develop a novel bioinformatic algorithm capable of modeling evolution of HIV-1 Env V1/V2 length diversity to study relationship of HIV-1 Env V1/V2 length quasispecies and their evolution with immune, viral and therapy outcomes.

Florida Center for AIDS Research Incentive Award (Rodriguez PI; Yin Co-PI) 01/12/00 – 12/31/09

$15,000

Defective Immunoglobulin Somatic Hypermutation Results in Pneumococcal Vaccination Hyporesponsiveness in HIV Infected Children.

The goal/objective is to characterize B cell repertoire in HIV infected individuals with poor response to vaccination by investigating mechanisms that lead to hyporesponsiveness to pneumococcal polysaccharide antigens.

UF Cancer Center/Moffitt Collaborative Initiative (UF: Goodenow PI; Yin Co-PI) 01/01/09-12/31/09

$100,000

In-depth Assessment by Pyrosequencing of Prevalence of HPV Genotypes in a Multinational Cohort of HPV-Infected Men.

The goals/objectives are to apply in-depth pyrosequencing to a multi-national cohort of HPV-infected men.

1-R43-DK61781(Yin PI) 05/15/02 - 11/30/03

NIH/SBIR $100,000

Converting Liver Stem Cells into Insulin Producing Cells

The major goalis to investigate the potentiality of liver stem cells to transdifferentiate into insulin-producing cells.

Invited Platform presentations.

  1. Dilated cardiomyopathy in infants and children. Yin, L., and A. Houston. 1992.The Scottish Pediatric Society Summer Meeting. Inverness, Scotland, UK.
  2. Electroporation conditions for optimizing the viability of rat hepatocytes. Yin, L., M. H. Grant, and C. MacDonald. 1994. European Tissue Culture Society-UK Sussex Workshop. Falmer, Brighton, UK.
  3. Pyrosequencing the IgH repertoire. Li Yin. The University of South Florida and University of Florida, Allergy and Immunology Research Retreat, 5/8/10, Children’s Research Institute, St Petersburg, Florida, USA.
  4. Mapping defects in IgM+ B cell repertoire in HIV-infected individuals by 454-pyrosequencing. Yin, L., L. Liu, Y. Cai, W. Hou, M. Wallet, B. Gardner, A. Lowe, Y. Sun, P, Sriaroon, E.E. Perez, C.A. Rodriguez, J.W. Sleasman and M.M. Goodenow. AIDS Vaccine2010, Atlanta, Georgia, USA.
  5. Long-lived historical record of evolution of viral diversity in peripheral blood cells by deep pyrosequencing.Li Yin, Li Liu, Yijun Sun, Wei Hou, Amanda C. Lowe, Marco Salemi, William G. Farmerie, John W. Sleasman, Maureen M. Goodenow. AIDS Vaccine 2011, Bangkok, Thailand.

