Supplement 1. Primers used for quantitative real-time RT-PCR
Target gene / SequenceCOX-2 / F:AGG TCA TTG GTG GAG AGG TG
R: GGT TCT CAG GGA TGT GAG GA
EP receptor 1 / F: GCT CCT TGC GGC ATT AGT GTG
R: TGC GGT CTT TCG GAA TCG T
EP receptor 2 / F: CGT TAT CCT CAA CCT CAT TCG C
R: TCC GTC TCC TCT GCC ATC G
EP receptor 3 / F: TTG CTG GCT CTG GTG GTG AC
R: GCT GGA CTG CGA GAC GGC
EP receptor 4 / F: TGA CCC AAG CAG ACA CCA CCT
R: TCC CAC TAA CCT CAT CCA CCA A
IFN-γ / F: ACA AAG ATG GCA GAG CAC GA
R: TCC ACC AAC ATG TGC GGT TT
TNF-α / F: AGC DGA TGG GTT GTA CCT TGT CTA
R: TGA GAT AGC AAA TCG GCT GAC GGT
IL-1β / F: AAG GGC TGC TTC CAA ACC TTT GAC
R: ATA CTG CCT GCC TGA AGC TCT TGT
IL-6 / F: TGG CTA AGG ACC AAG ACC AT
R: TAA CGC ACT AGG TTT GCC GA
IL-12 / F: TGA TGA TGA CCC TGT GCC TTG GTA
R: ATT CTG AAG TGC TGC GTT GAT GGC
IL-17 / F: TCC ACC GCA ATG AAG ACC CTG ATA
R: ACC AGC ATC TTC TCG ACC CTG AAA
GAPDH / F: TCA ACA GCA ACT CCC ACT CTT CCA
R: ACC CTG TTG CTG TAG CCG TAT TCA
COX-2: cyclooxygenase-2, EP receptor: eicosanoid-prostanoid receptor, INF- γ: interferon- γ,
TNF- α: tumor necrotizing factor α, IL: interleukin, GAPDH: glyceraldehyde-3-phosphate
dehydrogenase
Supplement 2. Flow cytometric analysis of IL-17+ expression in activated T cells after mixed lymphocyte reactions used in this study.
After DE induction, 16 corneas from DE-induced group were secured, and CCR7+CD11b+ and CCR7-CD11b+ cells were isolated by cell sorting FACS analysis. IFN-γ-IL-17-CD4+ T cells were harvested from submandibular and cervical LNs of normal condition group and sorted by FACS analysis. To measure T cell activation, each CCR7+CD11b+ and CCR7-CD11b+ cells were thoroughly washed and subsequently co-cultured with IFN-γ-IL-17-CD4+ T cells. After 5 days of incubation, samples were stimulated with PMA/ionomycin for 10 h with GolgiPlug. Responder T cells were collected for subsequent flow cytometry analyses of intracellular IL-17 expression. After permeabilization, intracellular cytokine levels were measured with anti- mouse IL-17-FITC antibodies.