Supplemental Table 1: Results of Site Specific KA/KS Analysis

Supplemental Table 1: Results of Site specific KA/KS analysis.

Model1 / p2 / l3 / k4 / Estimates of Parameters5 / LRT6 / Positively Selected Sites7
OR Family A
M0 / 19 / -4482.74 / 2.07 / w = 0.3540 / N/A / None
M1 / 19 / -4424.79 / 2.27 / p0 = 0.442(p1 = 0.558) / N/A / Not allowed
M2 / 21 / -4400.96 / 2.05 / p0 = 0.284, p1 = 0.293,
(p2 = 0.423), w2 = 0.220 / 47.66 P<0.001 / None
M3 / 23 / -4398.44 / 2.08 / p0 = 0.534, p1 = 0.389,
(p2 = 0.076), w0 = 0.055,
w1 = 0.626, (w2 = 1.826) / 168.60 P<0.001 / >0.95: T142 A255
>0.50: T32 T47 L86 L91 A99 I109 S164 K167 L211 R227 R234 T258 T303
M7 / 20 / -4402.34 / 2.03 / p = 0.268, q = 0.453 / N/A / Not allowed
M8 / 22 / -4398.84 / 2.09 / p0 = 0.943, (p1 = 0.057),
p = 0.351, q = 0.744,
w = 1.931 / 7.00 P<0.05 / >0.95: A255
>0.50: T32 T47 L86 L91 T142 S164 L211 T303
OR Family B
M0 / 19 / -3672.44 / 2.6 / w = 0.4267 / N/A / None
M1 / 19 / -3629.84 / 2.71 / p0 = 0.489(p1 = 0.511) / N/A / Not allowed
M2 / 21 / -3621.44 / 2.78 / p0 = 0.482, p1 = 0.504,
(p2 = 0.014), w2 = 6.655 / 16.80
P<0.001 / >0.99: R308
>0.95: R88
>0.50: I256
M3 / 23 / -3614.66 / 2.67 / p0 = 0.348, p1 = 0.555,
(p2 = 0.097), w0 = 0.000,
w1 = 0.458, (w2 = 2.384) / 115.56
P<0.001 / >0.99: R88 I256 R308
>0.95: W106 A200 I206
>0.50: M3 E4 S10 H21 Q27 D83 C137 S153 L160 Y193 I196 M197 D201 V205 V215 F257 V276 S299
M7 / 20 / -3624.7 / 2.58 / p = 0.190, q = 0.275 / N/A / Not allowed
M8 / 22 / -3614.99 / 2.68 / p0 = 0.936, (p1 = 0.064),
p = 0.405, q = 0.813,
w = 2.764 / 19.42
P<0.001 / >0.99: R308
>0.95: R88 I256
>0.50: M3 Q27 D83 W106 C137 S153 M197 A200 D201 V205 I206 S299
OR Family C
M0 / 19 / -3270.72 / 3.54 / w = 0.1979 / N/A / None
M1 / 19 / -3220.92 / 3.69 / p0 = 0.690(p1 = 0.310) / N/A / Not allowed
M2 / 21 / -3212.06 / 3.51 / p0 = 0.601, p1 = 0.144,
(p2 = 0.255), w2 = 0.302 / 17.72 P<0.001 / None
M3 / 23 / -3209.9 / 3.56 / p0 = 0.623, p1 = 0.334,
(p2 = 0.043), w0 = 0.000,
w1 = 0.461, (w2 = 2.110) / 121.64 P<0.001 / >0.99: D18
>0.95: I32
>0.50: I24 L68 A104 C124 V150 L197
M7 / 20 / -3212.77 / 3.51 / p = 0.111, q = 0.385 / N/A / Not allowed
M8 / 22 / -3210.74 / 3.56 / p0 = 0.975, (p1 = 0.025),
p = 0.154, q = 0.657,
w = 2.424 / 4.06 P=0.131 / >0.50: D18 I24 I32 L68 A104 C124
OR Family D
M0 / 25 / -4657.74 / 2.96 / w = 0.2847 / N/A / None
M1 / 25 / -4600.93 / 3.19 / p0 = 0.531(p1 = 0.469) / N/A / Not allowed
M2 / 27 / -4566.24 / 2.89 / p0 = 0.378, p1 = 0.192,
(p2 = 0.430), w2 = 0.230 / 69.38 P<0.001 / None
M3 / 29 / -4562.85 / 2.96 / p0 = 0.419, p1 = 0.476,
(p2 = 0.106), w0 = 0.000,
