Electronic supplemental material of article submitted to World Journal of Microbiology and Biotechnology:

Communities of endophytic microorganisms in different developmental stages from a local variety, transgenic and conventional isogenic hybrids of maize

Kelly Justin da Silva1, Rafael Dutra de Armas2, Cláudio Roberto F.S. Soares2, Juliana Bernardi Ogliari*1

1 Department of Plant Science, University of Santa Catarina, 88034-001, Florianópolis, SC, Brazil.

2 Department of Microbiology, Imunology and Parasitology, University of Santa Catarina, 88040-900, Florianópolis, SC, Brazil.

* Corresponding author: Juliana B. Ogliari, Departamento de Fitotecnia, Universidade Federal de Santa Catarina, CP 476, 88034-001, Florianópolis, SC, Brazil; Phone: 55 48 3721 5327; e-mail:

Figure S1. Image of agarose gel (2%) with the partial amplification of the TC1507 (pat transgene region) with approximately 279 pb. RS (Rosado), NGM (conventional hybrid), Hx (transgenic hybrid with gene TC1507) and Hxrr (transgenic hybrid with genes TC1507+Hxrr) refer to the maize genotypes. C refers to water sample, the negative control.

Figure S2. Bacterial 16S rDNA gene amplicon profiles from the different maize genotypes of four phenological stages after DGGE. The four stages are represented by V1, V2, R1 and R2, when the maize leaves were collected at 28, 50, 73, and 91 days after seeding, respectively. The samples were RS (Rosado), NGM (conventional hybrid), Hx (transgenic hybrid with TC1507 event) and Hxrr (transgenic hybrid with TC1507 and NK603 events). M, molecular marker. Numbers represent individual samples. The samples were collected in the Experiment I.

Figure S3. Fungal ssu (18S rDNA) gene amplicon profiles from the different maize genotypes of four phenological stages after DGGE. The four stages are represented by V1, V2, R1 and R2, when the leaves were collected at 28, 50, 73, and 91 days after seeding, respectively. The samples were RS (Rosado), NGM (conventional hybrid), Hx (transgenic hybrid with TC1507 event) and Hxrr (transgenic hybrid with TC1507 and NK603 events). M, molecular marker. Numbers represent individual samples. The samples were collected in the Experiment I.

Table S1. ANOSIM pairwise comparisons of the endophytic community structures based on the profiles of bacterial 16S rDNA gene amplicons after DGGE, in the Experiment I, four stages

Treatment † / RS* / NGM / Hx / Hxrr
V1 Stage
RS / -
NGM / 0.148 / -
Hx / 0.333 / 0.000 / -
Hxrr / 0.333 / 0.000 / 0.000 / -
V2 Stage
RS / -
NGM / 0.000 / -
Hx / 0.333 / 0.333 / -
Hxrr / 0.167 / 0.167 / 0.000 / -
R1 Stage
RS / -
NGM / 0.000 / -
Hx / 0.000 / 0.000 / -
Hxrr / 0.000 / 0.000 / 0.000 / -
R2 Stage
RS / -
NGM / 0.000 / -
Hx / 0.000 / 0.000 / -
Hxrr / 0.000 / 0.000 / 0.000 / -

† Values represent the pairwise R. ANOSIM R values were derived from Jaccard similarity matrices calculated using binary data representing the presence or absence of 16S rRNA gene amplicons in DGGE profiles (R global = 0.815; P < 0.001).

* The four stages are represented by V1, V2, R1 and R2, when the leaves were collected at 28, 50, 73, and 91 days after seeding, respectively. The samples were RS (Rosado), NGM (conventional hybrid), Hx (transgenic hybrid with TC1507 event) and Hxrr (transgenic hybrid with TC1507 and NK603 events).

Table S2. ANOSIM pairwise comparisons of the endophytic community structures based on the profiles of fungal ssu 18S rDNA gene amplicons after DGGE, in the Experiment I, four stages

Treatment † / RS* / NGM / Hx / Hxrr
V1 Stage
RS / -
NGM / 1.000
Hx / 1.000 / 1.000
Hxrr / 1.000 / 1.000 / 0.000 / -
V2 Stage
RS / -
NGM / 1.000
Hx / 1.000 / 1.000
Hxrr / 1.000 / 1.000 / 0.000 / -
R1 Stage
RS / -
NGM / 1.000
Hx / 1.000 / 0.000
Hxrr / 1.000 / 1.000 / 1.000 / -
R2 Stage
RS / -
NGM / 1.000
Hx / 1.000 / 0.000
Hxrr / 1.000 / 0.000 / 0.000 / -

†Values represent the pairwise R. ANOSIM R values were derived from Jaccard similarity matrices calculated using binary data representing the presence or absence of ssu 18S rDNA gene amplicons in DGGE profiles (R global = 0.917; P < 0.001).

* The four stages are represented by V1, V2, R1 and R2, when the leaves were collected at 28, 50, 73, and 91 days after seeding, respectively. The samples were RS (Rosado), NGM (conventional hybrid), Hx (transgenic hybrid with TC1507 event) and Hxrr (transgenic hybrid with TC1507 and NK603 events).

Table S3. ANOSIM pairwise comparisons of the endophytic community structures based on the profiles of fungal ssu 18S rDNA gene amplicons after DGGE, in the Experiment II, R1 stage

Treatment† / RS / NGM / Hx / Hxrr
R1 Stage *
RS / -
NGM / 1.000 / -
Hx / 1.000 / 0.000 / -
Hxrr / 1.000 / 1.000 / 1.000 / -

† (R global = 0.833, P < 0.001).

* R1 Stage: when the leaves were collected at 73 days after seeding. The samples were RS (Rosado), NGM (conventional hybrid), Hx (transgenic hybrid with TC1507 event) and Hxrr (transgenic hybrid with TC1507 and NK603 events).