Supplemental Figure 4. Bioinformatic comparison of CLSY1 with other SNF2 domain-containing proteins
A) Alignment of the SNF2 and helicase (HelicC) motifs of CLSY1 with 38 homologues from the Arabidopsis genome. The consensus sequence is shown at a 70 % cut off for similar (not necessarily identical) residues. Red type on a yellow background indicates the residue is conserved in all sequences. Blue highlighting indicates the consensus residue occurs in at least 70 % of the aligned sequences while green highlighting indicates similar residues occur in at least 70 % of the aligned sequences. Green type indicates that there is weak biochemical similarity between the indicated amino acid and the consensus residue. An asterisk below the consensus sequence indicates a residue conserved in 70 % of Arabidopsis SNF2 domain-containing proteins but not within the DRD1/CLSY1 family. Underlining below the consensus sequence indicates the nine conserved amino acid blocks identified in Shaked et al. (2006).
B) AlignX alignment of the plant-specific DRD1/CLSY1 SNF2 subfamily. Aqua indicates residues conserved in 50 % of sequences, while similar residues are highlighted in green with amino acids of lower similarity given in green type. A yellow background with red type indicates identical residues across all sequences. An asterisk above the amino acid sequence indicates the start of the SNF2 domain.
C) Phylogenetic tree of Arabidopsis SNF2 domain-containing proteins.
Helicase C and SNF2 domain phylogenetic trees with the Arabidopsis SNF2 domain-containing proteins indicated by their AGI identifier. The subfamily containing CLSY1 and DRD1 is circled. Large letters on the right indicate the SNF2 subfamilies. Non-Arabidopsis homologues are indicated as below.
Mouse: mSNF2L, mCHD1, mATRX
Drosophila: ISWI, BRM
C. elegans: ISW-1
H. sapiens: CHD4, hINO80, hATRX, ERCC6, hBRG1
S. cerevisiae: CHD1, RAD54, RAD26, RAD 5, STH1, Snf2
S. pombe: RAD8
D) AlignX alignment of CLSY1 homologues from plant species. Homologues were identified through a protein query-translated database (tblastn) search using the Basic Local Alignment Search Tool (BLAST) algorithm at NCBI with the default settings (M. truncatula and O. pumila) and through the ChromDB database (O. sativa). A. thaliana = Arabidopsis thaliana; O. pumila = Olimarabidopsis pumila; M. truncatula = Medicago truncatula; O. sativa = Oryza sativa. Aqua indicates residues conserved in at least 50 % of sequences, similar residues are highlighted in green with amino acids of lower similarity given in green type. A yellow background with red type indicates identical residues across all sequences. A red line above the alignment indicates the predicted ‘AT hook’ motif, while a green line delineates the predicted NLS for AtCLSY1. Regions with good sequence conservation in the N-terminal domain are indicated with a dashed line, and the C-terminal SNF2 and HelicC domains are marked by black lines.
Supplemental Figure S42A
1 50
At1g02670 SNF2 (1) YQKEFLAWATIQELS------A------VRGGIL
At1g05120 SNF2 (1) YQKEFLAWATKQEQ------S------VAGGIL
At1g11100 SNF2 (1) HQRIALSWMSQKETSG------NP------CFGGIL
