L. Youssar and J. Avalos: Genetic basis of the ovc phenotype of Neurospora: identification and analysis of a 77 kb deletion

supplementaL DATA 1

Predicted genes deleted in the ovc mutant

ORF #a / ORF sizeb / Predicted functionc / Best match (accession number)d / e valuee
07 / 379 / transmethylase / Plant phosphoethanolamine N–methyltransferase (Q9M571) / 2e07
08 / 542 / hydrolase / Hypothetical bacterial metal-dependent hydrolase (Q9X0P5) / 6e-06
09 / 452 / transmethylase / Putative plant phosphoethanolamine N–methyltransferase (Q944H0) / 1e-03
10 / 365 / transmethylase / Plant phosphoethanolamine N–methyltransferase (Q9FR44) / 4e-09
11 / 784 / Unknown / None
12 / 607 / Vegetative incompatibility protein / P. anserina vegetatible incompatibility protein HET-E-1 (Q00808) / 7e-50
13 / 149 / Unknown / Hypothetical bacterial UPF0135 type protein (Q9KD39) / 4e-12
14 / 333 / Unknown / None
15 / 283 / Unknown / None
16 / 398 / Unknown / ORF #19 / 7e-31 *
17 / 369 / transmethylase / Putative plant phosphoethanolamine N-methyltransferase (Q944H0) / 4e-05
18 / 662 / Unknown / ORF #19 * / 6e-26 *
19 / 650 / Unknown / ORF #16 * / 4e-27 *
20 / 766 / Unknown / None
21 / 420 / Unknown / P. nodorum hypothetical protein (EAT83762) * / 2e-90 *
22 / 69 / Unknown / None
23 / 331 / Reductase / S. pombe probable oxidoreductase (Q10494) / 4e-55
24 / 526 / Phosphatase (CUT-1, Youssar et al. 2005) / Hypothetical S. cerevisiae protein (P36151) / 3e-60
25 / 134 / Unknown / None
26 / 174 / Unknown / None
27 / 688 / Unknown / M. grisea hypothetical protein (XM359831) * / 4e-91 *

agene number from the code NCU049XX.1

b ORF size (aa number) as deduced from the annotated gene in the Neurospora genome database (

c Putative function deduced from sequence comparison

dBest BlastP match against SwissProt data base. If best match was not significant (evalue under 1e03), best match against translated nucleotide data bases (GenBank+EMBL+DDBJ+PDB) is shown instead and indicated with an asterisk

e e-value for the best match, shown at the left column. Only e-values under 1e03 were considered.

Cited reference:

Youssar L, Schmidhauser TJ, Avalos J (2005) The Neurospora crassa gene responsible for the cut and ovc phenotypes encodes a protein of the haloacid dehalogenase family. Mol Microbiol 55:828-838