1) Fully Automated Hematoxylin- Eosin (H&E) Staining Machine N=6 s1

Riga,

26.05.2016.

Specifications.

1) Fully automated hematoxylin- eosin (H&E) staining machine n=6

Characteristics / Specifications
General characteristics / Fully automated technique— from slide drying, staining through glass cover slipping
Integrated drying, staining, cover slipping and curing of H&E specimens
Individual slide staining technology with fresh reagent on every slide
Xylene and alcohol free processing
Ready to use reagents
Automated system reports. Barcode tracking and software integration possibility.
Throughput / At least 150 slides per hour
Consumables / Nine reagents plus coverslips
Full chain of custody / Track H&E specimens from drying to glass coverslipping
Slides / 25mm x 75mm (ISO certified), 26mm x 75mm (ISO certified
Distilled water supply hook up / Not necessary
Voltage / 230 +/- 10%
Current / 30 Amps
Frequency / 50/60 Hz
Max Width / 200 cm
Max depth / 120 cm
Max height / 250 cm

2) Automated histochemical slide staining machine n=6

Characteristics / Specifications
Fully automated / Baking, deparaffinization and staining of Special Stains
Slide carousel / At least 20 slides
Reagent carousel / At least 25 reagent positions
The special histochemical stains to be available for automated staining
AFB III Staining Kit
Alcian Blue for PAS
Alcian Blue Staining Kit
Alcian Yellow Staining Kit
Congo Red Staining Kit
Diastase Kit
Elastic Staining Kit
Giemsa Staining Kit
GMS II Staining Kit
Gram Staining Kit
Green for Trichrome
Iron Staining Kit
Jones H&E Staining Kit
Jones Light Green Staining Kit
Light Green for PAS
Mucicarmine Staining Kit
PAS Staining Kit
Reticulum II Staining Kit
Steiner II Staining Kit
Steiner Staining Kit
Trichrome II Blue Staining Kit
Trichrome III Blue Staining Kit
Trichrome III Green Staining Kit
Trichrome Staining Kit
Slides / 25 mm x 75 mm, or 26 mm x 76 mm
Voltage / 220 VAC ± 10%
Current / 4 Amps
Frequency / 50/60 Hz
Max width / 180 cm
Max depth / 120 cm
Max height / 250 cm

3) Automated IHC/ISH slide staining system n=6

Characteristics / Specifications /
Slide tray, reagent carousel / At least 30 slide positions
Maximum stained slides per day / At least 60 slides per day
Continuous and random individual slides loading
Individual slides processing
Individual temperature control
Capability / Simultaneous IHC, ISH, SISH (silver in situ hybridization), Dual Stain, and FITC (Fluorescein isothiocyanate) slide processing
Slides / 25 x 75 mm, 1 x 3 in or 26 x 76 mm positively charged slide
Max Width / 120.0cm
Max Depth / 90.0 cm
Max Height / 180.0 cm
Voltage / 230VAC
Current / 6/4 Amps
Frequency / 50/60Hz

4) Workflow solution which is interfaced with LIS (laboratory information system)

n=3

Characteristics / Specifications /
Fully automated workflow solution hardware, software and consulting connectable to the laboratory information system (LIS)
Workflow solution which is interfarble with LIS / The workflow solution is intended to improve productivity and monitor patient samples in anatomic pathology
The average workflow up to 100.000 clinical cases
The networked product should provide an interface between laboratory information system (LIS) and different staining technique (hematoxylin-eosin. histochemistry, immunohistochemistry, in situ hibridization, molecular testing)
Precise slide identification with 2 D bar codes and text
Precise patient identification
Maintenance of positive sample ID with barcode scanners at each workstation and pathologist office.
Immediately locate any patient's slide, at any time, from any last scanned location
Specimens tracking and reception, grossing and embedding, cutting, staining and pathologists office

