Biology 595 ExperimentAditi Trehan
Dr. Nancy Pelaez
Objective:
To determine whether the wind chill, caused by the suction, played an important role in the drop in temperature of the animal.
Protocol:
Experiment #1:
Set up the vacuum and suction equipment and measured the change in temperature by positioning the probe of the thermometer closer to the suction. Below, I have provided a diagram that summarizes the experiment.
Experiment #2:
Set up a beaker with water and positioned the probe inside the beaker. Using the suction, I withdrew the water and measured the change in temperature. Below, I have provided a diagram that summarizes the experiment.
Experiment #3:
Designed an experiment in which I found another way to measure the change in temperature. The problem we had from the earlier experiments was that as the water was withdrawn, I saw that the temperature dropped because the probe was exposed to the outside environment.
Therefore, I design an experiment where the water level wouldn’t decrease as the water was being sucked out. Below, I have provided a diagram for the experimental design.
Results and Discussion:
In experiment #1, the change in temperature was measured that was caused by the suction. However, I didn’t see any considerable change in the temperature. In experiment #2, I measured the change in temperature but I set up a different experimental design for the experiment. I wanted to replicate the effect of suction on the temperature. Thus, I set up an experiment where the provided suction withdrew the water and the temperature was measured simultaneously. The results clearly show that there were no noteworthy changes in the temperature. The following are the results for the second experiment.
Time * (minutes) / Temperature Change (degree Celsius)0 / 21
1 / 21
2 / 21
3 / 21
4 / 21
5 / 21
6 / 21
7 / 21
8 / 21
9 / 21
10 / 21
* The time is the moment after 3:40pm. For example, Time 0 is 3:40pm; Time 1 is 3:41pm etc.
In experiment 3, I designed this experiment where there would be constant flow of water through the beaker as the water would be sucked out. This experimental design mimics the constant flow in the myometrium experiment. Additionally, the probe wouldn’t be exposed to the outside environment. In the earlier experiment, a decrease in temperature was observed when the probe was exposed to the outside environment as the water was withdrawn from the beaker. There was no observation of a significant temperature change in experiment 3. This might have been because of maintaining the constant flow of solution. The temperature was 37 degrees.
Conclusion:
Looking and analyzing these 4 experiments, we can conclude that the suction didn’t cause the decrease in the temperature of the animal. Although, the Pasteur pipette was responsible for the suctioning was extremely cold – it didn’t play a very significant role in the decrease of the temperature in the animal.
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