Movie 1. Untreated Jurkat

Movie Legends.

Movie 1. Untreated Jurkat.

The type II cell line, Jurkat was fixed in PBS/PFA. CD95 was stained with APO1-3 and revealed with the secondary Alexa555-coupled Goat anti mouse mAb (red) and lipids rafts were stained using a FITC-conjugated cholera toxin subunit B (CTB) (green). Nuclei were stained with DAPI (blue). Superimposed staining of CD95 with lipid rafts is depicted by yellow color.

Movie 2. CD95L-treated Jurkat.

Jurkat was treated with FLAG-CD95L (1 µg/ml) at 4°C for 30 minutes and then washed. Cells were next incubated at 37°C for 15 minutes, fixed in PBS/PFA. CD95 was stained with APO1-3 and revealed with the secondary Alexa555-coupled Goat anti mouse mAb (red) and lipids rafts were stained using a FITC-conjugated cholera toxin subunit B (CTB) (green). Nuclei were stained with DAPI (blue).

Movie 3.APO1-3-treated Jurkat.

Jurkat was treated with APO1-3 (1 µg/ml) at 4°C for 30 minutes and then washed. Cells were next incubated at 37°C for 15 minutes, fixed in PBS/PFA. CD95 was revealed with the secondary Alexa555-coupled Goat anti mouse mAb (red) and lipids rafts were stained using a FITC-conjugated cholera toxin subunit B (CTB) (green). Nuclei were stained with DAPI (blue).

Movie 4.Untreated H9.

The type I cell line, H9 was fixed in PBS/PFA. CD95 was stained with APO1-3 and revealed with the secondary Alexa555-coupled Goat anti mouse mAb (red) and lipids rafts were stained using a FITC-conjugated cholera toxin subunit B (CTB) (green). Nuclei were stained with DAPI (blue). Superimposed staining of CD95 with lipid rafts is depicted by yellow color.

Movie 5.CD95L-treated H9.

H9 was treated with FLAG-CD95L (1 µg/ml) at 4°C for 30 minutes and then washed. Cells were next incubated at 37°C for 15 minutes, fixed in PBS/PFA. CD95 was stained with APO1-3 and revealed with the secondary Alexa555-coupled Goat anti mouse mAb (red) and lipids rafts were stained using a FITC-conjugated cholera toxin subunit B (CTB) (green). Nuclei were stained with DAPI (blue).

Movie 6.APO1-3-treated H9.

H9 was treated with APO1-3 (1 µg/ml) at 4°C for 30 minutes and then washed. Cells were next incubated at 37°C for 15 minutes, fixed in PBS/PFA. CD95 was revealed with the secondary Alexa555-coupled Goat anti mouse mAb (red) and lipids rafts were stained using a FITC-conjugated cholera toxin subunit B (CTB) (green). Nuclei were stained with DAPI (blue).