Cyprus Organization for the Promotion of Quality Cyprus Accreditation Body

CYPRUS ORGANIZATION FOR THE PROMOTION OF QUALITY
CYPRUS ACCREDITATION BODY

ACCREDITATION CERTIFICATE no.L026-2

The Board of Governors

of the Cyprus Organization for the Promotion of Quality

acting as the authorized Cyprus Accreditation Body

according to the Article 7 of the Law 156(Ι)/2002

grants accreditation to

CV SAFEFOOD LABORATORIES LTD

in Limassol

which has been assessed according to the Accreditation Criteria for Testing Laboratories as defined in the standard

CYS EN ISO/IEC 17025:2005

as competentto perform the Methods defined in the Scope of Accreditation referred to in the Annex of this certificate; the said Annex represents inextricable part of the certificate. The Accreditation Scope can only be modified after a decision of the Cyprus Accreditation Body.

The current Accreditation Certificate, no. L026-2,is issued on the 12thJanuary 2016 and is valid till the 19th December 2018.

Accreditation was granted for the first time on the 20th December 2010.

Antonis Ioannou

Director

Date: 12th January 2016

This laboratory is accredited in accordance with the recognised International Standard ISO/IEC 17025:2005. This accreditation demonstrates technical competence for a defined scope and the operation of a laboratory quality management System (ISO-ILAC-IAF Communiqué, 08/01/2009).

