Supplementary Material

Supplementary Table S1 Expression values of tested genes in 26 tissue samples (calculated from average Cq values) used as input in geNorm

cDNA Description /

GH720838

/

GH720843

/

GH720808

/

PP2A

/ helicase / GAPDH / α-tubulin / β-tubulin / actin / EF-1α / 18S rRNA
1st Repetition / 1_Athos roots_NaCl treated_15day-old / 0.2297 / 0.5200 / 0.1994 / 0.1432 / 0.2723 / 0.0740 / 0.5467 / 0.5548 / 0.3391 / 0.5487 / 0.0092
2_Athos roots_NaCl non-treated_15day-old / 0.3329 / 0.8930 / 0.4272 / 0.4567 / 0.3067 / 0.0896 / 0.4552 / 0.8630 / 0.6018 / 0.4270 / 0.0009
3_Athos leaves_2,4-D treated_15day-old / 1.0000 / 0.0866 / 0.3071 / 0.4290 / 0.0557 / 0.0082 / 0.5580 / 0.6698 / 0.7148 / 0.1055 / 0.0928
4_Athos roots_2,4-D treated_15day-old / 0.3373 / 0.3773 / 0.1607 / 0.4426 / 0.2037 / 0.0879 / 0.0770 / 1.0000 / 0.4841 / 0.1557 / 0.0084
5_Athos leaves_2,4-D non-treated__15day-old / 0.5903 / 0.0226 / 0.4592 / 0.7980 / 0.0507 / 1.0000 / 0.2129 / 0.7563 / 0.1369 / 0.1260 / 0.1149
6_Messire roots_Orobanche infected_15dpi / 0.2312 / 0.2354 / 0.7515 / 0.2697 / 1.0000 / 0.0284 / 0.3423 / 0.7048 / 0.7191 / 0.3481 / 0.7168
7_Messire roots_Orobanche non-infected_15dp / 0.2070 / 0.4702 / 0.2739 / 0.2714 / 0.2105 / 0.0396 / 0.6177 / 0.5651 / 0.6644 / 0.0794 / 0.2803
8_Ps624 roots_Orobanche infected_15dpi / 0.4433 / 1.0000 / 0.2020 / 1.0000 / 0.2987 / 0.1012 / 0.2188 / 0.8421 / 0.7144 / 0.4187 / 0.0365
9_Ps624 roots_Orobanche non-infected_15dpi / 0.2389 / 0.6857 / 0.2222 / 0.5410 / 0.1407 / 0.0836 / 0.2557 / 0.6272 / 0.5344 / 0.0374 / 0.0041
10_Messire roots_Orobanche infected_21dpi / 0.3995 / 0.1877 / 0.1141 / 0.4210 / 0.3714 / 0.0639 / 0.8613 / 0.7633 / 0.3324 / 1.0000 / 0.2373
11_Messire roots_Orobanche non-infected_21dpi / 0.3943 / 0.8075 / 0.3442 / 0.4567 / 0.3594 / 0.0920 / 1.0000 / 0.9009 / 0.5861 / 0.6428 / 0.2389
12_Ps624 roots_Orobanche infected_21dpi / 0.3140 / 0.5971 / 0.2208 / 0.5113 / 0.1454 / 0.1340 / 0.0719 / 0.7587 / 0.4051 / 0.1652 / 0.0375
13_Ps624 roots_Orobanche non-infected_21dpi / 0.2327 / 0.4672 / 0.3464 / 0.3954 / 0.1117 / 0.0993 / 0.1884 / 0.6045 / 1.0000 / 0.1987 / 0.0047
2nd Repetition / 14_Athos roots_NaCl treated_15day-old / 0.2043 / 0.4702 / 0.2180 / 0.2783 / 0.3298 / 0.0825 / 0.3051 / 0.5935 / 0.3920 / 0.7383 / 0.0420
15_Athos roots_NaCl non-treated_15day-old / 0.3020 / 0.8763 / 0.4218 / 0.3761 / 0.2909 / 0.1582 / 0.3590 / 0.8899 / 0.7887 / 0.3694 / 0.0041
16_Athos leaves_2,4-D treated_15day-old / 0.9493 / 0.0686 / 0.3509 / 0.3979 / 0.1674 / 0.0052 / 0.4938 / 0.8192 / 0.6876 / 0.1477 / 0.7662
17_Athos roots_2,4-D treated_15day-old / 0.3039 / 0.3821 / 0.1757 / 0.3905 / 0.2499 / 0.0799 / 0.0488 / 0.9939 / 0.6428 / 0.1652 / 0.0090
18_Athos leaves_2,4-D non-treated__15day-old / 0.4856 / 0.0261 / 0.4595 / 0.9631 / 0.0576 / 0.7797 / 0.1196 / 0.7276 / 0.1220 / 0.1083 / 0.2487
19_Messire roots_Orobanche infected_15dpi / 0.0707 / 0.1442 / 1.0000 / 0.1345 / 0.9423 / 0.0148 / 0.1859 / 0.5060 / 0.1261 / 0.2621 / 1.0000
20_Messire roots_Orobanche non-infected_15dpi / 0.0930 / 0.0900 / 0.2475 / 0.0846 / 0.1945 / 0.0216 / 0.2086 / 0.2422 / 0.2894 / 0.0478 / 0.2470
21_Ps624 roots_Orobanche infected_15dpi / 0.2793 / 0.5039 / 0.2180 / 0.2207 / 0.2078 / 0.0986 / 0.1298 / 0.5756 / 0.5861 / 0.1236 / 0.0217
22_Ps624 roots_Orobanche non-infected_15dpi / 0.1670 / 0.4471 / 0.2098 / 0.3554 / 0.1225 / 0.0717 / 0.1580 / 0.4998 / 0.6179 / 0.0918 / 0.0056
23_Messire roots_Orobanche infected_21dpi / 0.3373 / 0.1469 / 0.1263 / 0.2614 / 0.3690 / 0.0563 / 0.5971 / 0.6835 / 0.3051 / 0.8369 / 0.3311
24_Messire roots_Orobanche non-infected_21dpi / 0.3995 / 0.7441 / 0.3884 / 0.3599 / 0.3342 / 0.0836 / 0.8103 / 0.8067 / 0.7097 / 0.8369 / 0.1806
25_Ps624 roots_Orobanche infected_21dpi / 0.2775 / 0.5400 / 0.2250 / 0.5617 / 0.1617 / 0.1708 / 0.0599 / 0.9347 / 0.5415 / 0.2674 / 0.0247
26_Ps624 roots_Orobanche non-infected_21dpi / 0.1915 / 0.3749 / 0.3786 / 0.3039 / 0.1154 / 0.0528 / 0.1110 / 0.4420 / 0.5137 / 0.1382 / 0.0163