At1g61140 SNF2 (1) HQRIALSWMAQKETSG------FP------CSGGIL
At1g50410 SNF2 (1) HQKIALAWMFQKETNS------LH------CMGGIL
At3g20010 SNF2 (1) HQKIALAWMFQKETSS------FN------CPGGIL
At3g16600 SNF2 (1) HQKIALNWMRKKEKRS------RH------CLGGIL
At5g05130 SNF2 (1) HQKEGLGWLLHREKSGELPPFWEEKDGEFLNTLTNYRSDKRPDPLRGGVF
At5g22750 SNF2 (1) YQKQALHWMTQLEKGNCTDEAATML...SFTGDATIHFPSTLQMARGGIL
At5g43530 SNF2 (1) YQKQALYWMSESEKGIDVEKAAETL...IFSGEATIQFPTATQMARGGIL
At2g40770 SNF2 (1) ------
At3g54460 SNF2 (1) ------
At1g48310 SNF2 (1) FQREGIEFILQHGG------R------VLL
At5g07810 SNF2 (1) FQLDGLRFGLRRGG------R------CFI
At5g63950 SNF2 (1) HQREGLNWLWSLHTQG------KG------GIL
At1g03750 SNF2 (1) HQREGVKFMYNLYKNN------HGGIL
At2g18760 SNF2 (1) YQRVGVQWLWELHCQR------AG------GII
At1g08600 SNF2 (1) HQVTGIRFMWENIIQS------ISRVKSG-----DKGLGCIL
At2g44980 SNF2 (1) HQVEGVSWLIQKYLLG------VN------VVLE
At3g54280 SNF2 (1) YQQEGINWLGFLKR------FK------LHGIL
At2g02090 SNF2 (1) YQLVGVNFLLLLYKKG------IEG------AIL
At3g12810 SNF2 (1) YQHIGLDWLVTMYEKK------LN------GIL
At2g13370 SNF2 (1) YQLEGLNFLVNSWLND------TN------VIL
At3g06400 SNf2 (1) YQLAGLNWLIRLYENG------IN------GIL
At5g18620 SNF2 (1) YQLAGLNWLIRLYENG------IN------GIL
At4g31900 SNF2 (1) YQLEGLNFLRYSWSKK------TN------VIL
At5g44800 SNF2 (1) HQLEALNWLRRCWHKS------KN------VIL
At2g28290 SNF2 (1) YQMNGLRWLVSLYNNH------LN------GIL
At3g06010 SNF2 (1) YQLEGLQWMVSLFNNN------LN------GIL
At3g19310 SNF2 (1) YQLEGLQWMVSLYNND------YN------GIL
At2g46020 SNF2 (1) YQLVGLQWMLSLYNNK------LN------GIL
At3g57300 SNF2 (1) YQMKGLQWLVNCYEQG------LN------GIL
At5g66750 SNF2 (1) YQLKGVKWLISLWQNG------LN------GIL
At1g05480 SNF2 (1) HQQEGFEFIWKNLAGT------IMLNELKDFENSDETGGCIM
At3g24340 SNF2 (1) HQQEGFEFIWKNLAGT------TKINELNSVGVKGS-GGCII
At2g21450 SNF2 (1) HQTEGFRFLCNNLAAD------EP------GGCIL
At2g16390 SNF2 (1) HQIEGFQFLCSNLVAD------DP------GGCIM
At5g20420 SNF2 (1) HQRRAFEFLWRNVAGS------VEPSLMDPTSGNIG--GCVI
At3g42670 SNF2 (1) HQKKAFEFLWKNLAGS------VVPAMMDPSSDKIG---CVV
Consensus (1) HQ GL WL IL
* note: conserved amino acid region identified
in Shaked et al. 2006 extends 3 residues in
the N-terminal direction
51 100
At1g02670 SNF2 (23) ADEMGMGKTIQAISLVLARREVDRAK------
At1g05120 SNF2 (22) ADEMGMGKTIQAISLVLARREVDRAQ------
At1g11100 SNF2 (25) ADDQGLGKTVSTIALILTERSTPYLPCEEDSK------
At1g61140 SNF2 (25) ADDQGLGKTVSTIALILKERSKPAQACEESTKKEIFDLESETGECAPLKP
At1g50410 SNF2 (25) ADDQGLGKTVSTIALILKQMHEAKLKSKNSGNQEAEALDLDADDESENAF
At3g20010 SNF2 (25) ADDQGLGKTVSTIALILKQKIVSQLKSESSCKQETEALVLDADDESDNAK
At3g16600 SNF2 (25) ADDQGLGKTISTISLILLQKLKSQSK------
At5g05130 SNF2 (51) ADDMGLGKTLTLLSLIAFDRYGNASTSTPTEEPLDGEGDKIE------
At5g22750 SNF2 (6) ADAMGLGKTVMTISLLLAHSWKAASTGFLCPNYE------
At5g43530 SNF2 (6) ADAMGLGKTVMTIALILARPGRGN------PENE------
At2g40770 SNF2 (1) ------
At3g54460 SNF2 (1) ------
At1g48310 SNF2 (19) ADEMGLGKTLQAIAVTTCVQES------
At5g07810 SNF2 (4) ADEMGLGKTLQAIAIAGCFISEGS------
At5g63950 SNF2 (22) GDDMGLGKTMQICSFLAGLFHSKLIKR------
At1g03750 SNF2 (22) GDDMGLGKTIQTIAFLAAVYGKDGDAG------
At2g18760 SNF2 (22) GDEMGLGKTIQVLSFLGSLHFSKMYK------
At1g08600 SNF2 (32) AHTMGLGKTFQVIAFLYTAMRCVDLGL------
At2g44980 SNF2 (23) LDQMGLGKTLQAISFLSYLKFRQGLPG------
At3g54280 SNF2 (22) CDDMGLGKTLQASAIVASDAAERRGS------
