Supp. Table 1Analysis of Variance for comparison of trait sizes (R+L/2), among D. bipectinata, D. parabipectinata and their

hybrids

Sex –Trait / Source of variation / SS / df / MS / F
Female – SBN
/ Total / 58.379 / 199 / -
Between groups / 9.356 / 3 / 3.119 / 12.469*
Within groups / 49.023 / 196 / 0.250
Female- WL / Total / 4702.369 / 199 / -
Between groups / 3234.606 / 3 / 1078.202 / 143.979*
Within groups / 1467.763 / 196 / 7.489
Female-ON / Total / 7060.099 / 199 / -
Between groups / 142.594 / 3 / 47.531 / 1.347
Within groups / 6917.505 / 196 / 35.293
Male - SBN / Total / 79.020 / 199 / -
Between groups / 31.010 / 3 / 10.337 / 42.199*
Within groups / 48.010 / 196 / 0.245
Male - WL / Total / 2257.420 / 199 / -
Between groups
Within groups / 749.530
1507.890 / 3
196 / 249.843
7.693 / 32.475*
Male - SCTN / Total / 407.349 / 199 / -
Between groups / 19.444 / 3 / 6.481 / 3.275*
/ Within groups / 387.905 / 196 / 1.979

Note:*P<0.001; SBN-sternopleural bristle number; WL-wing length; ON- ovariole number; SCTN- sex comb tooth number

Supp. Table 2. Analysis of Variance for comparison of trait sizes (R+L/2), among D. bipectinata, D. malerkotliana and their

hybrids

Sex –Trait / Source of variation / SS / df / MS / F
Female – SBN
/ Total / 75.839 / 199 / -
Between groups / 9.974 / 3 / 3.325 / 9.893*
Within groups / 65.865 / 196 / 0.336
Female- WL / Total / 5559.389 / 199 / -
Between groups / 3765.554 / 3 / 1255.185 / 137.145*
Within groups / 1793.835 / 196 / 9.152
Female-ON / Total / 838.469 / 199 / -
Between groups / 295.784 / 3 / 98.595 / 35.609*
Within groups / 542.685 / 196 / 2.769
Male - SBN / Total / 76.949 / 199 / -
Between groups / 15.464 / 3 / 5.155 / 16.432*
Within groups / 61.485 / 196 / 0.314
Male - WL / Total / 4574.420 / 199 / -
Between groups
Within groups / 2697.380
1877.040 / 3
196 / 899.127
9.577 / 93.887*
Male - SCTN / Total / 1823.849 / 199 / -
Between groups / 1519.424 / 3 / 506.475 / 326.087*
/ Within groups / 304.425 / 196 / 1.553

Note:*P<0.001; SBN-sternopleural bristle number; WL-wing length; ON- ovariole number; SCTN- sex comb tooth number

Supp. Table 3. Analysis of Variance for comparison of trait sizes (R+L/2), among D. parabipectinata, D. malerkotliana and their hybrids

Sex –Trait / Source of variation / SS / df / MS / F
Female – SBN
/ Total / 70.299 / 199 / -
Between groups / 19.664 / 3 / 6.555 / 25.372*
Within groups / 50.635 / 196 / 0.258
Female- WL / Total / 28985.249 / 199 / -
Between groups / 1866.254 / 3 / 622.085 / 4.496*
Within groups / 27118.995 / 196 / 138.362
Female-ON / Total / 895.539 / 199 / -
Between groups / 75.534 / 3 / 25.178 / 6.018*
Within groups / 820.005 / 196 / 4.184
Male - SBN / Total / 96.649 / 199 / -
Between groups / 26.264 / 3 / 8.755 / 24.379*
Within groups / 70.385 / 196 / 0.359
Male - WL / Total / 2063.969 / 199 / -
Between groups
Within groups / 186.914
1877.055 / 3
196 / 62.305
9.577 / 6.506*
Male - SCTN / Total / 1434.375 / 199 / -
Between groups / 1110.845 / 3 / 370.282 / 224.323*
/ Within groups / 323.530 / 196 / 1.651

Note:*P<0.001; SBN-sternopleural bristle number; WL-wing length; ON- ovariole number; SCTN- sex comb tooth number

