Butylated Hydroxyanisole

(BHA)

Hazardous Substances Policy - Assessment

CHEMICAL HAZARD AND RISK ASSESSMENT

School of Biosciences

Name of supervisor / Dr. C. Sweet / Assessment Number* / 1906
Signature
Assessor / Mr. E.W.A. Brydon / Date of Assessment / 27.09.2001

Signature

NotesA School COSHH form in Word is available on the School Server.

Available from the Health and Safety Unit.

Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.

Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.

Use a continuation sheet to expand any section of this form in hard copy version.

1 / LOCATION OF THE WORK ACTIVITY / S105, S106B
2 / PERSONS WHO MAY BE AT RISK
List names where possible / All members of Dr. C. Sweets group present and future; currently Mr. E. W. A. Brydon,
Dr. S. J. Morris, Miss. I. Y. Pappworth, Miss K. Baluch, Dr. M. Kirby, Miss K.. Ball and Mr . N. Sharif, and future
M.Res. or 3rd year project students.
3 / ACTIVITY ASSESSED / Butylated Hydroxyanisole (BHA) inhibition of virus-induced apoptosis.
4 / MATERIALS INVOLVED / Attach copies of data sheet(s)
NAME / AMOUNT
max/stored / HAZARD / RISK PHRASES / HAZDAT NO*** / BIOSCIENCESNO***
Butylated Hydroxyanisole / 5g / Toxic. / 2645
(BHA) / May cause cancer.
Harmful / by inhalation, contact with
skin and if swallowed.
Possible
sensitizer. / Target Organ: Liver
5 / INTENDED USE**
Give brief details and attach protocol/instructions / To be used as a tissue culture medium supplement, pre- and post
virus incubation, on cell monolayers in order to inhibit apoptosis. Weighed amounts will be diluted in absolute
ethanol to concentrations under 1M in tissue culture medium in the class 2 safety cabinet.
6 / RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding mothers especially at risk?
Acute Effects: Harmful if swallowed, inhaled or absorbed through skin. Causes eye and skin irritation. Material is
irritating to mucous membranes and upper respiratory tract. Prolonged or repeated exposure may cause allergic
reactions in certain sensitive individuals.
Chronic Effects: Carcinogen. Target Organ – Liver.
Toxicity Data. Oral – Rat LD50 = 2gm/kg, Intraperitoneal – Rat LD50 = 881mg/kg, ORL – Mouse LD50 =
1100mg/kg, Oral – Rabbit LD50 = 2100mg/kg.
Target Organ Data.
Behavioural (Altered sleep time) (Ataxia)
Lungs Thorax or Respiration (Respiratory stimulation)
Blood (Haemorrhage)
Incompatible with strong oxidising agents. Hazardous combustion or decomposition products: CO, CO2.
Only selected of RTECS data presented here. For full information RTECS # SL1945000
OEL = MAK
Vapour density – 6.2
Flashpoint >230F (>110degreesC)
Autoignition Temp. 599F (315degreesC).
7 / CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
Risk level is acceptable provided control measures are followed.
8 / CONTROL MEASURES
Additional to Good Chemical Practice
Wear compatible chemically-resistant gloves, face mask and lab coat when weighing out in a closed
microbalance. Make dilutions in ethanol (combustible solvent) in class 2 safety cabinet.
9 / INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
None.
10 / MONITORING
Performance of control measures,
None
Personal exposure / Health Surveillance
None
11 / WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
Discard BHA dissolved in ethanol into non-halogenated waste for disposal.
12 / REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
5 years.
13 / EMERGENCY ACTION
TO CONTROL HAZARDS / To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
Max spillage equates to 5g therefore for spillage wear lab coat, respirator mask and rubber gloves. Cover with dry
lime or soda ash and sweep up. Hold in a closed container for waste disposal avoiding the raising of dust.
Ventilate area and wash spill site after material pick up.
For spillage when dissolved in solvent wear protective equipment as above. Soak solvent spill up with paper towels and discard these and any used for clean afterwards into yellow bags for special waste.
Fire fighting measures:
Extinguishing media – water spray. Carbon Dioxide, dry chemical powder or appropriate foam. Emits toxic fumes under fire conditions – evacuate and inform fire department of risk.
TO PROTECT PERSONNEL / Evacuation, protection for personnel involved in clean-up, Special First Aid
Avoid inhalation, contact with eyes, skin or clothing. In case of contact immediately flush eyes or skin with
copious amounts of water for at least 15 minutes while removing contaminated clothing and shoes. Assure.
adequate flushing by separating the eyelids with fingers. Call a physician.
If swallowed, wash out mouth with water provided the person is conscious, call a physician.
If inhaled, remove to fresh air. If breathing becomes difficult call a physician.
TO RENDER SITE OF EMERGENCY SAFE / Clean-up/decontamination
See section 13.
CONTACT PHONE

10.10.00

* Prefix T is used for Teaching Assessment Number.

** Please include amount of chemicals used and how.

***Hazdat No is the UNICOSHH datasheet report number.

Biosciences No is the Biosciences data sheet number.

UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.

BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.