Online Resource3.Phylogenetic Analysis of 11Gene Familiesthat Catalyze the Synthesis And

Online Resource3.Phylogenetic analysis of 11gene familiesthat catalyze the synthesis and degradation of sucrose during sugar metabolism in Vitisvinifera.Maximum likelihood phylogenic analysiswas performed by Mega5.05(Tamura et al., 2011)with the JTT amino acid substitution model, a discrete gamma model with 5 categories.Bootstrapping was performed with 1000 replicates.

A: Sucrose synthase (SUSy); B: Sucrose phosphate synthase (SPS); C: Sucrose-phosphatase (SPP); D: Invertase (INV); E: Fructokinase (FRK); F: Hexokinase (HK); G: Glucose-6-phosphate isomerase(G6PIMase); H: UDP-glucose pyrophosphorylase (UDP-GPPPase); I: Phosphoglucomutase (PPGase); J: Phosphofructokinase (PPFTK); K: Pyrophosphate-dependent 6-phosphofructose-1-kinase (PDPPFTKase).

Online Resource3A:Phylogenetic analysis of Sucrose synthase(SUSy) in Arabidopsis and Vitisvinifera. SUS from Acaryochloris marina(NC_009925) also added to the analysis.

Online Resource3B: Phylogenetic analysis of Sucrose phosphate synthase (SPS) in Arabidopsis and Vitisvinifera.SPS fromProchlorococcusmarinus str. MIT 9313 (NP_896092) also added to the analysis.

Online Resource3C:Phylogenetic analysis of Sucrose-phosphatase (SPP) in Arabidopsis and Vitisvinifera.SPP from Microcoleusvaginatus FGP-2 (ZP_08495240) also added to the analysis.

Online Resource3D:Phylogenetic analysis of Invertase (INV) in Arabidopsis and Vitisvinifera. INV from Zymomonasmobilis(Q60115) also added to the analysis.

Online Resource3E:Phylogenetic analysis of Fructokinase(FRK) in Arabidopsis and Vitisvinifera.All the genes in Arabidopsis were annotated as pfkB-type carbohydrate kinase. pfkB-type carbohydrate kinasefrom Eubacterium rectalATCC (YP_002937307) also added to the analysis.

Online Resource3F:Phylogenetic analysis of Hexokinase (HK) in Arabidopsis and Vitisvinifera. pfkB-type carbohydrate kinase family protein from EubacteriumrectalATCC (YP_002937307) also added to the analysis.

Online Resource3G:Phylogenetic analysis of Glucose-6-phosphate isomerase(G6PIMase) in Arabidopsis and Vitisvinifera. G6PIMase from Mycoplasmamycoides subsp.capri LCstr. 95010(YP_004400191) also added to the analysis.

Online Resource3H:Phylogenetic analysis of UDP-glucose pyrophosphorylase(UDP-GPPPase) in Arabidopsis and Vitisvinifera. UDP-GPPPase from Sphingomonaspaucimobilis (AF461178) also added to the analysis.

Online Resource3I:Phylogenetic analysis of Phosphoglucomutase (PPGase) in Arabidopsis and Vitisvinifera. Phosphoglucomutase (PGM); PPGase fromBacillus subtilis (P18159) also added to the analysis.

Online Resource3J:Phylogenetic analysis of Phosphofructokinase (PPFTK) in Arabidopsis and Vitisvinifera.PPFTK from Nocardiopsisdassonvillei subsp. DassonvilleiDSM 43111 (ADH68530) also added to the analysis.

Online Resource3K:Phylogenetic analysis of Pyrophosphate-dependent 6-phosphofructose-1-kinase (PDPPFTKase) in Arabidopsis and Vitisvinifera. PDPPFTKase from Propionibacteriumfreudenreichii subsp. shermanii(P29495) also added to the analysis.