Atentatively Identified Compounds Are Shown in Brackets

Table S1 Mean (±SD) relative composition (%) of cuticular extracts of females and males of Acromis sparsa and Chelymorpha alternans.

RI / Compound(s) / Diagnostic ions / A. sparsa / C. alternans /
/ Females / Males / Females / Males /
2026 / C18al / 250 (M-18), 224 (M-44) / 3.4±1.1 / 2.4±1.4 / 0.2±0.3 / 0.3±0.2
2109 / 2-C19one / 58, 282 / 0.2±0.1 / 0.2±0.0 / tr / -
2230 / C20al / 278 (M-18), 252(M-44) / 0.1±0.1 / tr / 0.1±0.1 / 0.2±0.1
2400 / n-C24 / 338 / 0.5±0.2 / 0.2±0.2 / - / -
2434 / 11-/12-MeC24 / 168/210, 182/196, 337 (M-15) / 0.6±0.2 / 0.6±0.2 / tr / tr
2474 / C25ene / 350 / 0.2±0.3 / 0.1±0.1 / tr / -
2500 / n-C25 / 352 / 3.4±1.3 / 3.8±2.2 / 0.2±0.2 / 0.2±0.1
2535 / 13-MeC25 / 196 (2x), 351 (M-15) / 1.6±0.3 / 1.8±0.7 / tr / tr
2579 / non-CHC (δ-eicosalactone)a / 99, 114, 248, 292, 310 / - / - / 0.5±0.5 / 0.9±0.5
2580 / C26ene / 364 / 0.2±0.1 / tr / - / -
2600 / n-C26 / 366 / 0.4±0.1 / 0.3±0.2 / tr / 0.2±0.1
2633 / 12-/13-MeC26 / 182/224, 196/210, 365 (M-15) / 1.0±0.2 / 1.0±0.4 / tr / tr
2661 / 2-MeC26 / 337 (M-43), 365 (M-15) / 0.1±0.1 / 0.1±0.1 / 0.2±0.3 / 0.6±0.4
2686 / non-CHC (δ-heneicosalactone) / 99, 114, 262, 306, 324 / tr / tr / tr / 0.5±1.4
2700 / n-C27 / 380 / 4.0±1.6 / 3.3±3.1 / 1.5±1.5 / 5.5±3.8
2732 / 13-MeC27 / 196/224 / 0.1±0.1 / 0.1±0.0 / 0.2±0.3 / 0.2±0.1
2762 / 2-MeC27 / 351 (M-43), 379 (M-15) / - / - / 0.1±0.2 / 0.2±0.1
2781 / 5,15-diMeC27 / 85/351, 196/239, 393 (M-15) / - / - / 0.2±0.6 / 0.6±0.9
2792 / non-CHC (δ-docosalactone) / 99, 114, 276, 320, 338 / 0.7±0.4 / 0.7±0.5 / 1.1±0.7 / 1.3±0.8
2800 / n-C28 / 394 / 1.0±0.4 / 0.8±0.6 / 0.7±0.7 / 1.6±0.4
2862 / 2-MeC28 / 365 (M-43), 393 (M-15) / 0.1±0.0 / tr / 1.1±1.0 / 5.3±2.4
2878 / C29diene / 404 / tr / tr / 0.2±0.2 / 1.7±0.9
2900 / n-C29 / 408 / 1.3±0.5 / 1.6±0.9 / 1.9±0.8 / 2.8±0.8
2930 / 11-/13-MeC29 / 168/280, 196/252, 407 (M-15) / tr / tr / tr / 0.2±0.2
2942 / 7-MeC29 / 112/337, 407 (M-15) / tr / tr / 0.1±0.2 / 0.4±0.4
2944 / non-CHC (C27ol:Ac) / 43, 61, 378 / - / - / 0.3±0.9 / 1.3±1.3
2973 / 7,17-diMeC29 / 112/351, 196/267, 421(M-15) / tr / tr / 0.2±0.4 / 0.9±0.7
3000 / n-C30 / 422 / 0.5±0.2 / 0.8±0.4 / 1.3±1.7 / tr
3006 / non-CHC (δ-tetracosalactone) / 99, 114, 304, 348, 366 / - / tr / 0.2±0.2 / 0.3±0.2
