DIVISION OF LABORATORY ANIMAL RESOURCES Form #: T- 102

Training and Orientation For Non-Rodent Species

DATE: / PROTOCOL NUMBER:
BUILDING:
ANIMAL HOUSING / SPECIES:
P.I. NAME: / P.I. EMAIL:
PERSONNEL: / PERSONNEL EMAIL:
LAB PHONE # / PERSONNEL ACCESS ID
How long will you work in the lab? (months/years) / Will you require 24 hr access to the animal facility?
  1. Please complete the responsibility section below, have the PI sign and take with you when you meet with DLAR Husbandry /VSS.
  2. Schedule and complete building orientation, contact information is below. Be sure to print the form at take it with you.
  3. Personnel may be asked protocol questions specific to the species being used. For surgical procedures, review page 3 to assist with knowing what is expected. It is expected you will read the protocol to assure a good understanding of your role in it and answer the pertinent questions related.

Who is responsible for the following / Personnel
Listed above / DLAR/
VSS / Who is responsible for the following / Personnel
Listed above / DLAR /
VSS
Handling / Restraint / Pre-Operative care
Injections / Post-Procedure Care
Blood Collection- / Aseptic -survival surgery
Anesthesia- / Aseptic -- non-survival surgery
Ventilator use / Analgesia
Transport from room / O.R. Charting / Monitoring
Transport to lab / Gavage – Feeding tube
Identification / Breeding - Sexing
Physical Exam / Euthanasia / Assurance of Death
Recovery Period / Enrichment

PI/Lab Trainer Signature: ______

DLAR VSS Staff Signature:______Date:______

BUILDING LEADER INFORMATION *Working at the Elliman building may require orientation with both DLAR and VSS
ScottHall / Kathy / 577-1114 /
Biological Science / VAMC / Carrie / 577-5278/577-0371 / 576-1000x65880x63433 /
KEI / iBio / Star / 577-1690 or 577-9983 / 577-1637 /
MottCenter / Tracy/Susan / 577-1757 () (Susan )

EllimanBuilding / Laura / 577-8516 /
VeterinarySurgicalServices / Janine / 577-1130 /
Lande–MRB / Tracy / 577-1277 /
ApplebaumPharmacy / Serene / 577-1631 /
DATE: / DLAR FACILITY:
PI NAME: / PERSONNEL NAME:
BuildingLeader: Pleasecomplete applicable areas to your building,signand returnto DLAR Training ASAP.
LocationofDLAR/VSSOffice / DiscussCensus/ Tracking sheets / LocationofTreatmentRoom
LocationofDLAR/VSScontactinfo / DiscussPost-OPcare and supplies / View surgical areas in area
Locationof“ReportingProblems”
sign / Cagesetup(bedding,food, nesting,
etc...) / Identifycagewash(clean/dirty)
andcommonsuppliesused
Showbuildingalarmlocations / Discuss flags used in PI room / Discusstransportinganimals
outsidecage/roomorDLAR
IdentifythePI’sanimalroom/PI’s animal cages (if multiple in room) / ReviewCageCard–requirements / View recovery / holding areas
Reviewpostingsonthedoor / Update PI Lab Emergency contacts / LocationofDeadanimals
Discuss species behavior / View imaging areas / Weekend/holiday responsibilities
Review hazard procedures (hazard information in protocol, marking cages, signage, communication with DLAR staff, handling cages/animals). Provide commonly used hazard sheet.

PersonnelSignature: ______DLAR Signature:______

UPDATED EMERGENCY PHONE NUMBERS FOR CURRENT PROTOCOL(S) WITH DLAR

VSS signature:______

Commonly Used Hazards

Biologic Hazards / Chemical Hazards / Hazard Identification Steps
1.Communicate with the DLAR technician in the room/facility about your planned experiment with the hazardous agent.
  1. Recommend the communication occurs 1-2 days prior to initiating experiment.
2.Obtain the proper hazardous sticker in advance of the experiment.
3.Label the cage card at the time of the inoculation with the sticker and the following information:
  1. Date
  2. Compound administered
  3. Route of administration
  4. Dose

Human tumor cell lines (immunocompromised mice) / Streptozotocin (STZ) (diabetes induction)
Pseudomonas aeruginosa / Chemotherapeutic agents (cancer research)
Adeno-associated virus (AAV) / LPS (sepsis research)
Candida albicans / Tamoxifen (gene expression, chemotherapeutic)
DMSO
Novel test compounds (generally identified by letters or numbers, not commercially available)

Please note that new employees responsible for performing surgery must understand aseptic technique. One must demonstrate their ability throughout the procedure in an acceptable manner.

