QUICK REFERENCE PHLEBOTOMY GUIDE

For Laboratory Blood Collections

I. By tube type/color: Chemistry and Immunochemistry

A.Yellow With Gel Top Tubes: B. Plain Red Top:

·  Basic/Comprehensive Panels Thyroid Tests

·  Blood Alcohol * Therapeutic Drug Levels:

·  BUN/Creatinine Dilantin, Digoxin,

·  Electrolytes Phenobarb., Tegetol

·  Glucose Vanco., Tobramycin

·  CPK C. Large Purple Top Tube:

·  HCG *BNP

·  PSA D. Gray Top Tubes:

·  Intact PTH *Lactate (on ice)

E.  Green top with gel

CPKMB/Troponin F. Green Top Tube:

G. Lavender Top Tube: *Ammonia (on ice)

Hemoglobin A1c/Glycohcg

RBC Folate

II. By tube type/color: Hematology

A. Lavender B. Blue*must fill tubes

§  CBC *Prothrombin time (PT)

§  CBC with Diff *PTT

§  Platelet Count *Unfractionated Heparin

§  Hemoglobin/Hematocrit *Low Molecular Weight

§  Retic Heparin (LMWH)

* Fibrinogen

§  Sed Rate * D-Dimer

§  Pediatric Blood Bank PFA call Hem @28147

*Bleeding Time Call Hem

PLAVIX (special tube)

Call Hemo 28147

III. Blood Bank/Transfusion Services

Pink Top , 6ml EDTA tube

Optional tubes, both lavender and red

IV.  Microbiology/Blood Cultures

Ø  Blue and Purple bottles is a set: 5-10mls/bottle

Ø  Yellow, pediatric/low volume: 1-4mls

Ø  Blue, fungal blood culture: 5-10mls/bottle

Ø  Black, AFB blood culture, call Micro 2-8152

V. Laboratory Collection Guide in Alpha Order

Must identify both patient and specimen following red rules

Test / Tube Type / Special Notes / Dept.
Ammonia / Green / Transport on ice / Chemistry
Basic/Comprehensive Panels / Yellow / Chemistry
Blood bank testing / Pink 6ml / Collector and Witness required for collection / Blood Bank
Blood Cultures,routine / Blue/
Purple / 5-10 mls/bottle
Must collect aseptically / Microbiology
*call for afb
to 2-8152
Blood Cultures, pedi / Yellow / 1-4 mls /bottle / microbiology
B-NP / Purple / 6ml tube / Chemistry
CBC with Diff / Lavender / Hematology
CBC / Lavender / Hematology
CPK / Yellow / Chemistry
CPKMB/Troponin / Green with gel / Chemistry
D-Dimer / Blue / Tube must be full / Hematology
Electrolytes / Yellow / Chemistry
Fibrinogen / Blue / Tube must be full / Hematology
Glucose / Yellow / Chemistry
HCG / Yellow / Chemistry
Hemoglobin/Hematocrit / Lavender / Hematolgy
Hemoglobin A1c / Lavender / Chemistry
Heparin,low molecular weight (LMWH) / Blue / Tube must be full / Hematology
Heparin, Unfractionated / Blue / Tube must be full / Hematology
Intact PTH / Yellow / Chemistry
Lactate / Gray / Transport on ice / Chemistry
Platelet Count / Lavender / Hematology
Plavix / Special tube / Call hematology
28147 / Hematology
Prothrombin Time (PT) / Blue / Tube must be full / Hematology
PSA / Yellow / Chemistry
PTT / Blue / Tube must be full / Hematology
Retic / Lavender / Hematology
Sed Rate/ESR / Lavender or Black / Tube must be full / Hematology
Thyroid Tests / Red / Chemistry

Bleeding Time and PFA, Blue Top, call Hematology at 2-8147

Order of draw for test tubes

Vacutainer tube methode Syringe and safety transfer device

White (discard) Blue

Blue Lavender or Pink

Gold or Red Gold or Red

Lavender or Pink

Blood cultures are ALWAYS drawn first