3018 / non-CHC (C27ol:Pr) / 57, 75, 378 / - / tr / 0.2±0.5 / 0.3±0.4
3031 / 12-MeC30 / 182/280, 421 (M-15) / 0.8±0.2 / 1.0±0.2 / tr / -
3042 / non-CHC (C28ol:Ac) / 43, 61, 392 / - / tr / 0.1±0.3 / 0.5±0.4
3061 / 2-MeC30 / 393 (M-43), 421 (M-15) / 0.1±0.1 / tr / 2.7±1.9 / 0.2±0.1
3075 / ?/C31diene/C31ene / 432; 434 / tr / tr / 1.0±0.5 / 0.9±0.7
3083 / non-CHC/C31ene / 211/295, 225/281; 434 / 0.1±0.1 / 0.3±0.4 / 0.2±0.3 / 0.2±0.3
3100 / n-C31 / 436 / 2.5±0.6 / 3.2±1.3 / 5.5±7.5 / 0.4±0.3
3106 / non-CHC (C29ol:Ac) / 43, 61, 406 / - / tr / 0.5±1.1 / 0.6±0.6
3116 / non-CHC (C28ol:Pr) / 57, 75, 392 / - / - / tr / 0.3±0.3
3128 / 11-/13-MeC31 / 168/308, 196/280, 435 (M-15) / 1.3±0.4 / 1.2±0.2 / tr / -
3139 / non-CHC (C29ol:Ac) / 43, 61, 406 / - / - / 0.4±0.5 / 1.4±0.9
3159 / 11,19-diMeC31 / 168/323, 196/295, 449 (M-15) / tr / tr / 0.1±0.2 / 0.2±0.2
3179 / non-CHC / 225/295 / - / tr / 0.8±1.8 / 1.5±1.1
3200 / n-C32 / 450 / 0.2±0.1 / 0.2±0.1 / 0.9±1.9 / tr
3213 / non-CHC (C29ol:Pr) / 57, 75, 406 / - / - / 0.3±0.4 / 0.7±0.4
3227 / 12-/14-MeC32 / 182/308, 210/280, 449 (M-15) / 2.4±0.6 / 2.2±0.4 / tr / -
3261 / 11,21-diMeC32 / 168/337, 182/323, 463 (M-15) / 0.7±0.2 / 0.7±0.2 / 0.3±0.5 / tr
3260 / 2-MeC32 / 421 (M-43), 449 (M-15) / - / - / 0.2±0.2 / -
3275 / C33ene / 462 / - / - / 0.1±0.2 / -
3279 / non-CHC / 225/309, 253/281 / 0.6±0.3 / 0.9±0.5 / 0.6±0.5 / 0.6±0.3
3281 / C33ene / 462 / - / - / 0.3±0.4 / -
3300 / n-C33 / 464 / 0.3±0.2 / 0.4±0.2 / 1.6±2.6 / tr
3307 / non-CHC (C30ol:Pr) / 57, 75, 420 / tr / tr / 0.2±0.2 / tr
3327 / 11-/13-MeC33 / 168/336, 196/308, 463 (M-15) / 1.4±0.4 / 1.2±0.2 / 0.4±0.3 / 0.2±0.1
3338 / non-CHC (C31ol:Ac) / 43, 61, 434 / tr / tr / 0.9±0.8 / 0.4±0.4
3353 / 13,17-diMeC33 / 196/323, 252/267, 477 (M-15) / - / - / 0.1±0.2 / -
3358 / 11,21-diMeC33 / 168/351, 196/323, 477 (M-15) / 0.6±0.2 / 0.6±0.3 / 0.5±0.8 / 0.4±0.3
3365 / non-CHC(C31ol:Pr) / 57, 75, 434 / - / tr / - / 0.2±0.3
3376 / 13,17,21-triMeC33 / 196/337 (2x), 267 (4x), 491 (M-15) / - / - / 0.1±0.3 / -
3380 / non-CHC / 225/323 / tr / tr / 0.8±0.9 / 2.5±1.5
3409 / non-CHC (C31ol:Pr) / 57, 75, 434 / - / - / 0.3±0.1 / 0.2±0.2
3427 / 13-/14-MeC34 / 196/322, 210/308, 477 (M-15) / 0.8±0.2 / 0.7±0.1 / 0.4±0.3 / 0.1±0.1
3456 / 12,22-diMeC34 / 182/351, 336/337, 491 (M-15) / 0.3±0.2 / 0.4±0.1 / 1.0±0.8 / 0.9±0.5
