Supplementary

Additional Supporting Information may be found in the online version of this article:

Table S1Tentative assignment of biochemical bands identified by FT-IR spectroscopy

Wavenumber(cm-1) / Assignments.(6, 14, 32, 45) / Biomolecules / Medium
2959-2953 / ν(CH3) membrane phospholipids / Phospholipids, lipids proteins / L50F50
2945-2898 / ν(CH2) membrane phospholipids / Phospholipids, lipids proteins / L50F50
2860-2844 / νs(CH2) membrane phospholipids / Phospholipids, lipids proteins / L50F50
1764-1720 / ν (CO) esters / Phospholipids, lipids proteins / L50F50
L20F100
1635-1610 / Amide I (β-structure) / Protein / L100F20
1535-1512 / Amide II , β structure / Protein / L100F20
1182-1037 / νa(COOC) phospholipids/cholesterol esters; νs(PO2−) nucleic acids / Phosphate,
carbohydrate / L20F100

= stretch, vs = symmetric, va = antisymmetric.

Table S2Comparison of cultivation parameters of S.oneidensis MR-1in batch cultures

Culture / Sampling OD600 / Lactate remaining(mM) / Fumarate Remaining(mM)
L100F20 / 0.15±0.02 / 83.53±2.51 / ND
L80F40 / 0.15±0.03 / 53.25±1.64 / ND
L70F50 / 0.13±0.02 / 37.98±0.52 / ND
L60F60 / 0.21±0.05 / 19.41±2.84 / 4.98±2.56
L50F70 / 0.19±0.05 / 5.35±2.54 / 14.42±3.32
L40F80 / 0.25±0.05 / 3.15±0.24 / 27.33±1.85
L20F100 / 0.32±0.05 / 1.40±0.08 / 73.18±11.01

ND: Not Detected

Metabolites definitely identified / Metabolites putatively annotated
2-hydroxyglutaric acid / 3-methyl-2-hydroxybutanoic acid
2-oxoglutaric acid / Acetylthiourea
Alanine / Ethanolamine
aspartic acid / glutamic acid and/or glutamine
citric acid / glutamic acid and/or pyroglutamic acid
fumaric acid / glycerol-2-phosphate and/or glycerol-3-phosphate
Glucose / heptadecanoic acid
glycerol / lactic acid dimer
Glycine / mannose and/or glucose
hexadecanoic acid / Monopalmitin
hexadecenoic acid / Monostearin
lactic acid / ribose-5-phosphate
leucine / sugar phosphate (glucose or fructose phosphate)
malic acid / tridecanoic acid, methyl ester
octadecanoic acid / Uracil
octadecenoic acid
pentadecanoic acid
Phosphate
Putrescine
pyruvic acid
succinic acid
valine

Table S3 List of annotated or identified metabolites identified by GC-MS.

Fig S1.PCA scores plot for IR data showing of cells phenotypic variation caused by dilution rates in the L100F20 medium

The different symbols represent the different sample points; same symbols represent the three analytic repeats.

Fig.S2 Impact of e--donor to e--acceptor ratio on individual cell morphological changes

Fig.S3 Comparison of differences in metabolite concentrations over different lactate: fumarate ratios, L70F50, L60F60, L50F70 and L40F80

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