Session 2009-2010
FACULTY OF AGRICULTURE
UNIVERSITY OF RAJSHAHI
RAJSHAHI
DEPARTMENT OF GENETIC ENGINEERING AND BIOTECHNOLOGY
The courses and Curricula layout for a four-Years B.Sc (Honours) in the department of Genetic
Engineering & Biotechnology is presented as below.
1. Total number of units is 40 spread over 4 years.
2. Each theory unit will be of 100 marks ¾ unit for 75 marks and ½ unit for 50 marks.
3. The duration of examinations of the theory courses shall be 3 and 4 hours for 0.50 and 0.75 unit courses respectively. The duration of practical examination shall be 6 hours for o.50 units. For other fractions of a unit, proportionality shall be applied.
Mark distributions: The Year-wise distribution of marks among the theory, practical, viva-voce, class assessment/ Field report/examination/ Tutorial/ terminal/ Class attendance, thesis/ dissertation/ project etc. shall be as follows:
Each theory consists of 80% course examination and 20% tutorial examination.
(i)B.Sc. Houours Part-1 Examination
Nature of course / Units / Marks / CreditsTheory (including related) / 6 / 600 / 24
Practical / 2 / 200 / 8
Viva-voce / 1 / 100 / 4
Class assessment /tutorial/terminal /class attendance/ Field report/ examination, etc / 0.5 / 50 / 2
Total (credit courses) / 9.5 / 950 / 38
(ii) B. Sc. Honours Part-2 Examination:
Nature of course / Units / Marks / CreditsTheory (including related) / 5.5 / 550 / 22
Practical / 2.5 / 250 / 10
Viva-voce / 1.0 / 100 / 4
Class assessment /tutorial/terminal /class attendance/ Field report/ examination, etc2 / 0.5 / 50 / 2
Total (credit courses) / 9.5 / 950 / 38
(iii) B. Sc. Honours Part-3 Examination:
Nature of course / Units / Marks / CreditsTheory (including related) / 7.25 / 725 / 30
Practical / 2.0 / 200 / 8
Viva-voce / 0.5 / 50 / 2
Class assessment /tutorial/terminal /class attendance/ Field report/ examination, etc / 0.75 / 75 / 2
Total (credit courses) / 10.5 / 1050 / 42
(iv) B. Sc. Honours Part-4 Examination:
Nature of course / Units / Marks / CreditsTheory (including related) / 6.75 / 675 / 28
Practical / 2.0 / 200 / 8
Viva-voce / 0.5 / 50 / 2
Class assessment /tutorial/terminal /class attendance/ Field report/ examination, etc / 0.75 / 75 / 2
Project/Thesis/dissertation in –plant training/special practical / 0.50 / 50 / 2
Total (credit courses) / 10.5 / 1050 / 42
130% of the total practical marks shall be allotted for continuous laboratory assessment.
220% of the assessment marks shall be awarded for attendance in the class on the basis of the following table:
3 Theoretical course(s) may be offered instead, it a department desires.
Table for awarding marks for attendance:
Attendance / Marks90% and above / 10
80% and above / 9
70% and above / 8
60% and above / 7
Less then 60% / 0
FIRST YEAR
Examination: Part-I, Year: 2009
Course No / Course Title / Marks / UnitTHEORY COURSES
Honours Courses:
GEB-101 / Introductory Biotechnology / 75 (¾Unit)
GEB -102 / Fundamentals of Genetics / 75 (¾Unit)
GEB -103 / Cell Biology / 75 (¾Unit)
GEB -104 / Microbiology / 75 (¾Unit)
GEB -105 / Molecular Biology-I / 75 (¾Unit)
RELETED Courses
GEB -106 / Fundamentals of Biochemistry / 75 (¾Unit)
GEB -107 / Fundamentals of Botany / 75 (¾Unit)
GEB -108 / Fundamentals of Zoology / 75 (¾Unit
HONOURS PRACTICAL COURSES
GEB -109 / Covering courses 101 & 105 / 50 (½Unit )
GEB -110 / Covering courses 102 & 103 / 50 (½Unit)
Class assessment: Tutorial / Class attendance / Field report or Excursion. (25+10+15) / 50 (½Unit)
Viva-voce / 50 (½Unit)
RELATED PRACTICAL COURSES
GEB -111 / Covering course 107 / 50 (½Unit)
GEB -112 / Covering course 108 / 50 (½Unit)
Viva-voce / 50 (½Unit)
950(9.5 Unit)
SECOND YEAR
Examination: Part-II, Year: 2010