1) Digital scanner with software n=12

Characteristics / Specifications /
Scanning / Brightfield scanning — scan a 15 x 15 mm tissue area at 20X in less than two minutes
Auto scan: Automated barcode reading, tissue identification, scanning and image compression and generation
Manual scan: User-configurable scan area for slides in diagonal full-color display LCD
Capacity / Automatic slide detection and autoloader for walk-away scanning of up to 150 slides
Automatic tissue identification / Automatic tissue region identification with option to manually override
Automatic slide detection and automatic tissue detection of Area(s) of Interest (AOIs) on slides
Slide format / 25–26 mm x 75–76 mm microscope slides
Scan magnifications / 4X, 10X, 20X and 40X
Barcode / Barcode capabilities: 1D and 2D
Light source / LED
Software / Fully integrated with digital software
Images could be sent automatically to the image server after scanning
The slide identifier could be sent to the software and could be associate the slide images with the correct case information from the laboratory information system
Integrated with software for comprehensive image viewing and annotation capabilities —
including measurement tools, free-hand mark-up and text fields
Images can be viewed at magnifications up to 80X (achieved with digital zoom)
Scan viewing / 24-bit true color. Customizable to BIF, TIFF and JPEG 2000

1) Slide labeling system n=12

Characteristics / Specifications /
General characteristics / Print up to five lines of customer-defined text and a barcode on each label
Printing possibilities / Barcodes or plain text labels for H&E and other applications as part of laboratory workflow solution
Labeling / Labels are heat and chemical resistant. Labels have a two-year shelf life
Current / 2.2 Amps
Frequency / 50/60Gz
Maximal Size (W x D x H) / 20.0 cm x 35.0 cm x 20.0 cm
Maximal weight / 3.0 kg

2) Next-gene sequencing machine n=1

Characteristics / Specifications /
Read Length / 5kb
Throughput / 3500-5000 reads per run
Reads per Run / ~100,000 shotgun, 70,000 amplicon
Accuracy / 99.99 base quality
Run Time / 10 h
Aplicons / cDNA, DNA samples
Computing / Attendant PC for run processing and data analysis included

3) Real-time polymerase chain reaction (PCR) technology for amplification and detection n=2

Characteristics / Specifications /
General characteristics / Real-time polymerase chain reaction (PCR) technology for amplification and detection
Real-time PCR with digital data capture for providing accurate qualitative results with no gray zone
Eliminates the need for equivocal sample retesting
Advanced results algorithm eliminates the need for manual curve analysis
Defined workflow User defined workflow
Barcode scanner / Max 20 characters, excluding start, stop and check characters.
Software / operating system, USB interface, software
Max Dimensions, W/D/H, cm / 80/60/60 cm
Voltage / 200-240VAC
Line frequency / 50/60Hz
Running conditions / 15-32ºC