At2g02090 SNF2 (23) ADEMGLGKTIQAITYLTLLSRLNNDPG------
At3g12810 SNF2 (22) ADEMGLGKTIMTIALLAHLACDKGIWG------
At2g13370 SNF2 (22) ADEMGLGKTVQSVSMLGFLQNTQQIPG------
At3g06400 SNf2 (22) ADEMGLGKTLQTISLLAYLHEYRGING------
At5g18620 SNF2 (22) ADEMGLGKTLQTISLLAYLHEYRGING------
At4g31900 SNF2 (22) ADEMGLGKTIQSIAFLASLFEENLS------
At5g44800 SNF2 (22) ADEMGLGKTVSASAFLSSLYFEFGVAR------
At2g28290 SNF2 (22) ADEMGLGKTVQVISLICYLMETKNDRG------
At3g06010 SNF2 (22) ADEMGLGKTIQTISLIAYLLENKGVPG------
At3g19310 SNF2 (22) ADEMGLGKTIQTIALIAYLLESKDLHG------
At2g46020 SNF2 (22) ADEMGLGKTVQVMALIAYLMEFKGNYG------
At3g57300 SNF2 (22) ADEMGLGKTIQAMAFLAHLAEEKNIWG------
At5g66750 SNF2 (22) ADQMGLGKTIQTIGFLSHLKGN-GLDG------
At1g05480 SNF2 (37) SHAPGTGKTRLTIIFLQAYLQCFPD------
At3g24340 SNF2 (36) SHKAGTGKTRLTVVFLQSYLKRFPN------
At2g21450 SNF2 (24) AHAPGSGKTFLLISFLQSFMAMDPQ------
At2g16390 SNF2 (24) AHAPGSGKTFMIISFMQSFLAKYPQ------
At5g20420 SNF2 (35) SHSPGAGKTFLIIAFLTSYLKLFPG------
At3g42670 SNF2 (4) SHTPGAGKTFLIIAFLASYLKIFPG------
Consensus (51) AD GLGKTI IA L
* * *
101 150
At1g02670 SNF2 (49) ------SRE------
At1g05120 SNF2 (48) ------FGE------
At1g11100 SNF2 (57) ------NGGCNQSDHSQVVFNENKVVED-----
At1g61140 SNF2 (75) ------SGRSKHFEHSQLLSNENKVGGD-----
At1g50410 SNF2 (75) E------KPESKASN------GSGVNGDSGIKKAKGEEAST-----
At3g20010 SNF2 (75) HESGSHVKPELKVSSNSETSVLSACGNDENDSSDMEKAEDEEANS-----
At3g16600 SNF2 (51) ------QR--KRKGQN------
At5g05130 SNF2 (93) ------KKGKKRGRGKSSES---VTRKK
At5g22750 SNF2 (40) ------GDKVISSSVDDLTSPPVKATKFLGF
At5g43530 SNF2 (34) ------DVLVADVNADKRNRK------E--
At2g40770 SNF2 (1) ------
At3g54460 SNF2 (1) ------
At1g48310 SNF2 (41) ------
At5g07810 SNF2 (28) ------
At5g63950 SNF2 (49) ------
At1g03750 SNF2 (49) ------
At2g18760 SNF2 (48) ------
At1g08600 SNF2 (59) ------
At2g44980 SNF2 (50) ------
At3g54280 SNF2 (48) ------
At2g02090 SNF2 (50) ------
At3g12810 SNF2 (49) ------
At2g13370 SNF2 (49) ------
At3g06400 SNf2 (49) ------
At5g18620 SNF2 (49) ------
At4g31900 SNF2 (47) ------
At5g44800 SNF2 (49) ------
At2g28290 SNF2 (49) ------
At3g06010 SNF2 (49) ------
At3g19310 SNF2 (49) ------
At2g46020 SNF2 (49) ------
At3g57300 SNF2 (49) ------
At5g66750 SNF2 (48) ------
At1g05480 SNF2 (62) ------
At3g24340 SNF2 (61) ------
At2g21450 SNF2 (49) ------
At2g16390 SNF2 (49) ------
At5g20420 SNF2 (60) ------
At3g42670 SNF2 (29) ------
Consensus (101)
151 200
At1g02670 SNF2 (52) ------AVGHTLVLVPPVALSQWLDEISRLTSPG-STRVLQYHGP
At1g05120 SNF2 (51) ------AAGCTLVLCPLVAVSQWLNEIARFTSPG-STKVLVYHGA
At1g11100 SNF2 (79) --SLCKMRG-RPAAGTLIVCPTSLMRQWADELRKKVTLEAHLSVLVYHGC
At1g61140 SNF2 (97) --SVGKVTG-RPAAGTLVVCPTSVMRQWADELHKKVTSEANLSVLVYHGS
At1g50410 SNF2 (104) --STRKFNRKRPAAGTLIVCPASVVRQWARELDEKVTDEAKLSVLIYHGG
At3g20010 SNF2 (120) --STRAFQWKRPAAGTLIVCPASVVRQWARELDEKVSEESKLSVLVYHGS
At3g16600 SNF2 (59) ------SGGTLIVCPASVVKQWAREVKEKVSDEHKLSVLVHHGS
At5g05130 SNF2 (112) LKTDDVVGMNVSQKTTLIVCPPSVISAWITQLEEHTVPG-ILKVYMYHGG
At5g22750 SNF2 (65) DKRLLEQKSVLQNGGNLIVCPMTLLGQWKTEIEMHAKPG-SLSVYVHYGQ
At5g43530 SNF2 (50) --IHMALTTVKAKGGTLIICPMALLSQWKDELETHSKPD-TVSVLVYYGG
At2g40770 SNF2 (1) ------TGATLIVCPAPILPQWHSEITRHTRLG-SLITCIYEGV