Course No / Course Title / Marks / UnitTHEORY COURSES
Honours Courses|
GEB-201 / Molecular Biology-II / 75(¾Unit)
GEB -202 / Plant Developmental Biology / 75(¾Unit)
GEB -203 / Animal Developmental Biology / 75(¾Unit)
GEB -204 / Plant Breeding-I / 75(¾Unit)
GEB -205 / Physiology and Biochemistry of Plants / 50(½Unit)
GEB -206 / Human and Animals Physiology / 50(½Unit)
GEB -207 / Bioinformatics and Computer Application / 50(½Unit)
RELETED Courses
GEB -208 / Food and Nutrition Biochemistry / 50(½Unit)
GEB -209 / Plant Pathology / 50(½Unit)
PRACTICAL COURSES: Honours
GEB -210 / Covering courses 201, 203 & 206 / 50(½Unit)
GEB -211 / Covering courses 202, 204 & 205 / 50(½Unit)
GEB -212 / Covering courses 104 & 207 / 50(½Unit)
Class assessment: Tutorial / Class attendance Field report of Excursion. (25+10+15) / 50(½Unit)
Viva-voce / 50(½Unit)
RELETED COURSES
GEB -213 / Covering course 106 & 208 / 50(½Unit)
GEB -214 / Covering course 209 / 50(½Unit)
Viva-voce / 50(½Unit)
950 (9.5 Unit)
THIRD YEAR
Examination: Part-III, Year: 2011
Course No / Course Title / Marks / UnitTHEORY COURSES :
Honours Courses
GEB-301 / Recombinant DNA Technology / 75(¾Unit)
GEB -302 / Plant Cell and Tissue Culture / 75(¾Unit)
GEB -303 / Biotechnology for Crop Protection / 75(¾Unit)
GEB -304 / Animal Biotechnology / 75(¾Unit)
GEB -305 / Molecular and Microbial Genetics / 75(½Unit)
GEB -306 / Cytogenetics / 75(½Unit)
GEB -307 / Biostatistics and Experimental Design / 75(½Unit)
GEB -308 / Plant Breeding-II / 75(¾Unit)
GEB -209 / Animal Breeding / 75(¾Unit)
GEB-310 / Population Genetics / 50(½Unit)
PRACTICAL COURSES: Honours
GEB -311 / Covering Courses 301.302 & 303 / 50(½Unit)
GEB -312 / Covering Courses 304.308 & 309 / 50(½Unit)
GEB -313 / Covering Courses 305 & 306 / 50(½Unit)
GEB -314 / Covering Courses 307 & 310 / 50(½Unit)
Class assessment: Tutorial / class attendance / Field report orExcursion (40+10+25) / 75(¾Unit)
Viva-voce / 50(½Unit)
1050 (10.5 Unit)
FORTH YEAR
Examination: Part-IV, Year: 2012
Course No / Course Title / Marks/UnitTHEORY COURSES: Honours Courses
GEB-401 / Biomedical Science and Genetic Engineering / 75 (¾ Unit)
GEB-402 / Industrial, Pharmaceutical and Environmental Biotechnology / 75 (¾ Unit)
GEB-403 / Immunology and Immunogenetics / 75 (¾ Unit)
GEB-404 / Agricultural Biotechnology / 75 (¾ Unit)
GEB-405 / Biotechnology of Microbial Systems / 75(¾ Unit)
GEB-406 / DNA Fingerprinting Molecular Diagnostics / 50 (½ Unit)
GEB-407 / Enzyme and Enzyme Kinetics / 75 (¾ Unit)
GEB-408 / Cancer Biology / 50 (½Unit)
GEB-409 / Biometrical Genetics / 75 (¾ Unit)
GEB-410 / Biophysical Methods in Life Sciences / 50 (½Unit)
PRACTICAL COURSES:
GEB-411 / Covering Courses 401, 403 and 408 / 50 (½Unit)
GEB-412 / Covering Courses 402 and 404 / 50 (½Unit)
GEB-413 / Covering Courses 405 and 406 / 50 (½Unit)
GEB-414 / Covering Courses 407, 409 & 410 / 50 (½Unit)
GEB-415 / Class Assessment: Tutorial / Class attendance / and Field report of Excursion (40+10+25) / 75 (¾Unit)
GEB-416 / Project Paper / Thesis / Dissertation / 50 (½Unit)
Viva voce / 50 (½Unit)
1050 (10.5)
FIRST YEAR
Examination: Part-I, Year: 2009
Course No / Course Title / Full Marks / UnitTHEORY COURSES Honours Courses:
GEB-101 / Introductory Biotechnology / 75(¾Unit)
GEB -102 / Fundamentals of Genetics / 75(¾Unit)
GEB -103 / Cell Biology and Cytology / 75(¾Unit)
GEB -104 / Microbiology / 75(¾Unit)
GEB -105 / Basic Molecular Biology-I / 75(¾Unit)
RELETED Courses
GEB -106 / Fundamentals of Biochemistry / 75(¾Unit)
GEB -107 / Fundamentals of Botany / 75(¾Unit)
GEB -108 / Fundamentals of Zoology / 75(¾Unit)
HONOURSE PRACTICAL COURSES
GEB -109 / Covering courses 101 & 105 / 50(½Unit )
