APPENDIX 1 - SUPPLEMENTARY MATERIAL
Contrasting post-settlement selection results in many-to-one mapping of high performance phenotypes in the Hawaiian waterfall-climbing goby Sicyopterus stimpsoni.
K. N. Moody^, 1, 2, S. M. Kawano^, 2, 3, W. C. Bridges4, R. W. Blob2, H. L. Schoenfuss5, and M. B. Ptacek2
^ Co-first authorship
1 The ByWater Institute, Tulane University, 627 Lindy Boggs Center, 6823 St. Charles Ave., New Orleans, LA, 70118, USA.
2 Department of Biological Sciences, 132 Long Hall, Clemson University, Clemson, SC 29634, USA.
3 National Institute for Mathematical and Biological Synthesis, 1122 Volunteer Blvd., Suite 106, University of Tennessee, Knoxville, TN 37996, USA.
4 Department of Mathematical Sciences, O-110 Martin Hall, Clemson University, Clemson, SC 29634, USA.
5 Aquatic Toxicology Laboratory, 720 Fourth Ave S, St. Cloud State University, WSB-273, St. Cloud, MN 56301, USA
Evaluating the propensity to climb the artificial waterfall
Additional data collected during the laboratory climbing selection trials indicated that there was a relatively high propensity to climb the artificial waterfall (Table S1). On average (mean ± s.d.), the percent of the individuals within a trial that attempted to climb the artificial waterfall was 97.69 ± 3.17 for the Big Island and 88.35 ± 6.85 for Kaua’i. These values ranged from 75 - 100%. Also, individuals began climbing within a relatively short time after the experimental trial began (denoted by the commencement of the stream of water flowing down the chute), with one individual that started to climb after only 9 seconds had elapsed (trial 17 for Waimea). Together, these data suggest that the motivation to climb the artificial waterfall was high.
Table S1. Proportion of individuals that attempted to climb during each experimental trial
Island / Stream / Trial / Total Inds / # Inds Climbing / % Inds Climbing / Time to First Climb (mins)Kaua’i / Waimea / 8 / 20 / 16 / 80.00 / 6:18
Kaua’i / Waimea / 9 / 20 / 17 / 85.00 / 2:02
Kaua’i / Waimea / 10 / 20 / 17 / 85.00 / 5:21
Kaua’i / Waimea / 11 / 20 / 15 / 75.00 / 2:59
Kaua’i / Waimea / 12 / 21 / 18 / 85.71 / 3:02
Kaua’i / Waimea / 15 / 21 / 20 / 95.24 / 1:45
Kaua’i / Waimea / 20 / 11 / 10 / 90.91 / 1:01
Kaua’i / Waimea / 22 / 18 / 18 / 100.00 / 5:40
Kaua’i / Waimea / 27 / 20 / 19 / 95.00 / 1:49
Kaua’i / Waimea / 17 / 20 / 18 / 90.00 / 0:09
Kaua’i / Waimea / 26 / 20 / 18 / 90.00 / 1:38
Big Island / Hakalau / 35 / 19 / 19 / 100.00 / 2:00
Big Island / Hakalau / 37 / 19 / 19 / 100.00 / 4:44
Big Island / Hakalau / 36 / 20 / 20 / 100.00 / 3:30
Big Island / Hakalau / 38 / 20 / 19 / 95.00 / 3:00
Big Island / Hakalau / 39 / 20 / 19 / 95.00 / 2:39
Big Island / Hakalau / 40 / 20 / 19 / 95.00 / 3:07
Big Island / Hakalau / 41 / 20 / 20 / 100.00 / 2:43
Big Island / Hakalau / 42 / 20 / 18 / 90.00 / 3:20
Big Island / Hakalau / 43 / 20 / 20 / 100.00 / 2:03
Big Island / Hakalau / 44 / 19 / 19 / 100.00 / 5:38
Big Island / Hakalau / 45 / 20 / 20 / 100.00 / 2:44
Big Island / Hakalau / 46 / 20 / 19 / 95.00 / 4:36
Big Island / Hakalau / 47 / 20 / 20 / 100.00 / 4:47
Values were based on data taken before outliers were removed and, therefore, may indicate a slightly greater number of individuals per trial than was included in the statistical analysis of phenotypic selection.
“Total inds”: total number of individuals within that experimental trial
“# Inds Climbing”: maximum number of individuals that climbed during the experimental trial
“% Climbing”: percentage of the maximum number of individuals that climbed during the trial
“Time to First Climb”: time elapsed before first individual attempted to climb (in minutes:seconds)