Nanomaterials (NMs) Use Form
Principal Investigator: Ph.: Date:
Primary Lab Contact for NMs work: Ph.: e-mail:
Dept.:
Location for proposed work (building and rooms):
1 / Type of NMs:2 / Source:
a.Synthesized in my lab
b. Modified in my lab- please provide name of the manufacturer:
c. Used as purchased - please provide name of the manufacturer:
3 / Is the nanomaterial use dry? yes no
4 / NMs are weighed and handled in:
a. Chemical fume hood
b. Biosafety cabinet
c. Enclosure hood
*Note: Dry NMs should not be weighed or handled on the lab bench without the engineering controls such as local fume hoods*
5 / Frequency of dry NMs handling (ex. 2X per month):per day per week per month per year
6 / What is the typical amount of NMs used per weighing or preparation (ex. 10mg, 20mg, 100mg, etc.)?
7 / Used for animal research? yes no
8 / Used for animal toxicology work? yes no
9 / Used for in vivo study? yes no
10 / Used for in vitro study? yes no
11 / What is the route of application?
a. Topical
b. Injection
c.Aerosols (inhalation)
d. Single dose
e. Multiple doses
12 / Concentration: mg/ml
13 / Total Volume: ml
14 / Duration of aerosol generation: mins
15 / Frequency of use (ex. 1X per week): per day per week per month
Reconstitution of NMs and Usage
NM Name / Maximum Weighed NM Quanity per use (mg) / Solvent/Buffer Used for NM Reconstitution / Maximum Use NM Conc. (in solution/dispersion) / Solution contains multiple NMs (Yes/No) / NM Solution contains other biological/chemical species (yes/No)Example- TiO2 + ZnO / 2mg, each / Hexane/oleic acid / 1mg/ml / Yes, 2 components / Yes, endotoxin + acyclovir
OLS recommends the use of the following PPE and practices while working with NMs.
PPE: At minimum, nitrile gloves, safety glasses and lab coat must be used. Respiratory protection with N95 dust masks or better may be required while working with dry powders and aerosols. Lab coats of impervious material (such as Tyvek) and double nitrile gloves are recommended to protect against the animal skin/hair shedding and NMs.
Engineering Control: Perform all NMs work within a chemical fume hood, BSC ducted to the outside or enclosure hood. If needed, animal work on the work bench/operation table should be carried out under a BSC or fume hood exhausted outside.
Quantity: Use lowest concentration and lowest quantity of NMs possible (typically 1mg/ml in solution).
Cleaning: Clean the work benches, animal cages and bins/carriers by wet wipe method, using paper towels soaked with freshly prepared 10% bleach followed by alcohol-wipe, and then rinse-off/wet wipe the cages and work benches with water. If required, the dry cleaning of work bench or animal shedding should be performed with a vacuum equipment fitted with HEPA filter and ducted outside (or fume hood).
Waste Management: NM-containing waste should be treated as hazardous waste. Laboratory generated NMs waste must not be disposed of in the regular trash or down the drain. NM-containing wastes including NM dispersions must be disposed of through the Office of Health and Safety (4-4347). NMs waste may be stored in a fume hood until ready for pickup, but must be stored in a closed container. Disposable gowns, gloves, sharps and needles should be disposed of as bio-hazardous waste.
Nanomaterial usage formIBC#:10/25/17