Supplemental table 1. Surveillance of aquatic animal viruses from cultured salmonids in Korea between February 2013 and April 2015
Sampling sites(Farms) / Month/year / Species / Environmentalcondition / No. of fish / Size / Clinical sign / Virus
(Cultured on EPC cell line)
Temperature
(℃) / DO
(mg/L) / pH / Salinity
(‰) / (Means±SD cm)
Chuncheon
CcA / Mar-14 / rainbow trout / 13.7 / 7.7 / 8.0 / 0.1 / 20 / 5.6±0.4 / - / -
Oct-14 / rainbow trout / 16.9 / 9.3 / 8.6 / 0.1 / 8 / 22.7±2.0 / gill hyperemia / -
Mar-15 / rainbow trout / 10.8 / 11.8 / 8.1 / 0.1 / 30 / 8.3±0.7 / - / -
CcB / Mar-14 / rainbow trout / 10.6 / 9.2 / 7.8 / 0.1 / 20 / 10.2±1.1 / gill anemia, mucus hypersecretion / -
Oct-14 / rainbow trout / 16.0 / 8.9 / 8.1 / 0.1 / 12 / 24.1±5.1 / gill congestion, mucus hypersecretion
Mar-15 / rainbow trout / 7.1 / 12.4 / 8.0 / 0.2 / 40 / 12.9±1.4 / -
CcC / Mar-15 / rainbow trout / 15.0 / 11.2 / 8.9 / 0.1 / 30 / 10.6±0.8 / -
Gangneung
GnA / Mar-14 / rainbow trout / 14.6 / 9.2 / 8.4 / 0.2 / 10 / 31.4±3.6 / - / -
Oct-14 / rainbow trout / 13.5 / 10.0 / 8.3 / 0.1 / 8 / 27.5±7.2 / gill hyperemia, mucus hypersecretion / -
GnB / Mar-14 / rainbow trout / 10.8 / 10.7 / 8.5 / 0.1 / 8 / 19.7±6.9 / gill congestion, mucus hypersecretion / IHNV
(+ve)
Hwacheon
HcA / Jun-13 / masu salmon / NTa / NT / NT / NT / 6 / 10.6±1.5 / gill anemia / IHNV
(-ve)
Jun-14 / masu salmon / NT / NT / NT / NT / 14 / 12.5±1.5 / - / -
Apr-15 / masu salmon / 10.9 / 17.1 / 8.1 / 0.1 / 44 / 8.5±1.1 / - / -
HcB / Apr-15 / masu salmon / 11.3 / 20.2 / 8.3 / 0.1 / 38 / 9.6±0.7 / mucus hypersecretion
Jeongseon
JS / Feb-15 / rainbow trout / 12.8 / 10.8 / 8.4 / 0.3 / 20 / 11.3±1.1 / - / -
Pyeongchang
PcA / Mar-13 / rainbow trout / 11.3 / 10.9 / 8.6 / 0.2 / 12 / 13.2±1.4 / gill hyperemia
Apr-13 / rainbow trout / NT / NT / NT / NT / 20 / 13.9±2.6 / gill hemorrhage / IHNV
(-ve)
Aug-13 / rainbow trout / 14.0 / 11.0 / 8.8 / 0.7 / 10 / 20.1±0.9 / mucus hypersecretion
Nov-13 / rainbow trout / 11.5 / 14.3 / 8.1 / 0.2 / 11 / 16.1±2.1 / gill hemorrhage
Feb-14 / rainbow trout / 12.2 / 10.3 / 8.8 / 0.2 / 20 / 7.8±5.8 / gill anemia, necrosis of pectoral fin
Sep-14 / rainbow trout / 13.8 / 12.5 / 8.2 / 0.2 / 30 / 8.5±5.5 / gill hyperemia, mucus hypersecretion
Feb-15 / rainbow trout / 9.4 / 9.4 / 8.4 / 0.2 / 20 / 20.1±1.7 / mucus hypersecretion / -
PcB / Mar-13 / rainbow trout / 11.2 / 12.0 / 8.5 / 0.1 / 11 / 22.7±2.3 / mucus hypersecretion
May-13 / rainbow trout / 12.5 / 12.9 / 8.8 / 0.1 / 13 / 17.3±2.1 / gill hyperemia
Aug-13 / rainbow trout / 13.0 / 9.1 / 8.8 / 0.2 / 4 / 23.2±3.2 / gill hyperemia
Nov-13 / rainbow trout / 10.9 / 7.6 / 8.1 / 0.1 / 7 / 12.5±1.4 / gill hyperemia
PcC / Mar-13 / rainbow trout / 9.0 / 13.0 / 8.2 / 0.1 / 8 / 22.3±1.1 / - / -
Mar-13 / rainbow trout / 8.8 / 12.8 / 8.3 / 0.1 / 20 / 7.4±0.8 / - / -