/ Annex
of the Accreditation Certificate number L026-2

Scope of Accreditation

οf the

CV SAFEFOOD LABORATORIES LTD

Valid as from the 20th December 2014 to the 19th December 2018

*Valid as from the 12th January 2016 to the 19th December 2018

Materials / Products / Type of testing /
Countable properties / Methods / Techniques
Enumeration of Heterotrophic Bacteria in Water (Membrane Filtration Method) using Yeast Extract Agar incubated at 22oC, 30oC and 37oC. / Modified method
WT SFL 01
based on the “Microbiology of DrinkingWater (2012), Part 7 - Methods for the enumeration of heterotrophic bacteria. Methods for the examination of Waters and Associated Materials, Environment Agency, UK”.
Drinking, surface and swimming pool water / Detection and Enumeration of Coliform Bacteria using Membrane Lactose Glucuronide Agar (Membrane Filtration Method) incubated at 37oC.
Confirmed using Lactose Peptone Water. / WT SFL 02C (C)
based on the “Microbiology of DrinkingWater (2009), Part 4 - Methods for the isolation and enumeration of coliform bacteria and Escherichia coli (including E. coli O157:H7). Methods for the Examination of Waters and Associated Materials, Environment Agency, UK”.
Detection and Enumeration of Coliform Bacteria using Membrane Lactose Glucuronide Agar (Membrane Filtration Method) incubated at 37oC. / WT SFL 02P (P)
based on the “Microbiology of DrinkingWater (2009), Part 4 - Methods for the isolation and enumeration of coliform bacteria and Escherichia coli (including E. coli O157:H7). Methods for the Examination of Waters and Associated Materials, Environment Agency, UK”.
Detection and Enumeration of Escherichia coli using Membrane Lactose Glucuronide Agar (Membrane Filtration Method) incubated at 37oC.
Confirmed using Lactose Peptone Water and Indole Production. / WT SFL 03C (C)
based on the “Microbiology of DrinkingWater (2009), Part 4 - Methods for the isolation and enumeration of coliform bacteria and Escherichia coli (including E. coli O157:H7). Methods for the Examination of Waters and Associated Materials, Environment Agency, UK”.
Detection and Enumeration of Escherichia coli using Membrane Lactose Glucuronide Agar (Membrane Filtration Method) incubated at 37oC. / WT SFL 03P (P)
based on the “Microbiology of DrinkingWater (2009), Part 4 - Methods for the isolation and enumeration of coliform bacteria and Escherichia coli (including E. coli O157:H7). Methods for the Examination of Waters and Associated Materials, Environment Agency, UK”.
*Detection and Enumeration of Pseudomonas spp. in Water using Pseudomonas C.F.C Agar (Membrane Filtration Method).
Confirmed using Oxidase reagent. / Modified method
WT SFL 04C (C)
based on “the Microbiology of Drinking Water (2010), Part 8 - Methods for the isolation and enumeration of Aeromonas species and Pseudomonas aeruginosa by membrane filtration. Methods for the examination of water and associated materials, Environment Agency, UK”.
Drinking, surface and swimming pool water / Detection and Enumeration of Pseudomonas aeruginosa in Water (Membrane Filtration Method) using Pseudomonas C.N Agar.
Confirmed using Milk Cetrimide Agar. / WT SFL 05C (C)
based on the “Microbiology of Drinking Water (2010), Part 8 - Methods for the isolation and enumeration of Aeromonas species and Pseudomonas aeruginosa by membrane filtration. Methods for the examination of water and associated materials, Environment Agency, UK”.
Detection and Enumeration of Pseudomonas aeruginosa in Water (Membrane Filtration Method) using Pseudomonas C.N Agar. / WT SFL 05Ρ (P)
based on the “Microbiology of Drinking Water (2010), Part 8 - Methods for the isolation and enumeration of Aeromonas species and Pseudomonas aeruginosa by membrane filtration. Methods for the examination of water and associated materials, Environment Agency, UK”.
Detection and Enumeration of Enterococcus spp. in Water (Membrane Filtration Method) using Slanetz and Bartley Agar.
Confirmed using Bile Aesculin Agar. / WT SFL 06C (C)
based on the “Microbiology of DrinkingWater (2012) Part 5 - A method for the isolation and enumeration of Enterococci by membrane filtration. Methods for the Examination of Waters and Associated Materials, Environment Agency, UK”.
Detection and Enumeration of Enterococcus spp. in Water (Membrane Filtration Method) using Slanetz and Bartley Agar. / WT SFL 06P (P)
based on the “Microbiology of DrinkingWater (2012) Part 5 - A method for the isolation and enumeration of Enterococci by membrane filtration. Methods for the Examination of Waters and Associated Materials, Environment Agency, UK”.
Detection and Enumeration of Legionella pneumophila and Legionella spp.(Membrane Filtration Method).
Identification by serological methods using Latex agglutination covering Legionella pneumophila serogroup 1, serogroups 2-14 and Legionella spp. / Modified method
WT SFL 08C (C)
based on “BS 6068: Part 4: Section 4.12: 1998. Detection and enumeration of Legionella, UK”.
Drinking, surface and swimming pool water / Detection and Enumeration of Legionella spp.in Water (Membrane Filtration Method). / Modified method
WT SFL 08P (P)
based on “BS 6068: Part 4: Section 4.12: 1998. Detection and enumeration of Legionella, UK”.
Detection and Enumeration of Sulphite Reducing Anaerobes (Clostridia spp.), including the enumeration of Spores, in Water (Membrane Filtration Method). / WT SFL 09
based on the “Microbiology of DrinkingWater (2010) Part 6 - Methods for the isolation and enumeration of Sulphite Reducing Clostridia and Clostridium perfringens by membrane filtration. Methods for the examination of water and associated materials. Environment Agency, UK”.
Detection and Enumeration of Clostridium perfringens, including the enumeration of Spores, in Water (Membrane Filtration Method).
