FROM

Dr.NISHMA P. NANAVATI

I M.D.

Dept. of Dravyaguna,

Shri Shivayogeeshwar Rural AyurvedicMedicalCollege & Hospital

INCHAL, Dist-Belgaum.

TO,

THE REGISTRAR

RajivGandhiUniversity of Health Sciences,Karnataka,

Bangalore.

THROUGH,

THE PRINCIPAL AND H.O.D. OF DRAVYAGUNA,

Shri Shivayogeeshwar Rural AyurvedicMedicalCollege & Hospital,

INCHAL, Dist- Belgaum.

Respected Sir,

Sub: Submission ofproforma for registration of subject for dissertation.

I humbly request you to kindly register the below mentioned subject against my name for the submission of dissertation to the RajivGandhiUniversity of health sciences, Karnataka, Bangalore for the partial fulfilment of M.D.(Ayu).

Title of Dissertation

“ EXPERIMENTAL EVALUATION OF VEDANASTHAPANA (ANALGESIC)

EFFECT OF KATPHALA (MYRICA NAGI Thunb.)”

Herewith I am enclosing completed proforma for registration of subject for dissertation.

Thanking You,

Date: 27/04/2012 Yours faithfully

Place:INCHAL

(Dr. NISHMA P. NANAVATI)

RAJIVGANDHIUNIVERSITY OF HEALTH SCIENCES,

KARNATAKA, BANGALORE.

ANNEXUREII

PROFORMA FOR REGISTRATION OF SUBJECT FOR DISSERTATION

1. NAME OF THE CANDIDATE: Dr.NISHMA NANAVATI

AND ADDRESS D/O. PUSHPENDRA NANAVATI

C-3, MAHAVIR FLATS,

SATYA SAI SCHOOL ROAD,

JAMNAGAR- 361 008

GUJARAT.

2. NAME OF THE INSTITUTE : SHRI SHIVAYOGEESHWAR RURAL

AYURVEDICMEDICALCOLLEGE

HOSPITAL, INCHAL-591102

DIST-BELGAUM.

3. COURSE OF STUDY : AYURVEDA VACHASPATHI

SUBJECT M.D. DRAVYA GUNA

4. DATE OF ADMISSION : NOVEMBER-2011

5. TITLE OF THE TOPIC :

“EXPERIMENTAL EVALUATION OF VEDANASTHAPANA (ANALGESIC)

EFFECT OFKATPHALA (MYRICA NAGI Thunb.)”

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6. Brief resume of the intended work:

6.1 Need for study :

The utility of Ayurvedic science is to maintain the health of a healthy individual and to cure the disease of a patient(1).

The mind and body together with the sense organs are the sites of manifestation of happiness and miseries(2). Pain is the common complaint which makes the patient to meet the Physician. The property of a drug that subdues pain (vedana) in a particular part of the body and which restores the normal state is known as vedanasthapana(3).

Pain, said to be one of the nature’s earliest signs of morbidity and is one of the commonest presentation seen in medical practice, which brings disturbance in equilibrium state of a person. In order to manage such condition there are good number of drugs available in medical field. Among those some are expensive and some are having potential side effects such as dyspepsia, gastrointestinal bleeding and platelet aggregation etc. found in drugs like aspirin(4).

The word Analgesic is derived from Greek word an – without and algia – pain. Analgesic drugs act in various ways on the peripheral and central nervous system. A drug or a medicine given to reduce the pain, without resulting in loss of consciousness is known as Analgesic(5).

In the present context inspite of various existing analgesics there is necessity of still better, safe and effective analgesic drugs. In the field of ayurveda, charaka – a well known physician has given 50 mahakashayas for single and compound drug therapies to different disease ailments. Among them he mentioned Vedanasthapana Mahakashaya(3).Nowadays so many drugs from these Mahakashayas are screened for their analgesic properties such as Kadamba, Shirisha, mocharasa etc. Such of the Vedanasthapaka dravyas when used, might help in the better management of pain. Katphala (Myrica nagi Thunb.) is one of them. Which is easily available and cost effective may satisfy the need of present day situation by acting as potent herbal analgesic drug. With this perspective this study is selected for the critical assesment of analgesic effect of Katphala on albino rats.

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6.2 Review of literature:

In Ayurveda it is clearly mentioned that ‘ vatadrute nasty ruja’ (6)indicating vata is the main causative factor for the manifestation of pain in the human body. The concept of vedanasthapana action is discussed in Charaka Samhita and Ashtanga Sangraha(7). And sushruta has described Katphala in Rodhradi, surasadi, parushakadi and lakshadi gana. Whereas parushakadi gana is mentioned as Anilvinashana(8).