PUBLICATIONS

Papers

  1. Yin, L., C. MacDonald, and H. Grant. 1996.Electroporation conditions for retention of rat hepatocyte viability. Genetic Engineering Biotechnol. 16:27-34.
  2. Anderson, K., L.Yin, C. MacDonald, and M. H. Grant. 1996. Immortalized hepatocyte as in vitro model systems for toxicity testing: the comparative toxicity of menadione in immortalized cells, primary cultures of hepatocyte and HTC hepatoma cells. Toxicol In Vitro. 10:721-727.
  3. MacDonald C., F. Reid, K. Anderson, L. Yin, E. Hill, and M. H. Grant. 1997. Metabolism and toxicological studies in immortalized rat hepatocyte cell lines, p.73-78. In M. J. T. Carrondo, J. B. Griffiths, and J. L. P. Moreira (ed.), Animal cell technology: from vaccines to genetic medicine. Kluwer Academic Publishers, Dordrecht.
  4. Grant M. H., F. Reid, L. Yin, J. Irvine, K. Anderson, E. Hill, R. Andrews, and C. MacDonald. 1997. Immortalized rat hepatocyte cell lines for in vitro toxicity testing, p.529-533. In K. Funatsu, Y. Shirai, and T. Matsushita (ed.), Animal Cell Technology: Basic and Applied Aspects. Kluwer Academic Publishers, Dordrecht.
  5. Yin, L., N. Ghebranious, S. Chakraboty, C. E. Sheehan, Z. Ilic, and S. Sell. 1998. Control of mouse hepatocyte proliferation and ploidy by p53 and p53ser246 mutation in vivo. Hepatology. 27:73-80.
  6. Anderson, K., R. Andrews, L. Yin, R. McLeod, C. MacDonald, J. D. Hayes, M. H. Grant. 1998. Cytotoxicity of xenobiotics and expression of glutathione-S-transferases in immortalized rat hepatocyte cell lines. Human Exp Toxicol. 17:131-137.
  7. Yin, L., D. Lynch, and S. Sell. 1999. Participation of different cell types in the restitutive response of the rat liver to periportal injury induced by allyl alcohol. J Hepatol. 31: 497-507.
  8. Rosenberg, D, Z. Ilic, L. Yin, and S. Sell. 2000. Proliferation of hepatic lineage cells of normal C57BL and IL-6 knockout mice after cocaine induced periportal injury. Hepatology. 31(4): 948-955.
  9. Sun, M, C. Yang, L. Yin, T. Shupe, Z. Ilic, T. Fridreich, and S. Sell. 2000. Preliminary microarray analysis of differences in mRNA of cell cycle control and carcinogen metabolizing enzymes in normal and p53 null mice. Journal of Tumor Marker Oncology. 15(1):77-84.
  10. Yin, L, D. Lynch, Z. Ilic, and S. Sell. 2002. Proliferation and differentiation of ductular progenitor cells and littoral cells during the regeneration of the rat liver to CCl4/2-AAF injury. Histology and Histopathology. 17(1): 65-81.
  11. Yin, L, M. Sun, Z. Ilic, H. L. Leffert, and S. Sell. 2002. Derivation, characterization, and phenotypic variation of hepatic progenitor cell lines isolated from adult rats. Hepatology. 35(2):315-324.
  12. Peck, A.B., L. Yin and V. Ramiya. 2004. Animal models to study adult stem cell-derivedin vitro-generated islet implantation. Institute For Laboratory Animal Research 45(3):259-267.
  13. Yin L, Rodriguez CA, Hou W, Potter O, Caplan MJ, Goodenow MM, Sleasman JW. 2008. Antiretroviral therapy corrects HIV-1-induced expansion of CD8+ CD45RA+ CD27- CD11abright activated T cells. J Allergy Clin Immunol 122(1):166-172.
  14. Yin, L, Z. C. Kou, C. Rodriquez, W. Hou, M. M. Goodenow, and J. W. Sleasman. 2009. Antiretroviral therapy restores diversity in the T cell receptor V repertoire of CD4 T cell subpopulations among HIV-Infected children and adolescent. Clin Vacc Immunol 16(9):1293-1301.
  15. Wallet MA, Rodriguez CA, Yin L, Saporta S, Chinratanapisit S, Hou W, Sleasman JW, Goodenow MM. 2010. Microbial translocation induces persistent macrophage activation unrelated to HIV-1 levels or T cell activation following therapy. AIDS, 24(9):1281-90.
  16. Yin, L,W. Hou,L. Liu, Y. Cai, Y. Sun, M. A. Wallet, B. P. Gardner, A. C. Lowe, C. A. Rodriguez, W. G. Farmerie,J. W. Sleasman, and M. M. Goodenow. 2011.Molecular defects inIgMHV CDR3 transcriptome repertoire in HIV-infected young adults revealed by pyrosequencing. Submitted.
  17. Yin, L, L. Liu, Y. Sun, W. Hou, M Salemi, W. G. Farmerie, J. W. Sleasman, and M. M. Goodenow. 2011. Long-lived historical record of evolution of viral diversity in peripheral blood cells by deep pyrosequencing. Submitted.
  18. Rodriguez C. A.,L. Yin, W. Hou, S. Ho, A. C. Lowe, M. A. Wallet, P. J. Emmanuel,M. M. Goodenow and J. W. Sleasman. 2011. Dynamics of thymic output, and T-cell activation predict immunologic outcome in pediatric HIV infected patients. In preparation.
  19. Wallet M., L. Yin,J.W. Sleasman, M.M. Goodenow. 2011. Generation of a novel massive-diverse single chain variable fragment antibody phage display library from immunoglobulin genes of lupus patients. In preparation.

