Supplementary material

Supplementary data are available at JXB online.

Suppl. Fig. S1 Nucleotide and corresponding encoded amino acid sequence alignment of two uORFs

Suppl. Fig. S2 Multiple alignments of the deduced amino acid sequences of CaSAMDC and other plant SAMDCs

Suppl. Fig. S3 Construct map of 35S::CaSAMDC showing transcription units and relevant restriction sites

Suppl. Fig. S4 Southern blot analysis of CaSAMDC in genomic DNA from WT and independent transgenic lines #2 and #4 containing 35S::CaSAMDC

Suppl. Fig. S5 Patterns of transcription and PA biosynthesis in response to abiotic stresses in aerial parts of hot pepper (Capsicum annum) and transgenic Arabidopsis plants overexpressing CaSAMDC

Suppl. Fig. S6 Phenotypic analyses of WT and CaSMADC-overexpressing transgenic plants in response to drought stress

Suppl. Table S1. Sequences of primers for RT-PCR

Suppl. Table S1. Sequences of primers for RT-PCR

Gene / Strand / Sequences

Semiquantitative RT-PCR

CaActin / Forward
Reverse / TTGGACTCTGGTGATGGTGTG
AACATGGTTGAGCCACCACTG
AtUBC10 / Forward
Reverse / TGGATATGGCGTCGAAGC
GTGGGATTTTCCATTTAGCC
CaDhn / Forward
Reverse / TGGCTGATCAGTATGAACACAAC
TGATACCCTGGCAATTTCTC
CaLEAL-1 / Forward
Reverse / TGACAAGGCGAAGAATTTTGTATC CATATCTCTACAACCTTTGATGGA
CaSAMDC / Forward
Reverse / CTGCTGCTGAGATGACTGAACT
TACCAGAGCTATAGTATTGCGTAC
SAMDC1 / Forward / ACTCCGTGCTCTGACCAAGT
Reverse / GCTGCTCCATTCACACTTCA
SAMDC2 / Forward / AGCTCTGCTGGTTCTATGACT
Reverse / CATTTGGATCATCCGAATTC
ADC1 / Forward / CTGCGCAGCTTTACTGTGAG
Reverse / CGGCTCGGTGTTTAAGAGTC
ADC2 / Forward / CTTCCGGTGGTTTAGGATTG
Reverse / GCGCCTATAGCCTTCAACAC

Real-time qRT-PCR

AtACT2 / Forward
Reverse / GAATGGAAGCTGCTGGAATC
CAGCGATGCCTGAGAACATA
AtDAO1 / Forward / ATCCTCCCGCATCTACACAG
Reverse / TCGGAGATGTTTAGGGATCG
AtPAO1 / Forward / CCAGGAGACGATGAAAGAGG
Reverse / GTAGCTACCGCGTTGAAACC
AtPAO2 / Forward / GGCCACAGGGTTACTAAGATTG
Reverse / AAACTCCAAGAGGGACAGCA
AtPAO3 / Forward / ATTTCAAGGCGCTACAGTGG
Reverse / ACTGTGGAAGCTTTGGTTCG
AtPAO4 / Forward / GAACAATGTGCAGCCTTCTG
Reverse / ATGGATACGACCACCAATCC
AtPAO5 / Forward / ACTGCCACTAGGGTTTGCTC
Reverse / TCCTTCTTCTCCTCCATTGC
RbohD / Forward
Reverse / ACACATGACGAAGCCACAAG
ACGGTCCTGAGCTTACGTGT
RbohF / Forward
Reverse / TCGTCTCCAGATCTTCTTCG
GTTGCCATAGCTCTATGTAGCC
MnSODmi / Forward
Reverse / CAGAAGCATCACCAGGCTTA
CGAATGGTTGACATGACCTC
CAT1 / Forward / GTCGGATGAGGAAGCTATCAG
Reverse / TCCATCACCTGAACGAAGAG
AtMC4 / Forward / GCCTACTGGCAAGAACATCC
Reverse / TCGAAACCAGTGTCATCGTC
AtMC8 / Forward / GACCCTACAGGGTTTGACGA
Reverse / GGATGAGACCACCACTATGACA