Electronic supplemental materials to

Molecular phylogeny of tribe Forsythieae (Oleaceae) based on nuclear ribosomal DNA internal transcribed spacers and plastid DNA trnL-F and matK gene sequences


Table S1 Vouchers and GenBank accession numbers for the sequenced taxa.

Taxa / Source of DNA/Voucher / GenBank accession number
ITS / trnL-F / matK
Oleaceae
Tribe Forsythieae
Abeliophyllum distichum Nakai
Abeliophyllum distichum for. lilacinum Nakai
Abeliophyllum distichum for. eburneum T. Lee
Forsythia koreana Nakai
Forsythia saxatilis Nakai
Forsythia ovata Nakai
Forsythia nakaii T.Lee
Forsythia japonica Makino
Forsythia viridissima Lindley
Forsythia suspensa Vahl
Forsythia Í intermedia Zabel
Forsythia giraldiana Ling
Forsythia europaea Degen & Bald.
Tribe Fontanesieae
Fontanesia fortunei Carriere
Fontanesia phillyreoides Labill.
Tribe Jasmineae
Jasminum nudiflorum Lindley
Jasminum humile L.
Tribe Oleeae
Chionanthus retusus Lindl. & Paxton
Fraxinus rhynchophylla Hance
Ligustrum obtusifolium Siebold & Zucc.
Osmanthus heterophyllus (G.Don) P.S.Green
Syringa oblata var. dilatata (Nakai) Rehder
Gesneriaceae
Streptocarpus caulescens Vatke
Scrophulariaceae
Myoporum insulare R.Br
Myoporum montanum R.Br
Verbenaceae
Verbena rigida Spreng.
Bignoniaceae
Campsis radicans (L.) Seem. ex Bureau
Paulowniaceae
Paulownia tomentosa (Thunb.) Steud.
Acanthaceae
Avicennia marina (Forssk.) Vierh. / Kim & Jeon 0044, Korea
Kim & Jeon 0027, Korea
Kim & Jeon 0010, Korea
Kim & Jeon 0144, Korea
Kim & Jeon 0048, Korea
Kim & Jeon 0100, Korea
Kim & Jeon 0154, Korea
M. Togashi 130, Japan
Kim & Jeon 0033, Korea
RBGKew 264-79, 06640
RBGKew 000-73, 21134
RBGKew 1995-1652, 1137
RBGKew 367-32, 36906
Kim s. n., Korea
Kim & Jeon 0156, Korea
Kim & Jeon 0018, Korea
RBGKew s. n.
Kim s. n., Korea
Kim s. n., Korea
Kim s. n., Korea
Kim s. n., Korea
Kim s. n., Korea
-
-
-
-
-
- / AF534803
AF534804
AF534805
AF534806
AF534807
AF534812
AF534811
AF534813
AF534810
AF534808
AF540073
AF534809
AF534814
AF534815
AF534816
AF534817
AF534818
HM751201
HM751202
HM751203
HM751204
HM751205
AF316920
EU886845
FJ867403
DQ515201
AF478941
AF477771 / EU281150
EU281151
EU281152
EU281153
EU281154
EU281155
EU281156
EU281144
EU281145
EU281157
EU281158
EU281159
EU281160
EU281148
EU281149
EU281146
EU281147
EU281165
EU281162
EU281161
EU281164
EU281163
AJ430918
AJ430934
AJ430940
FJ870022
AJ430926
AJ430913 / EU281166
EU281168
EU281167
EU281169
EU281170
EU281171
EU281172
EU281173
EU281174
EU281175
EU281176
EU281177
EU281178
EU281179
EU281180
EU281181
EU281182
HM751206
HM751207
HM751208
HM751209
HM7512010
AJ429331
AF531808
AJ429353
AF531775
AF051997
AB114527


Table S2 Primer sequences used for PCR and sequencing.

Name / Primer sequence (5′→3′) / Position / Reference
ITS5
ITS4
trn1c
trn2d
trn5e
trn6f
3914F
–19F
1412F
900R
1470R
2R / GGAAGTAAAAGTCGTAACAAGG
TCCTCCGCTTATTGATATGC
CGAAATCGGTAGACGCTACG
GGGGATAGAGGGACTTGAAC
GGTTCAAGTCCCTCTATCCC
ATTTGAACTGGTGACACGAG
GGGGTTGCTAACTCAACGG
CGTTCTGACCATATTGCACTATG
ATATAATTCTTATGTATGTG
GAAGCCAGAATTGCTTTTCCT
AAGATGTTGATYGTAAATGA
AACTAGTCGGATGGAGTAG / ITS fwd
ITS rev
trnL fwd
trnL rev
trnL-F igs fwd
trnL-F igs rev
5′-trnK intron fwd
5′-trnK intron fwd
matK internal fwd
matK internal rev
matK internal rev
3′-trnK intron rev / White et al. (1990)
White et al. (1990)
Taberlet et al. (1991)
Taberlet et al. (1991)
Taberlet et al. (1991)
Taberlet et al. (1991)
Johnson & Soltis (1995)
Gravendeel et al. (2001)
Johnson & Soltis (1995)
Johnson & Soltis (1995)
Johnson & Soltis (1995)
Steele & Vilgalys (1994)


Table S3 Summary descriptions and parsimony analysis results for sequences included in individual and combined analyses of nuclear ribosomal region (ITS) and two plastid regions (trnL-F and matK).

Characters / ITS (I) / trnL-F (II) / matK (III) / Plastid
(II + III) / Combined
(I + II + III)
Length of sequence region (bp)
Abeliophyllum
Forsythia
Fontanesia
Jasminum
Aligned length
Parsimony informative sites
Variable sites
Constant sites
G + C (%)
Abeliophyllum
Forsythia
Fontanesia
Jasminum
Sequence divergence
Abeliophyllum
Forsythia
Fontanesia
Jasminum
Abeliophyllum vs Forsythia
Abeliophyllum vs Fontanesia
Fontanesia vs Jasminum / 623
624–626
623
607-620
721
248
354
367
55.2
55.2
60.8
60.8
0.004-0.008
0.004-0.069
0.018
0.015
0.08-0.11
0.156-0.17
0.008-0.021 / 890
890–892
890–893
886-912
1006
93
272
734
35.4
35.4
34.9
34.8
0.001-0.002
0.001
0.005
0.038
0.001-0.004
0.018-0.021
0.045-0.06 / 1518
1519–1522
1524–1525
1524
1540
163
426
1114
38.3
38.3
39.7
33.2
0
0.001-0.01
0.005
0.007
0.002-0.007
0.017-0.022
0.021-0.026 / 2408
2409-2414
2414-2418
2410-2436
2546
256
698
1848
33.8
33.9
34.2
33.8
0-0.001
0.001-0.007
0.005
0.019
0.002-0.006
0.017-0.022
0.03-0.038 / 3031
3033–3037
3038–3040
3030-3043
3267
504
1052
2215
38.2
38.3
39.7
39.3
0.001-0.002
0.001-0.017
0.006
0.018
0.018-0.025
0.043-0.049
0.026-0.034