Confirmed using Buffered Nitrate Motility Medium, and Lactose Gelatin Medium. / WT SFL 10C (C)
based on the “Microbiology of DrinkingWater (2010) Part 6 - Methods for the isolation and enumeration of Sulphite Reducing Clostridia and Clostridium perfringens by membrane filtration. Methods for the examination of water and associated materials. Environment Agency, UK”.
Detection and Enumeration of Clostridium perfringens, including the enumeration of Spores, in Water (Membrane Filtration Method). / WT SFL 10Ρ (P)
based on the “Microbiology of DrinkingWater (2010) Part 6 - Methods for the isolation and enumeration of Sulphite Reducing Clostridia and Clostridium perfringens by membrane filtration. Methods for the examination of water and associated materials. Environment Agency, UK”.
Detection and Enumeration of Staphylococcus spp. in Water (Membrane Filtration Method). / In-house method
WT SFL 07
adopting Membrane filtration of 100ml of Water and enumeration of Staphylococcus spp. using Mannitol Salt Agar incubated at 37°C for 48 hours.
Detection and Enumeration of Legionella pneumophila and Legionella spp. in Water (Direct Membrane Filtration Method with Low Bacterial Counts). / Modified method
WT SFL 11C (C)
based on “BS EN ISO 11731-2: 2008. Detection and enumeration of Legionella. Part 2 Direct Membrane Filtration Method for Waters with Low Bacterial Count”.
Drinking, surface and swimming pool water / Detection and Enumeration of Thermotolerant Coliform Bacteria using Membrane Lactose Glucuronide agar (Membrane Filtration Method). / WT SFL 12C (C)
based on the “Microbiology of DrinkingWater (2009), Part 4 - Methods for the isolation and enumeration of coliform bacteria and Escherichia coli (including E. coli O157:H7). Methods for theExamination of Waters and Associated Materials, Environment Agency, UK”.
Detection and Enumeration of Thermotolerant Coliform Bacteria using Membrane Lactose Glucuronide agar (Membrane Filtration Method). / WT SFL 12P (P)
based on the “Microbiology of DrinkingWater (2009), Part 4 - Methods for the isolation and enumeration of coliform bacteria and Escherichia coli (including E. coli O157:H7). Methods for the Examination of Waters and Associated Materials, Environment Agency, UK”.
Food
(excluding liquids and dry products) / Enumeration of Total Viable Count using the bioMérieux Tempo TVC Automated Most Probable Number Technique.
30oC / FT SFL 01TP
based on “BioMérieux Tempo TVC, REF 80 007”.
TEMPO Biomerieux Validation Certificate, AFNOR BIO 12/15-09/05.
Enumeration of Total Coliform Count using the bioMérieux Tempo TC Automated Most Probable Number Technique. / FT SFL 02TP
based on “BioMérieux Tempo TC, REF 80 006”.
TEMPO Biomerieux Validation Certificate, AFNOR BIO 12/17-12/05.
Enumeration of Escherichia coli Count using the bioMérieux Tempo EC Automated Most Probable Number Technique. / FT SFL 03TP
based on “BioMérieux Tempo EC, REF 80 004”.
TEMPO Biomerieux Validation Certificate, AFNOR BIO 12/13-02/05.
Enumeration of Coagulase Positive Staphylococci (Staphylococcus aureus) using the bioMérieux Tempo STA Automated Most Probable Number Technique. / FT SFL 04TP
based on “BioMérieux Tempo STA, REF 80 002”.
TEMPO Biomerieux Validation Certificate, AFNOR BIO 12/28-04/10.
Enumeration of Enterobacteriaceae Count using the bioMérieux Tempo EB Automated Most Probable Number Technique. / FT SFL 05TP
based on “BioMérieux Tempo EB, REF 80 003”.
TEMPO Biomerieux Validation Certificate, AFNOR BIO 12/21-12/06.
Food (excluding liquids and dry products), and Environmental Swabs / Enumeration of Listeria monocytogenes in Food and Environmental Samples (Oxoid Listeria Precis™ protocol). / FT SFL 09RAP
AFNOR Validation Certificate UNI 03/05-09/06 (Enumeration).
Detection of Salmonella spp. in Food and Environmental Samples (BioRad RAPID’Salmonella short protocol). / FT SFL 10RAP
AFNOR Validation Certificate BRD 07/11-12/05.
Enumeration of presumptive Bacillus cereus (Colony count technique at 30°C using AES BACARA chromogenic agar). / FT SFL 11RAP
based on BS EN ISO 7932:2006. AFNOR Validation Certificate AES 10/10-07/10.
Enumeration of Campylobacter spp. (Colony count technique at 41.5°C using CASA chromogenic agar). / FT SFL 13RAP
based on ΙSO/TS 10272-2:2006. Microval Validation Certificate RQA2010LR38.
Enumeration of Clostridium perfringens (Colony count technique at 37°C). / FT SFL 14
based on “BS EN ISO 7937:2004. Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of Clostridium perfringens - Colony count technique”.
Fish and Fish Products / Detection of Vibrio parahaemolyticus (selective isolation on TCBS and Colorex Chromogenic Vibrio). / FT SFL 15
based on “DD ISO/TS 21872-1:2007. Microbiology of food and animal feeding stuffs - Horizontal method for detection of potentially enteropathogenic Vibrio spp. - Part 1: Detection of Vibrio parahaemolyticus and Vibrio cholerae”.

Note:In cases where the code for a method includes the indication C or P, this refers to a confirmed or presumptive method respectively. These two alternatives are included in the scope provided that there is an adequate communication and understanding by the customer on their meaning as well as a clear reference on the test reports, in line with the requirements of the relevant policy of the Cyprus Accreditation Body.

Authorised persons to sign test reports are Dr Constantinos A. Vorkas and Mr. Charles Fuller.

General Remarks

This Annex refers only for tests carried out in the premises of the Laboratory, at the following address: 14 Mattheou str., Ayios Athanasios Industrial Zone, CY - 4104, Limassol, Cyprus.

Antonis Ioannou

Director

Date: 12th January 2016

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