3481 / non-CHC / 225/337 / tr / tr / tr / 0.6±0.6
3500 / n-C35 / 492 / - / tr / 0.2±0.6 / -
3527 / 13-MeC35 / 196/336, 492 (M-15) / 0.3±0.1 / 0.3±0.1 / 2.8±1.4 / 1.4±0.4
3552 / 15,19-diMeC35 / 224/323, 252/295, 506 (M-15) / - / - / 0.7±1.8 / -
3556 / 13,23-diMeC35 / 196/351 (2x), 506 (M-15) / tr / tr / 5.7±2.8 / 6.3±1.8
3562 / 11,x-diMeC35 / 168/379, 506 (M-15) / - / - / 1.3±1.5 / 2.2±1.2
3575 / 9,13-diMeC35 / 140/407, 211/308, 506 (M-15) / - / - / 0.5±1.2 / -
3579 / 11,15,x-triMeC35 / 168/393, 239/323, 520 (M-15) / - / - / 0.7±0.8 / 1.1±0.6
3580 / non-CHC / 225/351, 253/323 / 0.1±0.2 / 0.1±0.1 / - / tr
3588 / non-CHC / 197/379 / ± / tr / 0.1±0.1 / 0.6±0.4
3627 / 12-/13-/14-MeC36 / 182/364, 196/350, 210/336, 506 (M-15) / 1.4±0.2 / 1.4±0.2 / 1.0±0.5 / 0.6±0.3
3649 / 14,x-diMeC36 / 210/351, 520 (M-15) / - / - / 0.2±0.5 / -
3655 / 12,22-diMeC36 / 182/379, 224/337, 520 (M-15) / 0.8±0.2 / 1.1±0.2 / 2.5±1.1 / 3.4±1.1
3669 / C37ene / 518 / - / - / 0.2±0.4 / -
3678 / 12,x,x-triMeC36 / 182/393, 534 (M-15) / - / - / 0.2±0.3 / 0.2±0.1
3690 / 2,12-/2,22-/2,24-diMeC36 / 196/365, 197/364, 224/337, 520 (M-15) / 1.7±0.4 / 1.1±0.5 / tr / tr
3718 / 2,12,22-triMeC36 / 196/379, 225/352, 534 (M-15) / 0.6±0.3 / 0.5±0.3 / - / -
3728 / 13-/15-diMeC37 / 196/364, 224/336, 520 (M-15) / 1.8±0.4 / 1.5±0.4 / 4.6±1.1 / 3.3±0.7
3752 / 15,19-diMeC37 / 224/351, 280/295, 534 (M-15) / - / - / 1.6±3.7 / -
3757 / 13,23-diMeC37 / 196/379, 224/351, 534 (M-15) / 2.6±0.5 / 3.0±0.6 / 7.5±2.9 / 9.6±3.5
3759 / 11,x-diMeC37 / 168/408, 534 (M-15) / - / - / 4.9±4.4 / 8.3±2.6
3776 / 13,17,21-triMeC37 / 196/393, 252/337, 267/323, 548 (M-15) / - / - / 0.6±1.3 / -
3778 / 11,15,x-triMeC37 / 168/421, 239/351, 548 (M-15) / - / - / 1.2±0.9 / 2.7±3.0
3781 / non-CHC / 281/323 / 0.6±0.3 / 0.3±0.4 / - / -
3789 / non-CHC / 197/407 / 0.7±0.2 / 1.1±0.5 / 0.4±0.4 / 0.6±0.4
3829 / 12-/14-MeC38 / 182/392, 210/364, 534 (M-15) / 2.0±0.4 / 1.7±0.3 / 1.1±0.5 / 0.9±0.4
3850 / 14,x-diMeC38 / 210/379, 548 (M-15) / - / - / 0.2±0.5 / -
3858 / 12,22-diMeC38 / 182/407, 252/337, 548 (M-15) / 14.1±1.9 / 16.1±1.8 / 4.4±2.4 / 4.4±1.8
3870 / C39ene / 546 / - / - / 0.1±0.4 / -
3879 / 12,x,x-triMeC38 / 182/421, 562 (M-15) / - / - / 0.3±0.5 / 0.5±0.5
3885 / non-CHC / 281/337 / 1.0±0.5 / 0.5±0.5 / tr / -