w1 = 0.334, (w2 = 1.616) / 189.78 P<0.001 / >0.99: M26 A105 T139 H220
>0.95: G25 A33 Q134 I153
>0.50: R2 R3 R20 A21 T95 I106 A108 D109 V147 H165 E196 V201 V205 D269 V276 R295 G299 R302
M7 / 26 / -4569.83 / 2.89 / p = 0.233, q = 0.562 / N/A / Not allowed
M8 / 28 / -4563.72 / 2.96 / p0 = 0.924, (p1 = 0.076),
p = 0.388, q = 1.430,
w = 1.785 / 12.22 P<0.01 / >0.95: M26 A105
>0.50: R2 R3 R20 A21 G25 A33 T95 A108 D109 Q134 T139 V147 I153 H165 E196 V201 V205 H220 R295 R302
OR Family E
M0 / 31 / -6072.33 / 2.58 / w = 0.3084 / N/A / None
M1 / 31 / -5968.57 / 2.9 / p0 = 0.456(p1 = 0.544) / N/A / Not allowed
M2 / 33 / -5961.01 / 3.04 / p0 = 0.453, p1 = 0.501,
(p2 = 0.046), w2 = 3.051 / 15.12 P<0.001 / >0.95: D5
>0.50: A90 E91 R92 T112 R142 Y162 I164 L165 K177 R233 T269 Y312
M3 / 35 / -5902.31 / 2.69 / p0 = 0.462, p1 = 0.404,
(p2 = 0.133), w0 = 0.020,
w1 = 0.396, (w2 = 1.531) / 340.04 P<0.001 / >0.99: D5 A90 R92 Y162 I164 L165 R233 T269 Y312
>0.95: N6 V86 E91 G101 T112 R142 C161 K177
>0.50: P24 R27 L74 I76 L98 S105 S106 L171 M189 S199 V201 S209 T213 I259 I260 G305 K309 Q310
M7 / 32 / -5911.56 / 2.58 / p = 0.247, q = 0.513 / N/A / Not allowed
M8 / 34 / -5902.58 / 2.69 / p0 = 0.904, (p1 = 0.096),
p = 0.364, q = 1.163,
w = 1.692 / 17.96 P<0.001 / >0.95: D5 Y312
>0.50: N6 R27 V86 A90 E91 R92 G101 S105 T112 R142 C161 Y162 I164 L165 K177 M189 V201 S209 T213 R233 T269 G305 Q310
Human Testis OR orthologs
M0 / 71 / -11885.43 / 2.49 / w = 0.2875 / N/A / None
M1 / 71 / -11875.59 / 3.06 / p0 = 0.282(p1 = 0.718) / N/A / Not allowed
M2 / 73 / -11820.22 / 3.1 / p0 = 0.282, p1 = 0.712,
(p2 = 0.007), w2 = 12.907 / 110.74
P<0.001 / >0.99: P18 Q47
M3 / 75 / -11532.54 / 2.51 / p0 = 0.589, p1 = 0.404,
(p2 = 0.007), w0 = 0.076,
w1 = 0.649, (w2 = 7.083) / 705.78
P<0.001 / >0.99: P18 Q47
M7 / 72 / -11549.51 / 2.48 / p = 0.402, q = 0.846 / N/A / Not allowed
M8 / 74 / -11511.5 / 2.52 / p0 = 0.993, (p1 = 0.007),
p = 0.426, q = 0.933,
w = 7.012 / 76.02
P<0.001 / >0.99: P18 Q47
Human Testis OR orthologs: Rat
M0 / 45 / -8365.45 / 2.41 / w = 0.2962 / N/A / None
M1 / 45 / -8340.91 / 2.93 / p0 = 0.312(p1 = 0.688) / N/A / Not allowed
M2 / 47 / -8183.15 / 2.45 / p0 = 0.255, p1 = 0.190,
(p2 = 0.555), w2 = 0.265 / 315.52
P<0.001 / None
M3 / 49 / -8160.9 / 2.46 / p0 = 0.520, p1 = 0.465,
(p2 = 0.015), w0 = 0.064,
w1 = 0.585, (w2 = 4.453) / 409.10
P<0.001 / >0.99: S18 H47 M204 E296
M7 / 46 / -8180.44 / 2.41 / p = 0.436, q = 0.880 / N/A / Not allowed
M8 / 48 / -8155.89 / 2.48 / p0 = 0.987, (p1 = 0.013),
p = 0.482, q = 1.036,
w = 4.773 / 49.10
P<0.001 / >0.99: S18 H47
>0.95: E296
>0.50: M204
