Supplemental materials

Bioprocess and Biosystems Engineering

Bioinspired enzymatic synthesis of silica nanocrystals provided by recombinant silicatein from the marine sponge Latruncilia oparinae

Y. N. Shkryl, V.P. Bulgakov, G.N. Veremeichik, S.N. Kovalchuk, V.B. Kozhemyako, D.G. Kamenev, I.V. Semiletova, Y.O. Timofeeva, Y.A. Shchipunov, Y.N. Kulchin

Suppl. Table 1 Technical parameters and results of the EDX analysis of silica crystals using a Shimadzu EDX-800HS-P instrument

Sample : 12/2 Crystals SiO2

Operator : Timofeeva

Comment : metal oxids

Group : SiO2 Std

Measurement Condition

Instrument : 800HS2 Atmosphere : Air Collimator : 10(mm) Spin : No
Analyte / TG / kV / uA / FI / Acq.(keV) / Anal.(keV) / Time(sec) / DT(%)
Si / Rh / 15 / 1000-Auto / ---- / 0 - 20 / 1.5 – 1.9 / Live - 99 / 14
C / Rh / 15 / 1000-Auto / ---- / 0 - 20 / 0.1 – 0.5 / Live - 99 / 14
N / Rh / 15 / 1000-Auto / ---- / 0 - 20 / 0.2 – 0.6 / Live - 99 / 14
O / Rh / 15 / 1000-Auto / ---- / 0 - 20 / 0.3 – 0.7 / Live - 99 / 14
Na / Rh / 15 / 1000-Auto / ---- / 0 - 20 / 0.8 – 1.2 / Live - 99 / 14
Mg / Rh / 15 / 1000-Auto / ---- / 0 - 20 / 1.1 – 1.5 / Live - 99 / 14
Al / Rh / 15 / 1000-Auto / ---- / 0 - 20 / 1.3 – 1.7 / Live - 99 / 14
P / Rh / 15 / 1000-Auto / ---- / 0 - 20 / 1.8 – 2.2 / Live - 99 / 14
S / Rh / 15 / 1000-Auto / ---- / 0 - 20 / 2.1 – 2.5 / Live - 99 / 14
K / Rh / 15 / 1000-Auto / ---- / 0 - 20 / 3.1 – 3.5 / Live - 99 / 14
Ca / Rh / 15 / 1000-Auto / ---- / 0 - 20 / 3.5 – 3.9 / Live - 99 / 141
Sc / Rh / 15 / 1000-Auto / ---- / 0 - 20 / 3.9 – 4.3 / Live - 99 / 14
Ti / Rh / 50 / 224-Auto / ---- / 0 - 40 / 4.3 – 4.7 / Live - 100 / 24
V / Rh / 50 / 224-Auto / ---- / 0 - 40 / 4.7 – 5.1 / Live - 100 / 24
Cr / Rh / 50 / 224-Auto / ---- / 0 - 40 / 5.2 – 5.6 / Live - 100 / 24
Mn / Rh / 50 / 224-Auto / ---- / 0 - 40 / 5.7 – 6.1 / Live - 100 / 24
Fe / Rh / 50 / 224-Auto / ---- / 0 - 40 / 6.2 – 6.6 / Live - 100 / 24
Co / Rh / 50 / 224-Auto / ---- / 0 - 40 / 6.7 – 7.1 / Live - 100 / 24
Ni / Rh / 50 / 224-Auto / ---- / 0 - 40 / 7.3 – 7.7 / Live - 100 / 24
Cu / Rh / 50 / 224-Auto / ---- / 0 - 40 / 7.8 – 8.2 / Live - 100 / 24
Zn / Rh / 50 / 224-Auto / ---- / 0 - 40 / 8.4 – 8.8 / Live - 100 / 24
Ga / Rh / 50 / 224-Auto / ---- / 0 - 40 / 9.0 – 9.5 / Live - 100 / 24
Ge / Rh / 50 / 224-Auto / ---- / 0 - 40 / 9.6 – 10.1 / Live - 100 / 24