May-13 / rainbow trout / 11.5 / 12.8 / 8.8 / 0.1 / 10 / 10.5±1.3 / mucus hypersecretion
Aug-13 / rainbow trout / 15.8 / 10.4 / 8.3 / 0.1 / 20 / 8.5±1.2 / gill anemia
Nov-13 / rainbow trout / 13.2 / 14.7 / 8.1 / 0.1 / 15 / 10.6±0.9 / gill anemia
Mar-14 / rainbow trout / 9.9 / 11.5 / 8.2 / 0.1 / 4 / 19.1±0.8 / mucus hypersecretion / IHNV
(+ve)
Oct-14 / rainbow trout / 14.5 / 9.1 / 8.3 / 0.1 / 7 / 29.4±2.1 / gill hyperemia
Feb-15 / rainbow trout / 10.7 / 13.9 / 8.6 / 0.1 / 20 / 10.2±0.8 / -
PcD / Mar-14 / rainbow trout / 14.5 / 9.1 / 8.3 / 0.1 / 9 / 19.4±1.3 / - / -
Oct-14 / rainbow trout / 14.6 / 9.2 / 8.4 / 0.2 / 9 / 19.2±2.0 / gill anemia, mucus hypersecretion
Mar-15 / rainbow trout / 14.5 / 10.8 / 8.2 / 0.2 / 29 / 7.3±1.0 / - / IHNV
(+ve)
PcE / Mar-14 / rainbow trout / 10.1 / 11.3 / 8.3 / 0.2 / 3 / 15.3±0.4 / - / IHNV
(+ve)
Mar-15 / rainbow trout / 9.6 / 12.1 / 8.0 / 0.2 / 30 / 8.3±0.9 / - / -
PcF / Feb-14 / rainbow trout / 12.4 / 10.1 / 8.1 / 0.2 / 20 / 6.2±0.5 / mucus hypersecretion
Oct-14 / rainbow trout / 14.0 / 9.7 / 8.2 / 0.2 / 12 / 18.5±2.9 / gill hyperemia, anemia
Feb-15 / rainbow trout / 9.8 / 10.2 / 8.3 / 0.2 / 20 / 13.3±2.5 / gill congestion, mucus hypersecretion
PcG / Mar-14 / rainbow trout / NT / NT / NT / NT / 20 / 13.0±1.6 / - / IHNV
(+ve)
Jun-14 / rainbow trout / NT / NT / NT / NT / 13 / 14.7±6.2 / gill anemia / IHNV
(+ve)
PcH / Feb-14 / rainbow trout / 12.2 / 8.7 / 8.2 / 0.2 / 20 / 3.4±0.3 / exophthalmos (pop-eye), gill anemia
Oct-14 / rainbow trout / 13.7 / 7.3 / 8.3 / 0.2 / 11 / 21.8±3.8 / gill hyperemia
Feb-15 / rainbow trout / 7.8 / 12.0 / 8.8 / 0.2 / 40 / 9.0±0.8 / - / IHNV
(-ve)
PcI / Mar-14 / rainbow trout / 10.6 / 9.2 / 7.8 / 0.1 / 20 / 7.8±0.8 / mucus hypersecretion
Oct-14 / rainbow trout / 12.4 / 11.6 / 8.5 / 0.2 / 8 / 18.4±5.1 / gill congestion / -
Yangyang
YyA / Feb-13 / masu salmon / 7.3 / 11.8 / 7.8 / 0.2 / 34 / 3.2±0.2 / mucus hypersecretion / IHNV
(+ve)
May-13 / masu salmon / 11.1 / 12.4 / 8.3 / 0.1 / 20 / 4.3±0.5 / gill hyperemia, mucus hypersecretion
Aug-13 / masu salmon / 20.5 / 10.8 / 8.0 / 0.2 / 20 / 8.0±0.7 / -
Nov-13 / masu salmon / 15.2 / 11.0 / 8.2 / 0.4 / 13 / 11.5±1.7 / gill hyperemia, mucus hypersecretion
Feb-14 / masu salmon / 6.2 / 9.3 / 8.3 / 0.5 / 28 / 3.1±2.6 / - / -
Apr-14 / masu salmon / NT / NT / NT / NT / 25 / 15.1±1.2 / gill hyperemia, mucus hypersecretion / IHNV
(+ve)
Sep-14 / masu salmon / 17.5 / 9.5 / 9.1 / 0.2 / 15 / 14.7±2.1 / gill congestion / -
Jan-15 / masu salmon / 7.3 / 12.2 / 8.1 / 0.2 / 40 / 2.5±0.2 / - / -
Feb-13 / rainbow trout / 7.3 / 12.2 / 7.7 / 0.2 / 10 / 16.2±0.7 / necrosis of pectoral fin
May-13 / rainbow trout / 11.0 / 13.2 / 7.9 / 0.1 / 20 / 4.1±0.3 / mucus hypersecretion
Aug-13 / rainbow trout / 20.5 / 10.4 / 7.8 / 0.2 / 20 / 7.8±0.8 / - / -
Nov-13 / rainbow trout / 15.1 / 10.3 / 8.0 / 0.4 / 13 / 11.3±1.4 / gill anemia / IHNV
