S2. Sequence specific DNA markers scored in population 97-30.

Ch / Marker / Reference or primer sequence 5’→3’ / Ta (ºC) / PCR
product size (bp) / Type of
polymorphism a / Restriction
enzyme
IV / BA106 c14 t7 / Paal et al. 2004 / 56 / 500 / SSCP / MseI
V / BA100 e13 A/B / A: gtc ctt gtc ttg ctt agc aga taa g, B: gcc aaa aag aga ctc aat gtg atc / 55 / 500 / SCAR / -
BA151 m8 C/D / C: gga tta agg agg ata ttc gac agc, D: atc cat gtc atc tct ttc atg tta / 55 / 400 / SCAR / -
VIII / 573 / Bormann et al. 2004 / 58 / 800 / ASA / -
BA73 e8 t3 / F: aga cca aat aaa ggt ata tga aga tc, R: gtt aat atc act cat ggc act atg / 56 / 350 / CAPS / AluI
IX / GP39 / Marczewski et al. 2006 / 51 / 950 / SSCP / MseI
B7 / F: ggg cct agt tca atg tac, R: agt tgc tat ggt tgt tcc / 54 / 600 / SSCP / MseI
GP94 / F: atg tat cac aat cac att ctt gct c, R: tgt aaa acc aac aag tag tgt tgc / 56 / 300 / SCAR / -
GP129 / F: gtg gta gca aag tat tca tc, R: cgt tat ctg gac tcc ttt ag / 56 / 450 / SSCP / DdeI
CP135 / F: gaa aac taa ggt ggc cag aac, R: gga aga gta cat gga tac att g / 56 / 250 / SSCP / MseI
S1D11 / F: gtt gta att tct tca gca aaa tta gc, R: ttc acg aat cgg act aaa aga ttg / 56 / 250 / SSCP / -
X / CP105 / F: gat gtt gta caa gct tgt caa acc, R: caa aat cag gcc att gtg aat gag / 56 / 400 / CAPS / AluI

a SSCP = single strand conformation polymorphism, CAPS = cleaved amplified polymorphic sequence, SCAR = sequence characterized amplified region, ASA = allele specific amplification.

Bormann CA, Rickert AM, Castillo Ruiz RA, Paal J, Lübeck J, Strahwald J, Buhr K, Gebhardt C (2004) Tagging quantitative trait loci for maturity-corrected late blight resistance in tetraploid potato with PCR-based candidate gene markers. Mol Plant-Microbe Interact 17: 1126-1138

Marczewski W, Strzelczyk-Żyta D, Hennig J, Witek K, Gebhardt C (2006) Potato chromosomes IX and XI carry genes for resistance to potato virus M. Theor Appl Genet 112: 1232-1238

Paal J, Henselewski H, Muth J, Meksem K, Menéndez CM, Salamini F, Ballvora A, Gebhardt C (2004) Molecular cloning of the potato Gro1-4 gene conferring resistance to pathotype Ro1 of the root cyst nematode Globoderarostochiensis, based on a candidate gene approach. Plant J 38: 285-297