Request for Services
Requesting Investigator
Gene of Interest: / Date:Investigator’s Name:
Last / First / M.I.
Email Address: / Phone/FAX:
Mailing Address:
Service Requested:
☐ / Gene Targeting in mice or rats / ☐ / loxP insertion / ☐ / AAV Vector Construct
☐ / Editing/Knockout / ☐ / Transgene Knock-in / ☐ / AAV Vector Construct & custom prep
Is a specific genetic background needed? If yes, please specify: ______
Project Description (describe the rodent line required or the virus desired):
Relevance of this project to diabetes research:
Potential Known Complications (Has the gene of interest been targeted previously? If so, are there known or suspected developmental concerns associated with the loss of gene function?):
Service Timelines
Project phase / Time FrameInitial Consultation
- Gene Analysis
- CRISPR Guide design
- Targeting DNA design
Construction of gRNA templates
Zygote injection for toxicity and guide testing in blastocysts / 2-4 weeks
- Construction of DNA targeting templates
Zygote Injection of gRNA, Cas9 and targeting construct to generate modified mice / 4-8weeks
- PCR screening of founder DNA
Sequence analysis of targeted mutation / 2-3 weeks
- Transfer to requesting investigator
Rates/Fees
Knockout/Editing: $6780loxP insertion/Transgene Knock-in: $7460
AAV viral vector construction: $1250
AAV viral vector construction and custom prep: $3490
**A 30% surcharge for IDC coverage is also applied to all requests from external, non-UM clients.
**Viral constructs with potentially broad application to diabetes research may be generated at no cost to the donating investigator if s/he agrees to share the reagent with other UM-MDRC investigators.
Confirmation of Request
The Molecular Genetics Core will design the targeting strategy, build the necessary reagents and interface with the UM Transgenic Animal Core for embryo injection to perform guide RNA validation in blastocysts (30 embryos) and subsequent generation of founder animals (300 embryos). Animals born following embryo transplantation will be screened by PCR for the presence of the requested genetic change. Once identified, potential founder animals will be made available to the requesting investigator for subsequent breeding and analysis. Given the variability in genetic targeting, the MGC cannot guarantee generation of founder animals or transmission of the altered allele from the founder animal to its offspring. Validation of the effects of any gene alteration is the responsibility of the requesting investigator.Request Approved By:
Status of Request: / ☐ / Accepted / ☐ / Declined
Are you an MDRC member? / ☐ / Yes / ☐ / No
Do you have an approved animal protocol for mice?
☐ / Yes, the protocol number is: ______
☐ / No (The MGC will not generate mice for investigators without an approved animal protocol for mice.)
Short code to be charged (UM investigators):______or PO # (external): ______
If federal funds will be used for this project, please list the grant number: ______
University of Michigan MGC Signature of Approval / Date
Requesting Investigator Signature of Approval / Date
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