Genotype / Number of flies / Rhythmic flies (%) / Average period of the group (hr)
cry-GAL4/+ / 16 / 93.75 / 24.3
cry-GAL4/UAS-hid / 16 / 0 / AR
cry-GAL4/UAS-hid/cry-GAL80I 3a / 16 / 62.5 / 23.8
cry-GAL4/UAS-hid/cry-GAL80II 4b / 16 / 75 / 24.5
cry-GAL4/UAS-hid/cry-GAL80II 8a / 8 / 37.5 / 24
cry-GAL4/UAS-hid/cry-GAL80II 5a / 16 / 93.75 / 24
cry-GAL4/UAS-hid/cry-GAL80II 3f1 / 14 / 85.71 / 24.3
cry-GAL4/UAS-hid/cry-GAL80II 8d / 16 / 100 / 24.5
cry-GAL4/UAS-hid/cry-GAL80II 8e / 16 / 25 / 24.3
cry-GAL4/UAS-hid/cry-GAL80II 3b / 10 / 20 / 25.6
cry-GAL4/UAS-hid/cry-GAL80II 8c / 16 / 31.25 / 23.3
cry-GAL4/UAS-hid/cry-GAL80II 6 / 16 / 0 / AR
cry-GAL4/UAS-hid/cry-GAL80II 2c / 12 / 0 / AR
cry-GAL4/UAS-hid/cry-GAL80II 3d / 8 / 0 / AR
cry-GAL4/UAS-hid/cry-GAL80III 3k / 7 / 28.57 / 24.5
cry-GAL4/UAS-hid/cry-GAL80III 3m / 25 / 88 / 24
cry-GAL4/UAS-hid/cry-GAL80III 3c / 24 / 79.16 / 24
cry-GAL4/UAS-hid/cry-GAL80III 2e / 22 / 90.90 / 24.3
cry-GAL4/UAS-hid/cry-GAL80III 3f2 / 16 / 81.25 / 24.5
cry-GAL4/UAS-hid/cry-GAL80III 4a / 16 / 12.5 / 25.8
cry-GAL4/UAS-hid/cry-GAL80III 1 / 16 / 0 / AR
cry-GAL4/UAS-hid/cry-GAL80III 7a / 9 / 0 / AR
cry-GAL4/UAS-hid/cry-GAL80II 8d3f1 * / 32 / 96.87 / 24
cry-GAL4/UAS-hid/cry-GAL80III 2e3m * / 32 / 96.87 / 24.5
cry-GAL4/UAS-hid/cry-GAL80III 3c3m * / 32 / 84.37 / 24

Supplementary Table S3.doc 2004-03-17050

Supplementary Table S3. Rescue of cry-GAL4/UAS-hid behavioral phenotype by cry-GAL80.

20 independent cry-GAL80 transgenic lines (eleven on chromosome III, eight on chromosome II and one on the X) were tested for their ability to suppress the behavioral defect induced by GAL4-mediated ablation of CRY+ neurons.

* Transgenic flies with two copies of cry-GAL80 insert were generated by standard recombination techniques. Three of the recombinant double insert lines (8d3f1, 2e3m and 3c3m) fully rescued the cry-GAL4; UAS-hid defect. The 2e3m line was used for the experiments described in the main body of the paper.