Supp. Table 4. Analysis of Variance for comparison of trait sizes (R+L/2), among D.bipectinata, D. pseudoananassae and their

hybrids

Sex –Trait / Source of variation / SS / df / MS / F
Female – SBN
/ Total / 83.520 / 199 / -
Between groups / 32.500 / 3 / 10.833 / 41.618*
Within groups / 51.020 / 196 / 0.260
Female- WL / Total / 5709.020 / 199 / -
Between groups / 4254.360 / 3 / 1418.120 / 191.077
Within groups / 1454.660 / 196 / 7.422
Female-ON / Total / 600.469 / 199 / -
Between groups / 108.214 / 3 / 36.071 / 14.362*
Within groups / 492.255 / 196 / 2.512
Male - SBN / Total / 76.177 / 167 / -
Between groups / 17.466 / 3 / 5.822 / 16.263*
Within groups / 58.711 / 164 / 0.358
Male - WL / Total / 2967.463 / 167 / -
Between groups
Within groups / 1818.965
1148.498 / 3
164 / 606.322
7.003 / 86.580*
Male - SCTN / Total / 1139.249 / 167 / -
Between groups / 656.764 / 3 / 218.921 / 74.413*
/ Within groups / 482.485 / 164 / 2.942

Note:*P<0.001; SBN-sternopleural bristle number; WL-wing length; ON- ovariole number; SCTN- sex comb tooth number

Supp. Table 5. Analysis of Variance for comparison of trait sizes (R+L/2), among D. parabipectinata, D. pseudoananassae and their hybrids

Sex –Trait / Source of variation / SS / df / MS / F
Female – SBN
/ Total / 47.151 / 183 / -
Between groups / 4.428 / 3 / 1.476 / 6.219*
Within groups / 42.723 / 180 / 0.237
Female- WL / Total / 874.957 / 183 / -
Between groups / 274.987 / 3 / 91.662 / 27.500*
Within groups / 599.969 / 180 / 3.333
Female-ON / Total / 873.038 / 183 / -
Between groups / 6.393 / 3 / 2.131 / 0.443*
Within groups / 866.645 / 180 / 4.815
Male - SBN / Total / 85.528 / 189 / -
Between groups / 25.284 / 3 / 8.428 / 26.021*
Within groups / 60.244 / 186 / 0.324
Male - WL / Total / 5374.238 / 189 / -
Between groups
Within groups / 483.028
4891.210 / 3
186 / 161.009
26.297 / 6.123*
Male - SCTN / Total / 830.170 / 189 / -
Between groups / 566.746 / 3 / 188.915 / 133.391*
/ Within groups / 263.424 / 186 / 1.416

Note:*P<0.001; SBN-sternopleural bristle number; WL-wing length; ON- ovariole number; SCTN- sex comb tooth numbe

Supp. Table 6. Analysis of Variance for comparison of trait sizes (R+L/2), among D. malerkotliana, D. pseudoananassae and their hybrids

Sex –Trait / Source of variation / SS / df / MS / F
Female – SBN
/ Total / 91.711 / 159 / -
Between groups / 38.586 / 3 / 12.862 / 37.769*
Within group / 53.125 / 156 / 0.341
Female- WL / Total / 1306.100 / 159 / -
Between groups / 288.910 / 3 / 96.303 / 14.769*
Within group / 1017.190 / 156 / 6.520
Female-ON / Total / 787.486 / 159 / -
Between groups / 270.461 / 3 / 90.154 / 27.202*
Within group / 517.025 / 156 / 3.314
Male - SBN / Total / 80.748 / 157 / -
Between groups / 29.600 / 3 / 9.867 / 29.706*
Within group / 51.149 / 154 / 0.332
Male - WL / Total / 1734.152 / 157 / -
Between groups
Within group / 217.328
1516.824 / 3
154 / 72.443
9.850 / 7.355*
Male - SCTN / Total / 305.343 / 157 / -
Between groups / 172.395 / 3 / 57.465 / 66.564*
/ Within group / 132.949 / 154 / 0.863

Note:*P<0.001; SBN-sternopleural bristle number; WL-wing length; ON- ovariole number; SCTN- sex comb tooth number