Table S1. Baseline demographics of patients with renal cell carcinoma who underwent radical nephrectomy

Characteristics / Total (N, %)
No. of patients / 324
Age at surgery
Median (years) / 55 (48 – 64)
< 60 / 201 (62.0)
≥ 60 / 123 (38.0)
Sex
Female / 83 (25.6)
Male / 241 (74.4)
BMI (kg/m2)
Median / 24.27 (22.17 – 26.23)
25 / 194 (59.9)
≥25 / 130 (40.1)
Diabetes mellitus
No / 282 (87.0)
Yes / 42 (13.0)
Preoperative PNI
Median / 45.00 (42.01 – 46.51)
≤ 45 / 161 (49.7)
> 45 / 163 (50.3)
Postoperative PNI (at 6 months)
Median / 45.01 (43.01 – 47.01)
≤ 45 / 141 (43.5)
> 45 / 183 (56.5)
†PNI dynamics
Group 1 (Low → Low) / 92 (28.4)
Group 2 (Low → High) / 69 (21.3)
Group 3 (High → Low) / 48 (14.8)
Group 4 (High → High) / 115 (35.5)
Laterality
Left / 155 (47.8)
Right / 169 (52.2)
Tumor size (cm)
Median / 5.0 (3.5 – 7.0)
7 / 249 (76.9)
≥7 / 75 (23.1)
Histology
Clear cell / 278 (86.6)
Non-clear cell / 43 (13.4)
pT stage
pT1 / 204 (63.0)
pT2 / 28 (8.7)
pT3 / 88 (27.1)
pT4 / 4 (1.2)
Fuhrman grade
G1 / 5 (1.6)
G2 / 79 (24.6)
G3 / 199 (62.0)
G4 / 38 (11.8)
Oncological outcomes
Recurrence / 29 (9.0)
All-cause death / 44 (13.6)
Cancer-specific death / 34 (10.5)

Table S2. Univariate and multivariate analyses of predictors of cancer-specific survival in patients with localized renal cell carcinoma (pT1 and 2) who underwent radical nephrectomy

Variables / Univariate / Multivariate
HR / 95% CI / P / HR / 95% CI / P
Age at surgery
< 60 years / Reference
≥ 60 years / 1.19 / 0.49 – 2.84 / 0.693
Sex
Female / Reference
Male / 0.86 / 0.33 – 2.19 / 0.750
BMI (kg/m2)
25 / Reference
≥25 / 0.39 / 0.14 – 1.07 / 0.067
Preoperative PNI
≤ 45 / Reference / Reference
> 45 / 0.18 / 0.06 – 0.51 / 0.002 / 0.25 / 0.08 – 0.76 / 0.014
Postoperative PNI (at 6 months)
≤ 45 / Reference / Reference
> 45 / 0.26 / 0.10 – 0.68 / 0.006 / 0.28 / 0.10 – 0.73 / 0.010
PNI dynamics
Low → Low / Reference / Reference
Low → High / 0.43 / 0.15 – 1.21 / 0.113 / 0.34 / 0.12 – 0.97 / 0.045
High → Low / 0.30 / 0.08 – 1.07 / 0.064 / 0.35 / 0.10 – 1.25 / 0.107
High → High / 0.05 / 0.01 – 0.35 / 0.003 / 0.04 / 0.01 – 0.33 / 0.003
Tumor size (cm)
7 / Reference
≥7 / 4.65 / 1.99 – 10.88 / <0.001 / 4.99 / 2.11 – 11.78 / <0.001
Histology
Clear cell / Reference
Non-clear cell / 1.55 / 0.53 – 4.52 / 0.422
Fuhrman grade
Low (G1 – 2) / Reference
High (G3 – 4) / 1.11 / 0.47 – 2.61 / 0.805

Table S3. Univariate and multivariate analyses of predictors of overall survival in patients with localized renal cell carcinoma (pT1 and 2) who underwent radical nephrectomy

Variables / Univariate / Multivariate
HR / 95% CI / P / HR / 95% CI / P
Age at surgery
< 60 years / Reference
≥ 60 years / 1.58 / 0.75 – 3.36 / 0.227
Sex
Female / Reference
Male / 0.68 / 0.31 – 1.45 / 0.337
BMI (kg/m2)
25 / Reference / Reference
≥25 / 0.36 / 0.14 – 0.89 / 0.028 / 0.66 / 0.25 – 1.75 / 0.407
Preoperative PNI
≤ 45 / Reference / Reference
> 45 / 0.16 / 0.06 – 0.44 / <0.001 / 0.24 / 0.09 – 0.63 / 0.004
Postoperative PNI (at 6 months)
≤ 45 / Reference / Reference
> 45 / 0.23 / 0.10 – 0.54 / 0.001 / 0.26 / 0.10 – 0.62 / 0.003
PNI dynamics
Low → Low / Reference / Reference
Low → High / 0.31 / 0.11 – 0.83 / 0.020 / 0.26 / 0.09 – 0.70 / 0.008
High → Low / 0.21 / 0.06 – 0.73 / 0.014 / 0.24 / 0.07 – 0.82 / 0.022
High → High / 0.06 / 0.02 – 0.28 / <0.001 / 0.06 / 0.01 – 0.26 / <0.001
Tumor size (cm)
7 / Reference / Reference
≥7 / 3.23 / 1.54 – 6.78 / 0.002 / 3.50 / 1.65 – 7.40 / 0.001
Histology
Clear cell / Reference
Non-clear cell / 1.92 / 0.78 – 4.71 / 0.152
Fuhrman grade
Low (G1 – 2) / Reference
High (G3 – 4) / 1.09 / 0.52 – 2.32 / 0.811