Supplementary Table 1

Supplementary Table 1

Site characteristics, accession number and valerenic acid content in selected populations of V. jatamansi

S. No / Population / Altitude
(m asl) / Latitude
(N°) / Longitude
(E°) / Accession number / Number of genotype used in PCR
1 / Daulaghat / 1215 / 29 ° 46’ 00” / 80° 18’ 00” / 113261 / 7
2 / Katarmal / 1250 / 29° 38’ 25” / 79° 37’ 20” / BSD- 112793 / 6
3 / Tharali / 1330 / 30° 04’ 06” / 79° 30’ 08” / 113206 / 8
4 / Dolti / 1626 / 30° 02’ 56” / 79° 29’ 50” / GBPI-3201-2 / 5
5 / Berinag / 1672 / 29° 43’17” / 80° 02’ 14” / 113257 / 5
6 / Majkhali / 1702 / 29° 40’ 20” / 79° 31’ 47” / 113209 / 6
7 / Talwari / 1785 / 30° 01’ 46” / 79° 30’ 59” / 113205 / 8
8 / Khirshu / 1810 / 29° 51’ 17” / 79° 35’ 46” / 113212 / 5
9 / Didihat / 1850 / 29°, 46’16” / 80°17’ 59” / 113253 / 7
10 / Pithoragarh / 1872 / 29° 36’ 14” / 80°11’ 40” / 113214 / 9
11 / Sitlakhet / 1900 / 29° 35' 40" / 79°32'42" / GBPI-3201-1 / 6
12 / Gwaldam / 1923 / 30° 00’ 24” / 79° 33’ 30” / 113213 / 7
13 / Kausani / 1925 / 29° 47’ 51” / 79° 26’ 12” / 113210 / 8
14 / Ukhimath / 1985 / 30° 31’ 00” / 80° 05’ 00” / 113258 / 5
15 / Camel back / 2000 / 30° 27’ 43” / 78° 04’ 26” / 113200 / 5
16 / Jaberkhet / 2080 / 30° 27’ 18” / 78° 06’ 50” / 113260 / 7
17 / Joshimath / 2100 / 29°47'37" / 79°27'38" / 113211 / 4
18 / Dunagiri / 2125 / 29° 47’ 37” / 79 27’ 40” / 113254 / 6
19 / Buranskhanda / 2150 / 30° 27’ 22” / 78° 05’ 59” / 113207 / 6
20 / Nainital / 2176 / 29° 23’ 34” / 79° 27’ 45” / 113208 / 7
21 / Makku band / 2240 / 30° 34’ 0.0” / 79° 13’ 00” / 113262 / 7
22 / Munsyari / 2240 / 30° 03’ 39” / 80° 14’ 36” / 113202 / 6
23 / Malyadaur / 2350 / 30° 08, 16” / 79° 57’ 56” / 113255 / 3
24 / Dwali / 2730 / 30° 10, 38” / 79° 59’ 46” / 113204 / 5
25 / Surkanda / 2775 / 30° 24’ 21” / 78° 17’ 21” / 113259 / 3
Total / 151

Supplementary Table 2

Details of ISSR primers shown clear and reproducible banding patterns during analysis of Valeriana jatamansi

S. No. / Primer Name / Sequence (5’-3’) / Length (bp) / Annealing temperature
°C / Total amplified fragment / No. of polymorphic fragments
1 / HB 10 / GAGAGAGAGAGACC / 14 / 42 / 8 / 8
2 / 17898B / CACACACACACAGT / 14 / 44 / 11 / 11
3 / 17898A / CACACACACACAAC / 14 / 45 / 8 / 8
4 / 17899A / CACACACACACAAG / 14 / 45 / 12 / 12
5 / HB 11 / GTGTGTGTGTGTCC / 14 / 45 / 9 / 9
6 / HB14 / CTCCTCCTCGC / 11 / 45 / 5 / 5
7 / HB 13 / GAGGAGGAGGC / 11 / 45.6 / 5 / 5
8 / HB 15 / GTGGTGGTGGC / 11 / 46 / 9 / 9
9 / HB 12 / CACCACCACGC / 11 / 46.5 / 11 / 11
10 / HB 8 / GAGAGAGAGAGAGG / 14 / 51 / 11 / 11
11 / HB 9 / GTGTGTGTGTGTGG / 14 / 51 / 8 / 8
12 / 814 / CTCTCTCTCTCTCTCTTG / 18 / 52 / 5 / 5
13 / 844A / CTCTCTCTCTCTCTCTAC / 18 / 52 / 10 / 10
14 / UBC880 / GGAGAGGAGAGG AGA / 15 / 52 / 7 / 7
15 / UBC888 / BDBCACACACACACA CA / 17 / 52 / 6 / 6
16 / UBC 890 / VHVGTG TGT GTG TGT GT / 17 / 52 / 8 / 8
17 / UBC 810 / GAGAGAGAGAGAGAGAT / 17 / 55 / 5 / 5
18 / UBC840 / GAGAGAGAGAGAGAGAYT / 18 / 55 / 8 / 8
19 / UBC859 / TGTGTG TGTGTGTGT GRC / 18 / 55 / 6 / 6
20 / UBC860 / TGTGTGTGTGTGTGT GRA / 18 / 55 / 7 / 7

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