BIO 209 Microbiology Lab Schedule

Spring Semester 2007

Date / Lab / Exercises / Review/Data / Assignment
1/10-11

1/15-16
1/17-18 / 1
2 / Introduction to course; Syllabus review; Safety;
Microscopy
MARTIN LUTHER KING HOLIDAY
Exp. 4 Microscopy cont’d
Prepared Slides
http://www.mansfield.ohio-state.edu/~sabedon/black
03/.htm / xiii-xvi
29-36
1/22-23 / 4 / QUIZ ( #1)
Exp. 8 Smear Preparation
Exp. 9 Simple Staining
Exp. 10 Negative Staining / 4 / 53-70
1/24-25 / 5 / PRE-LAB QUIZ (#2)
Exp. 11 Gram Stain
Exp. 12 Acid-Fast Stain
http://medlib.med.utah.edu/WebPath/INFEHTML/
INFEC033.html
http://www.geocities.com/capecanaveral/3504/gallery.
htm?20063 / 8,9,10 / 71-82
1/29-30 / 6 / QUIZ (# 3)
Exp. 13A: Spore Stain
Exp. 13B: Capsule Stain / 11,12 / 83-92
1/31-2/1 / 7 / Exp. 1 Culturing Microbes
Exp. 2 Colony Isolation
Exp. 15 Media and pp. 93-5 / 13A, 13B / 1-12
13-20
101-107
2/5-6 / 8 / Exp. 21 Microbial Growth Curve
Indirect Method; Dilution Problems
Assign: VIRTUAL LAB http://www-micro.msb.le.ac.uk/labwork/bact/bact1.htm / 2,15 / 135-142
2/7-8 / 9 / Exp. 21 Microbial Growth Curve
Dilution Problems
In Class Activity / 21 / 135-142
2/12-13
2/14-15 / 10
11 / EXAM I (labs 1-9)
Exp. 16 Temperature
Exp. 17 pH / Exam / Study
109-116
2/19-20 / 12 / Exp. 18/19 Atmospheric Requirements
Subculture and Gram Smear Unknown Organism / 16,17 / 117-128
2/21-22 / 13 / Exp. 22,23,24 TSI, Enzymes,
Carbohydrates / 18,19 / 143-160
2/26-27 / 14 / Inoculate IMViC/H2S/Urease/Nitrate
QUIZ (# 4) Discuss Lab 13 / 22-24 / 143-160
2/28-3/1 / 15 / Discuss IMViC/H2S/Urease/Nitrate
Crossword Puzzles
Assign: VIRTUAL LAB http://www.hhmi.org/biointeractive/
Click on “Bacterial Id. / 25-29 / 161-178
185-189
3/5-6 / 16 / Technical Evaluation
Exp 30/31 Catalase/Oxidase
Video: PCR
QUIZ (#5) Virtual Lab & Lab 15 / 25-29 / 189-196
3/7-8 / 17 / EXAM II (labs 11-16)
(Includes Gram Stain and Acidfast Stain)
UNKNOWN LABORATORY REPORT
DUE
3/12-16 / SPRING BREAK
3/19-20 / 18 / VIDEO: History of Antibiotics
VIDEO: Evolutionary Arms Race
ASSIGN LIBRARY REPORT
3/21-22 / 19 / Exp. 44 Antimicrobial
Susceptibility Testing
VIDEO: Bacterial Resistance / 279-288
3/26-27 / 20 / PRE-LAB QUIZ (#6)
Exp. -- Transformation (h’out)
Exp 58 Conjugation / 44 / Handout
379-384
371-372
3/28-29 / 21 / Exp 41 Moist Heat
Exp 42 Osmotic Pressure
Exp 43 Radiation / 58
Transformation / 261-278
4/2-3 / 22 / Exp 46 Disinfect/Antiseptics
Assign: VIRTURAL LAB-Ames Test
http://itg1.meteor.wisc.edu/wxwise/kti/bacteria/virtualames.html / 41,42,43
Transformation / 293-301
4/4-5 / 23 / Exp 69 Urine cultures
Exp 47 Milk Quality
Exp 51 Water Analysis: A / 46
/ 447-452
303-308
321-325
4/9-10 / 24 / Exp 51 Water Analysis: B
Exp 62/63 Mouth/Throat/Skin
Biofilm Exercise-see Purple Manual pp. 151-155 / 47,51,69 / 321-330
403-414
4/11-12 / 25 / Exp 51 Water Analysis: C
Exp 67 Enterotube/API-20E
VIDEO: Chlamydia trachomatis
QUIZ (# 7) / 51,62,63 / 321-330
403-414
433-442
4/16-17 / 26 / Exp 64 Staphylococcus
Exp 65 Streptococcus
Exp 66 S.pneumoniae
Assign: VIRTUAL LAB-SEROLOGY
www.physioex.com (Quiz grade) / 415-432
4/18-19 / 27 / Review Staph and Strep
VIDEO: Rational Antibiotic Prescribing
QUIZ (#8) -Staph, Strep, Serology
Assign: Virtual DNA Fingerprinting Lab
http://www.pbs.org/wgbh/nova/sheppard/analyze.
html
LIBRARY REPORT DUE / 64,65,66 / Virtual
Serology
Lab
4/23-24 / 29 / DNA Fingerprinting Worksheet DUE (Quiz grade)
Course Evaluation
Video: World Within a World (Parasitology)
Video: Maximum Containment Laboratory / P. Manual
pp.205-238
4/25-26 / 30 / EXAM III (labs 18-30)
(Includes Gram Stain and Acidfast Stain) / STUDY


Laboratory exercises may be modified at the discretion of the coordinator. Advanced notice will be given if possible.