Supplementary Information for

A peptide release system using a photo-cleavable linker in a cell array format for cell-toxicity analysis

Takashi Kakiyama§1, Kenji Usui§1,2,3, Kin-ya Tomizaki4, Masayasu Mie2, Eiry Kobatake2 and Hisakazu Mihara1

§ These authors contributed equally to this work.

1Department of Bioengineering and 2Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226-8501, Japan, 3FIRST (Faculty of Frontiers of Innovative Research in Science and Technology) and FIBER (Frontier Institute for Biomolecular Engineering Research), Konan University, Kobe 650-0047, Japan, 4Innovative Materials and Processing Research Center and Department of Materials Chemistry, Ryukoku University, Otsu 520-2194, Japan

Correspondence: Professor H. Mihara, Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho 4259-B40, Midori-ku, Yokohama 226-8501, Japan

E-mail: , Tel: +81-45-924-5756, FAX: +81-45-924-5833

Assistant Professor K. Usui, FIRST (Faculty of Frontiers of Innovative Research in Science and Technology) and FIBER (Frontier Institute for Biomolecular Engineering Research), Konan University, 7-1-20 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047, Japan

E-mail: , Tel:+81-78-303-1418, FAX:+81-78-303-1495

Figure S1. Relative cell viability of the free TMR-KLA-C peptide in solution against (a) HeLa and (b) 10T1/2 cells as determined using the Cell Counting Kit-8. The relative viability was calculated by dividing the absorbance of tested cells by that of control cells incubated without the peptides (assuming that 100% viability = 0% toxicity).

Figure S2. Cell-viability assay after overnight culture with/without the TMR-KLA-C immobilization against (a) HeLa, (b) 10T1/2 and (c) COS7 cells as determined using the Cell Counting Kit-8. The relative viability was calculated by dividing the absorbance of tested cells by that of control cells on non-coated plate (without the immobilized peptide and PLK) (assuming that 100% viability = 0% toxicity).

Figure S3. Cell-toxicity assay with the photo-release system using HeLa and COS7 cells with TMR-L8K6-C (TMR-G-LKKLLKLLKKLLKL-G-C-NH2). The relative viability was calculated by dividing the absorbance of tested cells by that of control cells on non-coated well with light-irradiation for 60 min (assuming that 100% viability = 0% toxicity).

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