Population genetic structure and sex-biased dispersal of the hazel dormouse (Muscardinusavellanarius)in a continuous and in a fragmented landscape incentral Italy

Bani L1, Orioli V1, Pisa G1, Fagiani S1, Dondina O1, Fabbri E2, Randi E2,3, Sozio G4,Mortelliti A5

Supplementary material 1:

Supplementary Tables

Table S1.1. Number of genotyped adult individuals per sampling location (SL), splitted for sex and trapping session.

Sampling population / Sampling location (SL) / Trapping session
1 / Trapping session
2 / Trapping session
3 / Total number of individuals
Males / Females / Males / Females / Males / Females
SLR / 11 / 6 / 7 / 18 / 10 / 5 / 2 / 48
110 / - / - / 4 / 7 / 1 / - / 12
12 / 1 / 2 / 6 / 6 / - / 1 / 16
17 / 5 / 8 / 15 / 10 / 1 / 1 / 40
19 / - / - / 2 / 3 / 4 / 1 / 10
VTH / 21 / - / - / 4 / 5 / - / - / 9
23 / - / - / 4 / 8 / 8 / 9 / 29
25 / - / 1 / 2 / 4 / 6 / 2 / 15
26 / - / 4 / 5 / 2 / 4 / 2 / 17
27 / 1 / 2 / - / 2 / - / - / 5
28 / 1 / 1 / 5 / 5 / - / - / 12

Table S1.2. Hazel Dormouse species-specific microsatellite markers (Alice Mouton, personal communication, modified from Naim et al. 2009).

Locus / Primer sequence (5’ → 3’) / Label / Ta (°C) / Reference
Mav1 / F: ATAGCCCAGAGGTAGAAA / HEX / (TD) 55 °C / Mouton modified from Naimet al. 2009
R: TAGCATCCCGTTCTCAAACC
Mav2 / F: GAAGGGCTGGGTATATAT / FAM / (TD) 63 °C / Mouton modified from Naimet al. 2009
R: GCCTCCACCCAGAGACCG
Mav3 / F: CACATGTGTTGACTGATT / HEX / (TD) 60 °C / Mouton modified from Naimet al. 2009
R: TCATAGGTTTGCGTCCAGCC
Mav4 / F: GTAGAGCTGAGGGTATAACTTGG / FAM / (TD) 60 °C / Mouton modified from Naimet al. 2009
R: GCTGGTATTGCATGAGACG
Mav5 / F: CCATTGGTCCAAGCCACA / HEX / (TD) 60 °C / Mouton modified from Naimet al. 2009
R: GAAAGAACCTCAATTAAAGC
Mav6 / F: TCTTGCCTCGAAATGACT / FAM / (TD) 55 °C / Mouton modified from Naimet al. 2009
R: AGGTGTAAGGGTATAGCTTGG
Mav7 / F: AAGTTGCTTGGTCTCTTTGG / HEX / (TD) 63 °C / Mouton modified from Naimet al. 2009
R: GCTAAGACTCAAACCCAAGGC
Mav8 / F: GTGTAGCTTGAAGGTAGA / FAM / (TD) 63 °C / Mouton modified from Naimet al. 2009
R: AAACTTGGTACTAGTGCAGAC

Table S1.3. Pairwise Genetic distances (Fst) between sampling locations (SLs).

SL11 / SL12 / SL17 / SL19 / SL110 / SL21 / SL23 / SL25 / SL26 / SL27
SL12 / 0.0354
SL17 / 0.0192 / 0.0187
SL19 / 0.0255 / 0.0117 / 0.0070
SL110 / 0.0366 / 0.0543 / 0.0297 / 0.0326
SL21 / 0.0957 / 0.1038 / 0.0738 / 0.0941 / 0.0823
SL23 / 0.1019 / 0.1079 / 0.0977 / 0.0865 / 0.0958 / 0.1094
SL25 / 0.1547 / 0.1908 / 0.1700 / 0.2039 / 0.1588 / 0.1406 / 0.1557
SL26 / 0.1384 / 0.1507 / 0.1453 / 0.1783 / 0.1524 / 0.1495 / 0.1896 / 0.1531
SL27 / 0.1259 / 0.1579 / 0.1174 / 0.1556 / 0.1109 / 0.1372 / 0.0810 / 0.1024 / 0.2001
SL28 / 0.1258 / 0.1620 / 0.1421 / 0.1750 / 0.1478 / 0.1897 / 0.1082 / 0.1700 / 0.1403 / 0.1241