Supplementary Table S2 Composition of the different experimental subsets

cDNA Description / Abiotic stress / Biotic stress / Roots / Leaves / Roots and Leaves
(non-stressed)
1_Athos roots_NaCl treated_15day-old / x / x
2_Athos roots_NaCl non-treated_15day-old / x / x / x
3_Athos leaves_2,4-D treated_15day-old / x / x
4_Athos roots_2,4-D treated_15day-old / x / x
5_Athos leaves_2,4-D non-treated__15day-old / x / x / x
6_Messire roots_Orobanche infected_15dpi / x / x
7_Messire roots_Orobanche non-infected_15dp / x / x / x
8_Ps624 roots_Orobanche infected_15dpi / x / x
9_Ps624 roots_Orobanche non-infected_15dpi / x / x / x
10_Messire roots_Orobanche infected_21dpi / x / x
11_Messire roots_Orobanche non-infected_21dpi / x / x / x
12_Ps624 roots_Orobanche infected_21dpi / x / x
13_Ps624 roots_Orobanche non-infected_21dpi / x / x / x
14_Athos roots_NaCl treated_15day-old / x / x
15_Athos roots_NaCl non-treated_15day-old / x / x / x
16_Athos leaves_2,4-D treated_15day-old / x / x
17_Athos roots_2,4-D treated_15day-old / x / x
18_Athos leaves_2.4-D non-treated__15day-old / x / x / x
19_Messire roots_Orobanche infected_15dpi / x / x
20_Messire roots_Orobanche non-infected_15dpi / x / x / x
21_Ps624 roots_Orobanche infected_15dpi / x / x
22_Ps624 roots_Orobanche non-infected_15dpi / x / x / x
23_Messire roots_Orobanche infected_21dpi / x / x
24_Messire roots_Orobanche non-infected_21dpi / x / x / x
25_Ps624 roots_Orobanche infected_21dpi / x / x
26_Ps624 roots_Orobanche non-infected_21dpi / x / x / x

Supplementary Fig. S1 Specificity of qPCR amplification. Dissociation curves for 6 representative genes showing single peaks.

Supplementary Fig. S2 a-e Mean expression stability (M) following stepwise exclusion of the least stable gene in different experimental subsets. Recommended references for reliable normalization are shown in black bars. f Pairwise variation (V) showing the effect of adding additional reference gene on the normalisation factor for the different experimental panels. The optimal number of references is indicated with arrows.