The term pain is originally derived from the latin word ‘poena’, meaning punishment. The international association for the study of pain defines that “pain as an unpleasant sensory and emotional experience, arising from potential tissue damage”(9).

Katphala(Myrica nagi Thunb.) is a subtropical shrub commonly known as ‘boxberry’ belongs to Myricaceae family. In medicinal uses and chemical constituents of Myrica nagi Thunb.have been widely studied. It is a tree of medium height i.e.20-25 ft. It is found in hilly regions of Nepal and Northern parts of India especially in the region of Punjab, Gadwall, Kumaon etc(10).It has antispasmodic, anti-inflammatory, analgesic etc activities(11).Katphala is mentioned in many nighantus which possess properties like kashaya-tikta-katu rasas, ushna veerya and actions like kapha-vataghna. It is indicated in diseases like shwasa, arshas, jvara, gulma, meha etc(12),(13),(14).

Moreover to prove the above hypothesis, proper screening of classical literature, contemporary texts, previous works, research articles, internet, journals and periodicals will be done so as to get sufficient background information to carry out the study.

Previous work done :

  1. Dr. Durga A.R. – Study on Rodhradi Gana dravya w.s.r to Lodhra & Katphala in the management of Shweta pradara in 1999 at Dravya guna department, Dr.B.R.RGovernmentAyurvedaMedicalCollege, Vijayawada.
  2. Dr. Jyothi M. Kempannavar – Jwaraghna effect of Katphala and Usheera, A comparative study through experimental evaluation in 2006 at Dravya guna department, A L N Rao Ayurveda Medical College, Koppa, RGUHS.
  3. Dr. Arya Prabhu – An experimental evaluation of Caesalpinia bonducella Roxb. For its Vedanasthapana (analgesic) property in 2008 at Dravya guna department, Alva’s AyurvedicMedicalCollege, Moodbidri, RGUHS.

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6.3 Aims & Objectives of the study:

1. Pharmacognostical and phytochemical study of Myrica nagi Thunb.

2. To evaluate the vedanasthapana (analgesic) activity ofKatphala( Myrica nagi

Thunb.) in vivo method.

3. To compare the analgesic effect of test drug with the standard group.

7. Materials and Methods:

7.1 Source of data

1. Literary sources: Literary data will be collected on relevant aspects from textbooks,

journals, websites etc. on Ayurvedic and modern sciences.

2. Source of drug: Genuine, botanically identified Katphala will be collected from its

Natural habitat.

3. Preparation of the drug: Katphala churna is prepared as per the classical references.

4. Experimental source of data: The action of bark of Myrica nagiThunb.will be tested

inreference to standard (aspirin) and normal saline (control).

5.Place of work:Department of Dravya Guna, S.S.R.A.M.College, Inchal and

Recognised institute of pharmacy.

7.2 Method of Collection of data (including sampling procedures):

Experimental Study Design and Protocol
Sample / Wister albino rats
Inclusion Criteria / Healthy Wister albino rats weighing 180-200g.

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Exclusion Criteria / 1) Wister albino rats used for other experiments.
2) Diseased Wister albino rats.
Method / In vivo method
Pre-experimental setup / The animals will have free access to water and food and will be acclimatized at least 1 week before starting the experiments. The experiment will be performed under standard conditions of temperature, light, humidity and noise. Three groups of six Wister Albino rats each weighing between 180-200g will be used. They will be divided into three groups containing 6 animals in each group. All the animals will be weighed before the experiment.
Grouping / Groups / No.of Rats / Drug / Form / Purpose
Group I / 6 / Normal saline aline / Liquid / Normal Control
Group II / 6 / Aspirin / Powdered tablet mixed in Saline. / Standard Drug
Group III / 6 / Powder of bark of Myrica nagi Thunb. / Powder with suspension / Trial drug
Procedure / Groups of 6 rats of either sex with an initial weight of 180 to 200 g will be used for each dose. The hot plate will be used. The temperature will be controlled for 55° to 56 °C. And observations on animals of each will be recorded.
Dose / The dose is converted from human dose to animal dose according to the conversion formula, Rat dose/kg body weight = human dose x 0.018 x 5.
Duration / 8 days
Observations / a.The number of licking or jumping occurs will be recorded.
b.The latency of licking and jumping will be recorded by a stop-watch before and after 20, 60 and 90 min following oral administration of the standard, control and the trial drug.