(+ve)
Feb-14 / rainbow trout / 6.3 / 9.2 / 8.3 / 0.5 / 10 / 14.2±0.9 / gill congestion / -
Sep-14 / rainbow trout / 17.5 / 8.9 / 8.8 / 0.2 / 15 / 12.1±1.0 / - / -
Jan-15 / rainbow trout / 7.3 / 12.1 / 8.0 / 0.2 / 40 / 3.0±0.3 / - / -
YyB / Jun-13 / masu salmon / NT / NT / NT / NT / 15 / 10.9±2.6 / gill anemia / IHNV
(+ve)
Jun-13 / rainbow trout / 12.5 / 12.3 / 8.6 / 0.0 / 5 / 14.3±0.5 / exophthalmos (pop-eye), gill anemia / IHNV
(+ve)
Jan-15 / rainbow trout / 15.8 / 9.9 / 8.9 / 0.1 / 40 / 9.0±0.8 / mucus hypersecretion
Yeongwol
YwA / Mar-13 / rainbow trout / 12.1 / 10.2 / 8.6 / 0.2 / 16 / 12.7±0.9 / gill hyperemia / -
May-13 / rainbow trout / 12.4 / 11.0 / 8.1 / 0.1 / 10 / 19.1±2.4 / gill hyperemia
Aug-13 / rainbow trout / NT / NT / NT / NT / 4 / 27.4±4.3 / gill anemia
May-14 / rainbow trout / NT / NT / NT / NT / 10 / 9.1±2.3 / gill anemia, mucus hypersecretion / IHNV
(+ve)
Feb-15 / rainbow trout / 9.3 / 12.4 / 8.5 / 0.2 / 20 / 10.6±0.7 / - / IHNV
(+ve)
YwB / Mar-13 / rainbow trout / 7.2 / 9.9 / 8.2 / 0.1 / 5 / 24.7±4.5 / - / -
May-13 / rainbow trout / 11.1 / 10.3 / 8.3 / 0.1 / 25 / 2.4±0.2 / gill anemia / -
Aug-13 / rainbow trout / 16.6 / 9.4 / 8.6 / 0.1 / 12 / 6.6±0.5 / gill anemia
Oct-13 / rainbow trout / 14.9 / 9.5 / 8.9 / 0.1 / 3 / 33.7±4.2 / - / IHNV
(-ve)
YwC / Jun-14 / rainbow trout / NT / NT / NT / NT / 6 / 13.4±1.9 / gill hyperemia
Feb-15 / rainbow trout / 10.7 / 11.7 / 8.5 / 0.1 / 28 / 5.0±0.3 / gill anemia / IHNV
(-ve)
Gumi
GuA / Mar-14 / rainbow trout / 15.7 / 9.0 / 8.5 / 0.2 / 6 / 30.8±2.9 / gill (lamella) hemorrhage
Oct-14 / rainbow trout / 15.9 / 9.1 / 8.4 / 0.2 / 12 / 27.6±4.5 / gill congestion / -
GuB / Mar-14 / rainbow trout / 15.5 / 10.7 / 8.8 / 0.2 / 17 / 6.9±1.0 / gill hemorrhage, congestion / -
Oct-14 / rainbow trout / 14.7 / 10.8 / 8.1 / 0.2 / 6 / 34.8±3.1 / - / -
Supplemental table 2. Primers used to detect aquatic animal virusesfrom salmonids.
Pathogens / Region / Primer / Sequence (5' to 3') / Reference / Manual of Diagnostic Tests for Aquatic Animals, OIE[18]
Epizootic hematopoietic necrosis virus(EHNV) / Major capsid protein (MCP) / M151 / 5'-AACCCGGCTTTCGGGCAGCA-3' / [13] / Chapter 2.3.1
M152 / 5'-CGGGGCGGGGTTGATGAGAT-3'
M153 / 5'-ATGACCGTCGCCCTCATCAC-3'
M154 / 5'-CCATCGAGCCGTTCATGATG-3'
Infectious hematopoietic necrosis virus(IHNV) / Glycoprotein (G) / Forward / 5’-AGAGATCCCTACACCAGAGAC-3’ / [4] / Chapter 2.3.4
Reverse / 5’-GGTGGTGTTGTTTCCGTGCAA-3'
Infectious salmon anemia virus(ISAV) / Genomic segment 8 / ILA1 / 5'-GGCTATCTACCATGAACGAATC-3' / [14] / Chapter 2.3.5
ILA2 / 5'-GCCAAGTGTAAGTAGCACTCC-3'
FA-3 / 5'-GAAGAGTCAGGATGCCAAGACG-3' / [3]
RA-3 / 5'-GAAGTCGATGAACTGCAGCGA-3'
Viral hemorrhagic septicemia virus(VHSV) / Nucleoprotein (N) / VN forward / 5'-ATGGAAGGAGGAATTCGTGAAGCG-3' / [22] / Chapter 2.3.9
VN reverse / 5'-GCGGTGAAGTGCTGCAGTTCCC-3'