Abstracts

  1. Yin, L., and A. Houston. 1992.Dilated cardiomyopathy in infants and children. The Scottish Pediatric Society Summer Meeting. Inverness, Scotland, UK.
  2. Yin, L., M. H. Grant, and C. MacDonald. 1994.Electroporation conditions for optimizing the viability of rat hepatocytes. European Tissue Culture Society-UK Sussex Workshop. Falmer, Brighton, UK. P23.
  3. Yin, L., P. Watts, M. H. Grant. 1994.The effect of hepatocyte growth factor on primary cultures of rat hepatocytes; growth and differentiation. HUG Meeting. Holland.
  4. Yin, L., C. MacDonald, and M. H. Grant. 1995.Electroporation of hepatocytes with retention of viability. The British Toxicology Society Joint BTS/IVTS Meeting. Oxford, UK. P39.
  5. Andrews, R., K. Anderson, L. Yin, C. MacDonald, and M. H. Grant. 1995.Glutathione-S-transferase activities and the toxicity of 1-chloro-2,4-dinitrobenzene and ethacrynic acid in immortalized rat hepatocytes. British Journal of Pharmacology, 116: 343P.
  6. Anderson, K., L. Yin, C. MacDonald, and M. H. Grant. 1995.Testosterone metabolism in freshly isolated cultured and immortalized rat hepatocytes. The British Toxicology Society Joint BTS/IVTS Meeting. Oxford, UK. P38.
  7. Yin, L., C. MacDonald, and M. H. Grant. 1996.Electroporation of hepatocytes with retention of viability. Human & Experimental Toxicology, 15(2): P155.
  8. Anderson, K., L. Yin, C. MacDonald, and M. H. Grant. 1996.Testosterone metabolism in freshly isolated, cultured and immortalized rat hepatocytes. Human & Experimetal Toxicology, 15(2): P154.
  9. Anderson, K., R. Andrews, L. Yin, C. MacDonald, R. McLeod, J. D. Hayes, and M. H. Grant. 1996.Expression of Glutathione-s-transferases in freshly isolated and immortalized rat hepatocytes. Proceedings of the VII Congress of the International Society for Animal Clinical Biochemistry. P90.
  10. MacDonald, C., K. Anderson, L. Yin, E. Hill, and M. H. Grant. 1996.Use of immortalized rat hepatocyte cell lines for metabolism and toxicological studies. 42nd International Congress of the European Tissue Culture Society. Brno, Czech Republic.
  11. MacDonald, C., F. Reid, K. Anderson, L. Yin, E. Hill, and M. H. Grant. 1996.Metabolism and toxicological studies in immortalized rat hepatocyte cell lines. Animal Cell Technology: From Vaccines to Genetic Medicine. HLR-2.
  12. Yin, L., N. Ghebranious, S. Chakraborty, K. Yavokovsky, and S. Sell. 1996.Effect of p53 gene on ploidization and proliferation of mouse hepatocytes. Hepatology 24(4) Pt.2: P258A.
  13. Ilic, Z,. L. Yin, and S. Sell. 1996.Periportal liver “stem” cell proliferation in mouse liver after cocaine-induced injury. Hepatology 24(4) Pt.2: P526A.
  14. Yin, L., D. Lynch, and S. Sell. 1998.Reconstruction of 2AAF-treated rat liver after CCl4-induced pericentral injury by pericentral oval cells. The FASEB Journal. PA468.
  15. Yin, L., D. Lynch, Z. Ilic, and S. Sell. 1998.Participation of different cell types in the restitutive and proliferative response of the rat liver to CCl4 injury after treatment with N-2-acetylaminofluorene (AAF). Hepatology, 28(4) Pt 2: P297A.
  16. Yin, L., Z. Ilic, T. Dion, M. Sun, H. L. Leffert, and S. Sell . 2001.Characterization of liver stem cells derived from ally alcohol-injured rat liver.PAACR 42:354.
  17. Yin, L., M. Sun, Z. Ilic, H. L. Leffert, and S. Sell. 2001.Plasticity of liver progenitor cell lines from allyl alcohol-injured rat liver.FASEB J Late-Breaking Abstracts: 49 (LB267).