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Calculations / The prolongation of latency time before and after the administration of trial drug will be calculated in reference to control and standard drugs.
Statistics / One way ANOVA test.

7.3 Does the study require any investigations or interventions to be conducted on patient or other humans or animals? If so please describe briefly.

Yes, this being an experimental study conducted on healthy albino rats that are maintained on standard food and water.

7.4Has ethical clearance been obtained from your institution in case of 7.3?

Yes, obtained. Ethical clearance certificate is enclosed.

8. List of referances:

  1. Chakrapanidatta, Charaka samhita, Sutra sthana, Chapter 30, Shloka 26, Yadavji Trikamji Acharya editor. Edition reprint 2004. Varanasi : Chaukhambha Sanskrit Sansthan; 2004. P. 187
  2. Agnivesa, Charaka Samhita, Shareerasthana, Vol.II, 1st chapter, Shloka 136, R.K.Sharma & Bhagwan Dash editor. 7th Edition. Varanasi : Chaukhambha Sanskrit Series office; 1994. P. 334.
  3. Agnivesha, Charaka Samhita, Sutra sthana, Vol-I, 4th chapter, 18th Shloka, R.K.Sharma & Bhagwan Dash editor. 7thEdition. Varanasi : Chaukhambha Sanskrit Series office; 2001. P.100.
  4. Harrison, Principles of Internal Medicine, Vol-I, Chapter 12, Anthony S. Fauci et al editors.14th ed. Singapore : McGraw– Hill Companies; 1998. P.55
  5. Website –
  6. Dalhanacharya, Sushruta samhita, Sutrasthana, Chapter 17, Shloka 7, Yadavaji Trikamji Acharya editor. Reprinted 4th ed. Varanasi : Chaukhambha orientalia; 1980. P. 83

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  1. Vagbhata, Astanga samgraha, Sutra sthana, Vol-I, 15th chapter, Shloka 43, Prof. K.R.Srikantha Murthy translater. 6th edition. Varanasi: Chaukhambha orientalia; 2002. P. 308
  2. Maharsi Susruta, Susruta samhita,Part-I, Sutrasthana, Chapter 38, Shloka 14,18,43,64;edited by Kaviraja Ambikadutta Shastri, Reprintedition 2007, Varanasi : Chaukhambha Sanskrit Sansthan;2007. P. 143
  3. Article D-cgac1345
  4. P.C.Sharma, M.B.Yelene and T J Dennis,Data Base on Medicinal plants used in Ayurveda, volume 8, New Delhi : Central Council for Research in Ayurveda and Siddha; 2007. P.207
  5. Dhanvantari, Dhanvantari Nighantu, Guduchyadi varga, Dr.Jarkhande Oza & Dr.Umapati Mishra Editor, 2004. Varanasi : Chaukhambha Surbharati Prakashan; 2004. P. 33
  6. Bhavamishra, Bhavaprakasha Nighantu, Haritakyadi varga, shloka 181, G.S.Pandey editor. 6th ed. Varanasi : ChaukhambhaBharatiAcademy; 1982. P.100
  7. Madanapala, Madanpala Nighantu, Haritakyadi varga, shloka 202,203; Ramprasad patiyala editor. Bombay : khemaraj Shrikrishnadas prakashan; 2004. P. 37
  8. Kaiyadeva, Kaiyadeva Nighantu, Oushadhi varga, Shloka 1137,1138; Prof Priyavrata Sharma & Dr.Guru Prasada Sharma editor. 1st ed.Varanasi : Chaukhambha orientalia; 1979. P.210

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9. Signature of the candidate :

(DR. NISHMA P. NANAVATI)

10. Remarks of the guide :

11. Names & Designation of :

11.1) Guide :DR. SHREEDEVI HUDDAR, M.D.(AYU)

Asst. Professor.

Dept. Of DRAVYA GUNA

S.S.R.A.M.College & Hospital

Inchal- 591102

11.2) Signature :

11.3) Co-Guide :DR. S.S.DEVALAPUR, M.D.(AYU)

Lecturer.

Dept. of DRAVYA GUNA

S.S.R.A.M.College & Hospital

Inchal – 591102

11.4) Signature :

11.5) Head of the department : Dr. Vamsi Krishna K. Gurjala, M.D.(AYU)

Professor & HOD

Dept. of DRAVYA GUNA

S.S.R.A.M.College & Hospital

Inchal- 591102

11.6) Signature :

12. Remarks of chairman :

& principal

12.1) Signature : (Principal/CMO)

(Dr. G VINAY MOHAN)