Human Testis OR orthologs: Mouse
M0 / 25 / -5727.3 / 2.66 / w = 0.2906 / N/A / None
M1 / 25 / -5623.64 / 3.03 / p0 = 0.464(p1 = 0.536) / N/A / Not allowed
M2 / 27 / -5615.38 / 3.09 / p0 = 0.464, p1 = 0.533,
(p2 = 0.004), w2 = 12.323 / 16.52
P<0.001 / >0.99: Q47
M3 / 29 / -5563.44 / 2.74 / p0 = 0.590, p1 = 0.335,
(p2 = 0.075), w0 = 0.043,
w1 = 0.621, (w2 = 1.623) / 327.72
P<0.001 / >0.99: Q47
>0.50: K3 P17 P18 M102 G105 L143 H152 T160 Y190 I204 A216 H232 G261 G268 S269
M7 / 26 / -5566.81 / 2.68 / p = 0.228, q = 0.478 / N/A / Not allowed
M8 / 28 / -5560.09 / 2.74 / p0 = 0.997, (p1 = 0.003),
p = 0.230, q = 0.486,
w = 8.606 / 13.44
P<0.01 / >0.99: Q47
V1R vomeronasal receptor
M0 / 43 / -8842.47 / 2.46 / w = 0.6095 / N/A / None
M1 / 43 / -8841.9 / 2.67 / p0 = 0.077(p1 = 0.923) / N/A / Not allowed
M2 / 45 / -8777.4 / 2.45 / p0 = 0.043, p1 = 0.394, (p2 = 0.563),
w2 = 0.372 / 129
P<0.001 / None
M3 / 47 / -8768.11 / 2.51 / p0 = 0.185, p1 = 0.676, (p2 = 0.139), w0 = 0.139, w1 = 0.607, (w2 = 1.766) / 148.7
P<0.001 / >0.99: T71 R77 T269 I277 R301
>0.95: V152 S291 H295
>0.50: I6 T9 S11 R41 P45 A66 G79 L151 P158 M162 N163 L188 Q219 K230 Q238 T255 C259 T262 L265 S270 S272 E274 V284 F299 L300
M7 / 44 / -8782.43 / 2.44 / p = 1.031, q = 0.688 / N/A / Not allowed
M8 / 46 / -8768.57 / 2.52 / p0 = 0.908, (p1 = 0.092), p = 1.451, q = 1.169,
w = 1.989 / 27.72
P<0.001 / >0.99: R77
>0.95: T71 T269 I277 R301
>0.50: I6 S11 R41 G79 L151 V152 P158 M162 N163 L188 K230 Q238 T255 L265 E274 S291 H295 F299
V2R vomeronasal receptor
M0 / 17 / -10414.26 / 2.3 / w = 0.5712 / N/A / None
M1 / 17 / -10268.45 / 2.3 / p0 = 0.412(p1 = 0.588) / N/A / Not allowed
M2 / 19 / -10238.48 / 2.48 / p0 = 0.395, p1 = 0.544, (p2 = 0.061), w2 = 3.783 / 59.94
P<0.001 / >0.99: V73 S406
>0.95: L58 P60 V76 E318 L604
>0.50: F18 A29 K30 L54 K61 L63 D69 D72 M122 R166 S188 T260 L265 K266 H267 G276 L293 I295 F374 K376 R428 L434 D435 S437 R440 G457 N464 Q521 V589 C621 R699 W703
M3 / 21 / -10228.96 / 2.39 / p0 = 0.482, p1 = 0.416, (p2 = 0.101),
w0 = 0.081, w1 = 0.841, (w2 = 2.770) / 370.6
P<0.001 / >0.99: P60 V73 S406
>0.95: L58 V76 T260 L293 E318 L604 C621
>0.50: F18 A29 K30 L54 K61 L63 T68 D69 T70 D72 M122 R166 S188 L265 K266 H267 L274 G276 D286 I295 P299 R321 R324 H325 F374 D375 K376 G378 A412 R428 L434 D435 S437 R440 N453 G457 S459 S460 N464 V465 V466 N520 Q521 A551 V589 L601 S658 R699 W703 V779 S825 R848 D851
M7 / 18 / -10261.38 / 2.22 / p = 0.145, q = 0.140 / N/A / Not allowed
M8 / 20 / -10228.84 / 2.39 / p0 = 0.894, (p1 = 0.106), p = 0.315, q = 0.423,
w = 2.716 / 65.08
P<0.001 / >0.99: V73 S406