At3g54460 SNF2 (1) ------TLIVVPTNLVNHWLTQIQKHVCSD-QLRILVWADH
At1g48310 SNF2 (41) ------WPVLIIAPSSLRLHWATMIHQWLHVPPSDIVVVLPQP
At5g07810 SNF2 (28) ------ILVVCPAVLRFTWAEELERWLPSCLPSDVHLVFGH
At5g63950 SNF2 (49) ------ALVVAPKTLLPHWMKELATVGLSQ---MTREYYGT
At1g03750 SNF2 (49) ----ESCLLESDKGPVLIICPSSIIHNWESEFSRWASFF---KVSVYHGS
At2g18760 SNF2 (48) ------PSIIICPVTLLRQWRREAQKWYPDF---HVEILHDS
At1g08600 SNF2 (59) ------KTALIVTPVNVLHNWRSEFEKWMP-----SEVKPLRI
At2g44980 SNF2 (50) ------PFLVLCPLSVTDGWVSEINRFTPNL---EVLRYVGD
At3g54280 SNF2 (48) ------TDELDVFPSIIVCPSTLVGHWAFEIEKYIDLS-LLSVLQYVGS
At2g02090 SNF2 (50) ------PHLVVCPASVLENWERELRKWCPSF---TVLQYHGA
At3g12810 SNF2 (49) ------PHLIVVPTSVMLNWETEFLKWCPAF---KILTYFGS
At2g13370 SNF2 (49) ------PFLVVVPLSTLANWAKEFRKWLPGM---NIIVYVGT
At3g06400 SNf2 (49) ------PHMVVAPKSTLGNWMNEIRRFCPVL---RAVKFLGN
At5g18620 SNF2 (49) ------PHMVVAPKSTLGNWMNEIRRFCPVL---RAVKFLGN
At4g31900 SNF2 (47) ------PHLVVAPLSTIRNWEREFATWAPHM---NVVMYTGD
At5g44800 SNF2 (49) ------PCLVLVPLSTMPNWLSEFSLWAPLL---NVVEYHGS
At2g28290 SNF2 (49) ------PFLVVVPSSVLPGWQSEINFWAPSI---HKIVYCGT
At3g06010 SNF2 (49) ------PYLIVAPKAVLPNWVNEFATWVPSI---AAFLYDGR
At3g19310 SNF2 (49) ------PHLILAPKAVLPNWENEFALWAPSI---SAFLYDGS
At2g46020 SNF2 (49) ------PHLIIVPNAVLVNWKSELHTWLPSV---SCIYYVGT
At3g57300 SNF2 (49) ------PFLVVAPASVLNNWADEISRFCPDL---KTLPYWGG
At5g66750 SNF2 (48) ------PYLVIAPLSTLSNWFNEIARFTPSI---NAIIYHGD
At1g05480 SNF2 (62) ------CKPVIIAPASLLLTWAEEFKKWNIS----IPFHNLSS
At3g24340 SNF2 (61) ------SHPMVIAPATLMRTWEDEVRKWNVN----IPFYNMNS
At2g21450 SNF2 (49) ------ARPLVVLPKGIIESWKREFTLWEVEK---IPLLDFYS
At2g16390 SNF2 (49) ------AKPLVVLPKGILPTWKKEFVRWQVED---IPLLDFYS
At5g20420 SNF2 (60) ------KRPLVLAPKTTLYTWYKEFIKWEIP----VPVHLIHG
At3g42670 SNF2 (29) ------KRPLVLAPKTTLYTWYKEFIKWEIP----VPVHLLHG
Consensus (151) LVV P VL W E L G
*
201 250
At1g02670 SNF2 (90) ------KRD
At1g05120 SNF2 (89) ------KRA
At1g11100 SNF2 (126) ------SRT
At1g61140 SNF2 (144) ------SRT
At1g50410 SNF2 (152) ------NRT
At3g20010 SNF2 (168) ------NRT
At3g16600 SNF2 (97) ------HRT
At5g05130 SNF2 (161) ER------T
At5g22750 SNF2 (114) ------SRP
At5g43530 SNF2 (97) ------DRT
At2g40770 SNF2 (38) RNASLSEEP------M
At3g54460 SNF2 (35) IELS------
At1g48310 SNF2 (78) GGSNKCGFTIVSSN------
At5g07810 SNF2 (63) QDNPAYLPR------
At5g63950 SNF2 (81) STKAREYDLHHILQ------
At1g03750 SNF2 (92) NRDMILEKLKAR------
At2g18760 SNF2 (81) AQDSGHGKGQGKAS------ESDYDSESSVDSDHEPKSKNTKKWDSL
At1g08600 SNF2 (91) FMLGDVSR------ERRFD
At2g44980 SNF2 (83) KYCRLDMRKSMYDHG------
At3g54280 SNF2 (90) AQDRVSLREQFN------
At2g02090 SNF2 (83) ARAAYSRELNSLSKAG------
At3g12810 SNF2 (82) AKERKLKRQGWMK------
At2g13370 SNF2 (82) RAS----REVRNKTND------VHK---VGR--
At3g06400 SNf2 (82) PEERRHIREDLLVA------
At5g18620 SNF2 (82) PEERRHIREELLVA------
At4g31900 SNF2 (80) SEARDVIWEHEFYFSE------GRKS--
At5g44800 SNF2 (82) AKGRAIIRDYEWHAKN------STGTTKKP
At2g28290 SNF2 (82) PDERRKLFKEQIVH------
At3g06010 SNF2 (82) LEER-KAIREKIAGE------
At3g19310 SNF2 (82) KEKR-TEIRARIAG------
At2g46020 SNF2 (82) KDQR-SKLFSQVKF------
At3g57300 SNF2 (82) LQERTILRKNINPKRM------YRR------
At5g66750 SNF2 (81) KNQR-DELRRKHMPKT------VG------
At1g05480 SNF2 (95) LDFTGKENSAALGLLMQKNATA------RSNNEIRMV