  18. Yin, L., M. S. Arenas, A. Clark, E. Jodoin, J. Zhou, H. L. Leffert, S. Sell, A. B. Peck, and V. Ramiya. 2001.The potentiality of liver oval cells to differentiate into insulin-producing cells.Pancreatic Development, Proliferation and Stem Cells meeting, p48.
  19. Yin, L., Z. C. Kou, M. M. Goodenow, and J. W. Sleasman. 2004.HIV infection impacts TCR CDR3 V repertoire in CD4 CD45RA T lymphocytes in Children. 11th conference on retroviruses and opportunistic infections. Abstract No. A-54.
  20. Yin, L., Z. C. Kou, M. M. Goodenow, and J. W. Sleasman. 2005.Comparison of TCR CDR3 length distribution in CD4 and CD8 T cell Naive and memory subpopulations in healthy and HIV-infected children before and after antiretroviral therapy (ART). 12th conference on retroviruses and opportunistic infections. Abstract No. 111.
  21. Rodriguez C, Yin L, Koch S, Ghaffari G, Haraguchi S, Lowe A, Goodenow M and Sleasman J. 2006.Preservation of CD4 T cell production Despite High HIV replication in children and adolescents receiving PI-containing ART. 13rd conference on retroviruses and opportunistic infections. Abstract #: R-134.
  22. Li Yin, S. McCready, A. Lowe, J. Oshier, J. W. Sleasman and M. M. Goodenow. 2007.HIV-1 Env V1V2 Length Polymorphism Predicts Combination Antiretroviral Therapy Outcome in HIV-Infected Children. 14thd conference on retroviruses and opportunistic infections. Abstract # R-174.
  23. Rodriguez, C, L. Yin, M. Morrow, P. Emmanuel1, M. M. Goodenow and J. W. Sleasman.2007.Sustained Increased Thymic Output and Decreased Immune Activation in Children and Adolescents with High Post Therapy Viral Loads while Receiving Protease Inhibitor Containing ART. 14thd conference on retroviruses and opportunistic infections. Abstract #: R-121.
  24. Li Yin, Li Liu, Yijun Sun, Rebecca Gray, Amanda C. Lowe, Wei Hou, John W. Sleasman and Maureen M. Goodenow. Ultra-deep Pyrosequencing Captured Low Frequency CXCR4 Virus Populations Co-archived with CCR5 Virus in Peripheral Blood Lymphocytes from HIV-infected Therapy-naïve Children. 2010. 17th Conference on Retroviruses and Opportunistic Infections. Abstract #275, P160.
  25. Yin, L., L. Liu, Y. Cai, W. Hou, M. Wallet, B. Gardner, A. Lowe, Y. Sun, P, Sriaroon, E.E. Perez, C.A. Rodriguez, J.W. Sleasman and M.M. Goodenow. 2010. Mapping defects in IgM+ B cell repertoire in HIV-infected individuals by 454-pyrosequencing. AIDS Vaccine 2010. Oral presentation. ARHR OA09.08LB, A-149.
  26. Li Yin, Li Liu, Wei Hou, Yunpeng Cai, Mark. A. Wallet, Brent P. Gardner, Amanda C. Lowe, Carina A. Rodriguez, John W. Sleasman and Maureen M. Goodenow. 2011. Prolonged suppressive antiretroviral therapy failed to correct HIV-1-mediated defects innaïve and IgM+ memory B cell transcriptome repertoires.18thd conference on retroviruses and opportunistic infections. Abstract #: D-200.
  27. Li Yin, Li Liu, Yijun Sun, Wei Hou, Amanda C. Lowe, Marco Salemi, William G. Farmerie, John W. Sleasman, Maureen M. Goodenow. 2011.Long-lived historical record of evolution of viral diversity in peripheral blood cells by deep pyrosequencing. AIDS Vaccine 2011. Oral presentation. ARHR.
  28. Li Yin, Li Liu, Wei Hou, Yunpeng Cai, Mark A. Wallet, Brent P. Gardner, Amanda C. Lowe, Carina A. Rodriguez, John W. Sleasman and Maureen M. Goodenow.2011. Molecular Defects inIgMVH CDR3 Transcriptome Repertoire in HIV-infected and Systemic Lupus Erythematosus Young Adults Revealed by Pyrosequencing. University of Florida College of Medicine Research Day, Gainesville, FL, USA.

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