Supplement Table 1.Positive Studies of HPV DNA in Breast Cancer Tissue Using PCR

Supplement Table 1.Positive studies of HPV DNA in breast cancer tissue using PCR

Time / Author / Country / Number
(specimens) / Number of
positivespecimens / HPV types / Primer type
1992 / Di Lonardo et al.37 / Italy / 40 breast carcinomas
40 lymph nodes metastases / 5
2 / HPV-16
HPV-16 / HPV-11/16/18
E6
1999 / Hennig et al.38 / Norway / 41 breast carcinomas
from 38 CIN III patients / 19 / HPV-16 / HPV-11/16/18/33
L1 ( GP5+/GP6+)
E6/E7
2000 / Yu et al. 40 / China / 32 invasive ductal breast carcinomas / 14 / HPV-33 (14)
HPV-16 (0)
HPV-18 (0) / HPV-16/18/33
2001 / Liu et al.41 / China / 17 breast carcinomas / 6 / HPV-11/16/18
L1 region E6/E7
2004 / Damin et al.42 / Brazil / 41 benign breast diseases
101 breast carcinomas / 0
25 / HPV-16 (14)
HPV-18 (10)
HPV-16-18（1） / HPV-11/16/18
2004 / Widschwendter et al.43 / Australia / 11 breast carcinomas / 7 / HPV-16 (6)
Other types (1) / HPV-16/18/33
L1 (GP5+/GP6+)
2005 / de Villiers et al.44 / USA / 29 breast carcinomas
(29 nipple tissue samples from these breasts) / 25
(20) / HPV-16 most
HPV- 6 less
HPV-18 none / HPV-6/11/18
L1 ( CP,FAP, GP5+/GP6+)
2005 / Kan et al. 45 / Australia / 50 unselected invasive
breast carcinomas / 24 / HPV-18 (24)
HPV-16 (0)
HPV-33 (0) / HPV-16/18/33
E6
2006 / Kroupis et al.46 / Greece / 107 breast cancers / 17 / HPV-16 (14)
HPV- 59 (3)
HPV- 58 (2)
HPV- 73 (1)
HPV- 82 (1) / 21 types of high risk HPV
L1 (MY, GP5+/GP6+)
E4/E6/E7
2006 / Gumus et al.47 / Turkey / 50 breast cancers
50 normal breast tissues / 37
16 / HPV-18 (20)
HPV-33 (35)
HPV-18 (9)
HPV-33 (14) / HPV-11/16/18/33
L1 (MY09/11)
2007 / Yasmeen et al.49 / Canada / 27 breast cancers
18 normal breast tissues / 23
2 / HPV-16
HPV-16 / HPV-16/18/31/33/35
L1 (MY09/11)
E6/E7
2007 / Choi et al.61* / Korea / 123 breast carcinomas
31 intraductal papillomas / 8
0 / high-risk HPV / High-risk HPV
2008 / Kulka et al.50 / Hungary / 20 Lymphoepithelioma-like carcinomas
(EBV-) / 20 / HPV-18 (+)
HPV-33 (+) / HPV-16/18/33
E6
2008 / Sarkola et al.51 / Turkey / 223 breast milk samples obtained 3 days postpartum / 10 / HPV-16 / HPV-16/33
E6
2008 / Akil et al.52 / Syrian / 113 breast cancers
(87 invasive)
(26 in situ) / 69 / HPV-16 (10)
HPV-18 (11)
HPV-31 (8)
HPV-33 (63)
HPV-35 (42) / HPV-16/18/31/33/35
E6/E7
2009 / de León et al.36 / Mexico / 51 breast carcinomas
43 non-malignant tissues / 15
0 / HPV-16 (10+2)
HPV-18 (3+2) / HPV-16/18
L1 (MY09/11, GP5+/GP6+)
2008 / Khan et al.59* / Japan / 124 breast cancers / 26 / HPV-16 (92%)
HPV-6 (46%)
HPV-18 (12%)
HPV-33 (4%) / HPV-16/18/33
L1 (SPF10)
E6
2009 / Heng et al.53 / Australia / 13 invasive ductal carcinomas
13 ductal carcinoma in situ samples
17 normal breast tissue samples / 5
3
3 / HPV-18 (+) / HPV-16/18
L1
2009 / Mendizabal-Ruiz et al.60* / Mexico / 67 breast cancers
40 non-malignant breast diseases / 3
0 / HPV-16/18/33

HPV, human papillomavirus; PCR, polymerase chain reaction

*These three studies could not demonstrate whether or not such HPVs directly contribute to the development of breast cancer, even HPV was detected.