Asexual Reproduction Microscope Lab

Name ______Date ______

Mrs. Geithner-Marron (Bio 200) Period ______

Asexual Reproduction Microscope Lab

Objective: To observe various methods of asexual reproduction using a light microscope.

Part I: Budding

Materials:

Name ______Date ______

Mrs. Geithner-Marron (Bio 200) Period ______

·  glass slides

·  cover slips

·  droppers

·  yeast suspension (with sugar)

·  prepared slides of Hydra budding

Name ______Date ______

Mrs. Geithner-Marron (Bio 200) Period ______

Procedure for budding in yeast:

1.  Make a wet mount from yeast in sugar-water. (Note: Only a very small drop is needed.)

2.  Find the cells under low power, then switch to medium and refocus, then switch to high and refocus again. (Remember: only use the fine adjustment knob when on medium or high powers.)

3.  Observe each mount under high power for several minutes. (Note: If you have trouble seeing individual cells, add a drop of water to dilute the mount and blot excess water with a paper towel.)

4.  After you see evidence of budding under high power, draw a few cells from each mount in the circles below and on the next page.

5.  Label the parent cell & the offspring bud.

Procedure for budding in Hydra:

1.  View a prepared slide of Hydra budding under low power.

2.  Draw the Hydra & its offspring bud below.

3.  Label the parent Hydra & the offspring bud.

Questions:

1.  Describe the process of budding.

2.  What is the name used to describe budding in plants?

Part II: Binary Fission

Materials:

·  prepared slide of Paramecium undergoing fission

Procedure:

1.  View a prepared slide of Paramecium.

2.  Find the cells under low power, then switch to medium and refocus, then switch to high and refocus again. (Remember: only use the fine adjustment knob when on medium or high powers.)

3.  After you see evidence of fission under high power, draw the Paramecium (undergoing fission) in the circle below.

4.  Label each (newly forming) Paramecium, the cleavage furrow (indentation) where the cells are splitting, and the nucleus in each cell.

Questions:

1.  A Paramecium is a Protist. Are Paramecia (and other Protists) prokaryotic or eukaryotic?

2.  How is the process of binary fission in bacteria different from binary fission in Paramecia? (What process must Paramecia carry out before undergoing binary fission that bacteria do not have to carry out?) Why is it different?