4) Digital microscope with fluorescence filter n=2

Optical System: / CFI60 Infinity Optical System
Coarse/Fine Focusing: / Coaxial coarse/fine focusing, Focusing stroke: 30mm, Coarse: 9.33mm/rotation, Fine: 0.1mm/rotation, Minimum reading: 1um
Coarse motion torque adjustable, Refocusing function
Illumination: / High luminescent White LED Illuminator (Eco-Illumination), AC adapter (100-240V)
Eyepiece Tube: / Ergonomic Binocular Tube (for F.O.V 22mm, inclination:10-30°, extension 40mm (when optional DSC port is attached, observation/photo: 100/0, 50/50)
Eyepiece Lens: / 10×(F.O.V.: 22mm),
Nosepiece: / Sextuple nosepiece
Stage / Ceramic-coated Stage without specimen holder (for 1slide (opens to left) can be attached)
Cross travel 54 (Y) × 78 (X) mm, with vernier calibrations, stage handle height and torque adjustable
Observation Method: / Brightfield, Epi-fluorescence
Objectives / CFI Plan Fluor 4X N.A. 0.13, W.D. 17.1 mm
CFI Plan Fluor 10X N.A. 0.30, W.D. 16.0 mm
CFI Plan Fluor 20X N.A. 0.50, W.D. 2.1 mm Spring-loaded
CFI Plan Fluor 40X N.A. 0.75, W.D. 0.66 mm +Spring-loaded
CFI Plan Fluor 60XA N.A. 0.85, W.D. 0.3 mm
Spring-loaded, Cover glass correction: 0.11-0.23 mm
Condenser / Condenser focusing stroke: 27mm
Abbe condenser NA 0.9
Teaching set / Teaching unit side by side for 2 person
Epi- fluorescence attachment / CI-FL Epi-fluorescence Attachment (4 filter cubes mountable)
ND4/ND8/ND16 filters, Noise Terminator mechanism
FITC, TRITC, DAPI, TxRed
Digital camera / High-Definition Cooled Color Camera DS-Fi1c, 2/3 in. high density CCD; total number of pixels: 5.24 million
Exposure Time: 130 μsec to 60 sec
Recordable Pixels: 2560 x 1920, (5.6fps- 23 fps)
Exposure control: Auto / Manual
Exposure correction:13 steps
Digital zoom:10 to 1200%
Storage format: BMP, TIFF, JPEG, JPEG2000 etc., selectable in NIS-Elements
Interface: USB device port × 1 (Printer, PTP support, Vendor unique / switching)
USB host port × 2 (USB mouse, USB memory stick, USB keyboard, bar code reader, microscope connection),
External sync input/output, Camera I/F × 1
Digital Sight DS-L3 camera controller with large built-in 8.4" 1024 x 768 touch-screen monitor
Improved Image Processing Performance
Software / Microscope imaging software geared for acquisition and device control for standard research applications, requiring four-dimensional imaging.
Photo documentation, Selectable Layout Interface, Acquisition Color Setting
Data Management and Various databases and tables can easily be created and images can be saved to the database via one simple mouse click.
Report Generation, Image Quantification, include object counting, automated measurements, region of intent measurements and time measurements including image rationing of multi-channel images.
Image Segmentation. Automatic Measurement records length, area, angle and colorimetry. Automatic Measurement and interactive Measurement.
Classifier allows segmentation of the image pixels according to different user-defined classes, and is based on different pixel features such as intensity values.
Morphology. Large Image Stitching

5) Digital microscope n=8

Optical System: / CFI60 Infinity Optical System
Coarse/Fine Focusing: / Coaxial coarse/fine focusing, Focusing stroke: 30mm, Coarse: 9.33mm/rotation, Fine: 0.1mm/rotation, Minimum reading: 1um
Coarse motion torque adjustable, Refocusing function
Illumination: / High luminescent White LED Illuminator (Eco-Illumination), AC adapter (100-240V)
Eyepiece Tube: / Ergonomic Binocular Tube (for F.O.V 22mm, inclination:10-30°, extension 40mm (when optional DSC port is attached, observation/photo: 100/0, 50/50)
Eyepiece Lens: / 10×(F.O.V.: 22mm),
Nosepiece: / Sextuple nosepiece
Stage / Ceramic-coated Stage without specimen holder (for 1slide (opens to left) can be attached)
Cross travel 54 (Y) × 78 (X) mm, with calibrations, stage handle height and torque adjustable
Observation Method: / Brightfield
Objectives / Plan Achromat 4X N.A. 0.10, W.D. 30.0 mm
Plan Achromat 10X N.A. 0.25, W.D. 10.5 mm
Plan Achromat 20X N.A. 0.40, W.D. 1.2 mm
Plan Achromat 40X N.A. 0.65, W.D. 0.56 mm
Condenser / Condenser focusing stroke: 27mm
Abbe condenser NA 0.9
Digital camera / High-Definition Color CameraDS-Fi2 with 2/3 in. high-density CCD; 5.24 megapixels (effective 5.07 megapixels)
Recordable Pixels: 2560 x 1920, 640 x 480 (max. 21 fps)
Exposure control: Auto / Manual
Exposure correction:13 steps
Digital zoom:10 to 1200%
Storage format:BMP, TIFF, JPEG, JPEG2000 etc., selectable in NIS-Elements
Interface:USB device port × 1 (Printer, PTP support, Vendor unique / switching)
USB host port × 2 (USB mouse, USB memory stick, USB keyboard, bar code reader, microscope connection),
External sync input/output, Camera I/F × 1
Exposure Time: 130 μsec to 60 sec
Digital Sight DS-L3 camera controller with large built-in 8.4" 1024 x 768 touch-screen monitor
Improved Image Processing Performance
Software / Microscope imaging software geared for acquisition and device control for standard research applications, requiring four-dimensional imaging.
Photo documentation, Selectable Layout Interface, Acquisition Color Setting
Data Management and Various databases and tables can easily be created and images can be saved to the database via one simple mouse click.
Report Generation, Image Quantification, include object counting, automated measurements, region of intent measurements and time measurements including image rationing of multi-channel images.
Image Segmentation. Automatic Measurement records length, area, angle and colorimetry. Automatic Measurement and interactive Measurement.
Classifier allows segmentation of the image pixels according to different user-defined classes, and is based on different pixel features such as intensity values.
Morphology. Large Image Stitching

