Primersa / Sequence (5’ to 3’)b / Positionc / Target
gumDF / ccgccggaattcGACCATGGTTGTGGTGTG (EcoRI) / 3414107–3414090 / gumD
gumDR / ccgccggaattcATGCGGATATCCAGCCAC (EcoRI) / 3412823–3412806 / gumD
gumMF / ctagtctagaGAGAAGCAGGATCTGAGC (XbaI) / 3403974–3403957 / gumM
gumHF1 / ccggaattcCGCATACGTTGGTCTTCC (EcoRI) / 3409376–3409359 / gumH
gumHR1 / gggcccaagcttGCTGCTTCATGCACGATG (HindIII) / 3408745–3408728 / gumH
gumHF2 / gggcccaagcttCACTGATGCAGCAAGCGC (HindIII) / 3408334–3408317 / gumH
gumHR2 / ccggaattcTCGCATTGATGCGGATCC (EcoRI) / 3407588–3407571 / gumH
gumKF1 / ccggaattcCAGGCCATCGTCAATTTCG (EcoRI) / 3406348–3406330 / gumK
gumKR1 / gggcccaagcttCGGACAAGACCAGATAGCA (HindIII) / 3405318–3405300 / gumK
gumKF2 / gggcccaagcttTCTTCGAAAGTGGCATCGC (HindIII) / 3404978–3404960 / gumK
gumKR2 / ccggaattcGATCTCCCATATTCACGCC (EcoRI) / 3404049–3404031 / gumK
gumIF / gggcccaagcttATCAAGTGCAGGCGCTTG (HindIII) / 3408148–3408131 / gumI
gumIR / gggcccaagcttGCCTTCATACAGGAACACC (HindIII) / 3407116–3407098 / gumI
gumFF1 / ctagtctagaACCGTTACTTCGATAGCGG (XbaI) / 3411707–3411689 / gumF
gumFR1 / gggcccaagcttCGACACGTAGATCAGCAAGG (HindIII) / 3410736–3410717 / gumF
gumFF2 / gggcccaagcttGTATTGTCCGGTGTTGCG (HindIII) / 3410452–3410435 / gumF
gumFR2 / ctagtctagaGGAAGACCAACGTATGCG (XbaI) / 3409376–3409359 / gumF
gumGF1 / ctagtctagaGTTGGCATTAGCGGTAGC (XbaI) / 3410669–3410652 / gumG
gumGR1 / gggcccaagcttCAGTGTCAAGCTGATCGC (HindIII) / 3409758–3409741 / gumG
gumGF2 / gggcccaagcttGCCGTGTTTATGCTGGTC (HindIII) / 3409494–3409477 / gumG
gumGR2 / ctagtctagaAAAAACCCGCCATGCGTG (XbaI) / 3408777–3408760 / gumG
gumLF / ccggaattcGCTATGGCGATAACGTCG (EcoRI) / 3404579–3404562 / gumL
gumLR / ccggaattcGTATTGGCGAAGAACACCC (EcoRI) / 3403165–3403147 / gumL
gumLR1 / gggcccaagcttGGATCGCCATACACCTCC (HindIII) / 3403777–3403760 / gumL
gumLF2 / gggcccaagcttGTGTCTTCAGTTGGGCGG (HindIII) / 3403420–3403403 / gumL
gumLR2 / ggtgttgatatcAACACCAGCAGATCCCAG (EcoRV) / 3402594–3402577 / gumL
gumBF1 / ctagctagtctagaCTTCGTGACTACGTCTGC (XbaI) / 3416987–3416970 / gumB
gumBR1 / cccgggaagcttCTCACCAACGCTCATGCC (HindIII) / 3416301–3416284 / gumB
gumBF2 / cccgggaagcttGCCAATCTGACCCTCCAG (HindIII) / 3416142–3416125 / gumB
gumBR2 / ctagctagtctagaGTCCAGCAACGTATCGGT (XbaI) / 3415414–3415397 / gumB
gumCF / ctagctagtctagaGAGAAAATGGTGGCCGAC (XbaI) / 3416280–3416263 / gumC
gumCR / ctagctagtctagaGATTCGGTAAGCGACCAG (XbaI) / 3414085–3414068 / gumC
gumEF / cccaagcttATGAGAGAGGAAACTCGC (HindIII) / 3412662–3412645 / gumE
gumER / cccaagcttAACCGCAGACAAACCACC (HindIII) / 3411506–3411489 / gumE
gumJF / ccggaattcCGGCATCATTCTGTTGGC (EcoRI) / 3406802–3406785 / gumJ
gumJR / ccggaattcTCCAGGTTGTGATCCACG (EcoRI) / 3404759–3404732 / gumJ
TrrnBF / actccatcgatGCTGTTTTGGCGGATGAGAG (ClaI) / - / TrrnB
TrrnBR / ccggaattcAAAAGGCCATCCGTCAGG (EcoRI) / - / TrrnB

a F and R represent forward and reverse primer, respectively.

b Underlined sequences indicate restriction sites for restriction enzymes as shown in parentheses.

c Numerical position on the X. oryzae KACC10331 genome (GenBank accession no. AE013598).