Supplement 1: Supplementary Tables

Supplementary Table S1AGP sequences that were used as queries to identify centaury AGP sequences

Query sequences / Identified centaury sequence
GenBank
accessions / Species / Description
DR176757 / Gossypium
hirsutum / EST031 cotton fiber suppression subtractive hybridization Library - cDNA clone F204F7 / CeAGP1
EF672642 / fasciclin-like arabinogalactan protein 16 (FLA16) mRNA, complete cds / CeAGP 2
EF672640.1 / fasciclin-like arabinogalactan protein 14 (FLA14) mRNA, complete cds
NM_116029 / Arabidopsis thaliana / arabinogalactan protein 20 mRNA, complete cds / CeAGP 3
NM_124704 / arabinogalactan protein 22 mRNA, complete cds
XM_002270390 / Vitis
vinifera / PREDICTED: fasciclin-like arabinogalactan protein 10-like, mRNA / CeAGP 4
NM_001154084 / Zea
mays / arabinogalactan protein (LOC100281165), mRNA / CeAGP 5
NM_001155433 / arabinogalactan protein (LOC100282525), mRNA
EU961273 / clone 234180 arabinogalactan protein mRNA, complete cds

Supplementary Table S2Primers for qPCR of the CeAGP transcripts and centaury 18S rRNA

Gene / GenBank accession / Sequence (5′➔3′) / Amplicon, bp
CeAGP1 / KC733882 / F: GAC CGT TTG GAA TTG AGA CA / 166
R: ACT CAA CAA CTC AAA CTC CG
CeAGP2 / KC733883 / F: CAA ATA CGC GGA TCC ATC AT / 157
R: GAT TCC GGA GTA TCA AAC GG
CeAGP3 / KC733884 / F: CCA TTT ATG CCT GAG CAG TT / 153
R: CTT CTA ATG CTG GTG GCT TT
CeAGP4 / KC733885 / F: AGA TCT CGG TCA CGT GAA TA / 171
R: GTT AAC AAT CCG GGA GCA AT
CeAGP5 / KC733886 / F: TCT CAG CAC GAA CAT AAA GC / 160
R: GAC GGT GTG GAA TTT GAG AT
18S rRNA / FR863631 / F: TGA CGG AGA ATT AGG GTT CG / 190
R: CAA TGG ATC CTC GTT AAG GG

F – forward primer, R – reverse primer

Supplementary Table S3Amino acid composition of centaury AGP proteins

The amino acid (AA) composition is presented for pre-proteins (either complete or partial) and, where possible, for mature proteins (-N or -N-C) without fasciclin domains (-FAS), in order to evaluate the AA composition of the remaining AGP domains. Unk – unknown residue