Supplementary Table 1 Sequence of Primers Used

Supplementary Table 1 Sequence of primers used

Name / Sequence
Mouse FBP1 RT-PCR
mFBP1-F
mFBP1-R / CCATCATAATCGAACCTGAG
CTTCTCAGAAGGCTCATCAG
Mouse GAPDH RT-PCR
mGAPDH-F
mGAPDH-R / AAATGGTGAAGGTCGGTGTG
TGAATTTGCCGTGAGTGGAG
Human FBP1 RT-PCR
hFBP1-F
hFBP1-R / ACAGCAGTCAAAGCCATCTC GGTTCCACTATGATGGCGTG
Human GAPDH RT-PCR
hGAPDH-F
hGAPDH-R / GGAGTCAACGGATTTGGT GTGATGGGATTTCCATTGAT
Human GAPDH RT-PCR
hGAPDH-F
hGAPDH-R / GGAGTCAACGGATTTGGT GTGATGGGATTTCCATTGAT
FBP1 MSP
MSP-F
MSP-R / GATTTAAGTAGGCGGAGTCGC ACGAACCGCGAAACCTAACG
FBP1 USP
USP-F
USP-R / AGATTTAAGTAGGTGGAGTTGT
ATACAAACCACAAAACCTAACA
FBP1 BGS
BGS-F
BGS-R / GTTTGGTTTGGTTTAGTTGTATT
GAAATAACTTTAACTACTATACAAAA

Supplementary Table 2

Clinicopathologic features of FBP1 methylation in gastric cancer

Characteristics / Methylated / Unmethylated / p
n / % / n / %
Age
Mean ± SD / 65.7 ± 10.8 / 65.7 ± 13.9 / 0.999
Gender
M / 21 / 37.5 / 35 / 62.5 / 0.141
F / 11 / 23.9 / 35 / 76.1
H. pylori infection
Positive / 13 / 39.4 / 20 / 60.6 / 0.147
Negative / 7 / 22.6 / 24 / 77.4
Lauren Type
Diffuse / 10 / 32.3 / 21 / 67.7 / 0.663
Intestinal / 11 / 27.5 / 29 / 72.5
Differentiation
Poor / 15 / 36.6 / 26 / 63.4 / 0.111
Well or moderate / 6 / 19.4 / 25 / 80.6
TNM stage
I / 2 / 12.5 / 14 / 87.5 / 0.210
II / 4 / 22.2 / 14 / 77.8
III / 12 / 40.0 / 18 / 60.0
IV / 7 / 25.0 / 21 / 75.0

Supplementary Figure legends:

Supplementary Figure 1 FBP1 was downregulated in gastric cancer cell lines. FBP1 expression in gastric cancer cell lines and normal stomach tissues were determined by quantitative real-time RT-PCR as in Figure 2A.

Supplementary Figure 2 Nf-KB siRNA mediated Nf-KB depletion. Nf-KB specific siRNA were transfected into MKN28 cells. Two days later, whole cell lysates were collected for Western blotting. GFP specific siRNA was used as the control siRNA.