GEB -110 / Covering courses 102 & 103 / 50(½Unit)
Class assessment: Tutorial / Class attendance / Field report or Excursion. (25+10+15) / 50(½Unit)
Viva-voce / 50(½Unit)
RELETED PRACTICAL COURSES
GEB -111 / Covering course 107 / 50(½Unit)
GEB -112 / Covering course 108 / 50(½Unit)
Viva-voce / 50(½Unit)
950 (9.5 Unit)
GEB-101
Introductory Biotechnology
Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) / Time: 4 Hours1. / Introduction: Definition, old and new biotechnology and interdisciplinary activity, scope and importance. Commercial potentiality.
2. / Plant Biotechnology: Introduction, origin, history, laboratory organization, nutrition media. Sterilization type of culture. Large scale culture. Advantages and limitations of different types of culture.
3. / Animal Biotechnology: Introduction, history of organ culture, techniques, advantages, limitations and application. Introduction to cell culture, substrate, culture media, preparation of substrate and media, isolation of explants and desegregation of explants.
4. / Recombinant DNA technology: Definition, restriction endonucleases, modifications of cut ends. Steps in gene cloning, isolation of desired gene, uses of plasmid and marker genes. Gene transfer methods, Advantage and disadvantage of Agrobacterium mediated and biolistic gene transfer method.
5. / Biotechnology and health care: Introduction, disease prevention, disease diagnosis, disease treatment, gene therapy and forensic medicine.
6. / General and industrial microbiology: Historical landmarks, isolation and screening of microorganisms, inoculums development and application.
7. / Enzyme technology: Fermentation, cheese production and use of enzyme in food industry.
8. / Fuel and environment biotechnology: Introduction, types and uses of fuel. Concept of bio-fuel and biogas and their uses. Biotechnology in pollution control.
9. / Biotechnology in Agricultural Science. Biotechnology in pollution control.
Book References:
Waston, J, D., Tooze, J. and Kurtz, D.T. 1983, Recombinant DNA. A short course, scientific American Books, New york.Drlica, K. 1984, Understanding DNA and Gene Cloning A Guide for the Curious, John Wiely & Sons, New York.
Steven, P. 1984, Biotechnology-A New Industrial Revolution. George Braziller Inc. USA.
Antebi E. and Fishiock D. 1986, Biotechnology. The Mit Press, USA.
Marx J.L. 1989, A Revolution in Biotechnology Cambridge Univ. Press, UK. Old, Principles of Gene Manipulation and Introduction to Genetic Engineering. 3rd Ed.
Koshland, Biotechnology.
Smith. 1996, Biotechnology.
Rehm. 1986. Biotechnology.
Brown. 1987, Introduction to Biotechnology.
Kingsman and Kingsman.1988, Genetic Engineering.
B.D. Singh. Biotechnology. Expanding Horizons.
G. R. Chhatwal.1998, Textbook of Biotechnology.
P. K. Gupta. The Elements of Biotechnology.
P. R. Trived and K. N. Sudarshan: Environment and natural resources conservation.
M. M. Ranga. Animal Biotechnology.
GEB-102
Fundamentals of Genetics
Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) / Time: 4 Hours1. / Historical background of Genetics
2. / Mendel and his predecessors.
3 / Mendel’s Law of Segregation and Law of Independent Segregation of alleles.
4. / Probability and chi-square test.
5. / Physical basis of heredity: Evidence for the chromosomal theory of inheritance.
6. / Gene symbols: Dominance and lethal gene action.
7. / Gene Interaction: Types of gene interaction and genetic explanation.
8. / Linkage, crossing over and chromosome map.
9. / Sex determination in plants & animals.
10 / Sex linkage, sex linked inheritance, sex influenced & sex limited traits.
11. / Multiple alleles.
12. / Inheritance of quantitative characters.