3889 / 2,12/2,14-/2,22-diMeC38 / 197/392, 225/364, 252/337, 548 (M-15) / 1.6±0.2 / 2.1±0.4 / tr / 0.2±0.1
3910 / non-CHC / 281/351 / 0.9±0.4 / 0.8±0.3 / - / -
3918 / 2,12,22-triMeC38 / 197/407, 252/351, 562 (M-15) / 5.8±1.7 / 3.8±2.3 / tr / -
3928 / 13-MeC39 / 196/392, 548 (M-15) / 0.8±0.6 / 0.8±0.3 / 2.1±0.9 / 1.6±0.7
3956 / 15,x-/13,23-diMeC39 / 196/407, 224/379/252/351, 562 (M-15) / 7.9±1.3 / 8.5±1.6 / 11.7±3.9 / 8.8±1.9
3973 / 13,x,x-triMeC39 / 196/421, 576 (M-15) / - / - / 0.8±1.9 / -
3974 / 11,15,x-triMeC39 / 168/449, 239/379, 576 (M-15) / - / - / 1.2±1.2 / 0.9±0.5
3985 / non-CHC / 253/379 / 2.8±0.8 / 2.9±0.8 / 0.3±0.4 / 0.3±0.2
-b / non-CHC / 281/365 / 0.7±0.2 / 0.5±0.2 / - / -
- / 12-/14-MeC40 / 182/420, 210/392, 562 (M-15) / 0.4±0.2 / 0.3±0.2 / 0.3±0.2 / 0.3±0.1
- / 14,x-diMeC40 / 210/407, 576 (M-15) / - / - / 0.2±0.5 / -
- / 12,22-diMeC40 / 182/435, 280/337, 576 (M-15) / 6.8±0.8 / 6.9±0.7 / 2.1±1.7 / 0.9±0.4
- / non-CHC / 323/449 / 0.6±0.4 / 0.4±0.3 / - / -
- / non-CHC / 253/393, 267/379 / 1.1±0.5 / 1.4±0.6 / tr / -
- / non-CHC / 281/379, 309/351 / 0.3±0.2 / 0.2±0.2 / - / -
- / 2,12,22-triMeC40 / 197/435, 280/351, 590 (M-15) / 1.4±0.3 / 0.9±0.4 / tr / -
- / 13-/15-/17-MeC41 / 196/392, 576 (M-15) / - / - / 0.4±0.2 / 0.4±0.1
- / 15,25-diMeC41 / 225/407, 225/379, 590 (M-15) / - / - / 0.6±1.3 / -
- / 13,23-diMeC41 / 196/435, 280/351, 590 (M-15) / 1.6±0.3 / 1.9±0.5 / 3.1±2.6 / 0.8±0.2
- / 13,x,x-triMeC41 / 196/449, 604 (M-15) / - / - / 0.2±0.4 / -
- / non-CHC / 281/379 / 0.7±0.3 / 1.3±0.7 / tr / -
- / non-CHC / 281/393 / 0.3±0.1 / 0.2±0.1 / - / -
- / 12,22-diMeC42 / 182/463, 308/337, 604 (M-15) / 1.8±0.5 / 1.7±0.3 / 0.3±0.4 / tr
- / non-CHC / 281/393, 295/379 / 0.3±0.2 / 0.6±0.2 / - / -
- / non-CHC / 281/407 / 0.6±0.2 / 0.7±0.3 / - / -
- / 13,23-diMeC43 / 196/463, 308/351, 618(M-15) / 0.5±0.2 / 0.5±0.2 / 0.1±0.3 / -
- / non-CHC / 281/421, 309/393 / 0.2±0.1 / 0.2±0.1 / - / -
- / 12,22-diMeC44 / 182/492, 336/337, 632 (M-15) / 1.6±0.7 / 1.5±0.5 / tr / -
- / non-CHC / 309/407 / 0.2±0.1 / 0.2±0.1 / - / -
- / 13,23-diMeC45 / 196/491, 336/351, 646 (M-15) / 0.3±0.1 / 0.2±0.1 / tr / -

atentatively identified compounds are shown in brackets

bno reference alkanes were available for the computation of the retention indices