Supplementary Data

Table SI. Segregation of BC1 populations from three crosses.

Cross B73/zb7 a Mo17/zb7a zong31/zb7 a
Numbers of green plants 334 123 120
Numbers of zebra plants 318 114 108
Total numbers 652 237 228
x2 0.39 0.34 0.63

aThe male partner for the cross; bgreen plants and zebra plants were determined by visual inspection. x23.8; cP0.05 was considered as significant.

Table SII. The PCR-based molecular markers designed for fine mapping.

Type of Marker Marker Primer Pairs Fragment Restriction
Size Enzyme
SSR umc1534 5' GACCCCAAATCTCTCCTTCCTC 3' 92 bp
5' TAGCTAAGCTTGTGCTTGCTCG 3'
umc2189 5' CGTAAGTACAGTACACCAATGGGC 3' 143 bp
5' ACACCGACTACAAGCCTCTCAACT 3'
AC209902 5' CGCCTTGAGACACATGGATAGA 3' 151 bp
5' TGCTGTTCACTGGCAGTAGATTC 3'
AC191530 5' GCCGCATTGAACAGGAGAAAAGGAC 3' 268 bp
5' CCACACCCATACCGCCAAACCC 3'
AC196186 5' CTGCGTCCATTCGTATTGTATCGT 3' 230 bp
5' AACATCGCACTCTCTCAGCCAAC 3'
AC190915 5' CCATGACATTTAATTCGGGTTT 3' 235 bp
5' GGGTGGAGTTTTCCTTCTTTC 3'
AC202035 5' TTCCCACATGGACATGGTTA 3' 203 bp
5' TCTCCCTTGGCCCTTTATC 3'
AC209801 5' CGGGCATCCAACCACCATT 3' 263 bp
5' ATTGTCCTCGTTATCCTCGTGACTC 3'
CAPS CAPS1 5' ACATATGCCTCTAAACACGCCAT 3' 179 bp Alu1
5' CTCGGTTTTGGTTGATGGCT 3'
CAPS2 5' GATTAACACCCTCTTTATTGC 3' 749 bp Bsh12361
5' ATAACCACAAATGAGCCTCTA 3'
CAPS3 5' AGTGCTAAATCCATCATTCCCAGTT 3' 311 bp MaeⅡ
5' TGACCAGCCTCTGCTACCTCC 3'
CAPS4 5' TTTGCCTGTTCATCCGTCTCA 3' 475 bp Alu 1
5' CGCCGTCTAGGTTCTGTTCGT 3'

Figure S1. The chloroplast (MEP) and cytosol (MVA) pathways for isoprenoid biosynthesis in plants. Enzymes and isoprenoids of the MEP pathway: DXS, 1-deoxy-D-xylulose-5-phospate synthase; DXR, 2C-methyl-D-erythritol-4-phosphate synthase; CMS, 4-diphosphocytidyl-2C-methyl-D-erythritol 4-phosphate synthase; CMK, 4-diphosphocytidyl-2C-methyl-D-erythritol kinase; MCS, 2C-methyl-D-erytrithol-2,4-diphosphate synthase; HDS, 2C-methyl-D-erytrithol 2,4-cyclodiphosphate reductase; HDR, 1-hydroxy-2-methylbutenyl-4-diphosphate reductase; IPP, isoprenoid diphosphate; DMAPP, dimethylallyl diphosphate; GPP, geranyl diphosphate; GGPP, geranylgeranyl diphosphate; GA, gibberellic acid; ABA, abscisic acid. Enzymes and isoprenoids of the MVA pathway: ATOT, acetoacety1-CoA thiolase; HMGS, hydroxymethylglutaryl-CoA synthase; HMGR, hydroxymethylglutaryl-CoA reductase; MK, mevalonate kinas; PMK, phosphomevalonate kinase; MPD, mevalonatepyrophosphate decarboxylase; FPPS, farnesyl diphosphate; FPP, farnesyl diphosphate.

Figure S2. Phenotypic identification of zb7 mutant at different stages. A, 3-leaf old plant; B, 9- —to 10-leaf plant; C, adult plant.

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