Supporting Information

On the Photophysical Properties of New Luminol Derivatives and their Synthetic Phthalimide Precursors

Raúl Pérez-Ruiz,a Robert Fichtler,a Yrene Díaz,a Helfried Neumann,b Matthias Beller,b Matthieu Nicoul,c Dominik Schaniel,c Axel G. Griesbeck,*,a Axel Jacobi von Wangelin*,a.

a Department of Chemistry, University of Cologne, Germany, Greinsstrasse, 4, 50939, Köln; Fax: +49 (0) 2214705057

E-mail: ,

b Department of Physics I, University of Cologne, Germany.

c Leibniz-Institute of Catalysis, University of Rostock, Germany.

S1: This page.

S2: Absorption spectra of 17-24 in DMSO. Concentration was 2.5·10-6 M in all cases.

S3: Normalised absorption, excitation and emission spectra of 17-24.

S4: Absorption spectra of 17-24 in buffer solutions at different pH. Concentration was 1.3·10-3 M in all cases.

S5: Emission spectra of 17-24 in buffer solutions at A) pH = 3; B) pH = 7. Concentration was 2.6·10-6 M in all cases.

S6: Detailed theoretical data for compound 18: (a) vacuum, protonated (spectrum envelope peak half-width at half height 0.18 eV)

S7: Detailed theoretical data for compound 18: (b) vacuum, non-protonated (spectrum envelope peak half-width at half height 0.18 eV)

S8: Detailed theoretical data for compound 18: (c) in DMSO, protonated (spectrum envelope peak half-width at half height 0.18 eV)

S9: Detailed theoretical data for compound 18: (d) in DMSO, non-protonated (spectrum envelope peak half-width at half height 0.18 eV)


Detailed theoretical data for compound 18:

(a) vacuum, protonated (spectrum envelope peak half-width at half height 0.18 eV)

Method: RB3LYP/6-311++G(d,p) E(RB+HF-LYP) = -702.967066970 a.u.

Zero-point correction= 0.216770 (Hartree/Particle)

Thermal correction to Energy= 0.230322

Sum of electronic and zero-point Energies= -702.750297

Sum of electronic and thermal Energies= -702.736745

Number of imaginary frequencies = 0

Excited State 1: Singlet-A 3.9384 eV 314.81 nm f=0.0421

Excited State 2: Singlet-A 4.1572 eV 298.24 nm f=0.0065

Excited State 3: Singlet-A 4.3636 eV 284.13 nm f=0.0185

Excited State 4: Singlet-A 4.5815 eV 270.62 nm f=0.0159

Excited State 5: Singlet-A 4.6921 eV 264.24 nm f=0.0242

Excited State 6: Singlet-A 5.0566 eV 245.19 nm f=0.0240

Excited State 7: Singlet-A 5.1273 eV 241.81 nm f=0.0000

Excited State 8: Singlet-A 5.4702 eV 226.65 nm f=0.0004

Excited State 9: Singlet-A 5.6031 eV 221.28 nm f=0.2743

Excited State 10: Singlet-A 5.8040 eV 213.62 nm f=0.5247


(b) vacuum, non-protonated (spectrum envelope peak half-width at half height 0.18 eV)

Method: RB3LYP/6-311++G(d,p) E(RB+HF-LYP) = -702.607387683 a.u.

Zero-point correction= 0.204601 (Hartree/Particle)

Thermal correction to Energy= 0.218158

Sum of electronic and zero-point Energies= -702.402787

Sum of electronic and thermal Energies= -702.389230

Number of imaginary frequencies = 0

Excited State 1: Singlet-A 3.5272 eV 351.51 nm f=0.1489

Excited State 2: Singlet-A 4.2140 eV 294.22 nm f=0.0055

Excited State 3: Singlet-A 4.3163 eV 287.25 nm f=0.0337

Excited State 4: Singlet-A 4.4701 eV 277.36 nm f=0.0027

Excited State 5: Singlet-A 4.6968 eV 263.98 nm f=0.0008

Excited State 6: Singlet-A 4.7326 eV 261.98 nm f=0.0054

Excited State 7: Singlet-A 4.9210 eV 251.95 nm f=0.0009

Excited State 8: Singlet-A 4.9744 eV 249.24 nm f=0.0062

Excited State 9: Singlet-A 5.2103 eV 237.96 nm f=0.1746

Excited State 10: Singlet-A 5.3007 eV 233.90 nm f=0.0073


(c) in DMSO, protonated (spectrum envelope peak half-width at half height 0.18 eV)

Method: RB3LYP/6-311++G(d,p) E(RB+HF-LYP) = -703.062951316 a.u.

Zero-point correction= 0.218286 (Hartree/Particle)

Thermal correction to Energy= 0.231786

Sum of electronic and zero-point Energies= -702.844666

Sum of electronic and thermal Energies= -702.831166

Number of imaginary frequencies = 0

Excited State 1: Singlet-A 4.0751 eV 304.25 nm f=0.0787

Excited State 2: Singlet-A 4.3615 eV 284.27 nm f=0.0407

Excited State 3: Singlet-A 4.5574 eV 272.05 nm f=0.0040

Excited State 4: Singlet-A 4.6501 eV 266.62 nm f=0.0340

Excited State 5: Singlet-A 5.0458 eV 245.72 nm f=0.0071

Excited State 6: Singlet-A 5.0587 eV 245.09 nm f=0.0022

Excited State 7: Singlet-A 5.0793 eV 244.10 nm f=0.0785

Excited State 8: Singlet-A 5.5277 eV 224.30 nm f=0.3901

Excited State 9: Singlet-A 5.6510 eV 219.40 nm f=0.7289

Excited State 10: Singlet-A 5.7980 eV 213.84 nm f=0.0000


(d) in DMSO, non-protonated (spectrum envelope peak half-width at half height 0.18 eV)

Method: RB3LYP/6-311++G(d,p) RB3LYP/6-311++G(d,p) E(RB+HF-LYP) = -702.627646819 a.u.

Zero-point correction= 0.204046 (Hartree/Particle)

Thermal correction to Energy= 0.217526

Sum of electronic and zero-point Energies= -702.423601

Sum of electronic and thermal Energies= -702.410121

Number of imaginary frequencies = 0

Excited State 1: Singlet-A 3.5259 eV 351.64 nm f=0.2050

Excited State 2: Singlet-A 4.2399 eV 292.42 nm f=0.0722

Excited State 3: Singlet-A 4.2848 eV 289.36 nm f=0.0072

Excited State 4: Singlet-A 4.6732 eV 265.31 nm f=0.0011

Excited State 5: Singlet-A 4.8709 eV 254.54 nm f=0.0006

Excited State 6: Singlet-A 4.9598 eV 249.98 nm f=0.0068

Excited State 7: Singlet-A 4.9823 eV 248.85 nm f=0.0017

Excited State 8: Singlet-A 5.1652 eV 240.04 nm f=0.3382

Excited State 9: Singlet-A 5.2880 eV 234.47 nm f=0.0043

Excited State 10: Singlet-A 5.4459 eV 227.66 nm f=0.0122

Raúl Pérez-Ruiz et al. J Fluorescence -9-