At3g24340 SNF2 (94) LQLSGYEDAEAVSRLEGN------RHHNSIRMV
At2g21450 SNF2 (83) VKAESRKQQ------LK
At2g16390 SNF2 (83) AKAENRAQQ------LS
At5g20420 SNF2 (93) RRTYCTFKQNKTVQFNGVPKPSR------DVMHVLDCLE
At3g42670 SNF2 (62) RRTYCMSKE-KTIQFEGIPKPSQ------DVMHVLDCLD
Consensus (201)
251 300
At1g02670 SNF2 (93) KNVQKLMNYDFVLTTSPIVENEYRK------
At1g05120 SNF2 (92) KNIKEFMNYDFVLTTYSTVESEYRRNIMPSKVQCAYCSKSFYPKKLVIHL
At1g11100 SNF2 (129) KDPHELAKYDVVITTYSLVS------
At1g61140 SNF2 (147) KDPHELAKYDVVVTTFSIVSMEVPKQPLVDDEDEEKDGVHDGGTAAT---
At1g50410 SNF2 (155) KDPIELAKYDVVMTTYAIVSNEVPKQPLVDDDENDEKNSEKYGLAS----
At3g20010 SNF2 (171) KDPNELAEYDVVVTTYAIVTNEAPNKFLVDEDENDEKNTDRYGLAS----
At3g16600 SNF2 (100) KDPTEIAIYDVVMTTYAIVTNEVPQNPMLNRYDSMRG------
At5g05130 SNF2 (164) DDVNELMKYDIVLTTYGTLAVEES------
At5g22750 SNF2 (117) KDAKLLSQSDVVITTYGVLTSEFSQEN------
At5g43530 SNF2 (100) HDAKAIASHDVVLTTYGVLTSAYKQD------
At2g40770 SNF2 (48) IDITELLNADIVLTTYDVLKEDLTHDFDRHDG------
At3g54460 SNF2 (39) -PHSLAWDYDVVITTFSRLSAEWNPR------
At1g48310 SNF2 (92) TKGTIHLDGVFNIVSYDVVTK------
At5g07810 SNF2 (72) ------WPKVVVISYKMLQHLR------
At5g63950 SNF2 (95) ------GKGILLTTYDIVRNNTKALQG------
At1g03750 SNF2 (104) ------GVEVLVTSFDTFRIQG------
At2g18760 SNF2 (122) LNRVLNSESGLLITTYEQLRLQ------
At1g08600 SNF2 (104) LLTKWRKKGGVFLMGYTNFRNLSLGRGVKDLN------
At2g44980 SNF2 (98) ----HFLPFDVLLTTYDIALV------
At3g54280 SNF2 (102) ------NHNVIITSYDVVRK------
At2g02090 SNF2 (99) ----KPPPFNVLLVCYSLFERHSEQQK------
At3g12810 SNF2 (95) -----LNSFHVCITTYRLVIQ------
At2g13370 SNF2 (100) -----PIKFNALLTTYEVVLK------
At3g06400 SNf2 (96) ------GKFDICVTSFEMAIK------
At5g18620 SNF2 (96) ------GKFDICVTSFEMAIK------
At4g31900 SNF2 (100) ------KFDVLLTTYEMVHP------
At5g44800 SNF2 (106) TS----YKFNVLLTTYEMVLA------
At2g28290 SNF2 (96) ------QKFNVLLTTYEYLMNKH------
At3g06010 SNF2 (96) ------GKFNVLITHYDLIMR------
At3g19310 SNF2 (95) ------GKFNVLITHYDLIMR------
At2g46020 SNF2 (95) ------EKFNVLVTTYEFIM--Y------
At3g57300 SNF2 (101) -----DAGFHILITSYQLLVT------
At5g66750 SNF2 (98) ------PKFPIVITSYEVAMN-D------
At1g05480 SNF2 (126) KIYSWIKSKSILGISYNLYEKLAGVKDEDKKTKMVREVKPD------
At3g24340 SNF2 (121) KLVSWWKQKSILGISYPLYEKLAANKNTEG---MQ------
At2g21450 SNF2 (94) VLGQWIKERSILFLGYQQFTRIICDDNFEAASED------
At2g16390 SNF2 (94) ILKQWMEKKSILFLGYQQFSTIVCDD--TTDSLS------
At5g20420 SNF2 (126) KIQKWHAHPSVLVMGYTSFTTLMREDSKFAHR------
At3g42670 SNF2 (94) KIQKWHAQPSVLVMGYTSFLTLMREDSKFAHR------
Consensus (251) VLLTTY
*
301 350
At1g02670 SNF2 (118) ------DEGVDETMSP------
At1g05120 SNF2 (142) RYFCGPSAVKTAKQSKQKRKKTSDSSSQQGKEADAGEDKKLKKSKKKTKQ
At1g11100 SNF2 (149) ------KRKHMDCEPVE------
At1g61140 SNF2 (194) ------GFCSNKKRKYPPDS------KKKGSKKKKVE------
At1g50410 SNF2 (201) ------GFSINKKRKNVVGTTKKSKKKKGNNNAGDSSD------
At3g20010 SNF2 (217) ------GFSNNKKRKVVVGASKKSKRRGRKSTNDTSSE------
At3g16600 SNF2 (137) ------RE--S------LDGSSLIQ------
At5g05130 SNF2 (188) ------
At5g22750 SNF2 (144) ------S------
At5g43530 SNF2 (126) ------
At2g40770 SNF2 (80) ------D
At3g54460 SNF2 (64) ------
At1g48310 SNF2 (113) ------
At5g07810 SNF2 (88) ------
At5g63950 SNF2 (116) ------
At1g03750 SNF2 (120) ------
At2g18760 SNF2 (144) ------
At1g08600 SNF2 (136) ------