>0.95: L58 P60 V76 L293 E318 L604 C621
>0.50: F18 A29 K30 L54 K61 L63 K65 T68 D69 T70 D72 N94 M122 R166 S188 T260 L265 K266 H267 L274 G276 D286 Y292 I295 Y296 P299 R321 R324 H325 F374 D375 K376 G378 A412 R428 L434 D435 S437 R440 N453 G457 S459 S460 N464 V465 V466 N520 Q521 A551 M588 V589 L601 S658 R699 W703 M706 V779 S825 R848 D851
Major Urinary Protein (MUP)
M0 / 29 / -2942 / 1.99 / w = 0.6746 / N/A / None
M1 / 29 / -2918 / 2.08 / p0 = 0.225(p1 = 0.775) / N/A / Not allowed
M2 / 31 / -2907 / 1.93 / p0 = 0.029, p1 = 0.529, (p2 = 0.442), w2 = 0.175 / 20.86
P<0.001 / None
M3 / 33 / -2904 / 2.06 / p0 = 0.101, p1 = 0.522, (p2 = 0.377), w0 = 0.000, w1 = 0.351, (w2 = 1.542) / 76.86
P<0.001 / >0.99: L47 E82 E84 T106 I132 E142
>0.95: A28 V29 T30 L46 D69 T77 H81 T89 N92 L93 P97 I103 D107 L111 D115 N123 K130 M140
>0.50: A20 E22 N25 S31 F33 S36 S39 E41 D50 C51 V63 I83 N87 I91 C95 K96 E99 S105 V113 Y126 F127 Y128 N129 E136 L139 Y144 T147 P148 V159 Y161 E163 D168 L175 V178 G179
M7 / 30 / -2910 / 1.92 / p = 0.393, q = 0.263 / N/A / Not allowed
M8 / 32 / -2904 / 2.06 / p0 = 0.678, (p1 = 0.322), p = 1.180, q = 2.092,
w = 1.621 / 12.16
P<0.01 / 0.99: I132
0.95: L47 E82 E84 E142
0.50: A20 N25 A28 V29 T30 S31 F33 S36 S39 L46 C51 V63 D69 T77 H81 I83 N87 T89 I91 N92 L93 C95 K96 P97 E99 I103 S105 T106 D107 L111 V113 D115 N123 N129 K130 E136 M140 Y144 T147 P148 V159 E163 L175 V178 G179
Odorant Binding Protein (OBP)
M0 / 23 / -5350.95 / 1.73 / w = 0.5596 / N/A / None
M1 / 23 / -5328.22 / 1.92 / p0 = 0.060(p1 = 0.940) / N/A / Not allowed
M2 / 25 / -5306.54 / 2.13 / p0 = 0.059, p1 = 0.716, (p2 = 0.225), w2 = 2.951 / 46.36
P<0.001 / >0.99: Y34 E82 G90
>0.95: Q19 I62 T153
>0.50: H17 Y18 N20 D30 K32 K43 S47 A54 N58 Q66 T67 K69 V76 V79 I85 R88 K94 I97 S101 Q103 Y105 H107 E110 S112 T115 T117 V136 S141 S142 I144 E165
M3 / 27 / -5257.29 / 1.88 / p0 = 0.121, p1 = 0.511, (p2 = 0.367), w0 = 0.056, w1 = 0.445, (w2 = 1.331) / 187.3
P<0.001 / >0.99: Q19 D30 Y34 I62 E82 G90 H107 T153
>0.95: H17 N20 S47 A54 N58 Q66 T67 V76 K94 I97 S101 Y105 E110 S112 S141
>0.50: A8 A10 Y18 V24 S27 K32 K43 I56 K57 K69 T71 T74 E77 G78 V79 I85 R88 Q103 I108 K111 T115 T117 V123 K128 V136 S142 I144 K147 E151 E155 E165 D166 L167 P169 N172 Q175
M7 / 24 / -5263.71 / 1.79 / p = 0.782, q = 0.530 / N/A / Not allowed
M8 / 26 / -5258.36 / 1.89 / p0 = 0.801, (p1 = 0.199), p = 0.984, q = 0.926,
w = 1.625 / 10.7
P<0.01 / >0.95: G90
>0.50: H17 Q19 N20 D30 K32 Y34 K43 S47 A54 N58 I62 Q66 T67 V76 E82 I85 K94 I97 S101 Q103 Y105 H107 E110 S112 T117 S141 I144 T15
Ly-6-homologous Urinary Protein (LUP)
M0 / 79 / -6825.8 / 1.63 / w = 0.6091 / N/A / None