As / Rh / 50 / 224-Auto / ---- / 0 - 40 / 11.5 – 12.0 / Live - 100 / 24
Se / Rh / 50 / 224-Auto / ---- / 0 - 40 / 11.0 – 11.5 / Live - 100 / 24
Rb / Rh / 50 / 224-Auto / ---- / 0 - 40 / 13.1 – 13.6 / Live - 100 / 24
Sr / Rh / 50 / 224-Auto / ---- / 0 - 40 / 13.9 – 14.4 / Live - 100 / 24
Y / Rh / 50 / 224-Auto / ---- / 0 - 40 / 14.7 – 15.2 / Live - 100 / 24
Zr / Rh / 50 / 224-Auto / ---- / 0 - 40 / 15.5 – 16.0 / Live - 100 / 24
Nb / Rh / 50 / 224-Auto / ---- / 0 - 40 / 16.3 – 16.8 / Live - 100 / 24
Mo / Rh / 50 / 224-Auto / ---- / 0 - 40 / 17.2 – 17.7 / Live - 100 / 24
Tc / Rh / 50 / 224-Auto / ---- / 0 - 40 / 18.1 – 18.6 / Live - 100 / 24
Ru / Rh / 50 / 224-Auto / ---- / 0 - 40 / 19.0 – 19.5 / Live - 100 / 24
Pd / Rh / 50 / 224-Auto / ---- / 0 - 40 / 20.7 – 21.5 / Live - 100 / 24
Ag / Rh / 50 / 224-Auto / ---- / 0 - 40 / 21.7 – 22.5 / Live - 100 / 24
Cd / Rh / 50 / 224-Auto / ---- / 0 - 40 / 22.7 – 23.5 / Live - 100 / 24
In / Rh / 50 / 224-Auto / ---- / 0 - 40 / 23.7 – 24.5 / Live - 100 / 24
Sn / Rh / 50 / 224-Auto / ---- / 0 - 40 / 24.8 – 25.6 / Live - 100 / 24
Sb / Rh / 50 / 224-Auto / ---- / 0 - 40 / 25.9 – 26.7 / Live - 100 / 24
Te / Rh / 50 / 224-Auto / ---- / 0 - 40 / 26.0 – 26.8 / Live - 100 / 24
Cs / Rh / 50 / 224-Auto / ---- / 0 - 40 / 4.1 – 4.5 / Live - 100 / 24
Ba / Rh / 50 / 224-Auto / ---- / 0 - 40 / 4.3 – 4.7 / Live - 100 / 24
Hf / Rh / 50 / 224-Auto / ---- / 0 - 40 / 7.7 – 8.1 / Live - 100 / 24
Ta / Rh / 50 / 224-Auto / ---- / 0 - 40 / 7.9 – 8.3 / Live - 100 / 24
W / Rh / 50 / 224-Auto / ---- / 0 - 40 / 8.2 – 8.6 / Live - 100 / 24
Re / Rh / 50 / 224-Auto / ---- / 0 - 40 / 8.5 – 8.9 / Live - 100 / 24
Os / Rh / 50 / 224-Auto / ---- / 0 - 40 / 8.7 – 9.1 / Live - 100 / 24
Ir / Rh / 50 / 224-Auto / ---- / 0 - 40 / 8.9 – 9.4 / Live - 100 / 24
Pt / Rh / 50 / 224-Auto / ---- / 0 - 40 / 9.2 – 9.7 / Live - 100 / 24
Au / Rh / 50 / 224-Auto / ---- / 0 - 40 / 9.5 – 10.0 / Live - 100 / 24
Hg / Rh / 50 / 224-Auto / ---- / 0 - 40 / 9.7 – 10.2 / Live - 100 / 24
Tl / Rh / 50 / 224-Auto / ---- / 0 - 40 / 10.0 – 10.5 / Live - 100 / 24
Pb / Rh / 50 / 224-Auto / ---- / 0 - 40 / 12.4 – 12.9 / Live - 100 / 24
Bi / Rh / 50 / 224-Auto / ---- / 0 - 40 / 10.6 – 11.1 / Live - 100 / 24
Po / Rh / 50 / 224-Auto / ---- / 0 - 40 / 10.9 – 11.4 / Live - 100 / 24