13. / Hardy- Weinberg law and population.
14. / Mutation: characteristics, classification, induction and detection.
15. / Cytoplasmic inheritance.
16. / Male sterility: Types and genetic control.
17. / Pedigree analysis, twins, human traits, disorders due to mutant genes.
Book References:
Snustad, D.P. Simmons, M.J. and Jenkins J.B. 1997. Principles of Genetics, Jacaranda/Wiely publishers.Klung, W.S. and Cummings, M.R. 1980, Concepts of Genetics. Scott, Foresman and Company USA.
Waston, J.D. and Hopkins, A.M., Roberts, J.W., Steitz, J.A. and Weiner, A.M. 1988. Molecular Biology of the Gene.
Benjamin, Cummings, Scientific Publishing. Menlo Park, California.
Lewin, B. Genes, VII., Oxford University Press, seventh, Editions.
A.V.S.S Sambamurty. Genetics 2nd Edition. Narosa Publishing House, New Delhi.
B. D. Singh: Fundamentals of Genetics.
Strickberger: Fundamentals of Genetics.
Verma and Agarwal. Genetics
.
GEB-103
Cell Biology
Full Marks: Theory 60 + Tutorial 15 = 75 (3/4 Unit) / Time: 4 Hours1. / A brief introduction: Historical background of cell biology. Concept of life and living process, the identifying characteristics of a living matter. Concept, size and structure of prokaryotic and eukaryotic cell and their differences. Cell theory: Modern techniques of study of the cell fractionation.
2. / Cell Membrane: Physical and chemical structure of cell membrane. Its organization and function. Membrane models: Danielli-Davson, Robertson, Nicholson-Singer models. Lipid bi-layer and functions of cell membranes. Membrane Transport: ways to move molecules across membrane Carrier proteins: Lon channels: Cell signaling.
3. / Structural basis of the cell: Cytoskeleton, microtubules, micro-filaments and intermediate filaments.
4. / Cytoplasm and Cytoplasmic Organelles: Cytoplasmic matrix, ribosome’s vacuolar system and microsomes, mitochondria and chloroplasts, endoplasmic reticulum. golgi complex with their ultra structure and function. lysosomes. peroxisome and glyoxysomes.
5. / Protein folding and structure: Primary, secondary and tertiary structure.
6. / Physical and Chemical structure of Chromosomes: Structure of chromatin, nucleosome shape and organization. Higher order structure of chromatin, organization of chromosome. Centromere, telomere, nucleosome and its organization in eukaryotic chromosome.
7. / Cell division: Cell cycle, Mitosis, Meiosis and their biological significance.
Book References:
Alberts. et al, 2002, Molecular Biology of the cell. 4th edition.Alberts. et al, 2004, Essential Cell Biology, 2nd edition.
Verma. P.S. and Agarwal V.K. 1974, Cell Biology, Genetics, Molecular Biology and Evolution. S.Chand & Company Ltd. Ram Nagar, New Delhi- 110055
Snyder. L.H. and David. P. R. 1957, The Principles of Heredity. D. C. Health and company, Boston.
Avers C.J. 1980, Genetics D. Van Nostrand Company, New York, Cincinnati, Toronto, London Melbourne.
Strickberger M.W. 1995, Genetics. Prentice-Hall of India Private Ltd. New Delhi-110001.
Verma. P.S & Agarwal. V.K. 1975, Genetics. S. Chard and Company Ltd, Ram Nagar, New Delhi- 110055
Power. C.B. 1997, Cell Biology. Himalaya Publishing House, Bombay-400004.
Molecular Cell Biology. H.S. Bhamrah.
Sowanson. Cytogenetics.
M.S. Sominath. Cell Biology.
Bruce Alberts., D. Bray. J. Lewis, M. Raff. K. Roberts, J. D. Watson. Molecular Biology of the Cell.
Lehninger. A. Principles of Biochemistry, Arora, M. Molecular Biology.
De Robertis, E D. P. and De Robertis, E.M.F. Cell and Molecular Biology.
Hall J.L.Flowers, T. J. and Roberts.R.M. Plant Cell Structure and Metabolism
Verma. P. S. and Agarwal V.K. Cytology.
Verma. P. S. Cell Biology.
Gerald Karp. Cell and Molecular Biology Concepts and Experiments.
S. C. Rastogi. Cell Biology. New Delhi, Tata Mc Graw hill pub, Com, Ltd.
David E. Sadova. Cell Biology Organelle Structure & Function. Boston, Jones & Bartlet Publication.
GEB-104
Microbiology