IACUC Website:

Blackboard:

  1. ASEPTIC TECHNIQUES IN SURGERY
  • View the surgical procedures area
  • Designated area within the lab, low traffic/noise (rodents only)
  • DLAR facility-procedure rooms, low traffic/noise (rodents only)
  • DLAR Facility-Veterinary Surgical Services operating room
  • Within a Biosafety hood
  • Review equipment set up and sanitation of area
  • Schedule surgery time to allow for adequate recovery and monitoring before leaving for the day.
  • Prior to surgery, what pre-operative steps are performed?
  • Obtain baseline weight
  • Check supplies needed
  • Administer analgesics
  • Check working status of all equipment to be used
  • Schedule area to be used (if shared)
  • Autoclave surgical supplies
  • Animal surgical cite preparation, ocular lubricant and anesthesia induction
  • Gather required surgeons attire (gloves, cap, mask, scrubs, lab coat)
  • What does the surgical area contain?
  • Sterile drape for base of surgical area/table
  • Heat source (warm water blanket, microwave pad)
  • Anesthesia machine, ventilator, special equipment
  • Sterilized instrument pack
  • Hot bead sterilizer (rodent surgery)
  • During operative procedures, what care and/or techniques will be performed?
  • Monitor respirations, heart rate, temperature (if applicable)
  • Monitor gas or injectable anesthesia depth
  • Method of incision closure (suture, wound clips, staples, glue)
  • Supportive fluids or drug administration
  • Non-survival procedure
  • What care is administered during the post-anesthesia phase?
  • Additional ophthalmic ointment applied
  • Supportive fluids, analgesics
  • Heat support under ½ of recovery cage(rodents) or under larger animals
  • Recovery cage placed in a low traffic area.
  • Rodents-No bedding used, lay animal on paper towel and replace if soiled.
  • Larger Animals- padded, head should face front of cage.
  • Monitor individual animals.
  • Rodents- every 5-10 minutes, switching from right/ left recumbency every 10 minutes, return to housing room when they can ambulate on their own and eat/drink normally.
  • Larger Animals- every 15 minutes, switch from right/ left recumbency every 30 minutes. Return to housing room when fully recovered (ambulate on their own and eat/drink normally)
  • Supplemental food given when recovered.
  • Recommend moist palatable food to stimulate appetite.
  • Should be placed in cage so the animal does not have to expel a great amount of energy to get to it.
  • What post-operative recovery and monitoring is performed?
  • Recovery period is monitored until sutures, wound clips or staples are removed.
  • Heart rate, respirations, temperature (if applicable)
  • Incision – appearance, closure condition, signs of infection.
  • Urine and Feces – normal appearance and amount
  • Appetite – appears to be eating and drinking normally, monitor for weight loss.
  • Behavior – social, ambulatory, stance
  • Appearance – hair coat, eyes, posture
  • Bandages/harness/e-collar/etc… -correct fit, change/replace schedule
  • Documentation and charting – all items documented using acceptable medical terms without added emoticons, personal feelings or slang words. Documentation is a regulated part of all research studies and must be complete or it is assumed that care and oversight was not attended to.
  • What adverse outcomes that may occur secondary to the procedure?
  • Problems or side effects with techniques, drugs or equipment

  1. IACUC WEBSITE – Policies, Guidelines and Procedures
  • All staff should view the IACUC website to become familiar with Policies, Guidelines and Procedures.
All members working within an approved protocol should understand the level of responsibility the PI accepts as listed in the standard operating procedure
  1. Additional information
  • Personnel can always use dead animals, they make good practice patients. Contact DLAR at your building and ask to be notified when they are euthanizing animals (rodents). You will benefit because…
  • You can practice new techniques or master older ones.
  • These animals will not count against the protocol
  • There are no charges to the PI’s account.
  • There are no amendments needed to match species, strain, sex, age, etc…
  • Responsibility lies on the staff to seek training and opportunities to perfect their skill.
  • The PI is responsible for all non-compliance incidents involving their staff.
  • Be forthright about areas of concern that may affect research animals, testing results and medical outcomes. (Wrong injections, miscalculated drugs, contamination of specimens, etc...).
  • The protocol must be followed as written (amendments included). Talk to veterinarian to discuss protocol deviations and amendments ASAP, this may decrease approval time.
  • New personnel and students coming into the lab require mentoring and teaching. DLAR is here to help in this process
  • Review DLAR Training on Blackboard

Institutional Animal Care and Use Committee (IACUC)

TRAINING REQUIREMENTS CHECKLIST

Training Requirements: All laboratory personnel must complete the appropriate training before working on an animalprotocol unsupervised.

REQUIRED PRIOR TO PROTOCOL or PERSONNEL AMENDMENT SUBMISSION

Collaborative Institutional Training Initiative (CITI) Program Modules (

  • Working with the IACUC (a refresher course is required every 3 yrs)
  • Responsible Conduct of Research
  • Animal Allergy Exposure Reduction
  • Select all of the species that will be used on this protocol
  • Minimizing Pain and Distress (if the protocol includes mice or rats)
  • Aseptic Surgery (for staff who perform survival surgery on any species)
  • Animal Contact Occupational Health & Safety Program
  • AniCon Questionnaire (Form #1080)

REQUIRED PRIOR TO PROTOCOL or PERSONNEL AMENDMENT FINAL APPROVAL

  • Division of Laboratory Animal Resources (DLAR)

*To be eligible for DLAR training all CITI training must be completed and an AniCon Questionnaire filed with OEHS.

Once confirmed, the IACUC office (577-1629) will contact the DLAR Trainer () to arrange

training.

  • Building Orientation
  • Species Specific Training
  • Aseptic Techniques Training (for staff who perform survival surgery)
  • Laboratory Housing Training (for staff who will take care of animals outside of the DLAR facilities >12 hrs)

Office of Environmental Health and Safety (OEHS)

  • Laboratory Safety Training
  • Biosafety Training (if applicable)
  • Basic Radiation Safety Training (if applicable)

(DLAR training/training request form/rev.10-2013)

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