M1 / 79 / -6554.9 / 1.8 / p0 = 0.132(p1 = 0.868) / N/A / Not allowed
M2 / 81 / -6545.3 / 1.95 / p0 = 0.132, p1 = 0.555,
(p2 = 0.313), w2 = 1.772 / 19.32
P<0.001 / >0.99: S17
>0.95: Q12 E16 Q60
>0.50: T2 T5 D7 R8 G18 G20 C21 Q23 P26 S32 L33 T35 Q41 N44 N48 I49 F51 Q56 T57 D59 K63 K69
M3 / 83 / -6519.3 / 1.8 / p0 = 0.145, p1 = 0.409,
(p2 = 0.447), w0 = 0.004, w1 = 0.624, (w2 = 1.223) / 613.08
P<0.001 / >0.99: Q12 E16 S17 G20 T57 Q60
>0.95: T2 T5 D7 R8 G18 C21 S32 Q41 I49 D59 K63 K69
>0.50: I4 E19 Q23 P26 K29 L33 I34 T35 N44 A47 N48 F51 V55 Q56 K68
M7 / 80 / -6523.5 / 1.78 / p = 0.210, q = 0.040 / N/A / Not allowed
M8 / 82 / -6520.9 / 1.89 / p0 = 0.721, (p1 = 0.279),
p = 0.088, q = 0.049,
w = 1.528 / 5.12
P=0.077 / >0.95: S17
>0.50: T2 T5 D7 R8 Q12 E16 G18 G20 C21 Q23 P26 S32 T35 Q41 I49 F51 Q56 T57 D59 Q60 K63 K69
MHC-M10
M0 / 21 / -6973.78 / 2.11 / w = 0.8351 / N/A / None
M1 / 21 / -6893.48 / 2.11 / p0 = 0.183(p1 = 0.817) / N/A / Not allowed
M2 / 23 / -6802.54 / 2.39 / p0 = 0.164, p1 = 0.678, (p2 = 0.158), w2 = 4.550 / 181.88
P<0.001 / >0.99: C10 N11 L45 P68 Q93 R96 T100 N101 R106 I110 Y111 E113 F169 T172 N176 V177 G182 L188
>0.95: F12 E67 T69 R71 D173 A179
>0.50: P4 Q24 T34 L40 Q51 P83 E88 A92 L97 R103 L104 Y107 M109 Q114 F126 G138 I151 S158 A166 L170 E190 T192 C198 R213 Q305
M3 / 25 / -6774.8 / 2.32 / p0 = 0.497, p1 = 0.426, (p2 = 0.077), w0 = 0.252, w1 = 1.433, (w2 = 5.992) / 397.96
P<0.001 / >0.99: P4 C10 N11 F12 T23 Q24 Q33 T34 T37 L40 L45 P47 Q51 G53 E67 P68 T69 A70 R71 Q73 P83 E88 K89 E90 A92 Q93 R96 L97 S98 L99 T100 N101 E102 R103 L104 R106 Y107 M109 I110 Y111 E113 Q114 S115 N116 E117 E118 F126 N132 S135 G138 L140 I151 N152 S158 T161 Q163 A166 F169 L170 T172 D173 L174 N176 V177 G178 A179 G182 L188 E190 T192 R194 C198 T205 R213 L242 K249 Q305
>0.95: G5 L22 W31 T44 K66 M72 E82 V94 L95 D131 F150 A162 G168 K171 T185 D200 L201 S209 H219 Y251 H289 V329
>0.50: N3 P6 L17 V18 A19 L20 C26 A27 F35 N36 V38 I39 K41 E46 V52 S57 Y60 Q61 G62 R75 M79 Q81 P84 E85 Y86 E124 V125 N129 Y141 R142 G147 Y148 S159 E165 V175 A180 E181 W183 R184 E193 L195 R197 E204 R208 T214 P222 E223 P258 S278 P296 I311 L314 G316 G320 L323 V324 W333 K335
M7 / 22 / -6853 / 1.98 / p = 0.422, q = 0.280 / N/A / Not allowed
M8 / 24 / -6783.64 / 2.27 / p0 = 0.830, (p1 = 0.170), p = 0.590, q = 0.432,
w = 3.384 / 138.72
P<0.001 / >0.99: C10 N11 L45 P68 Q93 R96 T100 N101 R106 Y111 T172 N176 V177 G182 L188
>0.95: F12 E67 T69 R71 I110 E113 F169 D173 A179
>0.50: P4 T23 Q24 T34 L40 Q51 Q73 P83 E88 A92 L97 S98 R103 L104 Y107 M109 Q114 F126 G138 I151 S158 A166 L170 E190 T192 C198 R213 Q305