At2g44980 SNF2 (115) ------
At3g54280 SNF2 (116) ------
At2g02090 SNF2 (122) ------
At3g12810 SNF2 (111) ------
At2g13370 SNF2 (116) ------
At3g06400 SNf2 (111) ------
At5g18620 SNF2 (111) ------
At4g31900 SNF2 (114) ------
At5g44800 SNF2 (123) ------
At2g28290 SNF2 (113) ------
At3g06010 SNF2 (111) ------
At3g19310 SNF2 (110) ------
At2g46020 SNF2 (110) ------
At3g57300 SNF2 (117) ------
At5g66750 SNF2 (114) ------
At1g05480 SNF2 (167) ------
At3g24340 SNF2 (153) ------
At2g21450 SNF2 (128) ------
At2g16390 SNF2 (126) ------
At5g20420 SNF2 (158) ------
At3g42670 SNF2 (126) ------
Consensus (301)
351 400
At1g02670 SNF2 (128) ------LHSIKWNRIIVDEAHDIKNRSS----RTAKAVF-
At1g05120 SNF2 (192) TVEKDQLGSDDKEKSLLHSVKWNRIILDEAHYIKERRS----NTARAVF-
At1g11100 SNF2 (160) ------FLSGPLAQVSWFRVVLDEAQSIKNYKT----QASIACS-
At1g61140 SNF2 (219) ------FLSGPLAKVSWFRVVLDEAQSIKNYKT----QVARACW-
At1g50410 SNF2 (233) ------PDSGTLAKVGWFRVVLDEAQTIKNHRT----QVARACC-
At3g20010 SNF2 (249) ------PDCGPLGKVGWFRIVLDEAQTIKNYRT----QMARSCC-
At3g16600 SNF2 (148) ------PHVGALGRVRWLRVVLDEAHTIKNHRT----LIAKACF-
At5g05130 SNF2 (188) ------WEDSPVKKMEWLRIILDEAHTIKNANA----QQSRVVC-
At5g22750 SNF2 (145) ------ADHEGIYAVRWFRIVLDEAHTIKNSKS----QISLAAA-
At5g43530 SNF2 (126) ------MANSIFHRIDWYRIVLDEAHTIKSWKT----QAAKATF-
At2g40770 SNF2 (81) RHCLRFQKRYPVIPTPLTRIFWWRICLDEAQMVESNAA----AATEMALR
At3g54460 SNF2 (64) ------KKSPLIQVHWLRVMLDEGHTLGSSVS----LTNKFQMA
At1g48310 SNF2 (113) ------LDKLLMALDFKVVIADESHFLKNGQA----KRTSACLP
At5g07810 SNF2 (88) ------TTMLEREWALLIVDESHHLRCSKKKSDPPEIKTVLD
At5g63950 SNF2 (116) ------DDHYTDEDDEDGNKWDYMILDEGHLIKNPNT----QRAKSLL-
At1g03750 SNF2 (120) ------PVLSGINWEIVIADEAHRLKNEKS----KLYEACL-
At2g18760 SNF2 (144) ------GEKLLNIEWGYAVLDEGHRIRNPNS----DITLVCK-
At1g08600 SNF2 (136) ------AARGICNALRD-GPDILVCDEAHIIKNTKA----DTTQALK-
At2g44980 SNF2 (115) ------DQDFLSQIPWQYAIIDEAQRLKNPNS----VLYNVLLE
At3g54280 SNF2 (116) ------DVDYLTQFSWNYCILDEGHIIKNAKS----KITAAVK-
At2g02090 SNF2 (122) ------DDRKVLKRWRWSCVLMDEAHALKDKNS----YRWKNLMS
At3g12810 SNF2 (111) ------DSKMFKRKKWKYLILDEAHLIKNWKS----QRWQTLL-
At2g13370 SNF2 (116) ------DKAVLSKIKWIYLMVDEAHRLKNSEA----QLYTALL-
At3g06400 SNf2 (111) ------EKTALRRFSWRYIIIDEAHRIKNENS----LLSKTMR-
At5g18620 SNF2 (111) ------EKTTLRRFSWRYIIIDEAHRIKNENS----LLSKTMR-
At4g31900 SNF2 (114) ------GISVLSPIKWTCMIIDEGHRLKNQKS----KLYSSLS-
At5g44800 SNF2 (123) ------DSSHLRGVPWEVLVVDEGHRLKNSES----KLFSLLN-
At2g28290 SNF2 (113) ------DRPKLSKIHWHYIIIDEGHRIKNASC----KLNADLK-
At3g06010 SNF2 (111) ------DKAFLKKIEWYYMIVDEGHRLKNHES----ALAKTLLT
At3g19310 SNF2 (110) ------DKAFLKKIDWNYMIVDEGHRLKNHEC----ALAKTLGT
At2g46020 SNF2 (110) ------DRSKLSKVDWKYIIIDEAQRMKDRES----VLARDLD-
At3g57300 SNF2 (117) ------DEKYFRRVKWQYMVLDEAQAIKSSSS----IRWKTLL-
At5g66750 SNF2 (114) ------AKRILRHYPWKYVVIDEGHRLKNHKC----KLLRELK-
At1g05480 SNF2 (167) ------KELDDIREILMGRPGLLVLDEAHTPRNQRS----CIWKTLS-
At3g24340 SNF2 (153) ------V--FRRMLVELPGLLVLDEGHTPRNQSS----LIWKVLT-
At2g21450 SNF2 (128) ------CKLILLEKPTLLILDEGHTSRNKET----YMLSSLA-
At2g16390 SNF2 (126) ------CQEILLKVPSILILDEGHTPRNEDT----NLLQSLA-
At5g20420 SNF2 (158) ------KYMAKVLRESPGLLVLDEGHNPRSTKS----RLRKALM-
