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Breakthrough Invasive Candidiasis on Micafungin

Supplementary Material

FKS gene sequencing information by Candida species

Organism / Gene / Accession no. / Nucleotide regions sequenced
C. albicans / FKS1 / XM_716336 / 2340-2919 and 4151-5100
C. dubliniensis / FKS1 / GQ342611 / 2340-2919 and 4151-5100a
C. glabrata / FKS1 / XM_446406 / F1651 – R2225 and F3749– R4397
C. glabrata / FKS2 / XM_448401 / F1600 – R2513 and F3448– R4796
C. krusei / FKS1 / EF426563 / F1450 – R2590 and F3418 – R4600
C. parapsilosis / FKS1 / EU221325 / 1463-2466 and 3764-4708
C. tropicalis / FKS1 / EU676168 / 1752–2194 and 3888–4304a

aEquivalent to C. albicans (XM_716336)


C. glabrata MLST

Strain / MLST Allele / Sequence Type (STs) / Notes
FKS1 / LEU2 / NMT1 / TRP1 / UGP1 / URA3
Patient 8
Blood #1 / 5 / 7 / 8 / 7 / 3 / 6 / 3 / Most common ST found in the MLST database
Patient 8
Blood #2 / 5 / 7 / 8 / 7 / 3 / 6 / 3
Wild Type Reference Strain ATCC 90030 / 8 / 4 / 3 / 5 / 1 / 2 / 10 / Same ST match as Dodgson et al. JCM 2003, 41(12);5709-5717

C. tropicalis MLST

Strain / MLST Allele / Sequence Type (STs) / Notes
ICL1 / MDR1 / SAPT2 / SAPT4 / XYR1 / ZWF1a
Patient 11
Blood / 1 / 6 / 3 / 5 / 5 / 3 / 6 / Unusual ST; only matched 2 of 179 other submitted strains to the pubmlst.org database
Patient 11
Pleural Fluid / 1 / 6 / 3 / 5 / 5 / 3 / 6
Wild Type Reference Strain ATCC 750 / 1 / new / 9 / 5 / new / 1 / new / ST was not found in the MLST database