Supplementary Table 1: Examination of the EC Numbers Which Are Unique in Humancyc

Supplementary table 1: Examination of the EC numbers which are unique in Humancyc

EC numbers / genes / comment
1.1.1.181 / Q9H2F3 / annotated as 1.1.1.- in other database. Reaction already in our database.
1.1.1.249 / P49366 / New annotation is 2.5.1.46, EC number removed
1.1.1.81 / Q9UBQ7 / mutigunction as 1.1.1.79 and 1.1.1.81
1.1.1.88 / P04035 / should be 1.1.1.34
1.1.1.94 / many genes / should be 1.1.1.8, produce only NADH rather than both NA and NP
1.12.7.2 / Q96S10 / no known function
1.17.4.2 / Q7LG56 / should be 1.17.4.1
1.2.1.1 / P11766 / 1.1.1.1 & 1.1.1.284
1.2.1.16 / P00352 / 1.2.1.36 in other databases
1.2.1.8 / P49189 / multifunction as 1.2.1.47, 1.2.1.3, 1.2.1.19
1.3.1.72 / Q15392 / as 1.3.1.-, 24-dehydrocholesterol reductase but not delta24-sterol reductase
1.3.99.1 / O75609 Q99643 / hucyc is right, uniprot has enz name but no EC number, kegg annotate as 1.3.5.1
1.4.3.10 / P27338 / as 1.4.3.4 amine oxidase. 1.4.3.10 (putrescine oxidase) is a subfunction of 1.4.3.4
1.8.4.2 / P35754 / no EC in uniprot, 1.8.4.2 is a wrong EC number for the reaction in humancyc
2.1.1.126 / Q9NVM4 / Protein arginine N-methyltransferase 7, link to old uniprot ID but the EC and reaction in hucyc are right
2.1.1.156 / Q14749 / 2.1.1.20
2.4.1.227 / O15294 / 2.4.1.-, wrong EC number
2.4.1.87 / 3 genes / 2 are activator (GTA) or kinase (CDC2L2), another real enzyme (ENSG00000165196) has been removed in Ensembl
2.7.1.12 / gene only in Ensembl without any description of function, wrong in hucyc.
2.7.7.18 / Q96T66 / should be 2.7.7.1
2.7.8.8 / P48651 / wrong, the gene actually catalyze a reaction to produce PhCholine but not directly from CDPDAG
3.1.2.4 / Q92931 / hucyc is right, uniprot has enz name but no EC number
3.1.3.53 / O60237 / actually a regulatory protein
3.1.4.46 / Q9NZC3 / 3.1.4.44 in uniprot, hucyc is right
3.2.1.37 / O76044 / right but without any reaction in hucyc, uniprot same annotation but fragment
3.4.11.14 / P55786 / right but without any reaction in hucyc, as 3.4.11.- in uniprot
3.4.99.46 / no reaction in hucyc
3.5.1.1 / Q8IV80 / no function known in uniprot
3.6.1.14 / P18859 / 3.6.3.14
3.6.1.21 / Q9UKK9 / 3.6.1.13 in other databases, hucyc is right, should be ADP sugar pyrophospatase rather than ADPribose pyrophastase
4.3.1.1 / removed gene in both ensembl and uniprot
4.6.1.10 / removed gene
5.1.2.3 / P40939 / general reaction, multigunction gene, other Ecs in uniprot
6.3.2.12 / Q05932 / should be tetrahydrofolate synthase (6.3.2.17) rather than dihydrofolate synthase