6) Microscope n=6

7) Tissue Vacuum Infiltration Processor n=4

Characteristics / Specifications /
Capacity / Up to 300 cassettes
Reagent reservoirs / 13 (incl. waste and cleaning)
Paraffin Reservoirs / 4
Waste Containers / 2 (1 reagent and 1 paraffin wax)
Bulk Reservoirs / 2 (10.8L each)
Reagent / 3
Management / including paraffin wax
Level sensors / Level 4 Sensors
(150-cassettes, 300-cassettes,
cleaning and overfill).
External drain & fill:1 fill/drain and 2 fill ports
Display & Control / 10.4” color LCD, touch screen
Dimensions / 61 x 63 x 133 cm(W x D x H)
Weight / 170kg
Voltage / 230V ± 10%, 50/60 Hz, 12 Amps
External interface / UPS connection, external alarm output, Ethernet port

8) Cassette printer n=12

Characteristics / Specifications /
Throughput / Up to 900 cassettes per hour
Dimensions / up to W x D x H 46 x 65 x 94 cm
Weight / up to 30 kg
Capacity / Cassette Printer 6 magazines, 80 cassettes each
Printer driver, print resolution / Printer Driver Windows® based
Print Resolution Up to 360 dpi
Characters 1-D and 2-D barcodes, graphics, alphanumeric androman characters

9) Pathology Grossing Station n=5

Characteristics / Specifications /
Dimensions, width/depth/height / 152*74*122 cm
Dimensions with Base legs, width/depth/height / 152*74*203
Voltage / 230V, 50/60Hz
Weight, excluding base legs / up to 130kg
Grossing station facilities / Integral sink with mixing faucet
Easy access control panel
Ample lighting
Formalin container with spigot
C-fold paper towel holder
Magnetic instrument holder
Outside ventilation source
Base leg assembly
Camera mount facility
Eyewash assembly
Filter, activated carbon
Glove box holder
Microphone on Flex arm
Monitor and keyboard station
Scale, Economy Grade
Scale, Digital
Valve air
Video camera Arm
Video and dictation mounts
Voice activated dictation

10) Tissue embedding console system n=4

Characteristics / Specifications /
Embedding console, width, depth, height / 59*62*38 cm
Cryo console, width, depth, height / 33*59*38 cm
Cooling plate / 31*38 cm
Embedding console weight / 20 kg
Cryo console weight / 20 kg
Paraffin chamber capacity / 4 l
precisely metered and adjustable gravity feed paraffin dispenser
Thermal chamber capacity / 1.8l
Cooling plate capacity / 60 cassettes
Temperature range paraffin chamber / 50-70ºC
Thermal chamber temperature range / 50-70ºC
Hot plates and forceps wells temperature range / 50-70ºC
Cooling plate temperature range / -10-0ºC
Operation / Fully programmable ON/OFF control automatically start up and shuts down
Easy to read LED display
Accessories / Magnifying glass
Foot pedal switch
Electric forceps

11) Spectrophotometer n=1