At3g42670 SNF2 (126) ------KYMAKVLRESPGLLVLDEGHNPRSTKS----RLRKALM-
Consensus (351) L W IILDEAH IKN S
* *
401 450
At1g02670 SNF2 (157) -ALEATYRWALSGTPLQND----VDELYSLVSYSFLN------
At1g05120 SNF2 (237) -ALEATYRWALSGTPLQNR----VGELYSLIRFLQIRPYSYYFCKDCDCR
At1g11100 SNF2 (194) -GLHAKRRWCLSGTPIQNS----IADLYSYFRFLKYDP------
At1g61140 SNF2 (253) -GLRAKRRWCLSGTPIQNS----IDDLYSYFRFLKYDP------
At1g50410 SNF2 (267) -GLRAKRRWCLSGTPIQNT----IDDLYSYFRFLKYDP------
At3g20010 SNF2 (283) -TLRAKRRWCLSGTPIQNT----IDDLYSYFRFLRYDP------
At3g16600 SNF2 (182) -SLRAKRRWCLTGTPIKNK----VDDLYSYFRFLRYHP------
At5g05130 SNF2 (222) -KLKASRRWAVTGTPIQNG----SFDLYSLMAFLRFEPFS------
At5g22750 SNF2 (179) -ALVADRRWCLTGTPIQNN----LEDLYSLLRFLRIEP------
At5g43530 SNF2 (160) -ELSSHCRWCLTGTPLQNK----LEDLYSLLCFLHVEP------
At2g40770 SNF2 (127) --LYTKHRWCITGTPIQRK----LDDLFGLLKFLKANPFDVS------
At3g54460 SNF2 (98) VSLTACNRWLLTGTPTPNTPNSQLSHIQPLLKFLHEEVYGE------
At1g48310 SNF2 (147) VIKKAQYAILLSGTPALSR----PIELFKQLEALYPDVYRN------
At5g07810 SNF2 (124) VAEKVKHIILLSGTPSVSR----PFDIFHQINMLWPGLLGKD------
At5g63950 SNF2 (154) -EIPSSHRIIISGTPIQNN----LKELWALFNFSCPGLLGDKN------
At1g03750 SNF2 (151) -EIKTKKRIGLTGTVMQNK----ISELFNLFEWVAPGSLGTR------
At2g18760 SNF2 (176) -QLQTVHRIIMTGAPIQNK----LTELWSLFDFVFPGKLGV------
At1g08600 SNF2 (172) -QVKCQRRIALTGSPLQNN----LMEYYCMVDFVREGFLGS------
At2g44980 SNF2 (149) -QFLIPRRLLITGTPIQNN----LTELWALMHFCMPLVFGT------
At3g54280 SNF2 (149) -QLKAQHRLILSGTPIQNN----IMELWSLFDFLMPGFLGT------
At2g02090 SNF2 (157) VARNANQRLMLTGTPLQND----LHELWSLLEFMLPDIFTTEN------
At3g12810 SNF2 (144) -NFNSKRRILLTGTPLQND----LMELWSLMHFLMPHVFQS------
At2g13370 SNF2 (149) -EFSTKNKLLITGTPLQNS----VEELWALLHFLDPGKFKN------
At3g06400 SNf2 (144) -LFSTNYRLLITGTPLQNN----LHELWALLNFLLPEIFSS------
At5g18620 SNF2 (144) -LFSTNYRLLITGTPLQNN----LHELWALLNFLLPEVFSS------
At4g31900 SNF2 (147) -QFTSKHIVLLTGTPLQNN----LNELFALMHFLDADKFG------
At5g44800 SNF2 (156) -TFSFQHRVLLTGTPLQNN----IGEMYNLLNFLQPSSFPS------
At2g28290 SNF2 (146) -HYVSSHRLLLTGTPLQNN----LEELWALLNFLLPNIFNS------
At3g06010 SNF2 (145) -GYRIKRRLLLTGTPIQNS----LQELWSLLNFLLPHIFNS------
At3g19310 SNF2 (144) -GYRIKRRLLLTGTPIQNS----LQELWSLLNFLLPHIFNS------
At2g46020 SNF2 (143) -RYRCQRRLLLTGTPLQND----LKELWSLLNLLLPDVFDN------
At3g57300 SNF2 (150) -SFNCRNRLLLTGTPIQNN----MAELWALLHFIMPMLFDN------
At5g66750 SNF2 (147) -HLKMDNKLLLTGTPLQNN----LSELWSLLNFILPDIFTS------
At1g05480 SNF2 (204) -KVETQKRILLSGTPFQNN----FLELCNVLGLARPKYLER------
At3g24340 SNF2 (186) -EVRTEKRIFLSGTLFQNN----FKELSNVLCLARPADKDT------
At2g21450 SNF2 (160) -RVKTRRKVVLTGTLFQNN----VEEVFNILDLVRPKFLKRP------
At2g16390 SNF2 (158) -QVQTPRKVVLSGTLYQNH----VKEVFNILNLVRPKFLKLD------
At5g20420 SNF2 (192) -KVGTDLRILLSGTLFQNN----FCEYFNTLCLARPKFIHE------
At3g42670 SNF2 (160) -KVDTDLRILLSGTLFQNN----FCEYFNTLCLARPKFVHE------
Consensus (401) R LTGTPIQN L ELW LL FL
* *
451 500
At1g02670 SNF2 (189) ------FFYSTYASFAFRHTHITFARNVTV------
At1g05120 SNF2 (282) ILDYVAHQSCPHCPHNAVRHFCWWNKYVAKPITVYG------
At1g11100 SNF2 (227) ------YSSYQTFCETIKNPISSY------
At1g61140 SNF2 (286) ------YSSYVLFCSTIKNPITRN------
At1g50410 SNF2 (300) ------YAVYKSFCHQIKGPISRN------
At3g20010 SNF2 (316) ------YAVYKSFYSTIKVPISRN------