Quantitative Result

Analyte / Result / Std.Dev. / Proc.-Calc. / Line / Int.(cps/uA)
SiO2 / 8.440 % / [ 0.003] / Quan-EC / SiKa / 0.0002
Co2 / 0.000 % / [ 0.000] / Quan-FP / CKa / 0.0003
N2O5 / 0.000 % / [ 0.000] / Quan-FP / NKa / 0.0010
O / 91.560 % / [160.045] / Quan-FP / OKa / 0.0002
Na2O / 0.000 % / [ 0.000] / Quan-FP / NaKa / 0.0019
MgO / 0.000 % / [ 0.000] / Quan-FP / MgKa / 0.0045
Al2O3 / 0.000 % / [ 0.000] / Quan-FP / AlKa / 0.0003
P2O5 / 0.000 % / [ 0.000] / Quan-FP / PKa / 0.0001
SO3 / 0.000 % / [ 0.000] / Quan-FP / SKa / 0.0010
K2O / 0.000 % / [ 0.000] / Quan-FP / KKa / 0.0009
CaO / 0.000 % / [ 0.000] / Quan-FP / CaKa / 0.0007
Sc2O3 / 0.000 % / [ 0.000] / Quan-FP / ScKa / 0.0135
TiO2 / 0.000 % / [ 0.000] / Quan-FP / TiKa / 0.0000
V2O5 / 0.000 % / [ 0.000] / Quan-FP / VKa / 0.0078
Cr2O3 / 0.000 % / [ 0.000] / Quan-FP / CrKa / 0.0068
MnO / 0.000 % / [ 0.000] / Quan-FP / MnKa / 0.0332
Fe2)3 / 0.000 % / [ 0.000] / Quan-FP / FeKa / 0.0677
Co2O3 / 0.000 % / [ 0.000] / Quan-FP / CoKa / 0.371
NiO / 0.000 % / [ 0.000] / Quan-FP / NiKa / 0.0189
CuO / 0.000 % / [ 0.000] / Quan-FP / CuKa / 0.0874
ZnO / 0.000 % / [ 0.000] / Quan-FP / ZnKa / 0.0136
Ga2O3 / 0.000 % / [ 0.000] / Quan-FP / GaKa / 0.0000
GeO2 / 0.000 % / [ 0.000] / Quan-FP / GeKa / 0.089
As2O3 / 0.000 % / [ 0.000] / Quan-FP / AsKb / 0.0112
SeO2 / 0.000 % / [ 0.000] / Quan-FP / SeKa / 0.0073
Rb2O / 0.000 % / [ 0.000] / Quan-FP / RbKa / 0.0085
SrO / 0.000 % / [ 0.000] / Quan-FP / SrKa / 0.0148
Y2O3 / 0.000 % / [ 0.000] / Quan-FP / YKa / 0.0165
ZrO2 / 0.000 % / [ 0.000] / Quan-FP / ZrKa / 0.0021
NbO / 0.000 % / [ 0.000] / Quan-FP / NbKa / 0.0077
MoO3 / 0.000 % / [ 0.000] / Quan-FP / MoKa / 0.0000
Tc / 0.000 % / [ 0.000] / Quan-FP / TcKa / 0.0056
RuO2 / 0.000 % / [ 0.000] / Quan-FP / RuKa / 1.7290
PdO / 0.000 % / [ 0.000] / Quan-FP / PdKa / 0.1370
Ag2O / 0.000 % / [ 0.000] / Quan-FP / AgKa / 0.1283
CdO / 0.000 % / [ 0.000] / Quan-FP / CdKa / 0.0185
In2O3 / 0.000 % / [ 0.000] / Quan-FP / InKa / 0.0037
SnO2 / 0.000 % / [ 0.000] / Quan-FP / SnKa / 0.0000
Sb2O / 0.000 % / [ 0.000] / Quan-FP / SbKa / 0.0083
TeO / 0.000 % / [ 0.000] / Quan-FP / TeKa / 0.0185
CsO2 / 0.000 % / [ 0.000] / Quan-FP / CsLa / 0.0037
BaO / 0.000 % / [ 0.000] / Quan-FP / BaLa / 0.0000
HfO2 / 0.000 % / [ 0.000] / Quan-FP / HfLa / 0.0873
Ta2O5 / 0.000 % / [ 0.000] / Quan-FP / TaLa / 0.0733
WO3 / 0.000 % / [ 0.000] / Quan-FP / WLa / 0.0214
ReO2 / 0.000 % / [ 0.000] / Quan-FP / ReLa / 0.0163
OsO4 / 0.000 % / [ 0.000] / Quan-FP / OsLa / 0.0066
Ir2O3 / 0.000 % / [ 0.000] / Quan-FP / IrLa / 0.0026
PtO2 / 0.000 % / [ 0.000] / Quan-FP / PtLa / 0.0010
Au2O / 0.000 % / [ 0.000] / Quan-FP / AuLa / 0.0082
HgO / 0.000 % / [ 0.000] / Quan-FP / HgLa / 0.0136
Tl2O3 / 0.000 % / [ 0.000] / Quan-FP / TlLa / 0.0143
PbO / 0.000 % / [ 0.000] / Quan-FP / PbLb1 / 0.0116
Bi2O3 / 0.000 % / [ 0.000] / Quan-FP / BiLa / 0.0175
Po / 0.000 % / [ 0.000] / Quan-FP / PoLa / 0.0090