At3g16600 SNF2 (215) ------YAMCNSFHQRIKAPIDKK------
At5g05130 SNF2 (257) ------IKSYWQSLIQRPLGQGN------
At5g22750 SNF2 (212) ------WGTWAWWNKLVQKPFEEG------
At5g43530 SNF2 (193) ------WCNWAWWSKLIQKPYENG------
At2g40770 SNF2 (163) ------RWWIEVIRDPYERR------
At3g54460 SNF2 (139) ------NPKFWEAGILRPFEAEME------
At1g48310 SNF2 (184) ------IHEYGGRYCKGGFFGTYQG------
At5g07810 SNF2 (162) ------KYEFAKTYCEVGLVRGIQGKIFQDFSKG--
At5g63950 SNF2 (192) ------WFKQNYEHYILRGTDKNATDR----E------
At1g03750 SNF2 (188) ------EHFRDFYDEPLKLGQRATAPERFVQIADK
At2g18760 SNF2 (212) ------LPVFEAEFSVPITVGG------YANASPLQ
At1g08600 SNF2 (208) ------SPEFRNRFQNPIENGQHMNSTAEDVKIMN-
At2g44980 SNF2 (185) ------LDQFLSAFKETGDGLDVSN------
At3g54280 SNF2 (185) ------ERQFQASYGKPLLAARDPKCSAKDAEAG--
At2g02090 SNF2 (196) ------VDLKKLLNAEDT------
At3g12810 SNF2 (180) ------HQEFKDWFCNPIAGMVE------GQ
At2g13370 SNF2 (185) ------KDEFVENYKNLSSFNE------
At3g06400 SNf2 (180) ------AETFDEWFQISGEN------D
At5g18620 SNF2 (180) ------AETFDEWFQISGEN------D
At4g31900 SNF2 (182) ------SLEKFQDINKEE------
At5g44800 SNF2 (192) ------LSSFEERFHDLTSAE------
At2g28290 SNF2 (182) ------SEDFSQWFNKPFQSNG-ESS-AEEALLSEE
At3g06010 SNF2 (181) ------VQNFEEWFNAPFADRG------NVSLTDE
At3g19310 SNF2 (180) ------IHNFEEWFNTPFAECG------SASLTDE
At2g46020 SNF2 (179) ------RKAFHDWFAQPFQKEGPAHN-IEDDWLETE
At3g57300 SNF2 (186) ------HDQFNEWFSKGIENHAE------HG
At5g66750 SNF2 (183) ------HDEFESWFDFSEKNKN------EATKEEE
At1g05480 SNF2 (240) ------L----TSTLKKSGMTVTKRGKKNLGNEINNR----
At3g24340 SNF2 (222) ------I----SSRIHELSKCSQE-GEHGRVNEENR-----
At2g21450 SNF2 (197) ------G----TREIVSRIMSKAEIPRGKQVNQSSSSIEGT
At2g16390 SNF2 (195) ------T----SKSAVKRILAYTPCDVRGRLTGSNSDMASM
At5g20420 SNF2 (228) ------V----LMELDQKFKTNHGVNKAPHLLENRARKLFL
At3g42670 SNF2 (196) ------V----LVELDKKFQTNQAEQKAPHLLENRARKFFL
Consensus (451) F
*
501 550
At1g02670 SNF2 (213) ------KFLIGGNILPLS------IPVRIENVP----A
At1g05120 SNF2 (318) ------SFGLGKRAMILLKHKVLKDILLRRTKLG----R
At1g11100 SNF2 (245) ------PGEGYKTLQAILKK-----VMLRRTKDTLL-DG
At1g61140 SNF2 (304) ------PVKGYQKLQAILKT-----VMLRRTKGSLL-DG
At1g50410 SNF2 (318) ------SLQGYKKLQAVLRA-----IMLRRTKGTLL-DG
At3g20010 SNF2 (334) ------SCQGYKKLQAVLRA-----IMLRRTKGTLL-DG
At3g16600 SNF2 (233) ------PLHGYKKLQAILRG-----IMLRRTK------
At5g05130 SNF2 (274) ------KKGLSRLQVLMAT-----ISLRRTKEKSLIG-
At5g22750 SNF2 (230) ------DERGLKLVQSILKP-----IMLRRTKSSTDREG
At5g43530 SNF2 (211) ------DPRGLKLIKAILRP-----LMLRRTKETRDKEG
At2g40770 SNF2 (177) ------DTKAMEFTHKFFKQ-----VMWRSSKVHVADEL
At3g54460 SNF2 (157) ------EGRLRLLQLLQR-----CMISSRKK------
At1g48310 SNF2 (203) ------ASNHDELHNLMKAT----VMIRRLKKDVLTEL
At5g07810 SNF2 (190) ------TRLLELNILLNQT----VMIRRLKQHLLTQL
At5g63950 SNF2 (214) ------QRIGSTVAKNLREHIQP-----FFLRRLKSEVFGDD
At1g03750 SNF2 (217) ------RKQHLGSLLRK-----YMLRRTKEETIG--
At2g18760 SNF2 (236) VST------AYRCAVVLRDLIMP-----YLLRRMKADVNAHL
At1g08600 SNF2 (237) ------Q--RSHILYEQLKG-----FVQRMDMN--VVKK
At2g44980 SNF2 (204) ------DKETYKSLKFILG-----AFMLRRTKSLLIESG
At3g54280 SNF2 (213) ------VLAMEALHKQVMP-----FLLRRTKEEVLSD-
At2g02090 SNF2 (208) ------ELITRMKSILGP-----FILRRLKSDVMQQL
At3g12810 SNF2 (199) EKI------NKEVIDRLHNVLRP-----FLLRRLKRDVEKQ-